Team:UNITN-Trento/Notebook/Labposts/07/45

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Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-20","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : " this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula.
Second set yields are:
006a : 289
006b : 409.3
016° : 188.8
016b :266.6
030a : 388.6
030b : 270
082a : 433.0
082b : 568
Third set yiealds are:
006: 321 ng/ul
016: 440.9 ng/ul
R0082: 441,5 ng/ul
030: 199,9 ng/ul
These ones haven’t been screened so far.
Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}

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+{"date" : "2013-07-20","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : "<html> this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula. <br>Second set yields are: <br>006a : 289 <br>006b : 409.3<br>016° : 188.8<br>016b :266.6<br>030a : 388.6<br>030b : 270<br>082a : 433.0<br>082b : 568<br>Third set yiealds are: <br>006: 321 ng/ul<br>016: 440.9 ng/ul<br>R0082: 441,5 ng/ul<br>030: 199,9 ng/ul<br>These ones haven’t been screened so far. <br>Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}
-	"date" : "2013-07-08",
-	"author" : "emil",
-	"title" : " Purification of Inocula (03/7)",
-	"content" : "<html>I verifyed the status of the last inocula(05/7) that resulted a bit strange.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Inocula|<html><img src=\"\" width=\"450px\" /></html>}}<html>Unfortunately The day before were inoculate uncorrect plates(03/7) so I have purified them following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\"> purification protocol </a> these are the results of the quantification.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1:1) 1</td><td>438.8 ng/&micro;l</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) 2</td><td>400.7 ng/&micro;l</td></tr><tr><td>BBa_K823024+BBa_E0840(1:1) 3</td><td>343.6 ng/&micro;l(the only succesful)</td></tr></table></center></html>}}<html>Afterwards I screened 800 ng of  the samples following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">screening protocol</a> with EcoR1 HF and Pst1, these are the results of the gel:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr><tr><td>Ladder 1kb Fermentas</td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) a</td><td>3</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) b</td><td>4</td></tr><tr><td>BBa_K823024+BBa_E0840(1:1) </td><td>5</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><img src=\"\" width=\"450px\" /></html>}}<html>As we can see only the third(024) sample shows the insert at the right size of 1000 bp(the lower band), then I did the inocula of the plates of 5/07(1:1,1:2,1:3) and of the old plate that gives right results to amplify 024(1:1).</html>",
-	"tags" : "K823024-K823026-E0840"
-}