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| - | <p>Nanjing-China 2012 is working on small interfering genetically engineered machine.</p>
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| - | <p>Our team</p>
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| - | <p>Engineered lettuce growing</p>
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| - | <p>Igem LOGO</p>
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| - | <h2>siGEM at a glance</h2>
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| - | <p class="MsoNormal"><span lang="EN-US">The essential and expanding roles of
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| - | small non coding RNAs are becoming clearer to us. More interestingly, plant
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| - | endogenous small RNAs through food intake directly influence gene expression in
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| - | animals (especially in liver) , as a recent study showed. </span></p>
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| - | <p class="MsoNormal"><span lang="EN-US">We took advantage of this
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| - | <span class="word">phenomenon</span>, and made engineered lettuce producing
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| - | artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha,
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| - | whose high expression in liver can cause insulin resistance, as target. (More...
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| - | in project section)</span></p> </div>
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| - | <h2>Parts at a glance</h2>
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| - | <p class="MsoNormal"><span lang="EN-US">Our team designed three main modules for
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| - | our project, si/mi RNA and green plant synthetic biology. They may serve as a
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| - | bit of ground work for future teams working on plant.</span></p>
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| - | <p class="MsoNormal"><span lang="EN-US">They are RNA generating module, T-DNA
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| - | module, and Barcode 2 module. </span></p>
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| - | <p class="MsoNormal"><span lang="EN-US"> RNA generating module make vegetable a
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| - | small RNA producing factory.</span></p>
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| - | <p class="MsoNormal"><span lang="EN-US">Parts in T-DNA module are used for
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| - | constructing customized binary vector for plant.</span></p>
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| - | <p class="MsoNormal"><span lang="EN-US">Barcode 2 module is a signature system
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| - | for engineered plant.</span></p>
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| - | <p class="MsoNormal"><span lang="EN-US"> </span></p>
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| - | <p class="MsoNormal"><span lang="EN-US">(Read more in Parts and project)</span></p>
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| - | <h2>Sharing</h2>
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| - | <p class="MsoNormal"><span lang="EN-US">You can simply use overlapping PCR to
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| - | switch the sequence of small RNAs in part BBa_K788002 to make your own parts and
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| - | plant. (Detail is available in the part registry
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| - | <a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788002">
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| - | BBa_K788002</a>). Also, you can use part<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788010">
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| - | BBa_K788010 </a>and<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788005">
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| - | BBa_K7880005</a> to make new binary vectors. And you may put the signature on
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| - | the plant you work with by adding a barcode 2 part at the end of your system
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| - | according to rules
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| - | <a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788007">
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| - | BBa_K880007</a> gave.</span></p> </div>
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