Team:XMU Software/html

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<div id="lab_s1">
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<div id="lab_s2">
<div id="t1e1">
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18A-AI-2M19
18A-AI-2M19
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<div class="dataOutput">
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<div class="dataOutput1">
<div class="M1_exp_stepInfo" title="AI-2M19" id="sb0s0"> <button class="M1_exp_step_name_button"><input value="AI-2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/7/29 09:15:49--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i0">2013/7/29 09:16:24</td> <td class="M1_exp_item_content"> <table id="i0s0i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="904" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="857" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s0i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s0i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i1">2013/7/30 09:18:19</td> <td class="M1_exp_item_content"> <table id="i0s0i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s0i19"> </textarea></td> </tr> <tr> <td class="C3_td">      </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.80" class="C3_input"></td> <td class="C3_td"><input value="46.4" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.83" class="C3_input"></td> <td class="C3_td"><input value="26.2" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s0i112" name="#i0s0i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i2">2013/7/30 09:19:34</td> <td class="M1_exp_item_content"> <table id="i0s0i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s0i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s0i22M19,18AI.jpg"><br> <form id="i0s0i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s0i22" form="i0s0i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s0i24"> <input name="id" type="hidden" value="i0s0i2" form="i0s0i24"> <input name="oldgraph" type="hidden" id="i0s0i25" form="i0s0i24" value="t1i0s0i22M19,18AI.jpg"> <input id="i0s0i26" type="submit" value="submit" form="i0s0i24" class="C1_button2" disabled=""> <div id="i0s0i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s0i21" class="C1_text">2m19  ai  M500</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i3">2013/7/30 09:21:18</td> <td class="M1_exp_item_content"> <table class="C5" id="i0s0i3"> <tbody><tr> <th colspan="3" id="i0s0i329" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s0i30"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i0s0i330"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i0s0i31" name="#i0s0i3" value="AI (A)"> <input type="button" class="C5_canvas_button2" id="i0s0i32" name="#i0s0i3" value="2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i0s0i33" name="#i0s0i3" value="AI (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i0s0i326" value="0"> <input type="hidden" id="i0s0i327" value="1"> <input type="hidden" id="i0s0i328" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i4">2013/7/30 09:21:31</td> <td class="M1_exp_item_content"> <table id="i0s0i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s0i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s0i4回收2m19,18ai.jpg"><br> <form id="i0s0i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s0i42" form="i0s0i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s0i44"> <input name="id" type="hidden" value="i0s0i4" form="i0s0i44"> <input name="oldgraph" type="hidden" id="i0s0i45" form="i0s0i44" value="t1i0s0i4回收2m19,18ai.jpg"> <input id="i0s0i46" type="submit" value="submit" form="i0s0i44" class="C1_button2" disabled=""> <div id="i0s0i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s0i41" class="C1_text">2M19  M500 AI</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i5">2013/7/30 09:22:23</td> <td class="M1_exp_item_content"> <table id="i0s0i5" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i0s0i56"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct0s0i5"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="0s0i5"> </td> </tr> <tr class="mdoelC5Correct0s0i5"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="1.67" class="C3_input"></td> <td class="C3_td"><input value="18.8" class="C6_input"></td> <td class="C3_td"><input value="2983" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s0i5"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="1.87" class="C3_input"></td> <td class="C3_td"><input value="2.6" class="C6_input"></td> <td class="C3_td"><input value="857" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s0i5" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="0s0i5"> </td> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i0s0i57" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i6">2013/7/30 09:23:12</td> <td class="M1_exp_item_content"> <table id="i0s0i6" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s0i60"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s0i626">A (vector) /μL</th> <td class="C3_td"><input value="1.1061817844701252" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s0i627">B (insert) /μL</th> <td class="C3_td"><input value="6.893818215529875" class="C3_input"></td> <td class="C3_td"><input value="6" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i7">2013/7/31 09:23:55</td> <td class="M1_exp_item_content"><textarea id="i0s0i7" class="C0" rows="1">Transformation</textarea></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="AI-2M19" id="sb0s1"> <button class="M1_exp_step_name_button"><input value="AI-2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/1 09:24:26--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i0">2013/8/1 09:24:42</td> <td class="M1_exp_item_content"> <table id="i0s1i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 1 (6)" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 2 (6)" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s1i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s1i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i1">2013/8/1 09:25:15</td> <td class="M1_exp_item_content"> <table id="i0s1i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s1i19"> </textarea></td> </tr> <tr> <td class="C3_td">      </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.73" class="C3_input"></td> <td class="C3_td"><input value="30.2" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.87" class="C3_input"></td> <td class="C3_td"><input value="41.3" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s1i112" name="#i0s1i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i2">2013/8/1 09:25:39</td> <td class="M1_exp_item_content"> <table id="i0s1i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s1i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s1i213.8.1 18AI-2M19 (2,3).jpg"><br> <form id="i0s1i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s1i22" form="i0s1i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s1i24"> <input name="id" type="hidden" value="i0s1i2" form="i0s1i24"> <input name="oldgraph" type="hidden" id="i0s1i25" form="i0s1i24" value="t1i0s1i213.8.1 18AI-2M19 (2,3).jpg"> <input id="i0s1i26" type="submit" value="submit" form="i0s1i24" class="C1_button2" disabled=""> <div id="i0s1i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s1i21" class="C1_text">AI2M19 1 M500 2
