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Team:DTU-Denmark/Notebook/15 August 2013
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* Nir2 USER touchdown negative | * Nir2 USER touchdown negative | ||
* 1 kb ladder | * 1 kb ladder | ||
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| + | [[File:2013-08-15 nir2 user.jpg|600px]] | ||
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| + | The purification has the wrong length for Nir2 and was therefore discarded. | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 14:13, 15 August 2013
15 August 2013
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Contents |
lab 208
Main purpose
- Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBAD::RFP.
Who was in the lab
Ariadni, Helen, Kristian, Julia
Procedure
Plasmid isolation
Plasmid have been isolated following the protocol provided by ORIGENE PowerPrep HP Plasmid Miniprep Kit.
Glycerol stock cultures
-80C stock solution of Sec2, TAT3-1a, TAT3-2
Results
- 1 kb ladder
- purification of the extraction fragment of Nir2
- Nir2 USER touchdown, sample 1
- Nir2 USER touchdown, sample 2
- Nir2 USER touchdown, sample 3
- Nir2 USER touchdown, sample 4
- Nir2 USER touchdown, sample 5
- Nir2 USER touchdown negative
- 1 kb ladder
The purification has the wrong length for Nir2 and was therefore discarded.
Conclusion
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