Template:Team:Uppsala/JS/notebook

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MikaelS (Talk | contribs)

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Line 1:

<html>

-

var month = ~~new Date().getMonth()~~;

+

var month = 8;

var year = new Date().getFullYear();

var cal_table = '';

var eventString;

var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);

-

var days_with_dirys = new Array('t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717'

+

var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');

-

,'t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385'

+

var inputs = 106;

-

,'t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820'

+

-

,'t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399'

+

-

,'t2013910','t2013911','t2013912','~~t2013913~~','~~t2013914~~','~~t2013915~~','~~t2013919~~');

+

-

var inputs = 93;

+

Date.prototype.getWeek = function()

Line 151:

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eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

document.getElementById('file_holder').innerHTML = eventString;

+

document.getElementById('calendar').style.marginBottom="350px";

}

Line 188:

Line 185:

eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

}

+

document.getElementById('calendar').style.marginBottom="350px";

document.getElementById('file_holder').innerHTML = eventString;

eventString = '';

Line 211:

Line 209:

if(id == 'd2013630')

{

-

ds = '<h2>Sunday 2013-06-30</h2><~~p><strong~~>Name of participants:</~~strong~~> Magnus B, Alexander W, Nils A, Mikael ~~Strandgren (MS)~~, Anders ~~Edlund (AE)~~ '+

+

ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1>Name of participants: Magnus B, Alexander W, Nils A, Mikael S, Anders E <h2>Ongoing constructs:</h2>E. coli: 75. pEL3A15 - CrtB 25. pSB1C3-CrtY 27. pEL3K16-red 51.pEL3C18-red 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 L. reuteri: Lb1. CF48-pLR581 Lb2. 1063-pLUL631 Lb3. DSM 20016-pVs2 Lb4. 100-23-noplasmid Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP Lb8. 100-23-pGT232 Lb12. DSM 20016-noplasmid Lb14. DSM 20016-pLUL631 L. plantarum: Lb5. 256-rifR-pAMβ1 L. lactis: Lb13. MG1363-pJP059 Lb15. unknown-pGus Lb16. MG1363-noplasmid Lb10. 256-noplasmid Lb11. 36E-noplasmid Lb9. JH2-2 pAMβ1 <h2>Todays work:</h2> Transformation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red 75. pEL3A15 - CrtB Digest 27. pEL3K16-red 51.pEL3C18-red Ligation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly. O/N Screening 25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Results</h2> Re-streak Lb13. Lc. lactis MG1363 pJP059: Good growth Lb16. Lc. lactis MG1363 noplasmid: Good growth Lb15. Lc. lactis MG1363-pGUS: Good growth 19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed 75. pEL3A15 - CrtB failed </div>';

-

'<h3>Ongoing constructs:</h3>75. pEL3A15 - CrtB 25. pSB1C3-CrtY 27. pEL3K16-red '+

+

-

'51.pEL3C18-red 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF '+

+

-

'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

-

'42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 '+

+

-

'65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP '+

+

-

'70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP '+

+

-

'74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 '+

+

-

'L. reuteri: Lb1. CF48-pLR581 Lb2. 1063-pLUL631 Lb3. DSM 20016-pVs2 '+

+

-

'Lb4. 100-23-noplasmid Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP Lb8. 100-23-pGT232 '+

+

-

'Lb12. DSM 20016-noplasmid Lb14. DSM 20016-pLUL631 <~~strong~~>L. plantarum:</~~strong~~> Lb5. 256-rifR-pAMβ1 '+

+

-

'L. lactis: Lb13. MG1363-pJP059 Lb15. unknown-pGus Lb16. MG1363-noplasmid '+

+

-

'Lb10. 256-noplasmid Lb11. 36E-noplasmid Lb9. JH2-2 pAMβ1 <h3>Todays work</h3> <h3>Transformation</h3>'+

+

-

'61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 '+

+

-

'67. pSB1C3-Cp44 77. pSB4C15-red '+

+

-

'75. pEL3A15 - CrtB <h3>Digest</h3>27. pEL3K16-red 51.pEL3C18-red '+

+

-

'<h3>Ligation</h3>61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

-

'66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly. <h3>O/N Screening</h3>'+

+

-

'25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF '+

+

-

'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h3>Results</h3> Re-streak '+

