Team:UCSF/lily2
From 2013.igem.org
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<head> | <head> | ||
<!--CSS styles: global--> | <!--CSS styles: global--> | ||
- | < | + | <style type="text/css"> |
- | + | /*** | |
+ | Minimal header: removes the search bar and header image and readjusts font colours in the menus. | ||
+ | |||
+ | Thanks a lot to the 2011 Brown-Stanford and 2012 Lethbridge iGEM teams for snippets of their code! | ||
+ | Check out their wikis at: | ||
+ | https://2011.igem.org/Team:Brown-Stanford | ||
+ | https://2012.igem.org/Team:Lethbridge | ||
+ | ***/ | ||
+ | |||
+ | #content h1.firstHeading { | ||
+ | visibility:hidden; | ||
+ | } | ||
+ | #p-logo { | ||
+ | display: none; | ||
+ | } | ||
+ | #searchform { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .left-menu { | ||
+ | background-color: #555; | ||
+ | |||
+ | } | ||
+ | .left-menu a { | ||
+ | color: #000; | ||
+ | } | ||
+ | |||
+ | div#top-section{ /*the div containing the entire top bar*/ | ||
+ | height: 20px; | ||
+ | margin-bottom: 0px !important; | ||
+ | border: none; | ||
+ | } | ||
+ | |||
+ | |||
+ | #content{ | ||
+ | margin-top: 0px; | ||
+ | } | ||
+ | |||
+ | #search-controls { | ||
+ | overflow:hidden; | ||
+ | display:none; | ||
+ | background: none; | ||
+ | position: absolute; | ||
+ | top: 170px; | ||
+ | right: 40px; | ||
+ | } | ||
+ | |||
+ | |||
+ | div#header { | ||
+ | width: 975px; | ||
+ | text-align: left; | ||
+ | margin-left: auto; | ||
+ | margin-right: auto; | ||
+ | margin-bottom: 0px !important; | ||
+ | } | ||
+ | |||
+ | #menubar { | ||
+ | position: absolute; | ||
+ | background: none; | ||
+ | color: black; | ||
+ | } | ||
+ | |||
+ | .left-menu, .right-menu{ | ||
+ | position: absolute; | ||
+ | background: none; | ||
+ | color: black; | ||
+ | } | ||
+ | |||
+ | .left-menu li a, .right-menu li a { | ||
+ | color: #000 !important; | ||
+ | } | ||
+ | |||
+ | |||
+ | .left-menu ul li, .right-menu ul li a{ | ||
+ | background: none; | ||
+ | color: #000 !important; | ||
+ | } | ||
+ | |||
+ | .left-menu li a:hover, .right-menu li a:hover, .right-menu li a:visited, .right-menu li a:active { | ||
+ | color: #000 !important; | ||
+ | } | ||
+ | |||
+ | #catlinks{ | ||
+ | display:none; | ||
+ | } | ||
+ | |||
+ | /*important for background colours*/ | ||
+ | .mediawiki{ | ||
+ | background: #ffffff; | ||
+ | } | ||
+ | |||
+ | /***End minimal header***/ | ||
+ | |||
+ | /*Base styles*/ | ||
+ | #content{ | ||
+ | border: none; | ||
+ | } | ||
+ | h1, h2,h3, h4, #css-full, #css-mobi{ | ||
+ | font-family: Myriad Pro, Gill Sans MT, Trebuchet MS, Arial, Sans-Serif; | ||
+ | border: 0; | ||
+ | font-weight: 400; | ||
+ | } | ||
+ | |||
+ | p, div.thumb div div.thumbcaption{ | ||
+ | font-family: Calibri, Sans-Serif; | ||
+ | font-weight: normal; | ||
+ | color: black; | ||
+ | margin-bottom: 10px; | ||
+ | padding-left: 5px; | ||
+ | } | ||
+ | |||
+ | #css-full, #css-mobi{ | ||
+ | position: absolute; | ||
+ | float: right; | ||
+ | color: black; | ||
+ | font-size: 1.3em; | ||
+ | top: 0px; | ||
+ | right: 15px; | ||
+ | display: block; | ||
+ | padding: 10px; | ||
+ | } | ||
+ | |||
+ | #jsnotice{ | ||
+ | background-color: #4ED92F; | ||
+ | } | ||
+ | |||
+ | #table{ | ||
+ | margin: 10px; | ||
+ | } | ||
+ | |||
+ | #textlink a:link, | ||
+ | a:visited{ | ||
+ | font-family: Calibri, Sans-Serif; | ||
+ | font-weight: normal; | ||
+ | color: #008000; | ||
+ | text-decoration:none; | ||
+ | } | ||
+ | |||
+ | #textlink a:hover, | ||
+ | a:active{ | ||
+ | font-family: Calibri, Sans-Serif; | ||
+ | font-weight: normal; | ||
+ | color: #008000; | ||
+ | text-decoration:underline; | ||
+ | } | ||
+ | |||
/*====== | /*====== | ||
Desktop Styling | Desktop Styling | ||
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} | } | ||
h2{ | h2{ | ||
- | font-size: 1. | + | font-size: 1.7em; |
+ | } | ||
+ | h3{ | ||
+ | font-size: 1.7em; | ||
} | } | ||
#box1{ | #box1{ | ||
- | width: | + | width: 980px; |
margin-top: -8px; | margin-top: -8px; | ||
background: #fffff; | background: #fffff; | ||
float: left; | float: left; | ||
- | padding-bottom: | + | padding-bottom: 10px; |
- | margin-bottom: | + | margin-bottom: 5px; |
} | } | ||
