Team:UNITN-Trento/Notebook/Labposts/08/39

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(Difference between revisions)
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     "date" : "2013-08-15",
     "date" : "2013-08-15",
     "author" : "emil",
     "author" : "emil",
-
     "title" : "Emil's(re)begins",
+
     "title" : "Emil's (re)begins",
     "content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>        <center>            <table>                <td>                  <th>Sample</th>                  <th>Quantities</th>                </td>                <td>                    <tr>GFP 1</tr>                    <tr>70.5 ng/6micro;l</tr>                </td>                <td>                    <tr>GFP 2</tr>                    <tr>52 ng/6micro;l</tr>                </td>                <td>                    <tr>PXyl 1</tr>                    <tr>110.4 ng/6micro;l</tr>                </td>                <td>                    <tr>Pxyl 2</tr>                    <tr>77.7 ng/6micro;l</tr>                </td>                <td>                    <tr>Pxyl 3</tr>                    <tr>137 ng/6micro;l</tr>                </td>            </table>          </center>        </html>}}    <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
     "content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>        <center>            <table>                <td>                  <th>Sample</th>                  <th>Quantities</th>                </td>                <td>                    <tr>GFP 1</tr>                    <tr>70.5 ng/6micro;l</tr>                </td>                <td>                    <tr>GFP 2</tr>                    <tr>52 ng/6micro;l</tr>                </td>                <td>                    <tr>PXyl 1</tr>                    <tr>110.4 ng/6micro;l</tr>                </td>                <td>                    <tr>Pxyl 2</tr>                    <tr>77.7 ng/6micro;l</tr>                </td>                <td>                    <tr>Pxyl 3</tr>                    <tr>137 ng/6micro;l</tr>                </td>            </table>          </center>        </html>}}    <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
     "tags" : "PXyl-GFP"
     "tags" : "PXyl-GFP"
}
}

Revision as of 10:22, 20 August 2013

{ 

   "date" : "2013-08-15",
   "author" : "emil",
   "title" : "Emil's (re)begins",
   "content" : "I screened 5µl of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the     results:      
gel image
 
Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the purification protocol with the following results:      
results
         
            GFP 170.5 ng/6micro;lGFP 252 ng/6micro;lPXyl 1110.4 ng/6micro;lPxyl 277.7 ng/6micro;lPxyl 3137 ng/6micro;l
                
                   Sample                   Quantities                                                    
                    
                                
                    
                    
                                 
                    
                    
                                 
                    
                    
                                 
                    
                    
                             
           
        
    After that I did a digestion overnight of 3 µl of the GFP1 and the PXyl1 following the  digestion protocol.",
   "tags" : "PXyl-GFP"

}

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