Team:Groningen/Labwork/13 August 2013

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Did a ligation reaction between Pdes and CheY.
Did a ligation reaction between Pdes and CheY.
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Did a ligation reaction between Pdes-CheY, BBa_k823823 and GFP/BBa_E0030 (eYFP)
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<br>Did a ligation reaction between Pdes-CheY, BBa_k823823 and GFP/BBa_E0030 (eYFP)
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<br>Transformation to <i>E. coli</i>.
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<br>Plated &Delta;Chey and &Delta;Des.
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<h2>Inne & Sander</h2>
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The results of the motility assay were inconclusive let, so the tubes were put back in the 37C incubator.
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<H2>Sebas</h2>
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Grew B. sub wt, hyperspank::GFP 1&2 and induced at OD=~0,45 with 1mM IPTG. Checked T1, T2 and T3 under the <Br>microscope, no significant differance in fluorescence was detected between the WT and the mutants, indicating <Br>that GFP does not work in Bacillus or that the promoter does not work.
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Latest revision as of 09:50, 20 August 2013

Mirjam

Did a ligation reaction between Pdes and CheY.
Did a ligation reaction between Pdes-CheY, BBa_k823823 and GFP/BBa_E0030 (eYFP)
Transformation to E. coli.
Plated ΔChey and ΔDes.

Inne & Sander

The results of the motility assay were inconclusive let, so the tubes were put back in the 37C incubator.

Sebas

Grew B. sub wt, hyperspank::GFP 1&2 and induced at OD=~0,45 with 1mM IPTG. Checked T1, T2 and T3 under the
microscope, no significant differance in fluorescence was detected between the WT and the mutants, indicating
that GFP does not work in Bacillus or that the promoter does not work.