Team:DTU-Denmark/Notebook/23 August 2013
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+ | | 10C || hold || - | ||
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+ | ===PCR constitutive reference promoter=== | ||
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+ | program: | ||
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+ | ! temperature !! time !! cycles | ||
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+ | | 98C || 2:00 || - | ||
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+ | | 98C || 0:20 || 36 | ||
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+ | | 56C || 1:00 || 36 | ||
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+ | | 72C || 3:00 || 36 | ||
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| 72C || 5:00 || - | | 72C || 5:00 || - |
Revision as of 15:45, 23 August 2013
23 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Kristian, Henrike
Procedure
PCR Biobrick Isolation
Used new primers to isolate Hello World constructs. Used HF buffer and tested three different concentrations of DMSO: 0%, 2% and 5%.
Primers: 56a, 56b
template: TAT2-1 (miniprep)
program:
temperature | time | cycles |
---|---|---|
98C | 2:00 | - |
98C | 0:20 | 36 |
56C | 0:30 | 36 |
72C | 0:45 | 36 |
72C | 5:00 | - |
10C | hold | - |
PCR constitutive reference promoter
program:
temperature | time | cycles |
---|---|---|
98C | 2:00 | - |
98C | 0:20 | 36 |
56C | 1:00 | 36 |
72C | 3:00 | 36 |
72C | 5:00 | - |
10C | hold | - |
Results
Gel electrophoresis
- 1 kb ladder
- Sec2 Biobrick
- Sec2 Biobrick
- Sec2 neg
- col AMO 1
- col AMO 2
- col AMO 3
- col AMO 4
- col AMO 5
- col AMO 6
- col AMO 7
- col AMO 8
- col AMO 9
- col AMO 10
- 1 kb ladder
- 1 kb ladder
- col Nir
- constitutive reference promoter
- constitutive reference promoter
- Biobrick Isolation TAT2-1 negative
- Biobrick Isolation TAT2-1 2% DMSO
- Biobrick Isolation TAT2-1 5% DMSO
- Biobrick Isolation TAT2-1 w/o DMSO
- 1 kb ladder
Conclusion
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