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| - | <div class="article" id="w02-13-05">
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| - | <div class="article-title">
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| - | <p><a name="week02-13-05">Week 2, 13.05.2013, Monday</a></p>
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| - | </div>
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| - | <div class="article-details">
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| - | <p>Posted on 10/06/2013</p>
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| - | </div>
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| - | <div class="article-text">
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| - | <p>
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| - | <b>1) Experiment 1</b><br/>
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| - | We did it!<br/><br/>
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| - | <b>2) Experiment 2</b><br/>
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| - | Succeeded!
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| - | </p>
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| - | </div><!-- /article-text -->
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| - | </div><!-- /article -->
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| - | <div class="article" id="w02-14-05">
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| - | <div class="article-title">
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| - | <p><a name="week02-14-05">Week 2, 14.05.2013, Tuesday</a></p>
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| - | </div>
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| - | <div class="article-details">
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| - | <p>Posted on 10/06/2013</p>
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| - | </div>
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| - | <div class="article-text">
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| - | <p>
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| - | <b>1) Starting with experiment Glycerol dehydrogenase </b><br/>
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| - | - Preparing overnight culture ''E. coli'' KRX <br/><br/>
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| - | <b>2) Experiment 2 - Incubation</b><br/>
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| - | Description of experiment 2
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| - | <br/>
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| - | </p>
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| - | </div><!-- /article-text -->
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| - | </div><!-- /article -->
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| - | <div class="article" id="w02-15-05">
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| - | <div class="article-title">
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| - | <p><a name="week01-15-05">Week 2, 15.05.2013, Wednesday</a></p>
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| - | </div>
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| - | <div class="article-details">
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| - | <p>Posted on 10/06/2013</p>
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| - | </div>
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| - | <div class="article-text">
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| - | <p>
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| - | <b>1) Glycerol dehydrogenase </b><br />
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| - | - Using overnight culture ''E. coli'' KRX <br />
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| - | - Genome Isolation ''E. coli'' KRX using innuPrep Plasmid MiniKit ''JenaAnalytik'' <br />
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| - | - Using ''E. coli'' KRX Genome DNA for PCR <br />
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| - | - PCR-program: <br />
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| - | <!--
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| - | {|cellspacing="0" border="1"
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| - | |'''Component'''
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| - | |'''Volume'''
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| - | |-
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| - | |H2O
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| - | |to 50 μL
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| - | |-
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| - | |5 x Phusion CC Buffer
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| - | |10 μL
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| - | |-
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| - | |10 mM dNTPs
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| - | |2 μL
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| - | |-
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| - | |Fwd and Rev Primer Mix 10 μM
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| - | |2,5 μL
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| - | |-
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| - | |Tempalte 100 ng
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| - | |1 μL
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| - | |DMSO
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| - | |1,5 μL
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| - | |-
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| - | |Phusion DNA Polymerase
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| - | |0,5 μL
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| - | |} -->
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| - | Table PCR-program <br />
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| - | - PCR products are used for agarose gel electrophoresis <br />
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| - | Table gel-program <br />
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| - | - Agarose gel electrophoresis failed, no bands found <br />
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| - | <br /><br />
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| - | <b>2) Experiment 2</b><br />
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| - |
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| - | </p>
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| - | </div><!-- /article-text -->
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| - | </div><!-- /article -->
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| - | <div class="article" id="w02-15-05">
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| - | <div class="article-title">
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| - | <p><a name="week01-09-05">Week 1, 09.05.2013, Thursday</a></p>
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| - | </div>
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| - | <div class="article-details">
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| - | <p>Posted on 10/06/2013</p>
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| - | </div>
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| - | <div class="article-text">
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| - | <p>
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| - | <b>1) Glycerol dehydrogenase </b><br />
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| - | - Using'' E. coli'' KRX Genome DNA for PCR <br />
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| - | - PCR-program: <br />
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| - | Table PCR-components <br />
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| - | Table PCR-program <br />
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| - | - PCR products are used for agarose gel electrophoresis <br />
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| - | Table gel-program <br />
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| - | - Agarose gel electrophoresis worked, see image. <br />
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| - | Picture Agarosegel glda_1 <br />
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| - | - Bands are at expected size of 1050 bp <br />
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| - |
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| - | <br /><br />
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| - | <b>2) Experiment 2</b><br />
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| - |
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| - | </p>
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| - | </div><!-- /article-text -->
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| - | </div><!-- /article -->
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| | | | |
| - | <div class="article" id="w01-10-05">
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| - | <div class="article-title">
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| - | <p><a name="week01-10-05">Week 1, 10.05.2013, Friday</a></p>
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| - | </div>
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| - | <div class="article-details">
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| - | <p>Posted on 10/06/2013</p>
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| - | </div>
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| - | <div class="article-text">
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| - | <p>
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| | | | |
| - | <b> 1) Glycerol dehydrogenase </b> <br />
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| | | | |
| - | - Repeating agarose gel electrophoresis form 23.05.2013 with more sample volume <br />
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| - | - Isolation of bands at 1050 bp using innuPrep Gel Extraction Kit ''JenaAnalytik'' <br />
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| - | - Measurement of DNA-concentration using nanodrop <br />
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| - | <br />
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| - | </p>
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| - | </div><!-- /article-text -->
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| - | </div><!-- /article -->
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| | </div><!-- /page-content --> | | </div><!-- /page-content --> |
| - | </div><!-- /page-middle -->
| + | <!-- </div> /page-middle --> |
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| | </html> | | </html> |
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