Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Dailylog

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Latest revision as of 21:02, 21 October 2013

Phage Purification September - October Notebook: October 14 - October 20 Daily Log

Phage Purification

10/16/2013

Took TEMs of mutated T7 phage for the small phage group. Found 3 small mutants and 1 large. We will repeat this experiment to get more measurements on capsid size.

- 10.16 T7 EM

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-: '''Phage Purification September - October Notebook: September 1 - September 14 Daily Log'''</font>
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-: [[Team:BYU_Provo/Phage_Purification|Overview]]
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+: <u> '''Phage Purification''' </u> </font>
 : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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-<html>							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period11/Dailylog"><< Previous</a>
+<html>													<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period11/Dailylog"><< Previous</a>
 							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period3/Dailylog" style="display:block;float:right;">Next >></a>
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-<font size="4"> '''9/4/13''' </font>
+<font size="4"> '''10/16/2013''' </font>
-- Went over the results of our T4 and T7 purification with the Large Phage and small phage group.
+Took TEMs of mutated T7 phage for the small phage group. Found 3 small mutants and 1 large. We will repeat this experiment to get more measurements on capsid size.
-- Updated wiki and discussed plans to have everything completed by the deadlines.
+:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/10.16T7EM| 10.16 T7 EM]]
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-<font size="4"> '''9/6/13''' </font>
-Continued updating wiki.
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-<br>
-<font size="4"> '''9/7/13''' </font>
-We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.
-:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient| 9.7 CsCl Gradient]]
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-<font size="4"> '''9/9/13''' </font>
-We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
-:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
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-<font size="4"> '''9/11/13''' </font>
-- We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
-: -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]
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-<br>
-<font size="4"> '''9/13/13''' </font>
-- We discussed the results from [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]] and determined a new gradient to be run.  Our plan is to run three gradients next class, one wild type and two mutant gradients.
-: Wild type gradient: 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8
-: Mutant gradient 1: 1.2, 1.2960, 1.2966, 1.2975, 1.2981, 1.2989, 1.2997, 1.3, 1.4, 1.6
-: Mutant gradient 2: 1.3, 1.3003, 1.3011, 1.3019, 1.3025, 1.3034, 1.3040, 1.4, 1.5, 1.6
-- We also worked on our team profile and updated the notebook on the wiki.
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