Team:Ciencias-UNAM/Project/Conclusions

From 2013.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 248: Line 248:
</div>
</div>
<div id="u61" class="u61"  >
<div id="u61" class="u61"  >
-
<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Conclusions</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Sed facilisis eleifend nunc, quis luctus diam porta non. Nunc elementum nibh erat, placerat tincidunt odio iaculis id. Morbi pharetra dolor tellus, non porttitor nisi congue at. Sed ligula tortor, malesuada nec pulvinar pharetra, tincidunt in massa. Nunc fringilla metus mattis purus pretium dignissim. Fusce sed neque augue. Proin iaculis urna lacus, sed egestas metus ultrices vitae.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Quisque tristique, mi non fermentum sodales, lacus odio dapibus ante, et placerat elit mauris vitae tortor. Curabitur odio erat, lobortis eget pulvinar vitae, venenatis quis tortor. Ut nisl magna, porttitor ullamcorper mollis quis, pulvinar eu est. Aliquam in consectetur enim. Vestibulum eleifend ante nec arcu pharetra feugiat. Cras lobortis ligula sed sapien gravida, tempor condimentum magna dictum. Nulla sit amet ante nibh. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nulla velit urna, aliquet ac tellus eu, interdum semper lorem. In tortor urna, molestie quis sodales sit amet, vehicula et dolor. Donec vel turpis mi. Fusce nec tellus sit amet nibh iaculis sagittis. Mauris ultrices malesuada sem vel tempus. Ut tristique, ante eget condimentum rhoncus, purus metus elementum eros, a tempor ligula nisi quis diam.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Nulla consequat vitae dui id condimentum. Phasellus in placerat mauris. Donec id sapien commodo, feugiat nisi ut, suscipit justo. Curabitur dignissim nulla purus. Phasellus vestibulum ornare sem, ut consectetur elit vestibulum eu. Pellentesque convallis, risus ac vulputate tempus, urna dui bibendum lorem, ut tincidunt justo arcu dapibus ipsum. Integer pellentesque eleifend consequat. Suspendisse hendrerit ante vitae leo sagittis condimentum. Quisque sed convallis ipsum, eu accumsan nisl. Morbi lacus lectus, faucibus eget placerat vel, dignissim ut diam. Ut suscipit a erat non blandit. Cras sit amet tincidunt tellus. Donec pulvinar neque eget iaculis venenatis. Praesent ut tortor dolor. Vestibulum ullamcorper auctor mauris sit amet rhoncus.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Pellentesque adipiscing, arcu vel consectetur vehicula, felis ligula pulvinar tellus, vitae dignissim mi leo ut velit. Cras placerat in risus posuere commodo. Maecenas mattis purus ac est sodales pellentesque. Sed felis magna, euismod eu est in, faucibus bibendum nisl. Praesent rutrum tortor odio, in feugiat lacus facilisis nec. Cras enim ante, mattis sit amet placerat a, dapibus posuere ipsum. Aenean at libero nulla. Vivamus nec mi aliquet, malesuada libero ac, tincidunt elit. In hac habitasse platea dictumst. Aenean aliquet id augue id accumsan. Curabitur tincidunt, nunc id adipiscing gravida, dolor nisl auctor ante, eu rutrum tellus ipsum non turpis. Nam rutrum.</span></p></div>
+
<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Conclusions</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br>
 +
Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.
 +
<br>
 +
<b>MarA</b> Showed to increase the sensibility to Tetracyclin.<br>
 +
Since the plasmid in which the part is transformed uses the Cloramphenicol resistance, it is possible that due to the similar action mechanism they both use, causes an hypersensitivity. Still, MarA showed providing resistance to Kanamycin. This is clearly shown in the graphs in the results section.
 +
</span></p></div>
</div>
</div>
</div>
</div>

Latest revision as of 03:53, 28 September 2013

Conclusions

HOME

PROJECT

HUMAN PRACTICES

TEAM

SAFETY

Home > Project > Conclusions

PROJECT MENU

Overview

General Design

WetLab

BioBricks

Results

Characterization

Protocols

DryLab

Modelling

Conclusions

Future Work

References

Conclusions


Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.
MarA Showed to increase the sensibility to Tetracyclin.
Since the plasmid in which the part is transformed uses the Cloramphenicol resistance, it is possible that due to the similar action mechanism they both use, causes an hypersensitivity. Still, MarA showed providing resistance to Kanamycin. This is clearly shown in the graphs in the results section.

Retrieved from "http://2013.igem.org/Team:Ciencias-UNAM/Project/Conclusions"