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Team:UNITN-Trento/Notebook/Labposts/08/36
From 2013.igem.org
(Created page with "{ "date" : "2013-08-13", "author" : "fabio", "title" : " blue light induction", "content" : "<html> today I made some dilutions 1:100 from the inocula, to have 3 cultures (20...") |
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"title" : " blue light induction", | "title" : " blue light induction", | ||
"content" : "<html> today I made some dilutions 1:100 from the inocula, to have 3 cultures (200 ml) for the experiment: one will be induced with blue light, the other two are control sample, the fors exposed to standard light, the last one will stay “alone in the dark”. I started the experiment when the dilution cultures reached 0.7 OD. After 4 ours from the beginning of the induction I took 10 ml from the samples and obtained pellet to see blue pigment production: no difference. The experiment will go on over the night, tomorrow we will hopefully be able to see some difference. Meanwhile I made some inocula from the same plate, in order to repeat the experiment with less volume cultures.</html>", | "content" : "<html> today I made some dilutions 1:100 from the inocula, to have 3 cultures (200 ml) for the experiment: one will be induced with blue light, the other two are control sample, the fors exposed to standard light, the last one will stay “alone in the dark”. I started the experiment when the dilution cultures reached 0.7 OD. After 4 ours from the beginning of the induction I took 10 ml from the samples and obtained pellet to see blue pigment production: no difference. The experiment will go on over the night, tomorrow we will hopefully be able to see some difference. Meanwhile I made some inocula from the same plate, in order to repeat the experiment with less volume cultures.</html>", | ||
| - | "tags" : " | + | "tags" : " BlueLight_amilCP" |
} | } | ||
Revision as of 08:18, 30 August 2013
{ "date" : "2013-08-13", "author" : "fabio", "title" : " blue light induction", "content" : " today I made some dilutions 1:100 from the inocula, to have 3 cultures (200 ml) for the experiment: one will be induced with blue light, the other two are control sample, the fors exposed to standard light, the last one will stay “alone in the dark”. I started the experiment when the dilution cultures reached 0.7 OD. After 4 ours from the beginning of the induction I took 10 ml from the samples and obtained pellet to see blue pigment production: no difference. The experiment will go on over the night, tomorrow we will hopefully be able to see some difference. Meanwhile I made some inocula from the same plate, in order to repeat the experiment with less volume cultures.", "tags" : " BlueLight_amilCP" }
