Team:Greensboro-Austin/Plasmids and Strains

From 2013.igem.org

(Difference between revisions)
(→Plasmid Database)
(→Primer Database)
 
(28 intermediate revisions not shown)
Line 22: Line 22:
=Primer Database=
=Primer Database=
-
All forward primers are odd numbers and all reverse primers are even numbers. I've paired them up so matching forward and reverse primers are next to each other.
+
I tried putting the forward and reverse primers next to each other to make life easier. Some of the numbering is a little out out of order just because of the timing that they came in... sorry about that
{| cellpadding="5" border="1" cellspacing="0"
{| cellpadding="5" border="1" cellspacing="0"
! Box
! Box
 +
! Slot
! Label
! Label
! Name
! Name
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! Primer
! Primer
! Source
! Source
-
! Sequence
+
! Template
 +
! Homology
 +
! For Construction Of
! Added by
! Added by
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM001
+
|A1
 +
|iGEM001
|fp-151 sfGFP fusion protein forward primer
|fp-151 sfGFP fusion protein forward primer
|
|
|fp_151_sfGFPP_fus_for
|fp_151_sfGFPP_fus_for
|
|
-
|Verified
+
|GTT TAA CTT TAA GAA GGA GAT ATA CAT
 +
|
 +
|
|Kate
|Kate
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM002
+
|A2
 +
|iGEM002
|fp-151 sfGFP fusion protein reverse primer
|fp-151 sfGFP fusion protein reverse primer
|
|
|fp151_sfGFP_homo_fus_rev
|fp151_sfGFP_homo_fus_rev
|
|
-
|Verified
+
|TTG GTA CTA CGC CAG TAA AGA G
 +
|
 +
|
|Kate
|Kate
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM003
+
|B1
 +
|iGEM003
|
|
|
|
|pET21_LacI_for_pCCT_homo
|pET21_LacI_for_pCCT_homo
|
|
-
|Verified
+
|AAA ATA AAC AAA TAG GGG TTC CTT TAG AAA AAT AAA CAA ATC GCC CAC CGG AAG GAG CTG
 +
|
 +
|
|Kate
|Kate
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM004
+
|B2
 +
|iGEM004
|
|
|
|
|pCCT2_rev_pET21_LacI_homo
|pCCT2_rev_pET21_LacI_homo
|
|
-
|Verified
+
|CAG CTC CTT CCG GTG GGC GAT TTG TTT ATT TTT CTA AAG GAA CCC CTA TTT GTT TAT TTT
 +
|
 +
|
|Kate
|Kate
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM005
+
|D3
 +
|iGEM005
|sfGFP ORF forward primer
|sfGFP ORF forward primer
|
|
|sfGFP_ORF_for
|sfGFP_ORF_for
|
|
-
|Verified
+
|GCA TCC AAG GGC GAG GAG C
 +
|
 +
|
|Kate
|Kate
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM006
+
|C2
-
|sfGFP ORF reverse primer
+
|iGEM006
|
|
-
|sfGFP_ORF_rev
+
|
 +
|PET21_T7prom_LacI_Rev
 +
|
 +
|CTT CGG TTT GTA CAT ATG TAT ATC TCC TTC TTA AAG TTA A
|
|
|
|
Line 89: Line 110:
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM007
+
|D1
 +
|iGEM007
|pCCT2 sfGFP ORF forward primer  
|pCCT2 sfGFP ORF forward primer  
|
|
|pCCT2_for_sfGFP_ORF_for
|pCCT2_for_sfGFP_ORF_for
|
|
-
