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| - | <html>
| + | {{:Team:Washington/WA_Template}} |
| | | | |
| - | <style type="text/css">
| |
| - | .firstHeading { /* Gets rid of the default iGEM picture*/
| |
| - | height:2px;
| |
| - | visibility:hidden;
| |
| - | }
| |
| - | #catlinks {
| |
| - | display: none;
| |
| - | }
| |
| - | #content {
| |
| - | border-left-width:0px;
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| - | border-right-width:0px;
| |
| - | padding: 0px 5px 0px 5px;
| |
| - | width:965px; /* Content width*/
| |
| - | }
| |
| - | #menubar {
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| - | background-color: #FFFFFF; /* left menu bar color*/
| |
| - | }
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| - | #menubar ul li a {
| |
| - | color: #545353; } /* menu text color*/
| |
| - | .right-menu li a {
| |
| - | color: red;
| |
| - | background-color: #FFFFFF; /* right menu bar color*/
| |
| - | }
| |
| - | #p-logo { height 1px; overflow:hidden; display: none;
| |
| - | }
| |
| - | #top-section {
| |
| - | background-position: center center;
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| - | background-repeat: no-repeat;
| |
| - | background-color: #FFFFFF; /* top header background color*/
| |
| - | border-width:0px;
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| - | height:10px; /* top header height (determines how low content will start to appear)*/
| |
| - | }
| |
| - | #siteSub {
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| - | display:none;
| |
| - | }
| |
| - | #search-controls { /* gets rid of the search bar (it was ugly) */
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| - | display:none;
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| - | margin-top:0px;
| |
| - | }
| |
| - | #contentSub {
| |
| - | display:none;
| |
| - | }
| |
| | | | |
| - | /*Now to set heading and body fonts*/
| |
| - | body {
| |
| - | background:#000000;
| |
| - | background-image: url("https://static.igem.org/mediawiki/2012/1/1b/Gradientback.jpg");
| |
| - | background-position: center;
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| - | background-repeat: repeat-y;
| |
| - | }
| |
| - | h1
| |
| - | {
| |
| - | color:#262626;
| |
| - | text-align:left;
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| - | font-family:"Trebuchet";
| |
| - | font-size:25px;
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| - | line-height:100%;
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| - | border-bottom: none;
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| - | padding:0px 0px 0px 0px;
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| - | margin: 0px 0px 0px 0px;
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| - | }
| |
| - | h2
| |
| - | {
| |
| - | color:#262626;
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| - | text-align:left;
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| - | font-family:"Trebuchet";
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| - | font-size:20px;
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| - | line-height: 100%;
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| - | border-bottom: none;
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| - | padding:0px 0px 0px 0px;
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| - | margin: 0px 0px 0px 0x;
| |
| - | }
| |
| - | h3
| |
| - | {
| |
| - | color:#262626;
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| - | text-align:left;
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| - | font-family:"Trebuchet";
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| - | font-size:17px;
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| - | line-height: 100%;
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| - | border-bottom: none;
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| - | padding:0px 0px 0px 0px;
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| - | margin: 0px 0px 0px 0x;
| |
| - | }
| |
| - | p
| |
| - | {
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| - | color:#262626;
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| - | text-align: justify;
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| - | font-family:"Arial";
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| - | font-size:14px;
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| - | line-height:100%
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| - | border-bottom: none;
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| - | padding:0px 0px 0px 0px;
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| - | margin: 0px 0px 0px 0x;
