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Team:INSA Toulouse/contenu/lab practice/notebook/calendar/general inducer
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| - | < | + | <h2 class="faq" title="Click to open the FAQ">June 2013</h2> |
| - | + | <div class="infos"> | |
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| - | < | + | |
<ul class="circlearrow"> | <ul class="circlearrow"> | ||
<li><span class="spantitle2">Week 3 (24-30 June)</span></br> | <li><span class="spantitle2">Week 3 (24-30 June)</span></br> | ||
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| + | <h2 class="faq" title="Click to open the FAQ">July 2013</h2> | ||
| + | <div class="infos"> | ||
<ul class="circlearrow"> | <ul class="circlearrow"> | ||
<li><span class="spantitle2">Week 4 (1-7 July)</span><br> | <li><span class="spantitle2">Week 4 (1-7 July)</span><br> | ||
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<img src="https://static.igem.org/mediawiki/2013/e/ef/General_inducer_-_h140px.png" class="imgcontent" /></li> | <img src="https://static.igem.org/mediawiki/2013/e/ef/General_inducer_-_h140px.png" class="imgcontent" /></li> | ||
<li><span class="spantitle2">Week 5 (8-14 July)</span><br> | <li><span class="spantitle2">Week 5 (8-14 July)</span><br> | ||
| - | Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of | + | Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of atc.<br> |
<img src="https://static.igem.org/mediawiki/2013/b/b2/General_inducer2_-_h140px.png" class="imgcontent" /><br> | <img src="https://static.igem.org/mediawiki/2013/b/b2/General_inducer2_-_h140px.png" class="imgcontent" /><br> | ||
The construction is then sent for sequencing.<br><br> | The construction is then sent for sequencing.<br><br> | ||
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We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.<br><br> | We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.<br><br> | ||
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| - | <li><a href="">August 2013</a> | + | <div class="clear"></div> |
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| + | <h2 class="faq" title="Click to open the FAQ">August 2013</h2> | ||
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| + | <li><a href="">August 2013</a> | ||
<ul class="circlearrow"> | <ul class="circlearrow"> | ||
<li><span class="spantitle2">Week 8 (29-4 August)</span><br> | <li><span class="spantitle2">Week 8 (29-4 August)</span><br> | ||
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<img src="https://static.igem.org/mediawiki/2013/5/59/General_inducer5_-_h140px.png" class="imgcontent" /><br> | <img src="https://static.igem.org/mediawiki/2013/5/59/General_inducer5_-_h140px.png" class="imgcontent" /><br> | ||
This one seems to work but we are waiting for the sequencing to confirm the construct.<br><br></li> | This one seems to work but we are waiting for the sequencing to confirm the construct.<br><br></li> | ||
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<img src="https://static.igem.org/mediawiki/2013/3/3a/Back_arrow_-_14px.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/calendar/wet_lab">Back to Wet Lab</a></p> | <img src="https://static.igem.org/mediawiki/2013/3/3a/Back_arrow_-_14px.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/calendar/wet_lab">Back to Wet Lab</a></p> | ||
Latest revision as of 17:35, 4 October 2013
Calendar
General Inducer Characterization
June 2013
- Week 3 (24-30 June)
Amplification of new biobricks for cloning:
BBa_J23119: Strong promoter,
BBa_P0440: rbs tetR term,
BBa_I13521: Ptet rbs RFP term.
July 2013
- Week 4 (1-7 July)
Assembly of a strong promoter with tetR. tetR protein is necessary to inhibit the Ptet promoter.
- Week 5 (8-14 July)
Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of atc.
The construction is then sent for sequencing.
- Week 6 (15-21 July)
The sequencing shows that the construction include a mutated promoter instead of J23119. So we assembled tetR with Ptet-RFP.
- Week 7 (22-28 July)
We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.
August 2013
- August 2013
- Week 8 (29-4 August)
The tries didn’t work, so we did it again one more week the assembly of Strong Promoter with tetR-Ptet-RFP. - Week 9 (5-11 August)
Because none of last clonages worked, we tried with several promoters in order to increase our chances of success.
- Week 10 (12-18 August)
None of the passed assemblies worked, so we tried with a weak promoter.
This one seems to work but we are waiting for the sequencing to confirm the construct.
- Week 8 (29-4 August)
