Team:Groningen/Labwork/23 August 2013
From 2013.igem.org
(Difference between revisions)
(Created page with "<html> <head> <meta http-equiv="X-UA-Compatible" content="IE=EDGE" charset="utf-8" /> <link rel="stylesheet" href="https://2013.igem.org/Team:Groningen/CSS/DefaultPage?action=ra...") |
|||
Line 24: | Line 24: | ||
S15, S16 and BBa_K823023 were digested with EcoRI and BamHI. | S15, S16 and BBa_K823023 were digested with EcoRI and BamHI. | ||
<br>S3, S4, S9, S10 and BBa_K823023 were digested with BamHI and PstI. | <br>S3, S4, S9, S10 and BBa_K823023 were digested with BamHI and PstI. | ||
+ | |||
+ | <H2>Sebas</H2> | ||
+ | |||
+ | The 6 inoculated hy_spank-GFPdsm colonies grew overnight, preformed a miniprep (conc: >100ng/µl). Did a digestion check | ||
+ | <Br>with XbaI*PstI, expected sizes were: 759, 7544. | ||
+ | <Br>All colonies had the same sizes around ~750 and 7000~8000: | ||
+ | <br><img> </img> | ||
+ | |||
Revision as of 10:44, 23 August 2013
Claudio
S15, S16 and BBa_K823023 were digested with EcoRI and BamHI.S3, S4, S9, S10 and BBa_K823023 were digested with BamHI and PstI.
Sebas
The 6 inoculated hy_spank-GFPdsm colonies grew overnight, preformed a miniprep (conc: >100ng/µl). Did a digestion checkwith XbaI*PstI, expected sizes were: 759, 7544.
All colonies had the same sizes around ~750 and 7000~8000: