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Team:Paris Saclay/Notebook/July/3
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*Observed the Petri dish, we decided to perform a PCR test for the colonies from these 4 mediums. | *Observed the Petri dish, we decided to perform a PCR test for the colonies from these 4 mediums. | ||
*From those incubated plates, 4 colonies (2 from FNR+plasmid PSB1C3, 2 from LacZ and AmilCP) were selected in parallel for further test. | *From those incubated plates, 4 colonies (2 from FNR+plasmid PSB1C3, 2 from LacZ and AmilCP) were selected in parallel for further test. | ||
*Used 4 primers: VF2, VR, PFNR_up, PFNR_down for verification. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. The PCR products had been put on electrophoresis gel for the verification | *Used 4 primers: VF2, VR, PFNR_up, PFNR_down for verification. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. The PCR products had been put on electrophoresis gel for the verification | ||
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=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 02:28, 28 August 2013
Notebook : July 3
Summary:
- Observed the Petri dish, we decided to perform a PCR test for the colonies from these 4 mediums.
- From those incubated plates, 4 colonies (2 from FNR+plasmid PSB1C3, 2 from LacZ and AmilCP) were selected in parallel for further test.
- Used 4 primers: VF2, VR, PFNR_up, PFNR_down for verification. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. The PCR products had been put on electrophoresis gel for the verification
Lab work
constructing
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