Team:Groningen/Labwork/17 September 2013

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<h2>Claudio</h2>
<h2>Claudio</h2>
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Plates containing pSB1C3-S1-S5-S5-S13 and pSB1C3-S2-S5-S5-S14 showed thousands of colonies.
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Plate containing the ligation product BBa_K1085014-S1-S5-S14 was screened again.
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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
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<br>Colony PCR was performed (NEB Master Mix) on 16 colonies using F-spank-NheI and HM14 primers (annealing temperature 65&deg;C).
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<br>The samples were checked on agarose gel 0.8%.
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<br>The PCR samples were checked on agarose gel 0.8%.
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<br><img src="https://static.igem.org/mediawiki/2013/0/01/ColonyPCR_double5_up_2013-09-15_14hr_38min.tif" width="60%">
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<br>One positive colony (14) was inoculated in LB + Amp.
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<br>Colony C from pSB1C3-S1-S5-S5-S13 and colony A from pSB1C3-S2-S5-S5-S14 showed to be positive candidates and were inoculated in LB + Cm.
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<h2>Sander</h2>
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did transformation to e.coli of BBa_K10850:16, 19, 20, 21, 23, 24, 26 and 28.
<br>
<br>
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<br>Plates containing pSB1C3-S16-S3-S5-S13 and pSB1C3-S16-S9-S11-S14 showed thousands of colonies.
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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
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<h2>Sebas</h2>
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<br>The samples were checked on agarose gel 0.8%.
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<br>The gel didn't run well, however, one colony per plate was inoculated in LB + Cm for further screening.
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Checked sequencing results for vector RFP #16. Results show the RFP and promoter in right position.
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<br>This is now BBa_K1085014 and sent to iGEM HQ.
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Latest revision as of 09:22, 23 September 2013

Claudio

Plate containing the ligation product BBa_K1085014-S1-S5-S14 was screened again.
Colony PCR was performed (NEB Master Mix) on 16 colonies using F-spank-NheI and HM14 primers (annealing temperature 65°C).
The PCR samples were checked on agarose gel 0.8%.
One positive colony (14) was inoculated in LB + Amp.

Sander

did transformation to e.coli of BBa_K10850:16, 19, 20, 21, 23, 24, 26 and 28.

Sebas

Checked sequencing results for vector RFP #16. Results show the RFP and promoter in right position.
This is now BBa_K1085014 and sent to iGEM HQ.