<div class="M1_exp_stepInfo" title="AI-2M19" id="sb0s0"> <button class="M1_exp_step_name_button"><input value="AI-2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/7/29 09:15:49--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i0">2013/7/29 09:16:24</td> <td class="M1_exp_item_content"> <table id="i0s0i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="904" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="857" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s0i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s0i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i1">2013/7/30 09:18:19</td> <td class="M1_exp_item_content"> <table id="i0s0i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s0i19"> </textarea></td> </tr> <tr> <td class="C3_td">      </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.80" class="C3_input"></td> <td class="C3_td"><input value="46.4" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.83" class="C3_input"></td> <td class="C3_td"><input value="26.2" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s0i112" name="#i0s0i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i2">2013/7/30 09:19:34</td> <td class="M1_exp_item_content"> <table id="i0s0i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s0i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s0i22M19,18AI.jpg"><br> <form id="i0s0i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s0i22" form="i0s0i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s0i24"> <input name="id" type="hidden" value="i0s0i2" form="i0s0i24"> <input name="oldgraph" type="hidden" id="i0s0i25" form="i0s0i24" value="t1i0s0i22M19,18AI.jpg"> <input id="i0s0i26" type="submit" value="submit" form="i0s0i24" class="C1_button2" disabled=""> <div id="i0s0i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s0i21" class="C1_text">2m19  ai  M500</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i3">2013/7/30 09:21:18</td> <td class="M1_exp_item_content"> <table class="C5" id="i0s0i3"> <tbody><tr> <th colspan="3" id="i0s0i329" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s0i30"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i0s0i330"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i0s0i31" name="#i0s0i3" value="AI (A)"> <input type="button" class="C5_canvas_button2" id="i0s0i32" name="#i0s0i3" value="2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i0s0i33" name="#i0s0i3" value="AI (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i0s0i326" value="0"> <input type="hidden" id="i0s0i327" value="1"> <input type="hidden" id="i0s0i328" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i4">2013/7/30 09:21:31</td> <td class="M1_exp_item_content"> <table id="i0s0i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s0i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s0i4回收2m19,18ai.jpg"><br> <form id="i0s0i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s0i42" form="i0s0i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s0i44"> <input name="id" type="hidden" value="i0s0i4" form="i0s0i44"> <input name="oldgraph" type="hidden" id="i0s0i45" form="i0s0i44" value="t1i0s0i4回收2m19,18ai.jpg"> <input id="i0s0i46" type="submit" value="submit" form="i0s0i44" class="C1_button2" disabled=""> <div id="i0s0i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s0i41" class="C1_text">2M19  M500 AI</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i5">2013/7/30 09:22:23</td> <td class="M1_exp_item_content"> <table id="i0s0i5" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i0s0i56"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct0s0i5"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="0s0i5"> </td> </tr> <tr class="mdoelC5Correct0s0i5"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="1.67" class="C3_input"></td> <td class="C3_td"><input value="18.8" class="C6_input"></td> <td class="C3_td"><input value="2983" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s0i5"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="1.87" class="C3_input"></td> <td class="C3_td"><input value="2.6" class="C6_input"></td> <td class="C3_td"><input value="857" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s0i5" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="0s0i5"> </td> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s0i5" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i0s0i57" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i6">2013/7/30 09:23:12</td> <td class="M1_exp_item_content"> <table id="i0s0i6" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s0i60"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s0i626">A (vector) /μL</th> <td class="C3_td"><input value="1.1061817844701252" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s0i627">B (insert) /μL</th> <td class="C3_td"><input value="6.893818215529875" class="C3_input"></td> <td class="C3_td"><input value="6" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i7">2013/7/31 09:23:55</td> <td class="M1_exp_item_content"><textarea id="i0s0i7" class="C0" rows="1">Transformation</textarea></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="AI-2M19" id="sb0s1"> <button class="M1_exp_step_name_button"><input value="AI-2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/1 09:24:26--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i0">2013/8/1 09:24:42</td> <td class="M1_exp_item_content"> <table id="i0s1i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 1 (6)" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 2 (6)" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s1i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s1i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i1">2013/8/1 