Team:DTU-Denmark/Methods/Making anoxic conditions

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Method: Injection of N<sub>2<sub> to achieve anaerobic environment
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==Sparging with N<sub>2</sub> ==
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Procedure:
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1. Put carefully one long needle in the vessel for injecting N2
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2. Put one short needle in the vessel for removing the containing oxygen
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3. Open the pressure valve of the Nitrogen gas container
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4. Turn round (anticlockwise) the nitrogen valve to open it until there is a flow of gas.
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5. Sparge with N2 by connecting the N2 gas tube with the long needle.
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6. After 3/ 5 min remove first the small needle and after 2 seconds the long needle.
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7. Turn off the nitrogen valve.
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8. Turn off the pressure valve.
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Method: Injection of N<sub>2</sub> to achieve anaerobic environment
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*Procedure
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# Put carefully one long needle in the vessel for injecting N<sub>2</sub>
 +
# Put one short needle in the vessel for removing the containing oxygen
 +
# Open the pressure valve of the Nitrogen gas container
 +
# Turn round (anticlockwise) the nitrogen valve to open it until there is a flow of gas.
 +
# Sparge with N<sub>2</sub> by connecting the N<sub>2</sub> gas tube with the long needle.
 +
# After 3/ 5 min remove first the small needle and after 2 seconds the long needle.
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# Turn off the nitrogen valve.
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# Turn off the pressure valve.
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<div style="clear: both;"></div>
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</div>
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 17:51, 15 August 2013

Sparging with N2

Sparging with N2

Method: Injection of N2 to achieve anaerobic environment

  • Procedure
  1. Put carefully one long needle in the vessel for injecting N2
  2. Put one short needle in the vessel for removing the containing oxygen
  3. Open the pressure valve of the Nitrogen gas container
  4. Turn round (anticlockwise) the nitrogen valve to open it until there is a flow of gas.
  5. Sparge with N2 by connecting the N2 gas tube with the long needle.
  6. After 3/ 5 min remove first the small needle and after 2 seconds the long needle.
  7. Turn off the nitrogen valve.
  8. Turn off the pressure valve.

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