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| - | border:0px
| |
| - | }
| |
| - | body {
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| - | color:#808080;
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| - | line-height: 1;
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| - | font-family:"Arial";
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| - | font-size:11px;
| |
| - | }
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| - |
| |
| - | .whitebox {
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| - | -moz-box-shadow: 0px 0px 0px #ccc;
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| - | -webkit-box-shadow: 0px 0px 0px #ccc;
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| - | box-shadow: 0px 0px 15px #ccc;
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| - | background-color: #FFFFFF;
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| - | -moz-border-radius: 1px;
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| - | border-radius: 15px;
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| - | margin-top: 20px;
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| - | padding-top: 20px;
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| - | padding-left: 20px;
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| - | padding-right: 20px;
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| - | padding-bottom: 20px;
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| - | margin-left: 15px;
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| - | margin-right: 25px;
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| - | }
| |
| - | .thumbnail
| |
| - | {
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| - | margin-bottom:0px;
| |
| - | padding-bottom:0px;
| |
| - | position:relative;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu, #igemuwmenu ul {
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| - | margin: 0;
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| - | padding: 0;
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| - | list-style: none;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu {
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| - | width: 820px;
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| - | margin: 10px auto;
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| - | border: 1px solid #222;
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| - | background-color: #111;
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| - | background-image: -moz-linear-gradient(#444, #111);
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| - | background-image: -webkit-gradient(linear, left top, left bottom, from(#444), to(#111));
| |
| - | background-image: -webkit-linear-gradient(#444, #111);
| |
| - | background-image: -o-linear-gradient(#444, #111);
| |
| - | background-image: -ms-linear-gradient(#444, #111);
| |
| - | background-image: linear-gradient(#444, #111);
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| - | -moz-border-radius: 6px;
| |
| - | -webkit-border-radius: 6px;
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| - | border-radius: 6px;
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| - | -moz-box-shadow: 0 1px 1px #777, 0 1px 0 #666 inset;
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| - | -webkit-box-shadow: 0 1px 1px #777, 0 1px 0 #666 inset;
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| - | box-shadow: 0 1px 1px #777, 0 1px 0 #666 inset;
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| - | display: inline-block;
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| - | white-space: nowrap;
| |
| - | }
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| - |
| |
| - | #igemuwmenu:before,
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| - | #igemuwmenu:after {
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| - | content: "";
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| - | display: table;
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| - | }
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| - |
| |
| - | #igemuwmenu:after {
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| - | clear: both;
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| - | }
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| - |
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| - | #igemuwmenu {
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| - | zoom:1;
| |
| - | }
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| - |
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| - | #igemuwmenu li {
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| - | border-right: 1px solid #222;