09:25:15</td> <td class="M1_exp_item_content"> <table id="i0s1i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s1i19"> </textarea></td> </tr> <tr> <td class="C3_td">      </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.73" class="C3_input"></td> <td class="C3_td"><input value="30.2" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.87" class="C3_input"></td> <td class="C3_td"><input value="41.3" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s1i112" name="#i0s1i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i2">2013/8/1 09:25:39</td> <td class="M1_exp_item_content"> <table id="i0s1i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s1i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s1i213.8.1 18AI-2M19 (2,3).jpg"><br> <form id="i0s1i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s1i22" form="i0s1i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s1i24"> <input name="id" type="hidden" value="i0s1i2" form="i0s1i24"> <input name="oldgraph" type="hidden" id="i0s1i25" form="i0s1i24" value="t1i0s1i213.8.1 18AI-2M19 (2,3).jpg"> <input id="i0s1i26" type="submit" value="submit" form="i0s1i24" class="C1_button2" disabled=""> <div id="i0s1i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s1i21" class="C1_text">AI2M19 1 M500 2
Line 39: Line 41:
pBAD-2M19
pBAD-2M19
</div>
</div>
-
<div class="dataOutput">
+
<div class="dataOutput2">
<div class="M1_exp_stepInfo" title="pBAD" id="sb1s0"> <button class="M1_exp_step_name_button"><input value="pBAD" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/3 18:01:27--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i0">2013/8/3 18:01:51</td> <td class="M1_exp_item_content"> <table id="i1s0i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s0i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s0i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i1">2013/8/3 18:02:03</td> <td class="M1_exp_item_content"> <table id="i1s0i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s0i19"> </textarea></td> </tr> <tr> <td class="C3_td">      </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.88" class="C3_input"></td> <td class="C3_td"><input value="19" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i1s0i112" name="#i1s0i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i2">2013/8/3 18:03:36</td> <td class="M1_exp_item_content"> <table class="C5" id="i1s0i2"> <tbody><tr> <th colspan="3" id="i1s0i229" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s0i20"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i1s0i230"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i1s0i21" name="#i1s0i2" value="pbad (A)"> <input type="button" class="C5_canvas_button2" id="i1s0i22" name="#i1s0i2" value=" (B)"> <input type="button" class="C5_canvas_button3" id="i1s0i23" name="#i1s0i2" value="pbad (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="0" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="80" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i1s0i226" value="0"> <input type="hidden" id="i1s0i227" value="1"> <input type="hidden" id="i1s0i228" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i3">2013/8/3 18:03:49</td> <td class="M1_exp_item_content"> <table id="i1s0i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s0i30" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s0i313.8.3 牛津样,pBAD.jpg"><br> <form id="i1s0i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s0i32" form="i1s0i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s0i34"> <input name="id" type="hidden" value="i1s0i3" form="i1s0i34"> <input name="oldgraph" type="hidden" id="i1s0i35" form="i1s0i34" value="t1i1s0i313.8.3 牛津样,pBAD.jpg"> <input id="i1s0i36" type="submit" value="submit" form="i1s0i34" class="C1_button2" disabled=""> <div id="i1s0i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s0i31" class="C1_text">OTHER SAMPLE M(500) PBAD
<div class="M1_exp_stepInfo" title="pBAD" id="sb1s0"> <button class="M1_exp_step_name_button"><input value="pBAD" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/3 18:01:27--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i0">2013/8/3 18:01:51</td> <td class="M1_exp_item_content"> <table id="i1s0i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s0i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s0i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i1">2013/8/3 18:02:03</td> <td class="M1_exp_item_content"> <table id="i1s0i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s0i19"> </textarea></td> </tr> <tr> <td class="C3_td">      </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.88" class="C3_input"></td> <td class="C3_td"><input value="19" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i1s0i112" name="#i1s0i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i2">2013/8/3 18:03:36</td> <td class="M1_exp_item_content"> <table class="C5" id="i1s0i2"> <tbody><tr> <th colspan="3" id="i1s0i229" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s0i20"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i1s0i230"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i1s0i21" name="#i1s0i2" value="pbad (A)"> <input type="button" class="C5_canvas_button2" id="i1s0i22" name="#i1s0i2" value=" (B)"> <input type="button" class="C5_canvas_button3" id="i1s0i23" name="#i1s0i2" value="pbad (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="0" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="80" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i1s0i226" value="0"> <input type="hidden" id="i1s0i227" value="1"> <input type="hidden" id="i1s0i228" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i3">2013/8/3 18:03:49</td> <td class="M1_exp_item_content"> <table id="i1s0i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s0i30" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s0i313.8.3 牛津样,pBAD.jpg"><br> <form id="i1s0i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s0i32" form="i1s0i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s0i34"> <input name="id" type="hidden" value="i1s0i3" form="i1s0i34"> <input name="oldgraph" type="hidden" id="i1s0i35" form="i1s0i34" value="t1i1s0i313.8.3 牛津样,pBAD.jpg"> <input id="i1s0i36" type="submit" value="submit" form="i1s0i34" class="C1_button2" disabled=""> <div id="i1s0i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s0i31" class="C1_text">OTHER SAMPLE M(500) PBAD
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</div>
</div>
</div>
</div>
-
 
