Team:Washington/Protocols

From 2013.igem.org

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<a href="https://2013.igem.org/Team:Washington"><img class="thumbnail"  src='https://static.igem.org/mediawiki/2013/1/1b/Team_Washington2013_banner_final.jpg' width= "965"/></A>
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<a href="https://2012.igem.org/Main_Page"><img src='https://static.igem.org/mediawiki/igem.org/c/c8/IGEM_desat.png' align="right" width= "70"/></a>
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<nav id="igemuwmenu-wrap">   
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<ul id="igemuwmenu">
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-
<li><a href="https://2013.igem.org/Team:Washington">Home</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:Washington">UW 2013</a></li>
+
-
<li><a href="https://2012.igem.org/Team:Washington">UW 2012</a></li>
+
-
<li><a href="https://2011.igem.org/Team:Washington">UW 2011</a></li>
+
-
<li><a href="https://2010.igem.org/Team:Washington">UW 2010</a></li>
+
-
<li><a href="https://2009.igem.org/Team:Washington">UW 2009</a></li>
+
-
<li><a href="https://2008.igem.org/Team:University_of_Washington">UW 2008</a></li>
+
-
<li><a href="https://2013.igem.org/Main_Page">iGEM Homepage</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
<!--
+
-
<li>
+
-
<a </a> main menu on plastic degradation
+
-
<ul>
+
-
 
+
-
for the drop down of plastic degradation
+
-
format <li><a href="https://2012.igem.org/Team:Washington/Plastics#Background">Background</a></li>
+
-
 
+
-
 
+
-
</ul>
+
-
</li>
+
-
-->
+
-
 
+
-
 
+
-
<li>
+
-
<a href="https://2013.igem.org/Team:Washington/LightSensing">Light Sensing Experiments</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Background">Background</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Cloning">Cloning</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Systems">Our Systems</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Methods">Methods</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Results">Results</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Sources">Scientific Sources</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Future">Future Plans</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Parts">Parts Submitted</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
 
+
-
<li>
+
-
<a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE">e.colightTune</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Background">Background</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Usage">App</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Benefits">Methods</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Results">Results Summary</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
<li>
+
-
<a href="https://2013.igem.org/Team:Washington/Outreach">Outreach</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:Washington/Outreach#Activities">Activities</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/Outreach#Pictures">Pictures</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
<li>
+
-
<a href="https://2013.igem.org/Team:Washington/Protocols">Protocols</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:Washington/Protocols#General_Protocols">General</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/Protocols#Cloning_Protocols">Cloning</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/Protocols#Assay">Light assays</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
<li>
+
-
<a href="https://2013.igem.org/Team:Washington/MoreLinks">Links</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:Washington/MoreLinks#Safety">Safety Form</a></li>
+
-
<li><a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=Washington">Parts Registry</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Washington/MoreLinks#Documentation">Parts Documentation</a></li>
+
-
 
+
-
</ul>
+
-
</li>
+
-
 
+
-
<li style="border-right:0; box-shadow:0px 0 0;">
+
-
<a href="https://2013.igem.org/Team:Washington/Funding">Funding</a>
+
-
</li>
+
-
 
+
-
</li>
+
-
                                                                                               
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</ul>
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+
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/* Mobile */
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</html>
 
-
==''''''==
 
<html>
<html>
-
 
+
<blockquote>
<header>
<header>
-
<b><u>Cloning Protocols Workflow</u></b>
+
<b><u>Cloning Protocols</u></b>
</header>
</header>
-
 
-
<br>
 
<body>
<body>
<p>
<p>
-
<b>Tips and Etiquette:</b>
 
<ul>
<ul>
-
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">General Basic Protocol Information</a></li>
+
<li><a href = "https://2013.igem.org/Team:Washington/iGEMVectors">iGEM Vector Information</a></li>
-
<li><a href = "https://2013.igem.org/Team:Washington/HOW_TO_LABEL_EVERYTHING">How to Label EVERYTHING</a></li>
+
<li><a href = "https://2013.igem.org/Team:Washington/General_Cloning_Strategy">General Cloning Strategy</a></li>
-
&nbsp;<li>Keep notebooks up-to-date</li>
+
</ul>
</ul>
-
</p>
+
<b>Workflow:</b>
-
<br>
 
-
 
-
<p>
 
-
<b>Workflow:</b>
 
<ul>
<ul>
-
<li><a href = "https://2013.igem.org/Team:Washington/PRIMER_DESIGN">Primer Design</a></li>
 
-
<li><a href = "https://2013.igem.org/Team:Washington/STREAKING_A_PLATE">Streaking a Plate for Isolation</a></li>
 
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<li><a href = "https://2013.igem.org/Team:Washington/OVERNIGHT_CULTURES">Overnight Cultures</a></li>
 
