From 2013.igem.org
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| - | <html>
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| - | <div class ="tbnote">
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| - | <h2>Phage Sensor</h2>
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| - | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Phage_Sensor" target="_blank" class="tbnotelogo PSlogo"> ASDF </a>
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| - | <div style="clear: both;"></div>
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| - | <h3>Saturday 10<sup>th</sup> August</h3>
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| - | <p><b><em>
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| - | <!-- === Modify from here === -->
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| - | Test of M13 and Liquid culture
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| - | <!-- === To here === -->
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| - | </br></em></b></p>
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| - | <!-- === Modify from here === -->
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| - | <b>Test of M13</b><br>
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| - | <br>
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| - | 1) 100 μ l of plating bacteria per tube<br>
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| - | 2) Prepare tenfold serial dilutions (10-6 to 10-9 ) of the bacteriophage stock in LB<br>
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| - | 3) Put 10 μ l/ 100 μ l of each dilution to the bacteria<br>
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| - | 4) Mix the bacteriophage particles with the bacterial culture by vortexing gently.<br>
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| - | 5) Add 40 μ l of X-gal solution (working conc) and IPTG (working conc) solution to each of the tubes<br>
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| - | 6) Add 3ml of Agar to each tube and gently vortexing for 3 seconds<br>
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| - | 7) Pour the mixture onto plates containing LB medium supplemented with 5 mM MgCl2 <br>
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| - | 8) Swirl the plate gently to ensure an even distribution of bacteria and agar.<br>
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| - | 9) Incubate them at 37°C.<br>
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| - | <br>
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| - | <br>
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| - | <b>Liquid culture:</b><br>
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| - | E1010<br>
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| - | J04550<br>
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| - | <!-- === To here === -->
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| - | </div>
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| - | </html>
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Latest revision as of 17:24, 23 August 2013
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