|
|
| (78 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{:Team:UNIK_Copenhagen/template}}
| |
| - | <html>
| |
| | | | |
| - | <link href='http://fonts.googleapis.com/css?family=The+Girl+Next+Door' rel='stylesheet' type='text/css'>
| |
| - |
| |
| - | <style type="text/css">
| |
| - | #indent_content{
| |
| - | margin: 30px;
| |
| - | padding: 0px;
| |
| - | width: 900px;
| |
| - | }
| |
| - | #all_content p, #all_content h1{
| |
| - | font-family: "The Girl Next Door";
| |
| - | }
| |
| - | #all_content{
| |
| - | font-family: "Helvetica";
| |
| - | margin: 30px;
| |
| - | padding: 0px;
| |
| - | border: 1px solid black;
| |
| - | width: 900px;
| |
| - | height: 570px;
| |
| - | background-color: #000033;
| |
| - | -moz-border-radius: 8px;
| |
| - | -webkit-border-radius: 8px;
| |
| - | }
| |
| - | .content{
| |
| - | height: 546px;
| |
| - | width: 790px;
| |
| - | background-image: url(https://static.igem.org/mediawiki/2013/f/fc/UNIK_Copenhagen_Notebook_Paper.jpg);
| |
| - | background-size: 50%;
| |
| - | border: 1px solid gray;
| |
| - | padding: 10px;
| |
| - | margin: 0px;
| |
| - | }
| |
| - | .left_page, .right_page{
| |
| - | float: left;
| |
| - | width: 375px;
| |
| - | height: 526px;
| |
| - | margin: 10px;
| |
| - | }
| |
| - | #items{
| |
| - | float: left;
| |
| - | padding: 0px;
| |
| - | margin: 1px -41px 1px 41px;
| |
| - | }
| |
| - | #tabs{
| |
| - | margin: 0px;
| |
| - | padding: 0px;
| |
| - | }
| |
| - | #tabs li{
| |
| - | display: block;
| |
| - | list-style: none;
| |
| - | padding: 0px;
| |
| - | margin: 0px;
| |
| - | width: 46px;
| |
| - | height: 46px;
| |
| - | }
| |
| - | #tabs li a{
| |
| - | -moz-border-radius-topright: 10px;
| |
| - | -webkit-border-top-right-radius: 10px;
| |
| - | -moz-border-radius-bottomright: 10px;
| |
| - | -webkit-border-bottom-right-radius: 10px;
| |
| - | text-align: center;
| |
| - | display: block;
| |
| - | font-family: "The Girl Next Door";
| |
| - | font-size: 25px;
| |
| - | color: black;
| |
| - | background-color: #6699cc;
| |
| - | text-decoration: none;
| |
| - | border: 1px solid gray;
| |
| - | border-left: none;
| |
| - | padding: 9px 14px 26px 14px;
| |
| - | }
| |
| - | #tabs li a:hover{
| |
| - | background-color: #e3e3e5;
| |
| - | border: 1px solid #666666;
| |
| - | border-left: none;
| |
| - | }
| |
| - | #tabs li a.active{
| |
| - | background-color: #e3e3e5;
| |
| - | color: black;
| |
| - | border: 1px solid gray;
| |
| - | border-left: none;
| |
| - | }
| |
| - | #content2, #content3, #content4, #content5, #content6, #content7, #content8, #content9, #content10, #content11, #content12{
| |
| - | display: none;
| |
| - | padding: 10px;
| |
| - | }
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <script type="text/javascript">
| |
| - | function ThisTab(new_tab, new_content) {
| |
| - | for(var i=1;i<13;i++){document.getElementById('content'+i).style.display = 'none';}
| |
| - | document.getElementById(new_content).style.display = 'block';
| |
| - |
| |
| - | for(var i=1;i<13;i++){document.getElementById('tab'+i).className = '';}
| |
| - | document.getElementById(new_tab).className = 'active';
| |
| - | }
| |
| - | </script>
| |
| - |
| |
| - | <div id="indent_content">
| |
| - | <h1> Notebook </h1>
| |
| - | <p>Get our Nootebook in a printable version <a href="#"> here </a>.</p>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <div id="all_content">
| |
| - |
| |
| - |
| |
| - | <div id="tabs">
| |
| - | <div id="items">
| |
| - | <div id="content1" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 1 - July 8<sup>th</sup>-14<sup>th</sup> </h1>
| |
| - | <p>
| |
| - | <u>Culturing of magnetotactic bacteria (MTBs)</u> <br>
| |
| - | Making base media for growing magnetotactic bacteria. This media was also used to make agar plates.
| |
| - | MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees.
| |
| - | Plates were kept in a closed container with an Oxoid AnaeroGen pad.<br><br>
| |
| - |
| |
| - | <u>Assembly of constructs</u> <br>
| |
| - | Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates.
| |
| - | Primers for MamC (MS-1 strain) was ordered.