#box1 h2{ | #box1 h2{ | ||
- | |||
} | } | ||
#box1 p{ | #box1 p{ | ||
- | + | font-size: 1.25em; | |
- | font-size: 1. | + | |
} | } | ||
- | # | + | #UCSFLogo{ |
display: block; | display: block; | ||
float: left; | float: left; | ||
width: 250px; | width: 250px; | ||
- | height: | + | height:200px; |
background: url('https://static.igem.org/mediawiki/2013/6/6b/UCSF2013_Logonew.png'); | background: url('https://static.igem.org/mediawiki/2013/6/6b/UCSF2013_Logonew.png'); | ||
background-position: top; | background-position: top; | ||
- | margin: | + | margin: 40px; |
} | } | ||
+ | /*Floatbox styling*/ | ||
+ | .floatbox | ||
+ | { | ||
+ | width:980px; | ||
+ | float:left; | ||
+ | background-color: #fffff; | ||
+ | margin-top: 10px; | ||
+ | padding-bottom: 10px; | ||
+ | margin-bottom: 5px; | ||
+ | text-align:justify; | ||
+ | } | ||
- | + | /*Showbox Styling*/ | |
- | + | ||
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- | + | ||
- | /* | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | .showbox tr:first-child td { | |
- | + | border-top: 0px; | |
- | + | padding-top: 1px | |
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- | + | .showbox tr:last-child td { | |
- | + | border-bottom: 0px; | |
- | + | padding-bottom: 1px | |
- | + | } | |
- | + | .showbox tr td:first-child { | |
- | + | border-left: 0px; | |
- | + | padding-left: 1px; | |
- | + | } | |
- | + | .showbox tr td:last-child { | |
- | + | border-right: 0px; | |
- | + | padding-right: 1px; | |
- | + | } | |
- | + | ||
- | + | .showbox { | |
- | + | margin-top: -18px; | |
- | + | background:none; | |
- | + | color:#ffffff; | |
- | + | vertical-align:baseline; | |
- | + | border-collapse:collapse; | |
- | + | } | |
- | + | .showbox td { | |
- | + | margin-top: -18px; | |
- | + | background:none; | |
- | + | border:none; | |
- | + | padding:10px; | |
- | + | width:50%; | |
- | + | } | |
- | + | .showbox p { | |
- | + | text-align:top; | |
- | + | color:black; | |
- | + | margin:0px; | |
- | + | font-size: 1.25em; | |
- | + | } | |
- | + | .showbox img { | |
- | + | height:150px; | |
- | + | width:180px; | |
- | + | float:left; | |
- | + | border-radius:4px; | |
- | + | margin-right:10px; | |
- | + | } | |
- | + | a:hover { | |
- | + | text-decoration:none; | |
- | + | } | |
- | + | ||
- | + | /*end showboxstyling */ | |
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</style> | </style> | ||
</head> | </head> | ||
- | |||
+ | <body> | ||
+ | <!--Abstract--> | ||
<div id="box1" align="justify"> | <div id="box1" align="justify"> | ||
- | <a id=" | + | <a id="UCSFLogo"> |
</a> | </a> | ||
- | < | + | <h2><center>Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome</h2></center> |
- | + | ||
<p>In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. The goal of our project is to take advantage of a natural horizontal gene transfer mechanism in bacteria to precisely affect gene expression in selected strains. We combine bacterial conjugation with CRISPRi, an RNAi-like repression system developed from bacteria, to regulate gene expression in targeted strains within a complex microbial community. One possible application is to selectively repress pathogenic genes in a microbiome, leaving the community makeup unaffected. In addition, we use CRISPRi to lay the groundwork for transferring large circuits that enable complex functionality and decision-making in cells. </p> | <p>In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. The goal of our project is to take advantage of a natural horizontal gene transfer mechanism in bacteria to precisely affect gene expression in selected strains. We combine bacterial conjugation with CRISPRi, an RNAi-like repression system developed from bacteria, to regulate gene expression in targeted strains within a complex microbial community. One possible application is to selectively repress pathogenic genes in a microbiome, leaving the community makeup unaffected. In addition, we use CRISPRi to lay the groundwork for transferring large circuits that enable complex functionality and decision-making in cells. </p> | ||
- | + | ||
- | + | ||
</div> | </div> | ||
- | < | + | |
- | < | + | <!--Project Box--> |