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| - | -moz-box-shadow: 1px 0 0 #444;
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| - | -webkit-box-shadow: 1px 0 0 #444;
| |
| - | box-shadow: 1px 0 0 #444;
| |
| - | position: relative;
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| - | display: inline-block;
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| - | white-space: nowrap;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu a {
| |
| - | padding: 12px 20px;
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| - | color: #999;
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| - | text-transform: uppercase;
| |
| - | font: bold 11.4px Arial, Helvetica;
| |
| - | text-decoration: none;
| |
| - | text-shadow: 0 1px 0 #000;
| |
| - | display: inline-block;
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| - | white-block: nowrap;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu li:hover > a {
| |
| - | color: #fafafa;
| |
| - | }
| |
| - |
| |
| - | *html #igemuwmenu li a:hover { /* IE6 only */
| |
| - | color: #fafafa;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul {
| |
| - | margin: 20px 0 0 0;
| |
| - | _margin: 0; /*IE6 only*/
| |
| - | opacity: 0;
| |
| - | visibility: hidden;
| |
| - | position: absolute;
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| - | top: 38px;
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| - | left: 0;
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| - | z-index: 1;
| |
| - | background: #444;
| |
| - | background: -moz-linear-gradient(#444, #111);
| |
| - | background-image: -webkit-gradient(linear, left top, left bottom, from(#444), to(#111));
| |
| - | background: -webkit-linear-gradient(#444, #111);
| |
| - | background: -o-linear-gradient(#444, #111);
| |
| - | background: -ms-linear-gradient(#444, #111);
| |
| - | background: linear-gradient(#444, #111);
| |
| - | -moz-box-shadow: 0 -1px rgba(255,255,255,.3);
| |
| - | -webkit-box-shadow: 0 -1px 0 rgba(255,255,255,.3);
| |
| - | box-shadow: 0 -1px 0 rgba(255,255,255,.3);
| |
| - | -moz-border-radius: 3px;
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| - | -webkit-border-radius: 3px;
| |
| - | border-radius: 3px;
| |
| - | -webkit-transition: all .2s ease-in-out;
| |
| - | -moz-transition: all .2s ease-in-out;
| |
| - | -ms-transition: all .2s ease-in-out;
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| - | -o-transition: all .2s ease-in-out;
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| - | transition: all .2s ease-in-out;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu li:hover > ul {
| |
| - | opacity: 1;
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| - | visibility: visible;
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| - | margin: 0;
| |
| - | }
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| - |
| |
| - | #igemuwmenu ul ul {
| |
| - | top: 0;
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| - | left: 150px;
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| - | margin: 0 0 0 20px;
| |
| - | _margin: 0; /*IE6 only*/
| |
| - | -moz-box-shadow: -1px 0 0 rgba(255,255,255,.3);
| |
| - | -webkit-box-shadow: -1px 0 0 rgba(255,255,255,.3);
| |
| - | box-shadow: -1px 0 0 rgba(255,255,255,.3);
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul li {
| |
| - | float: none;
| |
| - | display: block;
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| - | border: 0;
| |
| - | _line-height: 0; /*IE6 only*/
| |
| - | -moz-box-shadow: 0 1px 0 #111, 0 2px 0 #666;
| |
| - | -webkit-box-shadow: 0 1px 0 #111, 0 2px 0 #666;
| |
| - | box-shadow: 0 1px 0 #111, 0 2px 0 #666;
| |
| - | }
| |
| - | #igemuwmenu ul li.active {
| |
| - | float: none;
| |
| - | display: block;
| |
| - | border: 0;
| |
| - | _line-height: 0; /*IE6 only*/
| |
| - | -moz-box-shadow: 0 1px 0 #111, 0 2px 0 #666;
| |
| - | -webkit-box-shadow: 0 1px 0 #111, 0 2px 0 #666;
| |
| - | box-shadow: 0 1px 0 #111, 0 2px 0 #666;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | #igemuwmenu ul li:last-child {
| |
| - | -moz-box-shadow: none;
| |
| - | -webkit-box-shadow: none;
| |
| - | box-shadow: none;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul a {
| |
| - | padding: 10px;
| |
| - | width: 130px;
| |
| - | _height: 10px; /*IE6 only*/
| |
| - | display: block;
| |
| - | white-space: nowrap;
| |
| - | float: none;
| |
| - | text-transform: none;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul a.selected {
| |
| - | padding: 10px;
| |
| - | width: 130px;
| |
| - | _height: 10px; /*IE6 only*/
| |
| - | display: block;
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| - | white-space: nowrap;
