Team:Yale/Project MAGE

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Revision as of 04:45, 31 August 2013

	Project Overview	Validate PLA synthesis	Develop bioassay	Apply MAGE	Introduce export system	Make a bioplastic

Aims for the Project

Engineer strains of E. coli to validate PLA synthesis
Develop bioassay to screen PLA production
Apply MAGE to optimize PLA production, guided by FBA
Introduce type 1 secretion system to export and extract PLA
Make a bioplastic

MAGE Targets

The first step in applying MAGE is finding MAGE targets. This involved reading numerous scientific papers learning as much as possible about the heterologous enzymes, and the pathway that was being used to create the PLA

List of Papers:
Jacob et al. 1997
Matsuzaki et al. 1998
Sawers et al. 1998
Park et al. 2002
Selmer et al. 2002
Takase et al. 2002
Fong et al. 2005
Matsumoto et al. 2005
Rangarajan ES et al. 2005
Matsumoto et al. 2006
Jung et al. 2009
Matsumoto et al. 2009
Juang et al. 2010
Orth et al. 2010
Yang et al. 2011
Kandasamy et al. 2012
Yang et al. 2013

@@ Line 21: / Line 21: @@
 <br>
-=== Apply MAGE to optimize PLA production, guided by FBA ===
+== MAGE Targets ==
+*The first step in applying MAGE is finding MAGE targets.  This involved reading numerous scientific papers learning as much as possible about the heterologous enzymes, and the pathway that was being used to create the PLA
+[[File:Metabolic Engineering Graphic.png|800px]]
+List of Papers:<br>
+Jacob et al. 1997 <br>
+Matsuzaki et al. 1998<br>
+Sawers et al. 1998<br>
+Park et al. 2002<br>
+Selmer et al. 2002<br>
+Takase et al. 2002<br>
+Fong et al. 2005<br>
+Matsumoto et al. 2005<br>
+Rangarajan ES et al. 2005<br>
+Matsumoto et al. 2006<br>
+Jung et al. 2009<br>
+Matsumoto et al. 2009<br>
+Juang et al. 2010<br>
+Orth et al. 2010<br>
+Yang et al. 2011<br>
+Kandasamy et al. 2012<br>
+Yang et al. 2013<br>