Team:UNITN-Trento/Notebook/Labposts/08/39

From 2013.igem.org

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{
{
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"date" : "2013-08-15",
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    "date" : "2013-08-15",
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"author" : "emil",
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    "author" : "emil",
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"title" : "Emil's(re)begins",
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    "title" : "Emil's(re)begins",
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"content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>
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    "content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>     {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>         <center>           <table>               <td>                   <th>Sample</th>                   <th>Quantities</th>               </td>               <td>                   <tr>GFP 1</tr>                   <tr>70.5 ng/6micro;l</tr>               </td>               <td>                   <tr>GFP 2</tr>                   <tr>52 ng/6micro;l</tr>               </td>                 <td>                   <tr>PXyl 1</tr>                   <tr>110.4 ng/6micro;l</tr>               </td>                 <td>                   <tr>Pxyl 2</tr>                   <tr>77.7 ng/6micro;l</tr>               </td>                 <td>                   <tr>Pxyl 3</tr>                   <tr>137 ng/6micro;l</tr>               </td>             </table>           </center>       </html>}}   <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
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      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>
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    "tags" : "PXyl-GFP"
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        <center>
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            <table>
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                <td>
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                  <th>Sample</th>
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                  <th>Quantities</th>
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                </td>
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                <td>
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                    <tr>GFP 1</tr>
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                    <tr>70.5 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>GFP 2</tr>
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                    <tr>52 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>PXyl 1</tr>
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                    <tr>110.4 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>Pxyl 2</tr>
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                    <tr>77.7 ng/6micro;l</tr>
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                </td>
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                <td>
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                    <tr>Pxyl 3</tr>
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                    <tr>137 ng/6micro;l</tr>
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                </td>
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            </table>
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          </center>
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        </html>}}
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    <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
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"tags" : "PXyl-GFP"
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}
}

Revision as of 07:21, 20 August 2013

{ 

   "date" : "2013-08-15",
   "author" : "emil",
   "title" : "Emil's(re)begins",
   "content" : "I screened 5µl of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the     results:      
gel image
 
Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the purification protocol with the following results:      
results
         
            GFP 170.5 ng/6micro;lGFP 252 ng/6micro;lPXyl 1110.4 ng/6micro;lPxyl 277.7 ng/6micro;lPxyl 3137 ng/6micro;l
                
                   Sample                   Quantities                                                    
                    
                                
                    
                    
                                 
                    
                    
                                 
                    
                    
                                 
                    
                    
                             
           
        
    After that I did a digestion overnight of 3 µl of the GFP1 and the PXyl1 following the  digestion protocol.",
   "tags" : "PXyl-GFP"

}

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/08/39"