Team:Groningen/Labwork/26 August 2013

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<h2>Claudio</h2>
<h2>Claudio</h2>
Plates (S7, S8, S11, S12, S13, S14) showed roughly hundreds of colonies per plate. One colony per plate is tested using ColonyPCR with VF2 and VR primers.
Plates (S7, S8, S11, S12, S13, S14) showed roughly hundreds of colonies per plate. One colony per plate is tested using ColonyPCR with VF2 and VR primers.
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<br><br>Plates (S3, S4, S9 and S10) showed 1-2 colonies per plate. One colony per plate is inoculated in LB + Amp(<b>Sander</b>).
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<br><img src="https://static.igem.org/mediawiki/2013/7/7e/ColonyPCR-IDTgenes26082013.jpg" width="50%"></img>
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<br>The colonies contain the right construct.
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<br><br>Plates (S3, S4, S9 and S10) showed 1-2 colonies per plate. One colony per plate is inoculated in LB + Amp (<b>Sander</b>).
<br><br>Plates (S15 and S16) showed no colonies.
<br><br>Plates (S15 and S16) showed no colonies.
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<h2>Inne</h2>
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Poured plates to repeat the motility assay, 0.3% & 0.4% LB agar plates (20g/L LB). They are to dry overnight at the bench. The plates were made with a pipet, to ensure there is 13 ml in every plate.
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Latest revision as of 15:37, 27 August 2013

Claudio

Plates (S7, S8, S11, S12, S13, S14) showed roughly hundreds of colonies per plate. One colony per plate is tested using ColonyPCR with VF2 and VR primers.

The colonies contain the right construct.

Plates (S3, S4, S9 and S10) showed 1-2 colonies per plate. One colony per plate is inoculated in LB + Amp (Sander).

Plates (S15 and S16) showed no colonies.

Inne

Poured plates to repeat the motility assay, 0.3% & 0.4% LB agar plates (20g/L LB). They are to dry overnight at the bench. The plates were made with a pipet, to ensure there is 13 ml in every plate.