Project Description

Haloalkanes are widely used commercially. The majorty of these compounds have been shown to be serious pollutants as they are toxic and quite persistent in the environment, such as a man-made industrial chemical 1,2,3-Trichloropropane (TCP) and an organic pesticideγ-Hexachlorocyclohexane (Lindane，γ-HCH). These halogenated compounds have been introduced into our environment as a consequence of industrial waste disposal and widespread open use in agriculture, which need to be removed to low levels from waste streams and during sanitation of polluted sites. For this reason, the biodegradation of halogenated compounds is of great importance. In the project, several key enzymes in the biodegradation pathways of the two compounds were co-expressed in E. coli. As putting the different selected enzymes together, it will have a ability to degrade more halogenated compounds besides the both. To construct the efficient co-expression system and achieve biodegradation of γ-HCH and TCP, we adopt 2A peptide and polycistronic co-expression. Foot and mouth disease virus 2A peptide has been widely used for co-expression of multiple genes in eukaryote systems. However, the use of the 2A peptide in prokaryotes is limited, and so far only one paper described that F2A can work in E. coli as well. To explore whether the 2A peptides can work in our co-expression system, several vectors were constructed by using all three 2A peptides, respectively.

Animated Portfolio Gallery

Today we will create an animated portfolio gallery with jQuery. The gallery will contain a scroller for thumbnails and a content area where we will display details about the portfolio item. The image can be enlarged by clicking on it, making it appear as an overlay.

Annotation Overlay Effect

Today we will create a simple overlay effect to display annotations in e.g. portfolio items of a web designers portfolio. We got the idea from the wonderful portfolio of www.rareview.com where Flash is used to create the effect. We will use jQuery.

Bubbleriffic Image Gallery

In this tutorial we will create a bubbly image gallery that shows your images in a unique way. The idea is to show the thumbnails of albums in a rounded fashion allowing the user to scroll them automatically by moving the mouse. Clicking on a thumbnail will zoom in a big circle and the full image which will be automatically resized to fit into the screen.

Collapsing Site Navigation

Today we will create a collapsing menu that contains vertical navigation bars and a slide out content area. When hovering over a menu item, an image slides down from the top and a submenu slides up from the bottom. Clicking on one of the submenu items will make the whole menu collapse like a card deck and the respective content area will slide out.

Custom Animation Banner

In todayos tutorial we will be creating a custom animation banner with jQuery. The idea is to have different elements in a banner that will animate step-wise in a custom way.

We will be using the jQuery Easing Plugin and the jQuery 2D Transform Plugin to create some nifty animations.

Full Page Image Gallery

In this tutorial we are going to create a stunning full page gallery with scrollable thumbnails and a scrollable full screen preview. The idea is to have a thumbnails bar at the bottom of the page that scrolls automatically when the user moves the mouse. When a thumbnail is clicked, it moves to the center of the page and the full screen image is loaded in the background.

Hover Slide Effect

Today we will create a neat effect with some images using jQuery. The main idea is to have an image area with several images that slide out when we hover over them, revealing other images. The sliding effect will be random, i.e. the images will slide to the top or bottom, left or right, fading out or not. When we click on any area, all areas will slide their images out.

Merging Image Boxes

Today we will show you a nice effect for images with jQuery. The idea is to have a set of rotated thumbnails that, once clicked, animate to form the selected image. You can navigate through the images with previous and next buttons and when the big image gets clicked it will scatter into the little box shaped thumbnails again.

Compact News Previewer

Today we will create a news previewer that letos you show your latest articles or news in a compact way. The news previewer will show some list of articles on the left side and the preview of the article with a longer description on the right. Once a news on the left is clicked, the preview will slide in.

Overlay Effect Menu

In this tutorial we are going to create a simple menu that will stand out once we hover over it by covering everything except the menu with a dark overlay. The menu will stay white and a submenu area will expand. We will create this effect using jQuery.

Polaroid Photobar Gallery

In this tutorial we are going to create an image gallery with a Polaroid look. We will have albums that will expand to sets of slightly rotated thumbnails that pop out on hover. The full image will slide in from the bottom once a thumbnail is clicked. In the full image view the user can navigate through the pictures or simply choose another thumbnail to be displayed.

Pull Out Content Panel

In this tutorial we will create a content panel that slides out at a predefined scroll position. It will reveal a teaser with related content and it can be expanded to full page size to show more. A custom slider allows to scroll through many items in the panel.

Thumbnails Navigation Gallery

In this tutorial we are going to create an extraordinary gallery with scrollable thumbnails that slide out from a navigation. We are going to use jQuery and some CSS3 properties for the style. The main idea is to have a menu of albums where each item will reveal a horizontal bar with thumbnails when clicked.