Team:Groningen/Project/secretion

From 2013.igem.org

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We developed 12 different types of spider silk (Link towards biobricks and check the number) (figure 3). We have one spider silk protein with a N and C terminus, a spider silk without a N and C terminus and a spidersilk without one block (explain the difference). A codon optimization script was developed to codon optimize the spider silk for b. subtilis. Codon optimization (state why we did codon optimization and link towards the codon  optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 4).
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We developed 12 different types of <a href="https://2013.igem.org/Team:Groningen/Navigation/Parts">spider silks</a> (figure 1). We have one spider silk protein with a N and C terminus, a spider silk without a N and C terminus and a spidersilk without one block (explain the difference). A codon optimization script was developed to codon optimize the spider silk for <i>b. subtilis</i>.  
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Codon optimization (state why we did codon optimization and link towards the codon  optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 2).
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<img src="https://static.igem.org/mediawiki/2013/e/ea/Our_silk.png" width="50%">
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<font size="1">Figure 1, Major ampullate Spidroin 2 (MaSp2) from <i>Argiope aurantia</i></font>
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Figure 3, our spider silks
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<img src="https://static.igem.org/mediawiki/2013/7/78/Our_Silks.png" width="100%" >
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<font size="1">Figure 2, Our constructed silk. All <a href="https://2013.igem.org/Team:Groningen/Navigation/Parts">parts</a> are made into biobricks and can be combined together. </font>
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<img src="https://static.igem.org/mediawiki/2013/7/78/Our_Silks.png" width="50%" >
 
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Figure 4: Not sure if this image needs to be on our site, if so please explain thoroughly.
 
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The silk genes are provided with an signal peptide and a strep tag. The signal peptide will ensure the secretion of silk by the natural pathways of b. subtilis (figure 5). The strep tag will be used for our coating mechanism.
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The silk genes are provided with an signal peptide and a strep tag. The signal peptide will ensure the secretion of silk by the natural pathways of b. subtilis (figure 3). The strep tag will be used for our coating mechanism.
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<img src="https://static.igem.org/mediawiki/igem.org/8/82/Sec_bsub.gif" width="401" height="596"></img>
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<font size="1">Figure 3: the secretion pathway of silk </font>
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Figure 5: the secretion pathway of silk
 

Latest revision as of 11:05, 18 September 2013

Silk production

We developed 12 different types of spider silks (figure 1). We have one spider silk protein with a N and C terminus, a spider silk without a N and C terminus and a spidersilk without one block (explain the difference). A codon optimization script was developed to codon optimize the spider silk for b. subtilis. Codon optimization (state why we did codon optimization and link towards the codon optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 2).



Figure 1, Major ampullate Spidroin 2 (MaSp2) from Argiope aurantia

Figure 2, Our constructed silk. All parts are made into biobricks and can be combined together.

The silk genes are provided with an signal peptide and a strep tag. The signal peptide will ensure the secretion of silk by the natural pathways of b. subtilis (figure 3). The strep tag will be used for our coating mechanism.
Figure 3: the secretion pathway of silk

Retrieved from "http://2013.igem.org/Team:Groningen/Project/secretion"