23/09/13

(Difference between revisions)

Latest revision as of 09:26, 24 September 2013

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Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P);

The gel shows that digestions were successful as every sample dropped the inserted gene.

Gel Order:

F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).

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+==Gel Results from the digestion performed on the [[20/09/13]]==
+[[File:doubledigestion_biobrick_pSB1C3_230913.jpg]]
+Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P);
+*The gel shows that digestions were successful as every sample dropped the inserted gene.
+[[File:igem_BB_230913.jpg]]
+Gel Order:
+F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).
+*Same result as the gel above
+==Evaporation of isolated plasmids and re-suspended in 1xTE==
+*The samples were evaporated with a vacuum evaporator.
+*The samples were re-suspended in 20 ul of 1xTE.
+{|border=1
+|Sample||Volume (ul)||Concentration (ng/ul)||280/260||260/230
+|-
+|B4||20||12.1||1.49||1.44
+|-
+|B9||20||16.3||1.63||1.45
+|-
+|B12||20||26.3||1.87||1.05
+|-
+|X3||20||27.3||1.78||1.11
+|-
+|X5||20||15.6||1.6||1.35
+|-
+|X6||20||25.2||1.63||1.46
+|-
+|F1||20||38.9||1.83||1.57
+|-
+|F10||20||33.6||1.67||1.4
+|-
+|F12||20||6.5||1.48||1.10
+|}
+==Luria Agar plates==
+*Luria agar plates were made for the plating of the CSE kits that arrived.