Team:Tokyo-NoKoGen/Project

From 2013.igem.org

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               <div id="title_logo">   
               <div id="title_logo">   
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                    <a href="https://2013.igem.org/Team:Tokyo-NoKoGen"><img class="title" src="https://static.igem.org/mediawiki/2013/4/4e/Title2.png"></a> 
+
                   
        
        
-
                     <a href="https://2013.igem.org"><img class="iGEM2013" src="NoKoGen_material/iGEM2013.png"></a>   
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                     <a href="https://2013.igem.org"><img class="iGEM" src="https://static.igem.org/mediawiki/2013/5/58/IGEM.png"></a>   
               </div>
               </div>
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                         <td class="home1"><div class="home1"></div></td>
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                         <td class="home"><a href="https://2013.igem.org/Team:Tokyo-NoKoGen"><span class="home"></span></a></td>
                          
                          
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                         <td class="project"><a href="https://2013.igem.org/Team:Tokyo-NoKoGen/Project"><span class="project"></span></a></td>
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                         <td class="project1"><div class="project1"></div></td>
                          
                          
                         <td class="team"><a href="https://2013.igem.org/Team:Tokyo-NoKoGen/Team"><span class="team"></span></a></td>
                         <td class="team"><a href="https://2013.igem.org/Team:Tokyo-NoKoGen/Team"><span class="team"></span></a></td>
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                         <td class="sponsors"><a href="https://2013.igem.org/Team:Tokyo-NoKoGen/Sponsors"><span class="sponsors"></span></a></td>
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                         <td class="achievement"><a href="https://2013.igem.org/Team:Tokyo-NoKoGen/Achievement"><span class="achievement"></span></a></td>
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                     </tbody>
                     </table>   
                     </table>   
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               <div id="index">
               <div id="index">
                      
                      
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                     <img class="logo" src="NoKoGen_material/Tシャツ5.png">
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                     <h1 id="index_title">INDEX</h1>
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                     <h1 id="index_title"></h1>
            <ul id="contents">
            <ul id="contents">
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                <a href="#abstract"><li><strong>Abstract</strong></li></a>
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                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/oscillator"><li><a href="#diary"><strong>RNA oscillator</a></strong></li></a>
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                <li><strong></strong></li>
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                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/scaffold"><li><strong>RNA scaffold</strong></li></a>
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                <li><strong></strong></li>
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                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/light"><li><strong>Light sensor</strong></li></a>
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                <li><strong></strong></li>
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                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/modeling"><li><strong>Modeling</strong></li></a>
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                <li><strong></strong></li>
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                <li><strong></strong></li>
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            </ul>
            </ul>
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                            <ul id="contents2">
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                                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/oscillator"><li>RNA oscillator</li></a>
 +
                                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/scaffold"><li>RNAScaffold</li></a>
 +
                                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/light"><li>Light sensor</li></a>
 +
                                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/modeling"><li>Modeling</li></a>
 +
                                <a href="https://2013.igem.org/Team:Tokyo-NoKoGen/rhodopsin"><li>Improving a BioBrick part - Rhodopsin</li></a>
 +
                            </ul>
   
   
               </div>
               </div>
               <div id="main">
               <div id="main">
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                    <img id="mainback" src="https://static.igem.org/mediawiki/2013/f/fd/Twinkle_coli.png">
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<BR>
 +
<BR>
 +
<BR>
 +
<font size=5>
 +
<p align=center><a href=https://2013.igem.org/Team:Tokyo-NoKoGen/overview>Overview</a></p> 
 +
<hr>
 +
<p align=center><a href=https://2013.igem.org/Team:Tokyo-NoKoGen/oscillator>RNA oscillator</a></p> 
 +
<hr>
 +
<p align=center><a href=https://2013.igem.org/Team:Tokyo-NoKoGen/scaffold>RNA scaffold</a></p> 
 +
<hr>
 +
<p align=center><a href=https://2013.igem.org/Team:Tokyo-NoKoGen/light>Light sensor</a></p>
 +
<hr>
 +
<p align=center><a href=https://2013.igem.org/Team:Tokyo-NoKoGen/modeling>Modeling</a></p>
 +
<hr>
 +
<p align=center><a href=https://2013.igem.org/Team:Tokyo-NoKoGen/rhodopsin>Improving a BioBrick part - Rhodopsin</a></p>
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                    <h1 id="abstract">Abstract</h1>
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</font>                    
-
                   
