Team:Washington/iGEMVectors

From 2013.igem.org

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<b><u>iGEM Vector Information</u></b>
<b><u>iGEM Vector Information</u></b>
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<table>
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<table border="1" style="border:1px solid black;border-collapse:collapse;">
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<tr>
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   <th>Vector</th>
   <th>Vector</th>
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   <th>Antibiotic</th>
   <th>Antibiotic</th>
   <th>Length</th>
   <th>Length</th>
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   <th>Map</th>
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   <th>Genbank / ApE files</th>
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   <td>pSB1C3</td>
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   <td><a href = "http://parts.igem.org/Part:pSB1C3">pSB1C3</td>
   <td>100-300</td>
   <td>100-300</td>
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   <td>ColE1,pMB1(pUC19)</td>
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   <td>ColE1,pMB1 (pUC19)</td>
   <td>Chloramphenicol</td>
   <td>Chloramphenicol</td>
   <td>2070 bp</td>
   <td>2070 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/2/20/IGEM_pSB1C3%28std%29.gb">pSB1C3(std).gb</td>
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   <td>pSB3K3</td>
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   <td><a href = "http://parts.igem.org/Part:pSB1A3">pSB1A3</td>
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  <td>100-300</td>
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  <td>ColE1,pMB1 (pUC19)</td>
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  <td>Ampicillin</td>
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  <td>2155 bp</td>
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  <td><a href = "https://static.igem.org/mediawiki/2013/4/45/IGEM_pSB1A3%28std%29.gb">pSB1A3(std).gb</td>
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  <td><a href = "http://parts.igem.org/Part:pSB3K3">pSB3K3</td>
   <td>20-30</td>
   <td>20-30</td>
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   <td>p15A(pMR101)</td>
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   <td>p15A (pMR101)</td>
   <td>Kanamycin</td>
   <td>Kanamycin</td>
   <td>2750 bp</td>
   <td>2750 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/3/3d/IGEM_pSB3K3%28std%29.gb">pSB3K3(std).gb</td>
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   <td>pSB4A5</td>
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   <td><a href = "http://parts.igem.org/Part:pSB4A5">pSB4A5</td>
   <td>~5</td>
   <td>~5</td>
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   <td>RepA(pSC101)</td>
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   <td>RepA (pSC101)</td>
   <td>Ampicillin</td>
   <td>Ampicillin</td>
   <td>2037 bp</td>
   <td>2037 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/7/73/IGEM_pSB4A5%28std%29.gb">pSB4A5(std).gb</td>
</tr>
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   <td>pSB3T5</td>
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   <td><a href = "http://parts.igem.org/Part:pSB3T5">pSB3T5</td>
   <td>20-30</td>
   <td>20-30</td>
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   <td>p15A(pMR101)</td>
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   <td>p15A (pMR101)</td>
   <td>Tetracycline</td>
   <td>Tetracycline</td>
   <td>3252 bp</td>
   <td>3252 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB3T5(std).gb</td>
</tr>
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</table>
</table>
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Vector files are in Genbank compatible ApE format saved as .gb files. <br>
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The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.
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<br>
 
<header>
<header>
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<b><u>Vector Equivalency Chart</u></b>
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<b>Postive Controls (with marker):</b>
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</header>
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<a href = "https://static.igem.org/mediawiki/2013/2/2e/UW_pSB1C3%28std%29-sfGFP.gb">pSB1C3(std)-sfGFP.gb<br>
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<table>
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<a href = "https://static.igem.org/mediawiki/2013/8/8c/UW_pSB1A3%28std%29-J23118-mRFP.gb">pSB1A3(std)-J23118-mRFP.gb<br/>
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<table border="1">
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<a href = "https://static.igem.org/mediawiki/2013/7/7d/UW_pSB3K3%28std%29-sfGFP.gb">pSB3K3(std)-sfGFP.gb<br>
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<tr>
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<a href = "https://static.igem.org/mediawiki/2013/9/95/UW_pSB4A5%28std%29-J23114-Plac-RFP.gb">pSB4A5(std)-J23114-Plac-RFP.gb<br>
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  <th>Source Vector</th>
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<a href = "https://static.igem.org/mediawiki/2013/c/c4/UW_pSB4A5%28std%29-J23114-Plac-RFP-simple.gb">pSB4A5(std)-J23114-Plac-RFP-simple.gb<br>
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  <th>Origin</th>
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<a href = "https://static.igem.org/mediawiki/2013/0/0d/UW_pSB3T5%28std%29-Plac-RFP.gb">pSB3T5(std)-Plac-RFP.gb<br>
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  <th>Copy Number</th>
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  <th>Antibiotic</th>
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  <th>Biobrick Equivalent</th>
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  <th>Antibiotic</th>
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<tr>
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  <td>pJT119b</td>
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  <td>ColE1,pMB1</td>
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  <td>100-300</td>
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  <td>Chloramphenicol</td>
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  <td>pSB1C3</td>
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  <td>Chloramphenicol</td>
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</tr>
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<tr>
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  <td>pPLPCB</td>
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  <td>p15A</td>
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  <td>20-30</td>
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  <td>Spectinomycin</td>
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  <td>pSB3T5</td>
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  <td>Tetracycline</td>
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<tr>
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  <td>pEO100c</td>
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  <td>pSC101</td>
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  <td>~5</td>
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  <td>Ampicillin</td>
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  <td>pSB4A5</td>
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  <td>Ampicillin</td>
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  <td>pJT106b</td>
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  <td>pSC101</td>
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  <td>~5</td>
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  <td>Ampicillin</td>
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  <td>pSB4A5</td>
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  <td>Ampicillin</td>
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</tr>
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</table>
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Latest revision as of 20:33, 27 September 2013



iGEM Vector Information

Vector Copy Number Equivalent Antibiotic Length Genbank / ApE files
pSB1C3 100-300 ColE1,pMB1 (pUC19) Chloramphenicol 2070 bp pSB1C3(std).gb
pSB1A3 100-300 ColE1,pMB1 (pUC19) Ampicillin 2155 bp pSB1A3(std).gb
pSB3K3 20-30 p15A (pMR101) Kanamycin 2750 bp pSB3K3(std).gb
pSB4A5 ~5 RepA (pSC101) Ampicillin 2037 bp pSB4A5(std).gb
pSB3T5 20-30 p15A (pMR101) Tetracycline 3252 bp pSB3T5(std).gb
Vector files are in Genbank compatible ApE format saved as .gb files.
The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.

Postive Controls (with marker):
pSB1C3(std)-sfGFP.gb
pSB1A3(std)-J23118-mRFP.gb
pSB3K3(std)-sfGFP.gb
pSB4A5(std)-J23114-Plac-RFP.gb
pSB4A5(std)-J23114-Plac-RFP-simple.gb
pSB3T5(std)-Plac-RFP.gb


Retrieved from "http://2013.igem.org/Team:Washington/iGEMVectors"