|Verified
+
|CAC CAC CAC CAC TAA CTG GAA TTC GCG GCC GCT TCT AGA G
 +
|
 +
|
|Kate  
|Kate  
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM008
+
|D2
 +
|iGEM008
|pCCT2 sfGFP ORF reverse primer  
|pCCT2 sfGFP ORF reverse primer  
|
|
-
|pCCT2_for_sfGFP_ORF_rev
+
|sfGFP_ORF_rev_PCCT2_
 +
|
 +
|CTC TAG AAG CGG CCG CGA ATT CCA GTT AGT GGT GGT GGT GGT GGT GG
|
|
|
|
Line 107: Line 134:
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM009
+
|E1
 +
|iGEM009
|pSB1C3 Bb forward primer  
|pSB1C3 Bb forward primer  
|
|
|pSB1C3_Bb_Foward
|pSB1C3_Bb_Foward
 +
|
 +
|TACTAGTAGCGGCCGCTGCAGTC
|
|
|
|
Line 116: Line 146:
|-
|-
|iGEM 2013 Primers
|iGEM 2013 Primers
-
|IGM010
+
|E2
 +
|iGEM010
|pSB1C3 Bb reverse primer  
|pSB1C3 Bb reverse primer  
|
|
|pSB1cs_Bbprefix_Reverse
|pSB1cs_Bbprefix_Reverse
 +
|
 +
|CTCTAGAAGCGGCCGCGAATTCCAGAAATC
|
|
|
|
|Kate  
|Kate  
 +
|-
 +
|iGEM 2013 Primers
 +
|F1
 +
|iGEM011
 +
|
 +
|
 +
|BB_prefix_for_tyrosinase
 +
|
 +
|ACT ACA CCT TCG ACG TCT GAC TGG AAT TCG CGG CCG CTT C
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|F2
 +
|iGEM012
 +
|
 +
|
 +
|tyrosinase_rev_BB_prefix
 +
|
 +
|GAA GCG GCC GCG AAT TCC AGT CAG ACG TCG AAG GTG TAG T
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|G1
 +
|MJH 197
 +
|
 +
|
 +
|pSB1C3_bb_for_MjYRS ca
 +
|
 +
|
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|G2
 +
|MJH 198
 +
|
 +
|
 +
|MjYRS_cass_rev_pSB1C3
 +
|
 +
|
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|C1
 +
|iGEM017
 +
|
 +
|
 +
|pET21_T7promRBS_rev
 +
|
 +
|ATG TAT ATC TCC TTC TTA AAG TTA AAC
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|H1
 +
|iGEM018
 +
|
 +
|
 +
|MjYRS_Forward
 +
|
 +
|CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG GCACCTGAAGTCAGCCCCAT
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|H2
 +
|iGEM019
 +
|
 +
|
 +
|MjYRS_Reverse
 +
|
 +
|CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTA TTCGGAATCTTGCACGCCCT
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|I1
 +
|iGEM020
 +
|
 +
|
 +
|ORF438_for_pET_T7pr
 +
|
 +
|
 +
|
 +
|
 +
|Kate
 +
|-
 +
|iGEM 2013 Primers
 +
|I2
 +
|iGEM021
 +
|
 +
|
 +
|RS_internal_seq_for
 +
|
 +
|CCCGGTTTTAACGGGTCTGG
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|J1
 +
|iGEM022
 +
|
 +
|
 +
|RS_5'_seq_for
 +
|
 +
|CTCAAGAAGATCATCTTATTAATCAG
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM023
 +
|
 +
|
 +
|VF2_Biobrick_seqF
 +
|
 +
|CCACCTGACGTCTAAGAAAC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM024
 +
|
 +
|
 +
|tRNA_synth_seq1
 +
|
 +
|GAAGCGATGGGCCTGAAAGCGAAATACGTC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM025
 +
|
 +
|
 +
|tRNA_synth_seq2
 +
|
 +
|CTATATCGCCGACATCACCGATGGG