+

-

'Lb13. Lc. lactis MG1363 pJP059: Good growth Lb16. Lc. lactis MG1363 noplasmid: Good growth Lb15. Lc. lactis MG1363-pGUS: Good growth 19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed '+

+

-

'20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed 75. pEL3A15 - CrtB failed ';

+

}

else if(id == 'd2013629')

{

-

ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br~~><p~~>Name of participants: Magnus B, Alexander W, Nils~~'+ '~~A, Anders E, Mikael S '+

+

ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1> Name of participants: Magnus B, Alexander W, Nils A, Anders E, Mikael S <h2>Ongoing constructs:</h2>L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli (strain D5α): 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24.B0034-CrtI 38. pEL3A16-red 42. pSB4S15-red 57.B0034-Idi 75. pEL3A15-CrtB 76. pEL3K16-CrtI 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Todays work</h2>PCR purification: CP1 CP8 CP11 CP29 CP30 CP41 Cp44 Digestion: 27. pEL3K16-red 51.pEL3C18-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR) Ligation: 24.3 B0034-CrtI -> 27. pEL3K16-red 23.2 pSB1C3-CrtB -> 38. pEL3A16-red Transformation: 75. pEL3A15 – CrtB* 76. pEL3K16 – CrtI* *Cancelled, wrong solution was used -> redo tomorrow. Re-streak: 1.2 pSB1C3-red (from frozen stock) 7. pSB3K3 (”) 60. PK401 dam Plasmid preparation: 42. pSB4S15-red O/N Screening PCR: 25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF (19,20,21(with DMSO dilution series(7,9,12)) PCR (screening?): 57.B0034-Idi IspA Gel electrophoresis: 57.B0034-Idi IspA 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 38. pEL3A16-red (digest) 23.1 pSB1C3-CrtB (digest) 23.2 pSB1C3-CrtB (digest) 27. pEL3K16-red (digest) 24.3 B0034-CrtI (digest) 24.4 B0034-CrtI (digest) <h2>Results</h2>Today: Plasmid preparation: 42.1 pSB4S15-red klon 1: 27.4 ng/µL 42.2 pSB4S15-red klon 2: 21.8 ng/µL 42.3 pSB4S15-red klon 3: 24.3 ng/µL 42.4 pSB4S15-red klon 4: 25.3 ng/µL Gel electrophoresis: 57.B0034-Idi: still not good after three attempts IspA: eventually good 38. pEL3A16-red (digest): failed, redo subcloning tomorrow 23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 27. pEL3K16-red (digest): succeded 24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow 24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow Transformation: 75. pEL3A15 - CrtB failed PCR purification (of promotors):* CP1: 40 ng/µl CP8: 41,3 ng/µl CP11: 46,7 ng/µl CP29: 40 ng/µl CP30: 58 ng/µl CP41: 41,9 ng/µl Cp44: 42,3 ng/µl *PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor constructs (not 77) from annealing of R and F (without PCR-cleaning). Previous day: ON screening: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions </div>';

-

'<h2>Ongoing constructs:</h2>'+

+

-

'L. reuteri: '+

+

-

'1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP '+

+

-

'8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 '+

+

-

'10. 256-noplasmid 11. 36E-noplasmid '+

+

-

'E. faecalis: '+

+

-

'9. JH2-2 pAMβ1 '+

+

-

'L. lactis: '+

+

-

'13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid '+

+

-

'E.coli (strain D5α): '+

+

-

'19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

-

'23. pSB1C3-CrtB 24.B0034-CrtI 38. pEL3A16-red 42. pSB4S15-red 57.B0034-Idi 75. pEL3A15-CrtB '+

+

-

'76. pEL3K16-CrtI 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

-

'66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP '+

+

-

'71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 '+

+

-

'<h2>Todays work</h2>'+

+

-

'PCR purification: '+

+

-

'CP1 CP8 CP11 CP29 CP30 CP41 Cp44 '+

+

-

'Digestion: '+

+

-

'27. pEL3K16-red 51.pEL3C18-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

-

'66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP '+

+

-

'71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP '+

+

-

'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR) '+

+

-

'Ligation: '+

+

-

'24.3 B0034-CrtI -> 27. pEL3K16-red 23.2 pSB1C3-CrtB -> 38. pEL3A16-red '+

+

-

'Transformation: '+

+

-

'75. pEL3A15 – CrtB* 76. pEL3K16 – CrtI* *Cancelled, wrong solution was used -> redo tomorrow. '+

