Team:UNITN-Trento/Notebook/Labposts/07/65

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(Difference between revisions)

Latest revision as of 10:47, 3 October 2013

{"date" : "2013-07-27","author" : "viola-emil","title" : "I can't believe it: BACILLUS IS GROWN!!!!! I think i start to cry now... :) ","content" : "Finally, the problem was pH!!!!! now we can start to transform and transform and transform... {{:Team:UNITN-Trento/Templates/Styles/Spoiler|the faces of happiness:|

}}</html>","tags" : "Bacillus"}

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+{"date" : "2013-07-27","author" : "viola-emil","title" : "<html>I can't believe it: BACILLUS IS GROWN!!!!! I think i start to cry now... :) </html>","content" : "<html>Finally, the problem was pH!!!!! now we can start to transform and transform and transform... {{:Team:UNITN-Trento/Templates/Styles/Spoiler|the faces of happiness:|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/72/Tn-2013_Bacillo.jpg\" /></center></html>}}</html>","tags" : "Bacillus"}
-	"date" : "2013-07-20",
-	"author" : "thomas",
-	"title" : "Digestion of AraC-pBAD + EFE and Venus PCR product",
-	"content" : "<html>This is the part two of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>.Since both Venus (BBa_K537006) and AraC-pBAD + EFE were in pSB1C3 vector, I had to do a PCR on Venus using primers Prefix Fwd and  Suffing Rev following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#pSB1C3-linearization-PCR\">this protocol</a>. The PCR product was then confirmed by an electrophoresis analysis (Venus is 729 bp and KAPA universal Ladder was adopted). <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/f/fe/Tn-2013_VenusPCR_gel.jpg\" style =\"width: 450px\"></center></html>}}<html><br/>The PCR product was then purified and quantified at the NanoDrop.Once I obtained Venus without his backbone, I proceeded digesting all the PRC Venus product with NgoMIV and PstI and 2-3 ug of AraC-pBAD + EFE with AgeI and PstI following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">the PCR product digestion protocol</a>. The digestion products were finally purified, quantified and stored at -20&deg;C.<br/> </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><table class=\"tn-sp-table\"><tr><td>AraC-pBAD + EFE</td><td>33.5 ng/ul</td></tr><tr><td>Venus PCR product</td><td>22.8 ng/ul</td></tr></table></center></html>}}<html></html>",
-	"tags" : "EFE-Venus"
-}