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Team:UNITN-Trento/Notebook/Labposts/06/14
From 2013.igem.org
(Difference between revisions)
(Created page with "{ "date" : "2013-08-05", "author" : "viola", "title" : "2nd ligation", "content" : "After the negative screening of Pedro's inocula of the ligation of EFE + s04617 today I re...") |
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{ | { | ||
| - | "date" : "2013- | + | "date" : "2013-06-06", |
| - | "author" : " | + | "author" : "caterina-michele-fabio" |
| - | "title" : " | + | "title" : "We have the parts!", |
| - | "content" : " | + | "content" : "<html> |
| - | "tags" : " | + | We performed the purification of <b>BBa_J45320</b>, <b>BBa_J45119</b>, <b>BBa_J45700</b> (obtained from NEB10β colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image. </html> |
| + | {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/f/f2/Tn-2013-Digestion_EcoRI_PstI.jpg" style ="width: 450px"> | ||
| + | </center></html>}}<html><br/> | ||
| + | </html>", | ||
| + | "tags" : "PchB-PchBA-BSMT1" | ||
} | } | ||
Revision as of 12:14, 19 August 2013
{ "date" : "2013-06-06", "author" : "caterina-michele-fabio" "title" : "We have the parts!", "content" : " We performed the purification of BBa_J45320, BBa_J45119, BBa_J45700 (obtained from NEB10β colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image.
", "tags" : "PchB-PchBA-BSMT1" }