<li><a href = "https://2013.igem.org/Team:Washington/ISOLATION_OF_PLASMID">1) Isolation of Plasmid DNA (miniprep)</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/ISOLATION_OF_PLASMID">1) Isolation of Plasmid DNA (miniprep)</a></li>
-
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_PCR_PROTOCOL">2) General PCR Protocol </a>(also see <a href = "https://2013.igem.org/Team:Washington/PCR_GOTAG">PCR GoTag</a> - product (30-200ng/ ul) Check on gel PCR Purification and/or  <a href = "https://2013.igem.org/Team:Washington/DPNL_DIGEST">Dpnl Digest</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_PCR_PROTOCOL">2) General PCR Protocol </a>(also see <a href = "https://2013.igem.org/Team:Washington/PCR_GOTAG">PCR GoTaq</a> - product (30-200ng/ ul) Check on gel PCR Purification and/or  <a href = "https://2013.igem.org/Team:Washington/DPNL_DIGEST">Dpnl Digest</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_DIGESTION_PROTOCOL">3) General Digestion Protocol</a> Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)</li>
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_DIGESTION_PROTOCOL">3) General Digestion Protocol</a> Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)</li>
<li><a href = "https://2013.igem.org/Team:Washington/AGAROSE_GEL">4) Agarose Gel Electrophoresis</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/AGAROSE_GEL">4) Agarose Gel Electrophoresis</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/COLONY_PCR_GREEN">7) Colony PCR with Green taq</a> Miniprep (stocks can be made from this culture - add 1ml extra)</li>
<li><a href = "https://2013.igem.org/Team:Washington/COLONY_PCR_GREEN">7) Colony PCR with Green taq</a> Miniprep (stocks can be made from this culture - add 1ml extra)</li>
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<li><a href = "https://2013.igem.org/Team:Washington/DNA_SEQUENCING">8) DNA Sequencing</a></li>
 
<li><a href = "https://2013.igem.org/Team:Washington/MAKING_GLYCEROL_FROZEN_STOCKS">9) Making Glycerol Frozen Stocks</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/MAKING_GLYCEROL_FROZEN_STOCKS">9) Making Glycerol Frozen Stocks</a></li>
</ul>
</ul>
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<br>
<br>
-
<p>
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<b><u>Light Sensing Protocols</u></b>
-
<b>Media Preparation:</b>
+
-
<ul>
+
-
<li><a href = "https://2013.igem.org/Team:Washington/COMPETENT_CELL_STOCKS">Competent Cell Stocks</a></li>
+
-
<li><a href = "https://2013.igem.org/Team:Washington/LB_AND_TB_MEDIA">LB and TB Media</a></li>
+
-
<li><a href = "https://2013.igem.org/Team:Washington/POURING_AGAR_PLATES">Pouring Agar Plates</a></li>
+
-
</ul>
+
-
</p>
+
-
 
+
<br>
<br>
<p>
<p>
-
<b>Instruments:</b>
+
<b>Light Sensing Media:</b>
-
<ul>
+
<ul><li><a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></li>
-
<li><a href = "https://2013.igem.org/Team:Washington/NANODROP_PROTOCOL">Nanodrop Protocol</a></li>
+
<li><a href = "https://2013.igem.org/Team:Washington/M9Media">M9 Media + Casamino Acid Recipe</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/M9Plate">M9 + Casamino Acid Plate</a></li></ul>
 +
<b>Light Testing:</b><ul>
 +
<li><a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">General Protocol</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate Assay</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/60mmPetriDishAssay">60 mm Petri Dish Assay</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/LightIntensityTest">Light Intensity Test</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/BlinkingTest">Blinking Test</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/GFPExpression">GFP Expression on Agar Plates</a></li>
</ul>
</ul>
-
</p>
+
<b>Setting Up The App:</b><ul>
-
 
+
<li><a href = "https://2013.igem.org/Team:Washington/appsetup">E.colight Setup</a></li></ul>
-
<br>
+
<b>App Testing:</b><ul>
-
 
+
<li><a href = "https://2013.igem.org/Team:Washington/BleedThroughTest">Bleed-Through Test</a></li>
-
<p>
+
-
<b>See also:</b>
+
-
<ul>
+
-
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BIOTECHNOLOGY_INFORMATION">General Biotechnology Information</a></li>
+
-
<li><a href = "https://2013.igem.org/Team:Washington/SOS_LAB_PROTOCOLS">SOS Lab Protocols</a></li>
+
-
<li><a href = "http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf">http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf</a></li>
+
</ul>
</ul>
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</p>
 
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</body>
</body>
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<br>
</html>
</html>

Latest revision as of 21:55, 27 September 2013



Cloning Protocols

Workflow:


Light Sensing Protocols

Light Sensing Media:

Light Testing: Setting Up The App: App Testing:

Retrieved from "http://2013.igem.org/Team:Washington/Protocols"