Team:Freiburg/main2

From 2013.igem.org

(Difference between revisions)
 
(130 intermediate revisions not shown)
Line 10: Line 10:
width:100%;
width:100%;
margin:auto;
margin:auto;
 +
color:white;
}
}
Line 16: Line 17:
}
}
-
#cas{
+
#table_viereck tr td ul{
-
height:250px;
+
list-style: none;
float:left;
float:left;
-
margin-top:70px;
+
z-index:2
}
}
-
#klammer_gross{
+
#table_viereck tr td ul li img{
-
height:300px;
+
height:60px;
 +
}
 +
 
 +
#klammer_gross_links{
 +
height:350px;
float:left;
float:left;
padding-top:3px;
padding-top:3px;
}
}
-
#activation{
+
 
-
height:50px;
+
#klammer_gross_rechts{
 +
height:350px;
 +
float: left;
 +
padding-top:3px;
 +
}
 +
 
 +
#klammer_klein_links{
 +
height:175px;
 +
width:45px;
float:left;
float:left;
 +
padding-top:3px;
}
}
 +
 +
#klammer_klein_rechts{
 +
height:175px;
 +
width:45px;
 +
float: left;
 +
padding-top:3px;
 +
}
 +
#text_gross{
#text_gross{
Line 36: Line 58:
font-family:'Yanone';
font-family:'Yanone';
font-size:50px;
font-size:50px;
 +
padding-top:2px;
 +
}
 +
 +
#text{
 +
color:white;
 +
width:320px;
 +
height:200px;
padding-top:5px;
padding-top:5px;
 +
font-family:Arial;
 +
font-size:10px;
float:left;
float:left;
}
}
-
#text{
+
#text-box2{
color:white;
color:white;
-
width:300px;
+
width:500px;
-
padding-top:70px;
+
height:300px;
 +
padding-top:5px;
font-family:Arial;
font-family:Arial;
font-size:10px;
font-size:10px;
float:left;
float:left;
 +
background: url(https://static.igem.org/mediawiki/2013/9/94/Freiburg_2013_main2_Cas.png);
 +
background-size: 250px;
 +
background-position:120px 150px;
 +
background-repeat: no-repeat;
}
}
 +
 +
#text-box2 ul li span{
 +
font-size: 20px;
 +
font-family:'Yanone';
 +
}
 +
 +
#text-box3-4{
 +
color:white;
 +
width:170px;
 +
padding-top:5px;
 +
font-family:Arial;
 +
font-size:10px;
 +
float:left;
 +
}
 +
 +
#text-box5-6{
 +
color:white;
 +
width:110px;
 +
padding-top:5px;
 +
font-family:Arial;
 +
font-size:10px;
 +
float:left;
 +
}
 +
@font-face {
@font-face {
Line 59: Line 119:
<div style="min-height:800px; margin-top:230px;" >
<div style="min-height:800px; margin-top:230px;" >
 +
 +
<!-- ########################################################################################
 +
 +
TABLE OBEN
 +
 +
######################################################################################## -->
 +
 +
  <table id="table_viereck">
  <table id="table_viereck">
 +
 +
<!-- ########################################################################################
 +
 +
ABSTRACT
 +
 +
######################################################################################## -->
 +
 +
<tr>
<tr>
-
<td>
+
 
-
   <img id="klammer_gross" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
+
<td width="450px">
-
+
 
-
   <p id="text_gross" align="center"> Abstract </p>  
+
   <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
-
  <p id="text">
+
 
 +
  <div id="text">
 +
   <p id="text_gross" align="left"> Abstract </p>
 +
 
   Our Team developed a universal toolkit,  termed uniCAS, that enables customizable gene     
   Our Team developed a universal toolkit,  termed uniCAS, that enables customizable gene     
     regulation in mammalian cells.  Therefore, we engineered the recently discovered and highly promising   
     regulation in mammalian cells.  Therefore, we engineered the recently discovered and highly promising   
Line 72: Line 151:
       The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences.  
       The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences.  
       Our toolkit comprises not only a standardized Cas9 protein,  but also different effector domains for  
       Our toolkit comprises not only a standardized Cas9 protein,  but also different effector domains for  
-
       efficient gene activation or repression. </p>  
+
       efficient gene activation or repression.  
-
   <img id="klammer_gross" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
+
</div>  
 +
 +
   <img id="klammer_gross_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
 +
 
</td>
</td>
 +
 +
<!-- ########################################################################################
 +
 +
PROJECT
 +
 +
######################################################################################## -->
 +
 +
 +
<td>
<td>
-
   <img id="klammer_gross" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
+
 
-
   <img id="cas" src="https://static.igem.org/mediawiki/2013/9/94/Freiburg_2013_main2_Cas.png">
+
   <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
-
   <img id="activation" src="https://static.igem.org/mediawiki/2013/0/03/Freiburg_2013_main2_Activation.png">
+
    