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                    <h2>-Fast cycle! Fast response!-</h2>
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<BR>
 +
 
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<BR>
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<BR>
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<BR>
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              <div id="space">
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              </div>
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                    <p>　This year, we will create Twinkle.coli! Twinkle.coli blinks luminescence like a firefly. To construct it, we need a circuit that oscillates at a short cycle and a fast responsive system. To make oscillating circuit of a short cycle, we designed RNA based oscillator. Some genetic oscillator circuits have been already created and reported. In such common circuits, the oscillation is generated by the expression of inducer and repressor proteins, creating positive and negative feedbacks. As The problem with these oscillators is that because they use proteins to move the oscillation, it takes time for proteins to be transcribed to mRNA, which then needs to be translated to amino acid, and folded into protein, we have to shorten the cycle to make Twinkle coli. To accomplish it, avoid such problem, we chose RNA as a repressor.</p>
 
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                     <p>　The key point in our RNA oscillator is the use of RNA responsible self-cleavage ribozyme, which can control and be controlled by other RNA. To make a fast responsive system, we use the concept of RNA scaffold. When RNA is transcribed as an output from the oscillator, the already translated and existing luciferase and GFP in the cytoplasm bind the transcribed RNA, and BRET occurs which is a shift in luminescence. </p>
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              <div id="footerr">
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                     <ul id="footer_sponsors">
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                        <li class="UI"><a target="_blank" href="http://www.ultizyme.jp/"><img class="UI" src="https://static.igem.org/mediawiki/2013/4/47/アルティザイム・インターナショナル.jpg" ></a></li>
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                        <li class="CB"><a target="_blank" href="http://www.cosmobio.co.jp/index_e.asp"><img class="CB" src="https://static.igem.org/mediawiki/2013/f/fa/コスモバイオ.png"></a></li>
 +
                        <li class="LN"><a target="_blank" href="http://lne.st/"><img class="LN" src="https://static.igem.org/mediawiki/2013/2/2a/リバネス.png"></a></li>
 +
                        <li class="IR"><a target="_blank" href="http://www.ikedarika.co.jp/english/"><img class="IR" src="https://static.igem.org/mediawiki/2013/b/b8/池田理化.gif"></a></li>
 +
                        <li class="Promega"><a target="_blank" href="http://www.promega.com/"><img class="Progma" src="https://static.igem.org/mediawiki/2013/c/c4/Promega.jpg"></li>
 +
                        <li class="IDT"><a target="_blank" href="http://www.idtdna.com/site"><img class="IDT" src="https://static.igem.org/mediawiki/2013/7/71/IDT.png"></li>
 +
                        <li class="MBL"><a target="_blank" href="http://www.mbl.co.jp/e/index.html"><img class="MBL" src="https://static.igem.org/mediawiki/2013/8/85/MBL.gif"></li>
 +
                        <li class="NK"><a target="_blank" href="http://www.tuat.ac.jp/en/index.html"><img class ="NK"src="https://static.igem.org/mediawiki/2013/7/76/東京農工大学.png"></a></li>
 +
                        <li class="ST"><a target="_blank" href="http://www.tuat.ac.jp/~tanpaku/"><img src="https://static.igem.org/mediawiki/2013/5/5a/早出・津川研究室.png" ></a></li>
 +
                        <li class="IK"><a target="_blank" href="http://www.tuat.ac.jp/~kakusan/index.html"><img src="https://static.igem.org/mediawiki/2013/8/87/池袋研究室.png"></a></li>
               </div>
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Latest revision as of 03:41, 28 September 2013

Team:Tokyo-NoKoGen - 2013.igem.org

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