| |
| - | float: none;
| |
| - | text-transform: none;
| |
| - | }
| |
| - |
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| - | #igemuwmenu ul a:hover {
| |
| - | background-color: #785D84;
| |
| - | background-image: -moz-linear-gradient(#B6A0BA, #785D84);
| |
| - | background-image: -webkit-gradient(linear, left top, left bottom, from(#B6A0BA), to(#785D84));
| |
| - | background-image: -webkit-linear-gradient(#B6A0BA, #785D84);
| |
| - | background-image: -o-linear-gradient(#B6A0BA, #785D84);
| |
| - | background-image: -ms-linear-gradient(#B6A0BA, #785D84);
| |
| - | background-image: linear-gradient(#B6A0BA, #785D84);
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul li:first-child > a {
| |
| - | -moz-border-radius: 3px 3px 0 0;
| |
| - | -webkit-border-radius: 3px 3px 0 0;
| |
| - | border-radius: 3px 3px 0 0;
| |
| - | }
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| - |
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| - | #igemuwmenu ul li:first-child > a:after {
| |
| - | content: '';
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| - | position: absolute;
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| - | left: 40px;
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| - | top: -6px;
| |
| - | border-left: 6px solid transparent;
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| - | border-right: 6px solid transparent;
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| - | border-bottom: 6px solid #444;
| |
| - | }
| |
| - |
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| - | #igemuwmenu ul ul li:first-child a:after {
| |
| - | left: -6px;
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| - | top: 50%;
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| - | margin-top: -6px;
| |
| - | border-left: 0;
| |
| - | border-bottom: 6px solid transparent;
| |
| - | border-top: 6px solid transparent;
| |
| - | border-right: 6px solid #3b3b3b;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul li:first-child a:hover:after {
| |
| - | border-bottom-color: #B6A0BA;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul ul li:first-child a:hover:after {
| |
| - | border-right-color: #0299d3;
| |
| - | border-bottom-color: transparent;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu ul li:last-child > a {
| |
| - | -moz-border-radius: 0 0 3px 3px;
| |
| - | -webkit-border-radius: 0 0 3px 3px;
| |
| - | border-radius: 0 0 3px 3px;
| |
| - | }
| |
| - |
| |
| - | /* Mobile */
| |
| - | #igemuwmenu-trigger {
| |
| - | display: none;
| |
| - | }
| |
| - |
| |
| - | @media screen and (max-width: 600px) {
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| - |
| |
| - | /* nav-wrap */
| |
| - | #igemuwmenu-wrap {
| |
| - | position: relative;
| |
| - | }
| |
| - |
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| - | #igemuwmenu-wrap * {
| |
| - | -moz-box-sizing: border-box;
| |
| - | -webkit-box-sizing: border-box;
| |
| - | box-sizing: border-box;
| |
| - | }
| |
| - |
| |
| - | /* menu icon */
| |
| - | #igemuwmenu-trigger {
| |
| - | display: block; /* show menu icon */
| |
| - | height: 40px;
| |
| - | line-height: 40px;
| |
| - | cursor: pointer;
| |
| - | padding: 0 0 0 35px;
| |
| - | border: 1px solid #222;
| |
| - | color: #fafafa;
| |
| - | font-weight: bold;
| |
| - | background-color: #111;
| |
| - | background: url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAABIAAAAPCAMAAADeWG8gAAAAGXRFWHRTb2Z0d2FyZQBBZG9iZSBJbWFnZVJlYWR5ccllPAAAAyBpVFh0WE1MOmNvbS5hZG9iZS54bXAAAAAAADw/eHBhY2tldCBiZWdpbj0i77u/IiBpZD0iVzVNME1wQ2VoaUh6cmVTek5UY3prYzlkIj8+IDx4OnhtcG1ldGEgeG1sbnM6eD0iYWRvYmU6bnM6bWV0YS8iIHg6eG1wdGs9IkFkb2JlIFhNUCBDb3JlIDUuMC1jMDYwIDYxLjEzNDc3NywgMjAxMC8wMi8xMi0xNzozMjowMCAgICAgICAgIj4gPHJkZjpSREYgeG1sbnM6cmRmPSJodHRwOi8vd3d3LnczLm9yZy8xOTk5LzAyLzIyLXJkZi1zeW50YXgtbnMjIj4gPHJkZjpEZXNjcmlwdGlvbiByZGY6YWJvdXQ9IiIgeG1sbnM6eG1wPSJodHRwOi8vbnMuYWRvYmUuY29tL3hhcC8xLjAvIiB4bWxuczp4bXBNTT0iaHR0cDovL25zLmFkb2JlLmNvbS94YXAvMS4wL21tLyIgeG1sbnM6c3RSZWY9Imh0dHA6Ly9ucy5hZG9iZS5jb20veGFwLzEuMC9zVHlwZS9SZXNvdXJjZVJlZiMiIHhtcDpDcmVhdG9yVG9vbD0iQWRvYmUgUGhvdG9zaG9wIENTNSBXaW5kb3dzIiB4bXBNTTpJbnN0YW5jZUlEPSJ4bXAuaWlkOjE2QjAxNjRDOUNEOTExRTE4RTNFRkI1RDQ2MUYxOTQ3IiB4bXBNTTpEb2N1bWVudElEPSJ4bXAuZGlkOjE2QjAxNjREOUNEOTExRTE4RTNFRkI1RDQ2MUYxOTQ3Ij4gPHhtcE1NOkRlcml2ZWRGcm9tIHN0UmVmOmluc3RhbmNlSUQ9InhtcC5paWQ6MTZCMDE2NEE5Q0Q5MTFFMThFM0VGQjVENDYxRjE5NDciIHN0UmVmOmRvY3VtZW50SUQ9InhtcC5kaWQ6MTZCMDE2NEI5Q0Q5MTFFMThFM0VGQjVENDYxRjE5NDciLz4gPC9yZGY6RGVzY3JpcHRpb24+IDwvcmRmOlJERj4gPC94OnhtcG1ldGE+IDw/eHBhY2tldCBlbmQ9InIiPz42AEtnAAAABlBMVEX///////9VfPVsAAAAAnRSTlP/AOW3MEoAAAAWSURBVHjaYmAgFzBiACKFho6NAAEGAD07AG1pn932AAAAAElFTkSuQmCC) no-repeat 10px center, -moz-linear-gradient(#444, #111);