| |
| - | </p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p>Team photo in the park at Frederiksberg Campus </p>
| |
| - | <a href="https://static.igem.org/mediawiki/2013/2/25/UNIK_Copenhagen_slide1.jpeg" target="_blank">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/2/25/UNIK_Copenhagen_slide1.jpeg" width="350">
| |
| - | </a>
| |
| - |
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content2" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 2</h1>
| |
| - | <p><u>Assembly of constructs</u>
| |
| - | Overnight liquid cultures of pBBR1MCS-2 and eGFP plasmid (Adam Takos) <br>
| |
| - | <br>
| |
| - | Touchdown PCR amplification of MamC from MS-1 gDNA (both Phusion and Hotmaster) and of eGFP (only Hotmaster).
| |
| - | <br>
| |
| - | <a href="https://static.igem.org/mediawiki/2013/3/35/UNIK_Copenhagen_Notebook_ref_12.04.jpg" target="_blank">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/3/35/UNIK_Copenhagen_Notebook_ref_12.04.jpg" height="200">
| |
| - | </a>
| |
| - | <br>
| |
| - |
| |
| - | </p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p>Gel purification of bands from PCR amplification and subsequent nanodrop. Due to poor yield from this extraction gradient PCR was performed (56-68 degrees). <br><br>
| |
| - |
| |
| - | <a href="https://static.igem.org/mediawiki/2013/3/30/UNIK_Copenhagen_Notebook_ref_12.20.jpg" target="_blank">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/3/30/UNIK_Copenhagen_Notebook_ref_12.20.jpg" height="200">
| |
| - | </a>
| |
| - | <br><br>
| |
| - |
| |
| - | Traditional cloning of eGFP fragments into pDRIVE.
| |
| - | Transformation of eFbFP-pEX-A (synthesized gene, Eurofins) and eGFP-pDRIVE. </p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content3" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 3</h1>
| |
| - | <p>
| |
| - | <u>Culturing of MTBs</u> <br>
| |
| - | making charcoal media and plates. MS-1 and MSR-1 was inoculated. Liquid cultures were setup in Hungate tubes 200ul, 400ul and 600ul of each strain (MS-1 and MSR-1). Charcoal plates were inoculated as well with both strains.<br>
| |
| - | <u>Assembly of constructs</u><br>
| |
| - | Colony PCR of eFbFP-pEX-A and eGFP-pDRIVE. Only eFbFP was succesful.
| |
| - | <br>
| |
| - | <a href="https://static.igem.org/mediawiki/2013/5/5b/UNIK_Copenhagen_Notebook_ref_12.34.jpg" target="_blank">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/5/5b/UNIK_Copenhagen_Notebook_ref_12.34.jpg" height="150">
| |
| - | </a>
| |
| - | <br>
| |
| - | Overnight cultures were made of colony 1-4 (eFbFP-pEX-A). Miniprep of eFbFP-pEX-A
| |
| - | </p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p>
| |
| - |
| |
| - |
| |
| - | cultures. Samples sent for sequencing. <br><br>
| |
| - | Overnight cultures were made of colony 1-4 (eFbFP-pEX-A).
| |
| - | Miniprep of eFbFP-pEX-A cultures and nanodrop. Samples sent for sequencing.
| |
| - |
| |
| - |
| |
| - | Colony PCR of eGFP-pDRIVE was repeated. Still negative.
| |
| - | Cloning and transformation was also repeated. CloneJET PCR cloning kit was used to create eGFP-pJET (pJET was chosen instead of pDRIVE). Not successful.
| |
| - | <br><br>
| |
| - | Gradient PCR of eGFP premade vector (56-65 degrees). Gel electrophoresis was carried out and the bands were purified from the gel.
| |
| - | <br><br>
| |
| - | Cloning of eGFP into pDRIVE. Not successful.
| |
| - | Cloning of eFbFP into expression vector pBBR1MCS-2.
| |
| - | <br><br>
| |
| - | Colony PCR of eGFP-pDRIVE and eFbFP-pBBR1MCS-2. Gel electrophoresis only reveal one colony to be successful (eFbFP-pBBR1MCS-2 no. 17).
| |
| - | <br><br>
| |
| - | </p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content4" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 4</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content5" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 5</h1>
| |
| - | <p>This week the team . . .</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content6" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 6</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content7" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 7</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content8" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 8</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content9" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 9</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content10" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 10</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content11" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 11</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="content12" class="content">
| |
| - | <div class="left_page">
| |
| - | <h1>Week 12</h1>
| |
| - | <p>This week the team...</p>
| |
| - | </div>
| |
| - | <div class="right_page">
| |
| - | <p> more text on this page!</p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | </div>
| |
| - | <div id="items">
| |
| - | <ul id="tabs">
| |
| - | <li><a href="javascript:ThisTab('tab1', 'content1');" id="tab1" class="active">1</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab2', 'content2');" id="tab2">2</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab3', 'content3');" id="tab3">3</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab4', 'content4');" id="tab4">4</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab5', 'content5');" id="tab5">5</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab6', 'content6');" id="tab6">6</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab7', 'content7');" id="tab7">7</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab8', 'content8');" id="tab8">8</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab9', 'content9');" id="tab9">9</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab10', 'content10');" id="tab10">10</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab11', 'content11');" id="tab11">11</a></li>
| |
| - | <li><a href="javascript:ThisTab('tab12', 'content12');" id="tab12">12</a></li>
| |
| - | </ul>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | </html>
| |
"
