Team:Groningen/Labwork/23 August 2013

From 2013.igem.org

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The 6 inoculated hy_spank-GFPdsm colonies grew overnight, preformed a miniprep (conc: >100ng/µl). Did a digestion check  
The 6 inoculated hy_spank-GFPdsm colonies grew overnight, preformed a miniprep (conc: >100ng/µl). Did a digestion check  
<Br>with XbaI*PstI, expected sizes were: 759, 7544.
<Br>with XbaI*PstI, expected sizes were: 759, 7544.
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<Br>All colonies had the same sizes around ~750 and 7000~8000:
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<Br>All plasmid purification's had the same sizes around ~750 and 7000~8000:
<br><img src="https://static.igem.org/mediawiki/2013/5/56/F102471_2013-08-23_12hr_30min.jpg" width="300" height="400"></img>
<br><img src="https://static.igem.org/mediawiki/2013/5/56/F102471_2013-08-23_12hr_30min.jpg" width="300" height="400"></img>

Revision as of 10:50, 23 August 2013

Claudio

S15, S16 and BBa_K823023 were digested with EcoRI and BamHI.
S3, S4, S9, S10 and BBa_K823023 were digested with BamHI and PstI.

Sebas

The 6 inoculated hy_spank-GFPdsm colonies grew overnight, preformed a miniprep (conc: >100ng/µl). Did a digestion check
with XbaI*PstI, expected sizes were: 759, 7544.
All plasmid purification's had the same sizes around ~750 and 7000~8000: