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| | </style> | | </style> |
| | <body> | | <body> |
| - | <script>
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| - | // CREDITS:
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| - | // Snowmaker Copyright (c) 2003 Peter Gehrig. All rights reserved.
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| - | // Distributed by Hypergurl
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| - | // Permission given to use the script provided that this notice remains as is.
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| - |
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| - | // Set the number of snowflakes (more than 30 - 40 not recommended)
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| - | var snowmax=35
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| - |
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| - | // Set the colors for the snow. Add as many colors as you like
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| - | var snowcolor=new Array("#aaaacc","#ddddFF","#ccccDD")
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| - |
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| - | // Set the fonts, that create the snowflakes. Add as many fonts as you like
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| - | var snowtype=new Array("Arial Black","Arial Narrow","Times","Comic Sans MS")
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| - |
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| - | // Set the letter that creates your snowflake (recommended:*)
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| - | var snowletter="*"
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| - |
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| - | // Set the speed of sinking (recommended values range from 0.3 to 2)
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| - | var sinkspeed=0.6
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| - |
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| - | // Set the maximal-size of your snowflaxes
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| - | var snowmaxsize=22
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| - |
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| - | // Set the minimal-size of your snowflaxes
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| - | var snowminsize=8
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| - |
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| - | // Set the snowing-zone
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| - | // Set 1 for all-over-snowing, set 2 for left-side-snowing
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| - | // Set 3 for center-snowing, set 4 for right-side-snowing
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| - | var snowingzone=4
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| - |
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| - | ///////////////////////////////////////////////////////////////////////////
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| - | // CONFIGURATION ENDS HERE
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| - | ///////////////////////////////////////////////////////////////////////////
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| - |
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| - |
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| - | // Do not edit below this line
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| - | var snow=new Array()
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| - | var marginbottom
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| - | var marginright
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| - | var timer
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| - | var i_snow=0
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| - | var x_mv=new Array();
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| - | var crds=new Array();
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| - | var lftrght=new Array();
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| - | var browserinfos=navigator.userAgent
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| - | var ie5=document.all&&document.getElementById&&!browserinfos.match(/Opera/)
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| - | var ns6=document.getElementById&&!document.all
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| - | var opera=browserinfos.match(/Opera/)
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| - | var browserok=ie5||ns6||opera
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| - |
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| - | function randommaker(range) {
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| - | rand=Math.floor(range*Math.random())
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| - | return rand
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| - | }
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| - |
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| - | function initsnow() {
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| - | if (ie5 || opera) {
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| - | marginbottom = document.body.clientHeight
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| - | marginright = document.body.clientWidth
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| - | }
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| - | else if (ns6) {
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| - | marginbottom = window.innerHeight
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| - | marginright = window.innerWidth
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| - | }
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| - | var snowsizerange=snowmaxsize-snowminsize
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| - | for (i=0;i<=snowmax;i++) {
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| - | crds[i] = 0;
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| - | lftrght[i] = Math.random()*15;
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| - | x_mv[i] = 0.03 + Math.random()/10;
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| - | snow[i]=document.getElementById("s"+i)
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| - | snow[i].style.fontFamily=snowtype[randommaker(snowtype.length)]
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| - | snow[i].size=randommaker(snowsizerange)+snowminsize
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| - | snow[i].style.fontSize=snow[i].size
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| - | snow[i].style.color=snowcolor[randommaker(snowcolor.length)]
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| - | snow[i].sink=sinkspeed*snow[i].size/5
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| - | if (snowingzone==1) {snow[i].posx=randommaker(marginright-snow[i].size)}
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| - | if (snowingzone==2) {snow[i].posx=randommaker(marginright/2-snow[i].size)}
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| - | if (snowingzone==3) {snow[i].posx=randommaker(marginright/2-snow[i].size)+marginright/4}
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| - | if (snowingzone==4) {snow[i].posx=randommaker(marginright/2-snow[i].size)+marginright/2}
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| - | snow[i].posy=randommaker(6*marginbottom-marginbottom-6*snow[i].size)
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| - | snow[i].style.left=snow[i].posx
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| - | snow[i].style.top=snow[i].posy
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| - | }
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| - | movesnow()
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| - | }
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| - |
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| - | function movesnow() {
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| - | for (i=0;i<=snowmax;i++) {
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| - | crds[i] += x_mv[i];
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| - | snow[i].posy+=snow[i].sink
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| - | snow[i].style.left=snow[i].posx+lftrght[i]*Math.sin(crds[i]);
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| - | snow[i].style.top=snow[i].posy
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| - |
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| - | if (snow[i].posy>=marginbottom-6*snow[i].size || parseInt(snow[i].style.left)>(marginright-3*lftrght[i])){
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| - | if (snowingzone==1) {snow[i].posx=randommaker(marginright-snow[i].size)}
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| - | if (snowingzone==2) {snow[i].posx=randommaker(marginright/2-snow[i].size)}
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| - | if (snowingzone==3) {snow[i].posx=randommaker(marginright/2-snow[i].size)+marginright/4}
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| - | if (snowingzone==4) {snow[i].posx=randommaker(marginright/2-snow[i].size)+marginright/2}
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| - | snow[i].posy=0
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| - | }
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| - | }
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| - | var timer=setTimeout("movesnow()",50)
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| - | }
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| - |
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| - | for (i=0;i<=snowmax;i++) {
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| - | document.write("<span id='s"+i+"' style='position:absolute;top:-"+snowmaxsize+"'>"+snowletter+"</span>")
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| - | }
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| - | if (browserok) {
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| - | window.onload=initsnow
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| - | }
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| - | </script>
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| | <style type="text/css"> | | <style type="text/css"> |
| | | | |
Project Description:
Background:
Bacterial Microcompartments (MCP) are closed polyhedral shells, diameter of 100-150nm, which is made of thin protein sheets, enclosing enzymes and cofactors for various forms of fermentative metabolism. Salmonella enteric enthanolamine ultilization (Eut) MCP is one example.
5 genes (Eut S, M, N, L, K) encodes thousands of copies of shell proteins to form a heterogenous MCP shell and empty recombinant Eut Shell has been successfully expressed in E.coli.
In addition, a localization signal (N terminal of EutC19) has been identified and signal fused enzymes/ proteins could be targeted to the cavity of the MCP.
Inspired by these studies, we think this Eut MCP can become a versatile tool if we can:
(a) Modify its exterior surface to enable surface display
(b) Localize special enzyme into the MCP to enable specific metabolism
(c) Store useful or harmful molecules into the MCP
In this iGEM project, using Eut Microcompartment, we aim to show:
(1) MCP shell surface can be used to display specific peptides, without disrupting the MCP structure, to enable cell-specific targeting for drug delivery system, easy purification etc. We will display Flag and His tag as a proof of concept.
(2) Localize polyphosphate kinase (ppk1) into the native/ engineered MCP to polymerize inorganic phosphate and accumulate them in MCP, trying to treat phosphate pollution in waste water.
We will divide into 2 parallel sub-teams, specifically working on surface decoration and ppk enzyme.
"