+

-

'Re-streak: '+

+

-

'1.2 pSB1C3-red (from frozen stock) 7. pSB3K3 (”) 60. PK401 dam '+

+

-

'Plasmid preparation: '+

+

-

'42. pSB4S15-red '+

+

-

'O/N Screening PCR: '+

+

-

'25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

-

'(19,20,21(with DMSO dilution series(7,9,12)) '+

+

-

'PCR (screening?): '+

+

-

'57.B0034-Idi IspA '+

+

-

'Gel electrophoresis: '+

+

-

'57.B0034-Idi IspA 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF '+

+

-

'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 38. pEL3A16-red (digest) 23.1 pSB1C3-CrtB (digest) 23.2 pSB1C3-CrtB (digest) '+

+

-

'27. pEL3K16-red (digest) 24.3 B0034-CrtI (digest) 24.4 B0034-CrtI (digest) '+

+

-

'<h2>Results</h2>'+

+

-

'Today: '+

+

-

'Plasmid preparation: '+

+

-

'42.1 pSB4S15-red klon 1: 27.4 ng/µL 42.2 pSB4S15-red klon 2: 21.8 ng/µL 42.3 pSB4S15-red klon 3: 24.3 ng/µL '+

+

-

'42.4 pSB4S15-red klon 4: 25.3 ng/µL '+

+

-

'Gel electrophoresis: '+

+

-

'57.B0034-Idi: still not good after three attempts IspA: eventually good 38. pEL3A16-red (digest): failed, redo subcloning tomorrow '+

+

-

'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow '+

+

-

'27. pEL3K16-red (digest): succeded 24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow '+

+

-

'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow '+

+

-

'Transformation: '+

+

-

'75. pEL3A15 - CrtB failed '+

+

-

'PCR purification (of promotors):* '+

+

-

'CP1: 40 ng/µl CP8: 41,3 ng/µl CP11: 46,7 ng/µl CP29: 40 ng/µl CP30: 58 ng/µl CP41: 41,9 ng/µl Cp44: 42,3 ng/µl '+

+

-

'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+

+

-

'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+

+

-

'constructs (not 77) from annealing of R and F (without PCR-cleaning). '+

+

-

'Previous day: '+

+

-

'ON screening: '+

+

-

'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions '+

+

-

'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions <br~~></p~~></div>';

+

}

else if(id == 'd2013628')

{

-

ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1~~><p~~>Name of participants: '+

+

ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1>Name of participants: Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W <h2>Ongoing constructs:</h2>L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57.B0034-Idi ispA <h2>Todays work</h2>Frozen stock: Lb13. Lc. lactis MG1363 pJP059 clone 2 Lb15. Lc. lactis unknown pGus clone 2 Lb16. Lc. lactis MG1363 noplasmid clone 2 Digest: 25.pSB1C3-CrtY Transformation: 24.pSB1C3-CrtI 23.pSB1C3-CrtB Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)” Lb15. Lc. lactis MG1363 pGus clone 2 “I, II, III(2x1.5 ml culture)” 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 Re-streaks: Lb3. reuteri DSM20016 pVS2 Lb8. reuteri 100-23 pGT232* Lb11. plantarum 36E Lb13. Lc. lactis MG1363 pJP059 Lb16. Lc. lactis MG1363 noplasmid *from frozen stock Streaking of strains: Lb15. Lc. lactis MG1363 plasmid unknown pGus: Lb18. Lb. delbrueckii bulgaricus (from freeze dried yoghurt culture) Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) O/N of restreak from Erik Gullberg: 42. pSB4S15-red, clones 1 and 2 PCR: 57.B0034-Idi* ispA* 19.1** 20.1** 21.1** * Amplified from D5α-chromosome. ** Now with DMSO Gel electrophoresis: 57.B0034-Idi ispA 19.1 20.1 21.1 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS O/N Screening: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 <h2>Results</h2>Previous day: Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4) → Store in freezer, try again with new protocol Transformation: Lb10. plantarum 256 pLUL364: No growth Lb11. plantarum 36E pVS2: No growth Lb12. Lc. lactis DSM20016-pVS2: No growth → Try again later with new competent cells Wednesday 26/6: Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with antibiotics Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today Today: Plasmid preparation: Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “II”: 7,5 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl → Mark and store in freezer. Gel electrophoresis: 57.B0034-Idi: hard do decipher results, redo tomorrow ispA: (“) 19.1: failed 20.1: failed 21.1: failed 43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28) 44. pSB1C3-B0034-His-TAL: failed (“) 45. pSB1C3-B0034-4CL: failed (“) 46. pSB1C3-B0034-His-4CL: failed (“) 47. pSB1C3-B0034-STS: failed (image 2 2013.06.28) 48. pSB1C3-B0034-His-STS: failed (“) <h2>Other experiments</h2>THMS buffer preparation Aliquoting of sigma water Preparation of antibiotic-free LB-agar plates Dilution of promotor-oligonucleotides from stock to 5 µM: CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R CP29_F CP29_R CP41_F CP41_R Dilution of promotor oligonucleotide from pellet to 100 µM: usp45_mut_F Phosphorylation of promotor-oligonucleotides: CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R CP29_F CP29_R CP41_F CP41_R</div>';