+
</div>
 +
</div>
<script>
<script>
var dataOutput_tetetetete=-1;
var dataOutput_tetetetete=-1;
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});
});
</script>
</script>
 +
<style>
 +
#menubar{     
 +
display:none;
 +
}
 +
 +
#search-controls{
 +
display:none;
 +
}
 +
input.searchButton{
 +
display:none;
 +
}
 +
#searchGoButton{
 +
display:none;
 +
}
 +
#mw-searchButton{
 +
display:none;
 +
}
 +
</style>
</body>
</body>
</html>
</html>

Latest revision as of 05:18, 27 October 2013

18A-AI-2M19
2013/7/29 09:15:49--Ruosang
2013/7/29 09:16:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/7/30 09:18:19
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/7/30 09:19:34


2013/7/30 09:21:18
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/7/30 09:21:31


2013/7/30 09:22:23
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/7/30 09:23:12
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/7/31 09:23:55
2013/8/1 09:24:26--Ruosang
2013/8/1 09:24:42
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/1 09:25:15
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/1 09:25:39


2013/8/5 21:44:19--Ruosang
2013/8/5 21:45:25
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/5 21:45:55


2013/8/6 21:46:26
2013/8/7 21:50:17
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/7 21:50:56
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/7 21:52:12
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/7 21:52:52
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/8 21:54:37


2013/8/9 22:05:07


2013/8/9 22:13:49
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/9 22:14:05
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:07
2013/8/7 21:46:54--Ruosang
2013/8/7 21:47:35
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/7 21:48:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/7 21:48:47


2013/8/9 22:02:31


2013/8/9 22:15:05
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/9 22:15:18
Cultivation
Plasmid (A )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (B )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (C )
Strain
antibiotics
Medium
Volume
Temperature
Speed
2013/8/10 22:16:21
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:45
2013/8/8 21:56:45--Ruosang
2013/8/8 21:57:12
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/8 22:08:20