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM026
 +
|
 +
|
 +
|pSB1C3_seq1
 +
|
 +
|GGTAATACGGTTATCCACAGAATCAGG
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM027
 +
|
 +
|
 +
|pSB1C3_seq2
 +
|
 +
|GATCTTTTCTACGGGGTCTGACGCT
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM028
 +
|
 +
|
 +
|pSB1C3_seq3
 +
|
 +
|GATGAGCATTCATCAGGCGGGCAAG
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM029
 +
|
 +
|
 +
|fp1_5'_NoAmb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM030
 +
|
 +
|
 +
|fp1_5'_2Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM031
 +
|
 +
|
 +
|fp1_5'_5Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM032
 +
|
 +
|
 +
|fp1_5'_10Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM033
 +
|
 +
|
 +
|fp5_NoAmb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM034
 +
|
 +
|
 +
|fp5_4Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM035
 +
|
 +
|
 +
|fp5_7Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM036
 +
|
 +
|
 +
|fp5_10Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM037
 +
|
 +
|
 +
|fp5_20Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM038
 +
|
 +
|
 +
|fp1_3'_NoAmb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM039
 +
|
 +
|
 +
|fp1_3'_2Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM040
 +
|
 +
|
 +
|fp1_3'_5Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM041
 +
|
 +
|
 +
|fp1_3'_9Amb
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM042
 +
|
 +
|
 +
|fp5_for
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM043
 +
|
 +
|
 +
|fp5_rev
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM044
 +
|
 +
|
 +
|5'_fp1_for
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM045
 +
|
 +
|
 +
|5'_fp1_rev
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM046
 +
|
 +
|
 +
|3'_fp1_for
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM047
 +
|
 +
|
 +
|3'_fp1_rev
 +
|
 +
|
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM048
 +
|
 +
|
 +
|MjYRS_Reverse_2
 +
|
 +
|CTT GCC CTT TTT TGC CGG ACT GCA GCG GCC GCT ACT AGT AAG GGC GTG CAA GAT TCC GAA
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM049
 +
|
 +
|
 +
|RemovePstI_F
 +
|
 +
|TACCTGCAAATCAAGAAGATGATCGATCTGCAAAACGCGG
 +
|
 +
|Removes illegal PstI sites from pSB1C3DOPA
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM050
 +
|
 +
|
 +
|RemovePstI_R
 +
|
 +
|CCGCGTTTTGCAGATCGATCATCTTCTTGATTTGCAGGTA
 +
|
 +
|Removes illegal PstI sites from pSB1C3DOPA
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM051
 +
|
 +
|
 +
|RemovePstI_Backbone_F
 +
|
 +
|TGGATGGTGAAGGCAAAATGAGCAG
 +
|
 +
|Use with IGM050 to amplify rest of plasmid without illegal PstI Sites
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM052
