Team:KAIST Korea/

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ul.oe_menu2 > li > a:hover,
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ul.oe_menu2 div{
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ul.oe_menu2 div ul li a{
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ul.oe_menu2 div ul.oe_full2{
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ul.oe_menu2 div ul li a:hover{
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ul.oe_menu2 li ul{
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#main #main-img
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#main a #main-logo
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{
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div .oe_wrapper{
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position:absolute;
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z-index:3;
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top:275px;
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}
 +
 +
div .oe_wrapper2{
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position:absolute;
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z-index:2;
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top:358px;
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}
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div .iGEMLogo{
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z-index:4;
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bottom: 80px;
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padding : 0 0 0 10px;
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}
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#menucontent
 +
{
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        text-align: right;
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font-family: 'Gloria Hallelujah', cursive;
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#top-img-description-box
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{
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position: relative;
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#horizontal-line
 +
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position: relative;
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 +
        left:292px;
 +
        top:-90px;
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        z-index:2;
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}
 +
div .contact-us{
 +
position: absolute;
 +
z-index:2;
 +
top:450px;
 +
padding:4px 0px 0px 10px;
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width:268px;
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color:white;
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font-weight:bold;
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}
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div .contact-us #contact-us-information{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
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}
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div .contact-us #contact-us-information a:hover{
 +
font-family: 'Source Sans Pro', sans-serif;
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font-size:12px;
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font-style: italic;
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color:#fcf967;
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font-weight:bold;
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text-decoration:none;
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div .contact-us #contact-us-information a{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
text-decoration:none;
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}
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div .contact-us #contact-us-title{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:22px;
 +
line-height:170%;
 +
color:#dddddd;
 +
height:30px;
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 +
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</style>
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<script type="text/javascript">
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  WebFontConfig = {
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 +
</head>
 +
 +
<body>
 +
<div id="main">
 +
<a href="https://2012.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
 +
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
 +
<div class="oe_wrapper">
 +
<ul id="oe_menu" class="oe_menu">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">HOME</a>
 +
<div style="top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">Introduction</a></li>
 +
</ul>
 +
<ul style="width:100px;margin:0px 5px 0px 5px;">
 +
<li style="font-size:14px;"><a href="https://2012.igem.org/Team:KAIST_Korea/Home_iGEM">iGEM</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Members">TEAM</a>
 +
<div style="left:-85px;top:25px;"><!-- -112px -->
 +
<ul>
 +
<li><a style="font-size:14px;"href="https://igem.org/Team.cgi?id=907">Official Team Profile</a></li>
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</ul>
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<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_KAIST">Members</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Gallery">Gallery</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">DATA</a>
 +
<div style="left:-182px;top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">Description</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_Bio">Bio Bricks</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="oe_wrapper2">
 +
<ul id="oe_menu2" class="oe_menu2">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">PROJECT</a>
 +
<div style="top:21px;left:32px;">
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">Overview</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Results">Results</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Future">Future Plan</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling">Modeling</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">NOTEBOOK</a>
 +
<div style="left:-91px;top:30px;"><!-- -112px -->
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">Labnote</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol">Protocol</a></li>
 +
</ul>
 +
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">PLUS</a>
 +
<div style="left:-152px;top:21px;">
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">Safety</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Human">Human Practice</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Attribution">Attribution</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Sponsor">Sponsors</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="contact-us">
 +
<div id="contact-us-title">2
-
<li><a href="https://dl.dropboxusercontent.com/u/202315040/igem/index.html">Home</a></li>
 
-
<li><a href="#">Team</a></li>
 
-
<li><a href="#">Project</a>
 
-
<!-- <ul>
 
-
<li><a href="#" class="documents">Documents</a></li>
 
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<li><a href="#" class="messages">Messages</a></li>
 
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<li><a href="#" class="signout">Sign Out</a></li>
 
-
</ul>
 
-
 
-
-->
 
-
 
-
</li>
 
-
<li><a href="#">Labnote</a></li>
 
-
<li><a href="#">Human Practice</a></li>
 
-
<li><a href="https://dl.dropboxusercontent.com/u/202315040/igem/safety.html">Safety</a></li>
 
-
</ul> <!-- end .menu -->
+
013 KAIST Korea</div>
 +
<div id="contact-us-information">
 +
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
 +
<b>Twitter</b> : <a href="http://twitter.com/KAIST_iGEM_2012">twitter.com/KAIST_iGEM_2012</a></br>
 +
<b>Facebook</b> : <a href="http://www.facebook.com/KAISTiGEM2012">www.facebook.com/KAISTiGEM2012</a>
 +
</div>
 +
</div>
 +
 +
<div class="iGEMLogo">
 +
<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/0/00/KAIST_iGEM_logo.png"/></a>
</div>
</div>
</div>
</div>
-
      <!-- </header> -->
+
        <!-- The JavaScript -->
-
 
+
        <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.3/jquery.min.js"></script>
-
 
+
        <script type="text/javascript">
-
 
+
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<h2>RAPTOR</h2>
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                            </section>
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-
<p>KAIST_Korea</p>
+
</script>
-
</div>
+
<script type="text/javascript">
-
<p class="button-style"><a href="#">GO TO ABSTRACT</a></p>
+
     
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<span id="little"><span id="starter">T</span>he widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
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<div class="row">
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iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The <span id="starter">RAPTOR</span> (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
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<p>The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
+
-
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
+
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
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Team:KAIST Korea/Project

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KAIST Korea 2013 iGEM


Project : Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.




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