- | <img align=" | + | <div class="floatbox"> |
- | < | + | <table class="showbox"> |
+ | <tr> | ||
+ | <td> | ||
+ | |||
+ | <div><a href="https://2013.igem.org/Team:UCSF/About_Us"> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/2/29/2013UCSF_MainBox_Team.jpg" class="blur"></a> | ||
+ | </div> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/About_Us"><h3>Team</h3></a> | ||
+ | <p>Meet our dedicated team of under-graduates who conceived of, planned, and carried out the whole project.</p></a> | ||
+ | </td> | ||
+ | |||
+ | <td> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/Project/Background"><img src="https://static.igem.org/mediawiki/2013/c/c2/UCSF2013_MainBox_Project-1.jpg" class="blur"></a> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/Project/Background"><h3>Project</h3></a> | ||
+ | <p>Discover our precision guided tools to target unique species in a complex microbiome and our groundwork for transferring large circuits that enable complex functionality and decision-making in cells.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/Modeling"> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/a/af/UCSF2013_MainBox_Modeling-1.jpg" class="blur" /> | ||
+ | <!--img src="https://static.igem.org/mediawiki/2013/9/97/UCD_2013_HO_Button.jpg" class="blur"--></a> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/Modeling"><h3>Modeling</h3></a> | ||
+ | <p>Take a look at how we use mathematical modeling to facilitate the design and construct of our project. </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/Exploratorium"><img src="https://static.igem.org/mediawiki/2013/7/7c/UCSF2013_MainBox_HP.jpg" class="blur"</a> | ||
+ | <a href="https://2013.igem.org/Team:UCSF/Exploratorium"><h3>Human Practice</h3></a> | ||
+ | <p>Examine how we teach Synthetic Biology to the general public through night event at Exploratorium and to high school students through a collaboration with Lincoln High School.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <!--Sponsors--> | ||
+ | <div id="box1" align="justify"> | ||
+ | <h2><center>Special Thanks to Our 2013 iGEM Team Sponsors!</h2></center> | ||
+ | <center><img style="margin-bottom:0px; width: 500px; padding:2;"src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Logos/2012%20Sponsors.jpg" usemap="#sponsor"> | ||
+ | <map name="sponsor"> | ||
+ | <area shape="rect" coords="0,0,200,100" href="http://www.lifetechnologies.com/us/en/home.html" /> | ||
+ | <area shape="rect" coords="200,0,450,100" href="http://systemsbiology.ucsf.edu/" /> | ||
+ | <area shape="rect" coords="0,95,180,200" href="http://www.qb3.org/" /> | ||
+ | <area shape="rect" coords="180,95,495,200" href="http://www.synberc.org/" /> | ||
+ | </map> | ||
+ | </center> | ||
+ | </p> | ||
+ | <br> | ||
+ | </div> | ||
+ | |||
</html> | </html> |
Latest revision as of 19:15, 27 September 2013
Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome
In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. The goal of our project is to take advantage of a natural horizontal gene transfer mechanism in bacteria to precisely affect gene expression in selected strains. We combine bacterial conjugation with CRISPRi, an RNAi-like repression system developed from bacteria, to regulate gene expression in targeted strains within a complex microbial community. One possible application is to selectively repress pathogenic genes in a microbiome, leaving the community makeup unaffected. In addition, we use CRISPRi to lay the groundwork for transferring large circuits that enable complex functionality and decision-making in cells.
TeamMeet our dedicated team of under-graduates who conceived of, planned, and carried out the whole project. |
ProjectDiscover our precision guided tools to target unique species in a complex microbiome and our groundwork for transferring large circuits that enable complex functionality and decision-making in cells. |
ModelingTake a look at how we use mathematical modeling to facilitate the design and construct of our project. |
Human PracticeExamine how we teach Synthetic Biology to the general public through night event at Exploratorium and to high school students through a collaboration with Lincoln High School. |