 +
|
 +
|
 +
|RemovePstI_Backbone_R
 +
|
 +
|AAGTTGCCTTTGCTGCTGCT
 +
|
 +
|Use with IGM049 to amplify gene without illegal PstI Sites
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM053
 +
|
 +
|
 +
|Deleting_sfGFP_F
 +
|
 +
|CCCC GGATCC CACCACCACCACCACCACTAAC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM054
 +
|
 +
|
 +
|Deleting_sfGFP_R1
 +
|
 +
|CCCC AGATCT TGTTGGTGGATATGTCATCTTCGGC
 +
|
 +
|Use with MAP01-09,12-17
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM055
 +
|
 +
|
 +
|Deleting_sfGFP_R2
 +
|
 +
|CCCC AGATCT TGTTGGTGGCTATGTCATCTTCGGC
 +
|
 +
|Use with MAP10,11,18
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM056
 +
|
 +
|
 +
|MjYRS_synth_seq_1
 +
|
 +
|GAAGCAATGGGGTTAAAGGCAAAATATGTT
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM057
 +
|
 +
|
 +
|
 +
|
 +
| TGG CAC GTA AGA GGT TCC AAC TTT CAC CAT AAT GAA ACA TCT AGA GAA AGA GGA GAA AAA CTC CAT GAC CGT TAC TC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM058
 +
|
 +
|
 +
|
 +
|
 +
| GGG CAT TTC GTA TTT CTC CTC TTT ATT GGT AGG TGG GTT CAT G
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM059
 +
|
 +
|
 +
|
 +
|
 +
| GAA CCC ACC TAC CAA TAA AGA GGA GAA ATA CGA AAT GCC CTC ACT C
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM060
 +
|
 +
|
 +
|
 +
|
 +
| TTC TAC GTT CAT GGC TGA TTT CTC CTC TTT GCG ATC AAG GTT TGG C
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM061
 +
|
 +
|
 +
|
 +
|
 +
| GCC AAA CCT TGA TCG CAA AGA GGA GAA ATC AGC CAT GAA CGT AGA A
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM062
 +
|
 +
|
 +
|
 +
|
 +
| GTC ATT TGG TTT TTC TCC TCT TTG ACA TGA TCA AGC CTC GA
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM063
 +
|
 +
|
 +
|
 +
|
 +
| TCG AGG CTT GAT CAT GTC AAA GAG GAG AAA AAC CAA ATG ACT GAG AAA AAC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM064
 +
|
 +
|
 +
|
 +
|
 +
| GCT CTA GAA GCA TAA TGA TGG TCA GAA TGA ATT GGC GAG G
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM065
 +
|
 +
|
 +
|
 +
|
 +
| TTC ATT CTG ACC ATC ATT ATG CTT CTA GAG CCA GGC A
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM066
 +
|
 +
|
 +
|
 +
|
 +
| ATG TTT CAT TAT GGT GAA AGT TGG AAC CTC TTA CGT GCC AAC CCC TAT TTG TTT ATT TTT CTA AAT
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM067
 +
|
 +
|
 +
|
 +
|
 +
| GAT GAC ATA TCC ACC AAC ACA CCA CCA CCA CCA CCA CTA ACT GGA ATT CGC GGC CG
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM068
 +
|
 +
|
 +
|
 +
|
 +
| TTT GAT GGT TTC CTC GCT TGG ACT CCT GTT GAT AGA TCC AGT AAT GAC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM069
 +
|
 +
|
 +
|
 +
|
 +
| CTA TCA ACA GGA GTC CAA GCG AGG AAA CCA TCA AAA ATT CTG ATT AGA AAA ACT CAT CGA