| |
| - | background: url(data:image/png;base64,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) no-repeat 10px center, -webkit-linear-gradient(#444, #111);
| |
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| |
| - | -moz-border-radius: 6px;
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| - | -webkit-border-radius: 6px;
| |
| - | border-radius: 6px;
| |
| - | -moz-box-shadow: 0 1px 1px #777, 0 1px 0 #666 inset;
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| - | -webkit-box-shadow: 0 1px 1px #777, 0 1px 0 #666 inset;
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| - | box-shadow: 0 1px 1px #777, 0 1px 0 #666 inset;
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| - | }
| |
| - |
| |
| - | /* main nav */
| |
| - | #igemuwmenu {
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| - | margin: 0; padding: 10px;
| |
| - | position: absolute;
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| - | top: 40px;
| |
| - | width: 100%;
| |
| - | z-index: 1;
| |
| - | background-color: #444;
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| - | display: none;
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| - | -moz-box-shadow: none;
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| - | -webkit-box-shadow: none;
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| - | box-shadow: none;
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| - | }
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| - |
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| - | #igemuwmenu:after {
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| - | content: '';
| |
| - | position: absolute;
| |
| - | left: 25px;
| |
| - | top: -8px;
| |
| - | border-left: 8px solid transparent;
| |
| - | border-right: 8px solid transparent;
| |
| - | border-bottom: 8px solid #444;
| |
| - | }
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| - |
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| - | #igemuwmenu ul {
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| - | position: static;
| |
| - | visibility: visible;
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| - | opacity: 1;
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| - | margin: 0;
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| - | background: none;
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| - | -moz-box-shadow: none;
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| - | -webkit-box-shadow: none;
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| - | box-shadow: none;
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| - | }
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| - |
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| - | #igemuwmenu ul ul {
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| - | margin: 0 0 0 20px !important;
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| - | -moz-box-shadow: none;
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| - | -webkit-box-shadow: none;
| |
| - | box-shadow: none;
| |
| - | }
| |
| - |
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| - | #igemuwmenu li {
| |
| - | position: static;
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| - | display: block;
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| - | float: none;
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| - | border: 0;
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| - | margin: 5px;
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| - | -moz-box-shadow: none;
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| - | -webkit-box-shadow: none;
| |
| - | box-shadow: none;
| |
| - | }
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| - |
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| - | #igemuwmenu ul li{
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| - | margin-left: 20px;
| |
| - | -moz-box-shadow: none;
| |
| - | -webkit-box-shadow: none;
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| - | box-shadow: none;
| |
| - | }
| |
| - |
| |
| - | #igemuwmenu a{
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| - | display: block;
| |
| - | float: none;
| |
| - | padding: 0;
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| - | color: #999;
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| - | }
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| - |
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| - | #igemuwmenu a:hover{
| |
| - | color: #fafafa;
| |
| - | }
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| - |
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| - | #igemuwmenu ul a{
