lab 208

Main purpose

Who was in the lab

Kristian, Henrike

Procedure

Results

Gels

• 1 kb
• colony PCR NirG
• constitutive reference promoter, GC buffer
• constitutive reference promoter, GC buffer + 3% DMSO
• constitutive reference promoter, HF buffer
• constitutive reference promoter, HF buffer + 3% DMSO
• 1 kb

Gel could not be read and will be redone.

• 1 kb
• colony PCR cycAX, colony 2
• colony PCR cycAX, colony 3
• colony PCR cycAX, colony 4
• colony PCR cycAX, colony 6
• colony PCR cycAX, colony 7
• colony PCR cycAX, colony 8
• colony PCR cycAX, colony 9
• constitutive reference promoter, GC buffer
• constitutive reference promoter, GC buffer + 3% DMSO
• constitutive reference promoter, HF buffer
• constitutive reference promoter, HF buffer + 3% DMSO
• 1 kb

The insert is confirmed for the colonies 3, 4 and 8.

Conclusion

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