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Team:DTU-Denmark/Notebook/22 August 2013
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{{:Team:DTU-Denmark/Templates/StartPage|22 August 2013}} | {{:Team:DTU-Denmark/Templates/StartPage|22 August 2013}} | ||
| - | =lab | + | =lab 208= |
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==Main purpose== | ==Main purpose== | ||
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==Who was in the lab== | ==Who was in the lab== | ||
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| + | Kristian, Henrike | ||
==Procedure== | ==Procedure== | ||
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Redid for HAO and cyc in arabinose inducible pZA21::araBAD and for Nir fragments | Redid for HAO and cyc in arabinose inducible pZA21::araBAD and for Nir fragments | ||
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| + | ===PCR for Biobrick parts=== | ||
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| + | Set up a new PCR reaction for Biobrick parts using HF buffer and 5% DMSO but no MgCl2. PCR was run on a touchdown program | ||
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| + | primers: 53a, 53b | ||
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| + | template: Sec2 miniprep | ||
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| + | |||
==Results== | ==Results== | ||
Revision as of 12:32, 22 August 2013
22 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Kristian, Henrike
Procedure
USER ligation and transformation
Redid for HAO and cyc in arabinose inducible pZA21::araBAD and for Nir fragments
PCR for Biobrick parts
Set up a new PCR reaction for Biobrick parts using HF buffer and 5% DMSO but no MgCl2. PCR was run on a touchdown program
primers: 53a, 53b
template: Sec2 miniprep
Results
Conclusion
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