-
   <img id="klammer_gross" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
+
<div id="text-box2">
 +
  <a href="https://2013.igem.org/Team:Freiburg/Project/1">
 +
    <p id="text_gross" align="left"> Our Project </p>
 +
  </a>
 +
  <div>
 +
 
 +
<ul style="margin-left:40px;">
 +
 
 +
<li style="margin-top:-30px;">
 +
<span> Activation </span>
 +
<img  src="https://static.igem.org/mediawiki/2013/0/03/Freiburg_2013_main2_Activation.png">
 +
</li>
 +
 
 +
<li style="margin-left: -40px;">
 +
<span> Repression </span>
 +
<img  src="https://static.igem.org/mediawiki/2013/e/e7/Repression_freiburg_13.png">
 +
</li>
 +
    
 +
<li style="margin-bottom:-30px;">
 +
<span> Epigenetics </span>
 +
<img src="https://static.igem.org/mediawiki/2013/3/31/Freiburg_2013_main2_Epigenetic.png">
 +
</li>
 +
</ul>
 +
 
 +
 
 +
 
 +
<ul style="margin-left:130px;">
 +
 
 +
<li style="margin-top: -30px;">
 +
  <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
 +
<span> Multiple targeting </span>
 +
</li>
 +
 
 +
<li style="margin-left: 40px;">
 +
  <img src="https://static.igem.org/mediawiki/2013/e/ef/UniBAss_freiburg_13.png">
 +
<span> uniBAss</span>
 +
</lI>
 +
 
 +
<li style="margin-bottom:-30px;">
 +
  <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png">
 +
<span> Light switch </span>
 +
</li>
 +
</ul>
 +
 
 +
</div>
 +
</div>
 +
 
 +
   <img id="klammer_gross_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
 +
 
</td>
</td>
</tr>
</tr>
</table>
</table>
 +
 +
 +
<!-- ########################################################################################
 +
 +
TABLE UNTEN
 +
 +
######################################################################################## -->
 +
 +
 +
 +
<table id="table_viereck">
 +
 +
<!-- ########################################################################################
 +
 +
HIGHLIGHTS
 +
 +
######################################################################################## -->
 +
 +
 +
<tr>
 +
  <td width="300px">
 +
  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box3-4">
 +
      <p id="text_gross" align="left"> Highlights </p>
 +
  <!-- Our Team developed...  -->
 +
  <div style="z-index:2;">
 +
  <img src="https://static.igem.org/mediawiki/2013/d/d6/Freiburg_2013_main2_Highlights.png" height="50" style="position:relative; top:-30px; left:50px;"> </div>
 +
  </div>
 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
 +
  </td>
 +
 +
 +
<!-- ########################################################################################
 +
 +
TOOLKIT
 +
 +
######################################################################################## -->
 +
 +
 +
  <td width="300px">
 +
  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box3-4">
 +
  <a href="https://2013.igem.org/Team:Freiburg/Project/toolkit">
 +
      <p id="text_gross" align="left"> Toolkit </p>
 +
  </a>
 +
  Use our Toolkit to regulate your genes!
 +
  <!-- <img src="https://static.igem.org/mediawiki/2013/d/d9/Freiburg_2013_main2_Toolkit.png" height="80" style="position:relative; top:-100px; left:50px;"> -->
 +
  </div>
 +
  </div>
 +
 
 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
 +
  </td>
 +
 +
 +
<!-- ########################################################################################
 +
 +
HUMAN PRACTICE
 +
 +
######################################################################################## -->
 +
 +
 +
  <td width="250px">
 +
  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box5-6">
 +
  <a href="https://2013.igem.org/Team:Freiburg/HumanPractice">
 +
      <p id="text_gross" align="left"> HP </p>
 +
  </a>
 +
  Our Team developed... </div>
 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
 +
  </td>
 +
 +
<!-- ########################################################################################
 +
 +
TEAM
 +
 +
######################################################################################## -->
 +
 +
 +
  <td width="250px">
 +
  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box5-6">
 +
  <a href="https://2013.igem.org/Team:Freiburg/Team2">
 +
      <p id="text_gross" align="left"> Team </p>
 +
  </a>
 +
  Our Team developed... </div>
 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
 +
</div>
</div>
</body>
</body>
</html>
</html>

Latest revision as of 16:38, 27 September 2013


Abstract

Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently discovered and highly promising CRISPR/Cas9 system. The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences. Our toolkit comprises not only a standardized Cas9 protein, but also different effector domains for efficient gene activation or repression.

Our Project

  • Activation
  • Repression
  • Epigenetics
  • Multiple targeting
  • uniBAss
  • Light switch

Highlights

Toolkit

Use our Toolkit to regulate your genes!

HP

Our Team developed...

Team

Our Team developed...

Retrieved from "http://2013.igem.org/Team:Freiburg/main2"