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| - | padding: 0;
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| - | width: auto;
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| - | }
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| - |
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| - | #igemuwmenu ul a:hover{
| |
| - | background: none;
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| - | }
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| - |
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| - | #igemuwmenu ul li:first-child a:after,
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| - | #igemuwmenu ul ul li:first-child a:after {
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| - | border: 0;
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| - | }
| |
| - |
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| - | }
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| - |
| |
| - | @media screen and (min-width: 600px) {
| |
| - | #igemuwmenu {
| |
| - | display: block !important;
| |
| - | }
| |
| - | }
| |
| - |
| |
| - | /* iPad */
| |
| - | .no-transition {
| |
| - | -webkit-transition: none;
| |
| - | -moz-transition: none;
| |
| - | -ms-transition: none;
| |
| - | -o-transition: none;
| |
| - | transition: none;
| |
| - | opacity: 1;
| |
| - | visibility: visible;
| |
| - | display: none;
| |
| - | }
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| - |
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| - | #igemuwmenu li:hover > .no-transition {
| |
| - | display: block;
| |
| - | }
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <a href="https://2013.igem.org/Team:Washington"><img class="thumbnail" src='https://static.igem.org/mediawiki/2013/1/1b/Team_Washington2013_banner_final.jpg' width= "965"/></A>
| |
| - |
| |
| - | <a href="http://www.washington.edu/"><img src='https://static.igem.org/mediawiki/2012/2/25/UW_W-Logo_smallRGB.jpg' align="left" width= "57" style="padding-top: 12px; padding-left: 5px;"/></a>
| |
| - |
| |
| - | <a href="https://2012.igem.org/Main_Page"><img src='https://static.igem.org/mediawiki/igem.org/c/c8/IGEM_desat.png' align="right" width= "70"/></a>
| |
| - |
| |
| - | <style type="text/css">
| |
| - | #content {z-index:4;}
| |
| - | .expand {background: url('https://static.igem.org/mediawiki/2008/e/ef/Icon-expand.png') no-repeat 95% 50%;}
| |
| - | .collapse {background: url('https://static.igem.org/mediawiki/2008/c/cd/Icon-collapse.png') no-repeat 95% 50%;}
| |
| - | .docked {background: #99ccff url("https://static.igem.org/mediawiki/2008/6/62/Ddnavundock.png") no-repeat 50% 50%;}
| |
| - | .undocked {background: #99ccff url("https://static.igem.org/mediawiki/2008/e/e4/Ddnavdock.png") no-repeat 50% 50%;}
| |
| - | </style>
| |
| - |
| |
| - | <nav id="igemuwmenu-wrap">
| |
| - |
| |
| - | <ul id="igemuwmenu">
| |
| - |
| |
| - | <li><a href="https://2013.igem.org/Team:Washington">Home</a>
| |
| - | <ul>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington">UW 2013</a></li>
| |
| - | <li><a href="https://2012.igem.org/Team:Washington">UW 2012</a></li>
| |
| - | <li><a href="https://2011.igem.org/Team:Washington">UW 2011</a></li>
| |
| - | <li><a href="https://2010.igem.org/Team:Washington">UW 2010</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Washington">UW 2009</a></li>
| |
| - | <li><a href="https://2008.igem.org/Team:University_of_Washington">UW 2008</a></li>
| |
| - | <li><a href="https://2013.igem.org/Main_Page">iGEM Homepage</a></li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <!--
| |
| - | <li>
| |
| - | <a </a> main menu on plastic degradation
| |
| - | <ul>
| |
| - |
| |
| - | for the drop down of plastic degradation
| |
| - | format <li><a href="https://2012.igem.org/Team:Washington/Plastics#Background">Background</a></li>
| |
| - |
| |
| - |
| |
| - | </ul>
| |
| - | </li>
| |
| - | -->
| |
| - |
| |
| - |
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Washington/LightSensing">Light Sensing Experiments</a>
| |
| - | <ul>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Background">Background</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Cloning">Cloning</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Systems">Our Systems</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Methods">Methods</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Results">Results</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Sources">Scientific Sources</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Future">Future Plans</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/LightSensing#Parts">Parts Submitted</a></li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - |
