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Team:Paris Bettencourt/Notebook/Phage Sensor/Monday 29th July.html
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Liquid culture of:<br> | Liquid culture of:<br> | ||
pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture<br> | pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture<br> | ||
| - | pSSB1 (from plate) - | + | pSSB1 (from plate) - 5 ml culture<br> |
pSSB2 (from plate ) - 5 ml culture<br> | pSSB2 (from plate ) - 5 ml culture<br> | ||
<br> | <br> | ||
Latest revision as of 16:51, 22 August 2013
Phage Sensor
ASDFMonday 29th July
Liquid culture of:
pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture
pSSB1 (from plate) - 5 ml culture
pSSB2 (from plate ) - 5 ml culture
Plating of addgene bacteria containing the plasmids we want:
streak out bacteria from addgene to get single colonies:
pBAC-BA-lacZ
pCRISPR
pCas9
pCRIPSR::rpsL
