Team:UCSF/Protocols

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<h2><u>Cloning Protocols </u>
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<h1><u>Lab Protocols: </u>Basic Protocols for cloning and work with <i>E. coli</i></center></h1>
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<br><p>
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<a href="https://2013.igem.org/File:2013_UCSF_Gibson_Assembly.pdf#filelinks">Gibson Assembly</a><span> </regulartext></h2>
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<h2>
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<a href="https://static.igem.org/mediawiki/2013/6/66/Colony_PCR.pdf" target="_blank">Colony PCR</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/8/82/Electroporation_Competent_Cells.pdf" target="_blank">Competent Cells Using Electroporation</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/3/3d/E._coli_transformation.pdf" target="_blank"><i>E. coli</i> Transformation</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/c/c6/Gel_Extraction.pdf" target="_blank">Gel Extraction</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/f/fd/Gels.pdf" target="_blank">Gels</a><span> 
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<br>
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<a href="https://static.igem.org/mediawiki/2013/e/ea/2013_UCSF_Gibson_Assembly.pdf" target="_blank">Gibson Assembly</a><span>  
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<br>
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<a href="https://static.igem.org/mediawiki/2013/7/76/In-Fusion_Cloning.pdf" target="_blank">In-Fusion Cloning</a><span>
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<br>
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<a href="<h2>https://static.igem.org/mediawiki/2013/7/7e/Glycerol_Stocks.pdf" target="_blank">Making Glycerol Stocks</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/7/7e/Mini_Prep.pdf" target="_blank">Mini Prep</a><span> <br>
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<a href="https://static.igem.org/mediawiki/2013/e/e0/PCR_Phusion.pdf" target="_blank">PCR Phusion</a><span> <br>
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<a href="https://static.igem.org/mediawiki/2013/9/97/PCR_Purification.pdf" target="_blank">PCR Purification</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/2/24/PCR_Sewing.pdf" target="_blank">PCR Sewing</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/archive/f/f7/20130911200626%21Restriction_Digests.pdf" target="_blank">Restriction Digest</a><span>
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<br>
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<a href="https://static.igem.org/mediawiki/2013/1/13/Seamless_Cloning_and_Assembly.pdf" target="_blank">Seamless Cloning & Assembly</a><span>
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</h2>

Latest revision as of 18:38, 12 September 2013