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Team:Washington/iGEMVectors
From 2013.igem.org
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<html> | <html> | ||
| - | + | <br> | |
| + | <br> | ||
| + | <blockquote> | ||
<header> | <header> | ||
<b><u>iGEM Vector Information</u></b> | <b><u>iGEM Vector Information</u></b> | ||
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<body> | <body> | ||
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<p> | <p> | ||
| - | + | <table border="1" style="border:1px solid black;border-collapse:collapse;"> | |
| - | <table border="1"> | + | |
<tr> | <tr> | ||
<th>Vector</th> | <th>Vector</th> | ||
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<th>Antibiotic</th> | <th>Antibiotic</th> | ||
<th>Length</th> | <th>Length</th> | ||
| - | <th> | + | <th>Genbank / ApE files</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pSB1C3</td> | + | <td><a href = "http://parts.igem.org/Part:pSB1C3">pSB1C3</td> |
<td>100-300</td> | <td>100-300</td> | ||
| - | <td>ColE1,pMB1(pUC19)</td> | + | <td>ColE1,pMB1 (pUC19)</td> |
<td>Chloramphenicol</td> | <td>Chloramphenicol</td> | ||
<td>2070 bp</td> | <td>2070 bp</td> | ||
| - | <td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/2/20/IGEM_pSB1C3%28std%29.gb">pSB1C3(std).gb</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pSB3K3</td> | + | <td><a href = "http://parts.igem.org/Part:pSB1A3">pSB1A3</td> |
| + | <td>100-300</td> | ||
| + | <td>ColE1,pMB1 (pUC19)</td> | ||
| + | <td>Ampicillin</td> | ||
| + | <td>2155 bp</td> | ||
| + | <td><a href = "https://static.igem.org/mediawiki/2013/4/45/IGEM_pSB1A3%28std%29.gb">pSB1A3(std).gb</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><a href = "http://parts.igem.org/Part:pSB3K3">pSB3K3</td> | ||
<td>20-30</td> | <td>20-30</td> | ||
| - | <td>p15A(pMR101)</td> | + | <td>p15A (pMR101)</td> |
<td>Kanamycin</td> | <td>Kanamycin</td> | ||
<td>2750 bp</td> | <td>2750 bp</td> | ||
| - | <td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/3/3d/IGEM_pSB3K3%28std%29.gb">pSB3K3(std).gb</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pSB4A5</td> | + | <td><a href = "http://parts.igem.org/Part:pSB4A5">pSB4A5</td> |
<td>~5</td> | <td>~5</td> | ||
| - | <td>pSC101</td> | + | <td>RepA (pSC101)</td> |
| - | <td>Ampicillin </td> | + | <td>Ampicillin</td> |
<td>2037 bp</td> | <td>2037 bp</td> | ||
| - | <td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/7/73/IGEM_pSB4A5%28std%29.gb">pSB4A5(std).gb</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pSB3T5</td> | + | <td><a href = "http://parts.igem.org/Part:pSB3T5">pSB3T5</td> |
| - | <td> | + | <td>20-30</td> |
| - | <td> | + | <td>p15A (pMR101)</td> |
| - | <td> | + | <td>Tetracycline</td> |
| - | <td> | + | <td>3252 bp</td> |
| - | <td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB3T5(std).gb</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| - | |||
| - | |||
| - | <br> | + | Vector files are in Genbank compatible ApE format saved as .gb files. <br> |
| + | The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments. | ||
| + | </p> | ||
| + | <header> | ||
| + | <b>Postive Controls (with marker):</b> | ||
| + | </header> | ||
| + | <a href = "https://static.igem.org/mediawiki/2013/2/2e/UW_pSB1C3%28std%29-sfGFP.gb">pSB1C3(std)-sfGFP.gb<br> | ||
| + | <a href = "https://static.igem.org/mediawiki/2013/8/8c/UW_pSB1A3%28std%29-J23118-mRFP.gb">pSB1A3(std)-J23118-mRFP.gb<br/> | ||
| + | <a href = "https://static.igem.org/mediawiki/2013/7/7d/UW_pSB3K3%28std%29-sfGFP.gb">pSB3K3(std)-sfGFP.gb<br> | ||
| + | <a href = "https://static.igem.org/mediawiki/2013/9/95/UW_pSB4A5%28std%29-J23114-Plac-RFP.gb">pSB4A5(std)-J23114-Plac-RFP.gb<br> | ||
| + | <a href = "https://static.igem.org/mediawiki/2013/c/c4/UW_pSB4A5%28std%29-J23114-Plac-RFP-simple.gb">pSB4A5(std)-J23114-Plac-RFP-simple.gb<br> | ||
| + | <a href = "https://static.igem.org/mediawiki/2013/0/0d/UW_pSB3T5%28std%29-Plac-RFP.gb">pSB3T5(std)-Plac-RFP.gb<br> | ||
| + | <br/><br/> | ||
</body> | </body> | ||
| - | |||
</html> | </html> | ||
Latest revision as of 20:33, 27 September 2013
iGEM Vector Information
Vector files are in Genbank compatible ApE format saved as .gb files.
Vector Copy Number Equivalent Antibiotic Length Genbank / ApE files pSB1C3 100-300 ColE1,pMB1 (pUC19) Chloramphenicol 2070 bp pSB1C3(std).gb pSB1A3 100-300 ColE1,pMB1 (pUC19) Ampicillin 2155 bp pSB1A3(std).gb pSB3K3 20-30 p15A (pMR101) Kanamycin 2750 bp pSB3K3(std).gb pSB4A5 ~5 RepA (pSC101) Ampicillin 2037 bp pSB4A5(std).gb pSB3T5 20-30 p15A (pMR101) Tetracycline 3252 bp pSB3T5(std).gb
The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.Postive Controls (with marker): pSB1C3(std)-sfGFP.gb
pSB1A3(std)-J23118-mRFP.gb
pSB3K3(std)-sfGFP.gb
pSB4A5(std)-J23114-Plac-RFP.gb
pSB4A5(std)-J23114-Plac-RFP-simple.gb
pSB3T5(std)-Plac-RFP.gb