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM070
 +
|
 +
|
 +
|
 +
|
 +
| CTG GCA TCT TCT AGG ACG TTT CTG CGC TAC CAC TCG TCA TAC GGT TGA TGA GAG CTT TG
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM071
 +
|
 +
|
 +
|
 +
|
 +
| ATG ACG AGT GGT AGC GCA GAA ACG TC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM072
 +
|
 +
|
 +
|
 +
|
 +
| TGG TGG TGG TGT GTT GGT GGA TAT GTC ATC TTC GGC TT
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM073
 +
|
 +
|
 +
|
 +
|
 +
| TGG CAC GTA AGA GGT TCC AAC TTT CAC CAT AAT GAA ACA TCT AGA GAA AGA GGA GAA AAA CTC CAT GAC CGT TAC TC
 +
|
 +
|
 +
|Ben
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM074
 +
|
 +
|
 +
|RemovePstI_F_MjYRS
 +
|
 +
|TACCTGCAAATCAAGAAGATGATCGATCTGCAAAACGCGG
 +
|
 +
|Removes illegal PstI sites from pSB1C3MjYRS
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM075
 +
|
 +
|
 +
|RemovePstI_R_MjYRS
 +
|
 +
|CCGCGTTTTGCAGATCGATCATCTTCTTGATTTGCAGGTA
 +
|
 +
|Removes illegal PstI sites from pSB1C3MjYRS
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM076
 +
|
 +
|
 +
|pSB1C3_F_tobuild_pOS1C1
 +
|
 +
|TCCTAAGGTAGCGAATCCGGCAAAAAAGGG
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM077
 +
|
 +
|
 +
|pSB1C3_R_tobuild_pOS1C1
 +
|
 +
|TATCCCTATCCAGAAATCATCCTTAGCG
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM078
 +
|
 +
|
 +
|Build_pOS1C1_1
 +
|
 +
|ATCCTTAGCTTTCGCTAAGGATGATTTCTGGATAGGGATA
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM079
 +
|
 +
|
 +
|Build_pOS1C1_2
 +
|
 +
|ATGATTTCTGGATAGGGATAACAGGGTAATATTCGCGGCC
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM080
 +
|
 +
|
 +
|Build_pOS1C1_3
 +
|
 +
|ACAGGGTAATATTCGCGGCCGCTTCTAGAGTACTAGTAGC
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM081
 +
|
 +
|
 +
|Build_pOS1C1_4
 +
|
 +
|TTATAGTTACTGCAGCGGCCGCTACTAGTACTCTAGAAGC
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM082
 +
|
 +
|
 +
|Build_pOS1C1_5
 +
|
 +
|GATTCGCTACCTTAGGACCGTTATAGTTACTGCAGCGGCC
 +
|
 +
|
 +
|Razan
 +
|-
 +
|iGEM 2013 Primers
 +
|
 +
|iGEM083
 +
|
 +
|
 +
|Build_pOS1C1_6
 +
|
 +
|CACCTTGCCCTTTTTTGCCGGATTCGCTACCTTAGGACCG
 +
|
 +
|
 +
|Razan
|}
|}
Line 136: Line 1,021:
! Plasmid
! Plasmid
! Source
! Source
-
! Sequence
+
! Template
 +
! Homology
 +
! For Construction Of
! Added by
! Added by
|-
|-
-
|iGEM 2013 Plasmid Swag
+
|iGEM 2013 Plasmid
|A1
|A1
|iGEM 013
|iGEM 013
-
|psB1C3_synthetase_cas_
+
|
 +
|
 +
|pSB1C3_synthetase_cas_
|
|
|
|
Line 149: Line 1,038:
|Kate
|Kate
|-
|-
-
|iGEM 2013 Plasmid Swag
+
|iGEM 2013 Plasmid
|A2
|A2
|iGEM 014
|iGEM 014
 +
|
 +
|
|pSB1C3_Synthetase_Cass
|pSB1C3_Synthetase_Cass
|
|
Line 159: Line 1,050:
|Kate
|Kate
|-
|-
-
|iGEM 2013 Plasmid Swag
+