2013/8/9 22:09:20--Ruosang
2013/8/9 22:09:38
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:10:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:11:10
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:11:57


2013/8/12 22:22:06--Ruosang
2013/8/12 22:22:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/12 22:22:43
Cultivation
Plasmid (A )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (B )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (C )
Strain
antibiotics
Medium
Volume
Temperature
Speed
2013/8/13 23:21:13
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/13 23:21:47


2013/8/13 23:25:17
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/13 23:36:12


2013/8/15 23:41:26--Ruosang
2013/8/15 23:41:42
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/15 23:44:36
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/15 23:43:57
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/15 23:47:13
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/15 23:52:24
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/15 23:54:43


2013/8/15 23:55:52
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/15 23:56:29
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/16 23:59:03
2013/8/17 23:59:37


2013/8/19 00:07:27


2013/8/19 00:09:02
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/20 00:13:32


2013/8/20 00:14:31--Ruosang
2013/8/20 00:14:48
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/20 00:15:21


2013/8/20 00:16:34


2013/8/20 00:16:50
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/21 00:17:26--Ruosang
2013/8/21 00:17:45
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/21 00:18:04
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/22 00:18:33--Ruosang
2013/8/22 00:18:50
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/22 00:19:07


2013/8/22 00:20:05
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/22 00:20:33
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/23 00:21:17--Ruosang
2013/8/23 00:21:30
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/23 00:21:48


2013/8/23 00:23:10--Ruosang
2013/8/23 00:23:21
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/23 00:24:04
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
(C)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/23 00:24:54


2013/8/23 00:25:28
2013/8/23 00:26:59--Ruosang
2013/8/23 00:27:17
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/23 00:28:07
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/23 00:29:20
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/23 00:29:43
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/24 00:33:05


2013/8/25 00:30:23
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/25 00:30:39
2013/8/26 00:33:48
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/26 00:34:19
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/26 00:31:30


2013/8/28 12:02:00


2013/8/29 11:28:51--Ruosang
2013/8/29 11:29:30
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:30:51
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:32:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:34:47
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:41:40
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:42:42
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:43:26


2013/8/29 11:44:18
2013/8/31 11:44:58


2013/9/2 11:52:55


pBAD-2M19
2013/8/3 18:01:27--Ruosang
2013/8/3 18:01:51
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/3 18:02:03
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/3 18:03:36
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/3 18:03:49


2013/8/3 18:04:55--Ruosang
2013/8/3 18:05:49
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/3 18:08:04
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/3 18:10:12--Ruosang
2013/8/3 18:10:41
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/3 18:11:50
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/3 19:45:37


2013/8/3 18:13:13
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/3 18:13:35
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/3 18:14:18
2013/8/5 11:54:23
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/8 12:43:18


2013/8/9 12:49:26


2013/8/10 22:17:02--Ruosang
2013/8/10 22:17:31
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/10 22:17:54
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/10 22:18:10


2013/8/10 22:18:46
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/10 22:19:07
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/10 22:19:36
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/10 22:20:43
2013/8/13 23:24:12--Ruosang
2013/8/13 23:25:39
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/13 23:28:09
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/13 23:28:51


2013/8/18 00:00:42--Ruosang
2013/8/18 00:01:15
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/18 00:02:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/18 00:02:55
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/18 00:05:05


2013/8/20 00:11:54
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/20 00:10:09


2013/8/31 11:49:18


2013/8/26 00:35:21--Ruosang
2013/8/26 00:35:54
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/26 00:37:04
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/26 00:40:19


2013/8/27 00:42:13
2013/8/27 00:42:54


2013/8/28 11:26:54


2013/9/16 12:56:58
2013/9/16 12:58:29


2013/8/26 00:39:17--Ruosang
2013/8/26 00:39:25
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/26 00:40:04
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/26 00:40:51


2013/8/27 00:42:25
2013/8/28 11:27:57


2013/9/6 12:40:40--Ruosang
2013/9/6 12:41:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/9/6 12:42:40
2013/9/6 12:43:17


2013/9/6 12:44:27
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/9/6 12:44:44
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/9/7 12:48:34


2013/9/7 12:49:01
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/9/7 12:49:19


2013/9/9 12:50:45