Team:Groningen/20 June 2013

From 2013.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
-
Started the restriction digest on biobrick BBa_K844016,
+
'''Mirjam, Inne and Erik'''
 +
<br/>Added 1 ul Serva/100 ml 1.5% agarose gel.
 +
<br/>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water.
 +
<br/>Made a 2x dilution of the loading buffer.
-
<br>EcoRI
+
The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made:
-
<br>XbaI
+
<br/>7 ul MQ water
-
<br>SpeI
+
<br/>1 ul 10x buffer
-
<br>PstI
+
<br/>1 ul restriction enzyme
-
<br>BfaI
+
<br/>2 ul DNA solution
-
10:59 put tubes in in 37 C incubator
+
The incubation is done at 37 degrees Celsius for 2 hours.
 +
 
 +
After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel.
 +
<br/>The gel electrophorese is done for 25 minutes at 90V.
 +
 
 +
The picture is coming soon.

Latest revision as of 11:12, 28 June 2013

Mirjam, Inne and Erik
Added 1 ul Serva/100 ml 1.5% agarose gel.
Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water.
Made a 2x dilution of the loading buffer.

The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made:
7 ul MQ water
1 ul 10x buffer
1 ul restriction enzyme
2 ul DNA solution

The incubation is done at 37 degrees Celsius for 2 hours.

After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel.
The gel electrophorese is done for 25 minutes at 90V.

The picture is coming soon.

Retrieved from "http://2013.igem.org/Team:Groningen/20_June_2013"