Team:Paris Bettencourt/Notebook/Phage Sensor/Monday 29th July.html

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<h3>Monday 29<sup>th</sup> July</h3>
<h3>Monday 29<sup>th</sup> July</h3>
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Liquid culture of:<br>
Liquid culture of:<br>
pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture<br>
pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture<br>
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pSSB1 (from plate) - 5ml culture<br>
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pSSB1 (from plate) - 5 ml culture<br>
pSSB2 (from plate ) - 5 ml culture<br>
pSSB2 (from plate ) - 5 ml culture<br>
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Plating of addgene plasmids:<br>
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Plating of addgene bacteria containing the plasmids we want:<br>
streak out bacteria from addgene to get single colonies:<br>
streak out bacteria from addgene to get single colonies:<br>
pBAC-BA-lacZ<br>
pBAC-BA-lacZ<br>
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pCas9<br>
pCas9<br>
pCRIPSR::rpsL<br>
pCRIPSR::rpsL<br>
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Place your note here
 
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Latest revision as of 16:51, 22 August 2013

Phage Sensor

Monday 29th July


Liquid culture of:
pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture
pSSB1 (from plate) - 5 ml culture
pSSB2 (from plate ) - 5 ml culture

Plating of addgene bacteria containing the plasmids we want:
streak out bacteria from addgene to get single colonies:
pBAC-BA-lacZ
pCRISPR
pCas9
pCRIPSR::rpsL

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