|iGEM 2013 Plasmid
|A3
|A3
|iGEM 015
|iGEM 015
 +
|
 +
|
|pSB1C3_Synthetase_Cas
|pSB1C3_Synthetase_Cas
|
|
Line 169: Line 1,062:
|Kate
|Kate
|-
|-
-
|iGEM 2013 Plasmid Swag
+
|iGEM 2013 Plasmid
|A4
|A4
|iGEM 016
|iGEM 016
 +
|
 +
|
|pSB1C3_Synthetase_Cass
|pSB1C3_Synthetase_Cass
|
|

Latest revision as of 23:06, 9 September 2013

Primer Database

I tried putting the forward and reverse primers next to each other to make life easier. Some of the numbering is a little out out of order just because of the timing that they came in... sorry about that

Box Slot Label Name Part Primer Source Template Homology For Construction Of Added by
iGEM 2013 Primers A1 iGEM001 fp-151 sfGFP fusion protein forward primer fp_151_sfGFPP_fus_for GTT TAA CTT TAA GAA GGA GAT ATA CAT Kate
iGEM 2013 Primers A2 iGEM002 fp-151 sfGFP fusion protein reverse primer fp151_sfGFP_homo_fus_rev TTG GTA CTA CGC CAG TAA AGA G Kate
iGEM 2013 Primers B1 iGEM003 pET21_LacI_for_pCCT_homo AAA ATA AAC AAA TAG GGG TTC CTT TAG AAA AAT AAA CAA ATC GCC CAC CGG AAG GAG CTG Kate
iGEM 2013 Primers B2 iGEM004 pCCT2_rev_pET21_LacI_homo CAG CTC CTT CCG GTG GGC GAT TTG TTT ATT TTT CTA AAG GAA CCC CTA TTT GTT TAT TTT Kate
iGEM 2013 Primers D3 iGEM005 sfGFP ORF forward primer sfGFP_ORF_for GCA TCC AAG GGC GAG GAG C Kate
iGEM 2013 Primers C2 iGEM006 PET21_T7prom_LacI_Rev CTT CGG TTT GTA CAT ATG TAT ATC TCC TTC TTA AAG TTA A Kate
iGEM 2013 Primers D1 iGEM007 pCCT2 sfGFP ORF forward primer pCCT2_for_sfGFP_ORF_for CAC CAC CAC CAC TAA CTG GAA TTC GCG GCC GCT TCT AGA G Kate
iGEM 2013 Primers D2 iGEM008 pCCT2 sfGFP ORF reverse primer sfGFP_ORF_rev_PCCT2_ CTC TAG AAG CGG CCG CGA ATT CCA GTT AGT GGT GGT GGT GGT GGT GG Kate
iGEM 2013 Primers E1 iGEM009 pSB1C3 Bb forward primer pSB1C3_Bb_Foward TACTAGTAGCGGCCGCTGCAGTC Kate
iGEM 2013 Primers E2 iGEM010 pSB1C3 Bb reverse primer pSB1cs_Bbprefix_Reverse CTCTAGAAGCGGCCGCGAATTCCAGAAATC Kate
iGEM 2013 Primers F1 iGEM011 BB_prefix_for_tyrosinase ACT ACA CCT TCG ACG TCT GAC TGG AAT TCG CGG CCG CTT C Kate
iGEM 2013 Primers F2 iGEM012 tyrosinase_rev_BB_prefix GAA GCG GCC GCG AAT TCC AGT CAG ACG TCG AAG GTG TAG T Kate
iGEM 2013 Primers G1 MJH 197 pSB1C3_bb_for_MjYRS ca Kate
iGEM 2013 Primers G2 MJH 198 MjYRS_cass_rev_pSB1C3 Kate
iGEM 2013 Primers C1 iGEM017 pET21_T7promRBS_rev ATG TAT ATC TCC TTC TTA AAG TTA AAC Kate
iGEM 2013 Primers H1 iGEM018 MjYRS_Forward CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG GCACCTGAAGTCAGCCCCAT Kate
iGEM 2013 Primers H2 iGEM019 MjYRS_Reverse CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTA TTCGGAATCTTGCACGCCCT Kate
iGEM 2013 Primers I1 iGEM020 ORF438_for_pET_T7pr Kate
iGEM 2013 Primers I2 iGEM021 RS_internal_seq_for CCCGGTTTTAACGGGTCTGG Razan
