Team:UNITN-Trento/Notebook/Labposts/07/27

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:38, 3 October 2013

{"date" : "2013-07-11","author" : "emil","title" : "The incompetent Bacillus subtilis saga:bacillus medium begins","content" : "Firstable I did an inoculum of Neb10β in order to make them competent the next day.I started to prepare the media needed for the transformation of Bacillus according to LMU munich protocol but due to the failure of this protocol I won't report the protocol.","tags" : "Bacillus subtilis"}

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+{"date" : "2013-07-11","author" : "emil","title" : "The incompetent Bacillus subtilis saga:bacillus medium begins","content" : "<html>Firstable I did an inoculum of Neb10&beta; in order to make them competent the next day.I started to prepare the media needed for the transformation of Bacillus according to LMU munich protocol but due to the failure of this protocol I won't report the protocol.</html>","tags" : "Bacillus subtilis"}
-	"date" : "2013-07-28",
-	"author" : "thomas",
-	"title" : "EFE + Venus Expression test: FAILED!",
-	"content" : "<html>Starting from an inoculum, I diluted the cells 1:100 in fresh LB and I waited until O.D.600 of the sample reached 0.5. I induced then the cells (V=5ml) with 25 ul of Arabinose (5mM) and I incubated them into thermoshaker at 37&deg;C for 4 hours. One sample were not induced and used as negative control.After 4 hours I tried to use the trans-UV too see any differences between induced sample and control. Both culture had the same color (not fluorescence) so the experiment failed. However the sample was sent for sequencing so now I'll wait the result before restart all the cloning steps..</html>",
-	"tags" : "EFE-Venus"
-}