Team:UNITN-Trento/Notebook/Labposts/07/40

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{"date" : "2013-07-18","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : "<html>  today we performed the miniprep protocol on our inocula. yealds are: <br>006 a: 169,3 ng/ul; <br>006 b: 204,4ng/ul; <br>016 a: 204,9 ng/ul; <br>016 b: 173,7 ng/ul; <br>030 a: 276ng/ul; <br>030 b: 375,8ng/ul; <br>082 a: 173,1ng/ul; <br>082 b: 225,9 ng/ul; <br>After that we screened 006b, 016a,082 and 030.<br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 1|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/3f/Tn-2013_a.jpg\" width=\"450px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 2|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/18/Tn.2013_B.jpg\" width=\"450px\" /></center></html>}}<html>As we can see from the gels, we didn’t confirm the presence of any of them!! Now we are going to do a second set of inocula!</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}
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"date" : "2013-07-02",
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"author" : "caterina-michele",
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"title" : "Incredible: E.coli could growth!",
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"content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ",
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"tags" : "PchBA-BMST1-araC-pBAD"
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}
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Latest revision as of 10:41, 3 October 2013

{"date" : "2013-07-18","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : " today we performed the miniprep protocol on our inocula. yealds are:
006 a: 169,3 ng/ul;
006 b: 204,4ng/ul;
016 a: 204,9 ng/ul;
016 b: 173,7 ng/ul;
030 a: 276ng/ul;
030 b: 375,8ng/ul;
082 a: 173,1ng/ul;
082 b: 225,9 ng/ul;
After that we screened 006b, 016a,082 and 030.

gel 1
gel 2
As we can see from the gels, we didn’t confirm the presence of any of them!! Now we are going to do a second set of inocula!","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}

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