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE">e.colightTune</a>
| |
| - | <ul>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Background">Background</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Usage">App</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Benefits">Methods</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Results">Results Summary</a></li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Washington/Outreach">Outreach</a>
| |
| - | <ul>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/Outreach#Activities">Activities</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/Outreach#Pictures">Pictures</a></li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Washington/Protocols">Protocols</a>
| |
| - | <ul>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/Protocols#General_Protocols">General</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/Protocols#Cloning_Protocols">Cloning</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/Protocols#Assay">Light assays</a></li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Washington/MoreLinks">Links</a>
| |
| - | <ul>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/MoreLinks#Safety">Safety Form</a></li>
| |
| - | <li><a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=Washington">Parts Registry</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Washington/MoreLinks#Documentation">Parts Documentation</a></li>
| |
| - |
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <li style="border-right:0; box-shadow:0px 0 0;">
| |
| - | <a href="https://2013.igem.org/Team:Washington/Funding">Funding</a>
| |
| - | </li>
| |
| - |
| |
| - | </li>
| |
| - |
| |
| - | </ul>
| |
| - | </nav>
| |
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| - | =="""==
| |
| | | | |
| | <html> | | <html> |
| | <body> | | <body> |
| - | Making Glycerol Stocks<br />
| + | <blockquote> |
| - | <a href="https://docs.google.com/document/d/19IXxggALb9FxxdzAu4593g3quBs1JZgo1vpj2-hjtNQ/edit?usp=drive_web">Streaking a Plate for Isolation</a><br />
| + | |
| - | Pick only a single well isolated colony for making glycerol stocks. <br />
| + | |
| - | <br />
| + | |
| - | <br />
| + | |
| | <strong>Summary:</strong> <br /> | | <strong>Summary:</strong> <br /> |
| | For the long-term storage of microbial cultures <br /> | | For the long-term storage of microbial cultures <br /> |
| Line 657: |
Line 11: |
| | 1. Grow overnight culture (1 mL) of your cells in selective media <br /> | | 1. Grow overnight culture (1 mL) of your cells in selective media <br /> |
| | 2. Mix 500 mL of your culture with 500 mL of 40-60% glycerol in a cryo tube (or 1.5mL tube). Immediately place at <strong>-80 C</strong> (high concentration of glycerol is toxic to cells and prolonged exposure to it kills them at RT). <br /> | | 2. Mix 500 mL of your culture with 500 mL of 40-60% glycerol in a cryo tube (or 1.5mL tube). Immediately place at <strong>-80 C</strong> (high concentration of glycerol is toxic to cells and prolonged exposure to it kills them at RT). <br /> |
| - | 3. LABEL VERY WELL and add information to drive. <br />
| + | |
| - | See Labeling Protocol: <br />
| + | |
| - | <strong>Freezer Stocks (Glycerol Stocks)</strong>
| + | |
| - | <blockquote>
| + | |
| - | <br />
| + | |
| - | Plasmid Name <br />
| + | |
| - | Backbone <br />
| + | |
| - | Antibiotic Resistance, Cell Type <br />
| + | |
| - | Initials of who prepared the stock, Date</blockquote>
| + | |
| - | If you need to take from the stocks, do not thaw - scrape ice off of the top with a sterile loop and streak across plate.
| + | |
| | <p><strong>Making <em>E-coli</em> glycerol stock:</strong></p> | | <p><strong>Making <em>E-coli</em> glycerol stock:</strong></p> |
| | <p><strong>Material needed:</strong></p> | | <p><strong>Material needed:</strong></p> |
| Line 682: |
Line 27: |
| | <li>Mix briefly by vortexing and store at -80 C </li> | | <li>Mix briefly by vortexing and store at -80 C </li> |
| | </ol> | | </ol> |
| - | <p><br /> | + | <p> |
| - | <br />
| + | |
| | <strong>Growing cells from glycerol stock:</strong> <br /> | | <strong>Growing cells from glycerol stock:</strong> <br /> |
| | With sterile pipet tip, touch the surface of the glycerol stock (DO NOT THAW), and throw the tip in 5 ml LB media with appropriate antibiotics. </p> | | With sterile pipet tip, touch the surface of the glycerol stock (DO NOT THAW), and throw the tip in 5 ml LB media with appropriate antibiotics. </p> |
| | </body> | | </body> |
| | </html> | | </html> |
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