Team:UNITN-Trento/Notebook/Labposts/06/14

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(Difference between revisions)

Revision as of 12:27, 19 August 2013

{ "date" : "2013-06-06", "author" : "caterina-michele-fabio" "title" : "We have the parts!", "content" : " We performed the purification of BBa_J45320, BBa_J45119, BBa_J45700 (obtained from NEB10β colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image.

Gel Image

", "tags" : "PchB-PchBA-BSMT1" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-05",
+	"date" : "2013-06-06",
-	"author" : "viola",
+	"author" : "caterina-michele-fabio"
-	"title" : "2nd ligation",
+	"title" : "We have the parts!",
-	"content" : "After the negative screening of Pedro's inocula of the ligation of EFE + s04617 today I retried this cloning.",
+	"content" : "<html>	We performed the purification of <b>BBa_J45320</b>, <b>BBa_J45119</b>, <b>BBa_J45700</b> (obtained from NEB10&beta; colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center>		<img src="https://static.igem.org/mediawiki/2013/f/f2/Tn-2013-Digestion_EcoRI_PstI.jpg" style ="width: 450px">	</center></html>}}<html><br/></html>",
-	"tags" : "s04617-EFE"
+	"tags" : "PchB-PchBA-BSMT1"
 }