iGEM 2013 Primers J1 iGEM022 RS_5'_seq_for CTCAAGAAGATCATCTTATTAATCAG Razan
iGEM 2013 Primers iGEM023 VF2_Biobrick_seqF CCACCTGACGTCTAAGAAAC Ben
iGEM 2013 Primers iGEM024 tRNA_synth_seq1 GAAGCGATGGGCCTGAAAGCGAAATACGTC Ben
iGEM 2013 Primers iGEM025 tRNA_synth_seq2 CTATATCGCCGACATCACCGATGGG Ben
iGEM 2013 Primers iGEM026 pSB1C3_seq1 GGTAATACGGTTATCCACAGAATCAGG Ben
iGEM 2013 Primers iGEM027 pSB1C3_seq2 GATCTTTTCTACGGGGTCTGACGCT Ben
iGEM 2013 Primers iGEM028 pSB1C3_seq3 GATGAGCATTCATCAGGCGGGCAAG Ben
iGEM 2013 Primers iGEM029 fp1_5'_NoAmb Ben
iGEM 2013 Primers iGEM030 fp1_5'_2Amb Ben
iGEM 2013 Primers iGEM031 fp1_5'_5Amb Ben
iGEM 2013 Primers iGEM032 fp1_5'_10Amb Ben
iGEM 2013 Primers iGEM033 fp5_NoAmb Ben
iGEM 2013 Primers iGEM034 fp5_4Amb Ben
iGEM 2013 Primers iGEM035 fp5_7Amb Ben
iGEM 2013 Primers iGEM036 fp5_10Amb Ben
iGEM 2013 Primers iGEM037 fp5_20Amb Ben
iGEM 2013 Primers iGEM038 fp1_3'_NoAmb Ben
iGEM 2013 Primers iGEM039 fp1_3'_2Amb Ben
iGEM 2013 Primers iGEM040 fp1_3'_5Amb Ben
iGEM 2013 Primers iGEM041 fp1_3'_9Amb Ben
iGEM 2013 Primers iGEM042 fp5_for Ben
iGEM 2013 Primers iGEM043 fp5_rev Ben
iGEM 2013 Primers iGEM044 5'_fp1_for Ben
iGEM 2013 Primers iGEM045 5'_fp1_rev Ben
iGEM 2013 Primers iGEM046 3'_fp1_for Ben
iGEM 2013 Primers iGEM047 3'_fp1_rev Ben
iGEM 2013 Primers iGEM048 MjYRS_Reverse_2 CTT GCC CTT TTT TGC CGG ACT GCA GCG GCC GCT ACT AGT AAG GGC GTG CAA GAT TCC GAA Ben
iGEM 2013 Primers iGEM049 RemovePstI_F TACCTGCAAATCAAGAAGATGATCGATCTGCAAAACGCGG Removes illegal PstI sites from pSB1C3DOPA Razan
iGEM 2013 Primers iGEM050 RemovePstI_R CCGCGTTTTGCAGATCGATCATCTTCTTGATTTGCAGGTA Removes illegal PstI sites from pSB1C3DOPA Razan
iGEM 2013 Primers iGEM051 RemovePstI_Backbone_F TGGATGGTGAAGGCAAAATGAGCAG Use with IGM050 to amplify rest of plasmid without illegal PstI Sites Razan
iGEM 2013 Primers iGEM052 RemovePstI_Backbone_R AAGTTGCCTTTGCTGCTGCT Use with IGM049 to amplify gene without illegal PstI Sites Razan
iGEM 2013 Primers iGEM053 Deleting_sfGFP_F CCCC GGATCC CACCACCACCACCACCACTAAC Ben
iGEM 2013 Primers iGEM054 Deleting_sfGFP_R1 CCCC AGATCT TGTTGGTGGATATGTCATCTTCGGC Use with MAP01-09,12-17 Ben
iGEM 2013 Primers iGEM055 Deleting_sfGFP_R2 CCCC AGATCT TGTTGGTGGCTATGTCATCTTCGGC Use with MAP10,11,18 Ben
iGEM 2013 Primers iGEM056 MjYRS_synth_seq_1 GAAGCAATGGGGTTAAAGGCAAAATATGTT Razan
iGEM 2013 Primers iGEM057 TGG CAC GTA AGA GGT TCC AAC TTT CAC CAT AAT GAA ACA TCT AGA GAA AGA GGA GAA AAA CTC CAT GAC CGT TAC TC Ben
iGEM 2013 Primers iGEM058 GGG CAT TTC GTA TTT CTC CTC TTT ATT GGT AGG TGG GTT CAT G Ben
iGEM 2013 Primers iGEM059 GAA CCC ACC TAC CAA TAA AGA GGA GAA ATA CGA AAT GCC CTC ACT C Ben
iGEM 2013 Primers iGEM060 TTC TAC GTT CAT GGC TGA TTT CTC CTC TTT GCG ATC AAG GTT TGG C Ben
iGEM 2013 Primers iGEM061 GCC AAA CCT TGA TCG CAA AGA GGA GAA ATC AGC CAT GAA CGT AGA A Ben
iGEM 2013 Primers iGEM062 GTC ATT TGG TTT TTC TCC TCT TTG ACA TGA TCA AGC CTC GA Ben
iGEM 2013 Primers iGEM063 TCG AGG CTT GAT CAT GTC AAA GAG GAG AAA AAC CAA ATG ACT GAG AAA AAC Ben
iGEM 2013 Primers iGEM064 GCT CTA GAA GCA TAA TGA TGG TCA GAA TGA ATT GGC GAG G Ben
iGEM 2013 Primers iGEM065 TTC ATT CTG ACC ATC ATT ATG CTT CTA GAG CCA GGC A Ben
iGEM 2013 Primers iGEM066 ATG TTT CAT TAT GGT GAA AGT TGG AAC CTC TTA CGT GCC AAC CCC TAT TTG TTT ATT TTT CTA AAT Ben
iGEM 2013 Primers iGEM067 GAT GAC ATA TCC ACC AAC ACA CCA CCA CCA CCA CCA CTA ACT GGA ATT CGC GGC CG Ben
iGEM 2013 Primers iGEM068 TTT GAT GGT TTC CTC GCT TGG ACT CCT GTT GAT AGA TCC AGT AAT GAC Ben
iGEM 2013 Primers iGEM069 CTA TCA ACA GGA GTC CAA GCG AGG AAA CCA TCA AAA ATT CTG ATT AGA AAA ACT CAT CGA Ben
iGEM 2013 Primers iGEM070 CTG GCA TCT TCT AGG ACG TTT CTG CGC TAC CAC TCG TCA TAC GGT TGA TGA GAG CTT TG Ben
iGEM 2013 Primers iGEM071 ATG ACG AGT GGT AGC GCA GAA ACG TC Ben
iGEM 2013 Primers iGEM072 TGG TGG TGG TGT GTT GGT GGA TAT GTC ATC TTC GGC TT Ben
iGEM 2013 Primers iGEM073 TGG CAC GTA AGA GGT TCC AAC TTT CAC CAT AAT GAA ACA TCT AGA GAA AGA GGA GAA AAA CTC CAT GAC CGT TAC TC Ben
iGEM 2013 Primers iGEM074 RemovePstI_F_MjYRS TACCTGCAAATCAAGAAGATGATCGATCTGCAAAACGCGG Removes illegal PstI sites from pSB1C3MjYRS Razan
iGEM 2013 Primers iGEM075 RemovePstI_R_MjYRS CCGCGTTTTGCAGATCGATCATCTTCTTGATTTGCAGGTA Removes illegal PstI sites from pSB1C3MjYRS Razan
iGEM 2013 Primers iGEM076 pSB1C3_F_tobuild_pOS1C1 TCCTAAGGTAGCGAATCCGGCAAAAAAGGG Razan
iGEM 2013 Primers iGEM077 pSB1C3_R_tobuild_pOS1C1 TATCCCTATCCAGAAATCATCCTTAGCG Razan
iGEM 2013 Primers iGEM078 Build_pOS1C1_1 ATCCTTAGCTTTCGCTAAGGATGATTTCTGGATAGGGATA Razan
iGEM 2013 Primers iGEM079 Build_pOS1C1_2 ATGATTTCTGGATAGGGATAACAGGGTAATATTCGCGGCC Razan
iGEM 2013 Primers iGEM080 Build_pOS1C1_3 ACAGGGTAATATTCGCGGCCGCTTCTAGAGTACTAGTAGC Razan
iGEM 2013 Primers iGEM081 Build_pOS1C1_4 TTATAGTTACTGCAGCGGCCGCTACTAGTACTCTAGAAGC Razan
iGEM 2013 Primers iGEM082 Build_pOS1C1_5 GATTCGCTACCTTAGGACCGTTATAGTTACTGCAGCGGCC Razan
iGEM 2013 Primers iGEM083 Build_pOS1C1_6 CACCTTGCCCTTTTTTGCCGGATTCGCTACCTTAGGACCG Razan

Plasmid Database

Box Slot Label Name Part Plasmid Source Template Homology For Construction Of Added by
iGEM 2013 Plasmid A1 iGEM 013 pSB1C3_synthetase_cas_ Kate
iGEM 2013 Plasmid A2 iGEM 014 pSB1C3_Synthetase_Cass Kate
iGEM 2013 Plasmid A3 iGEM 015 pSB1C3_Synthetase_Cas Kate
iGEM 2013 Plasmid A4 iGEM 016 pSB1C3_Synthetase_Cass Kate
Retrieved from "http://2013.igem.org/Team:Greensboro-Austin/Plasmids_and_Strains"