-

'Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M.,'+

+

-

' Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W '+

+

-

'<h2>Ongoing constructs:</h2>'+

+

-

'L. reuteri: '+

+

-

'1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid '+

+

-

'6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid '+

+

-

'14. DSM 20016-pLUL631(B?) '+

+

-

'L. plantarum: '+

+

-

'5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 '+

+

-

'L. lactis: '+

+

-

'13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid '+

+

-

'E.coli: '+

+

-

'3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 15. pSB4K5-RBS-TAL'+

+

-

'-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034'+

+

-

'-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034'+

+

-

'-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034'+

+

-

'-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034'+

+

-

'-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3'+

+

-

'-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3'+

+

-

'-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. '+

+

-

'pSB1C3-B0034-His-STS 57.B0034-Idi ispA '+

+

-

'<h2>Todays work</h2>'+

+

-

'Frozen stock: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059 clone 2 Lb15. Lc. lactis unknown pGus clone 2 Lb16. Lc. lactis'+

+

-

' MG1363 noplasmid clone 2 '+

+

-

'Digest: '+

+

-

'25.pSB1C3-CrtY '+

+

-

'Transformation: '+

+

-

'24.pSB1C3-CrtI 23.pSB1C3-CrtB '+

+

-

'Plasmid preparation: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)” Lb15. Lc. lactis MG1363 '+

+

-

'pGus clone 2 “I, II, III(2x1.5 ml culture)” 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 '+

+

-

'7. pSB3K3-red 11. pSB4A15 '+

+

-

'Re-streaks: '+

+

-

'Lb3. reuteri DSM20016 pVS2 Lb8. reuteri 100-23 pGT232* Lb11. plantarum 36E Lb13. Lc. lactis'+

+

-

' MG1363 pJP059 Lb16. Lc. lactis MG1363 noplasmid *from frozen stock '+

+

-

'Streaking of strains: '+

+

-

'Lb15. Lc. lactis MG1363 plasmid unknown pGus: Lb18. Lb. delbrueckii bulgaricus (from freeze dried'+

+

-

' yoghurt culture) Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) '+

+

-

'O/N of restreak from Erik Gullberg: '+

+

-

'42. pSB4S15-red, clones 1 and 2 '+

+

-

'PCR: '+

+

-

'57.B0034-Idi* ispA* 19.1** 20.1** 21.1** * Amplified from D5α-chromosome. '+

+

-

'** Now with DMSO '+

+

-

'Gel electrophoresis: '+

+

-

'57.B0034-Idi ispA 19.1 20.1 21.1 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL '+

+

-

'45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-'+

+

-

'STS '+

+

-

'O/N Screening: '+

+

-

'19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker'+

+

-

'-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC'+

+

-

'-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker'+

+

-

'-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-'+

+

-

'CrtB-B0034-CrtI-Linker-Zif268 '+

+

-

'<h2>Results</h2>'+

+

-

'Previous day: '+

+

-

'Plasmid preparation: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4) → Store in freezer, try again '+

+

-

'with new protocol '+

+

-

'Transformation: '+

+

-

'Lb10. plantarum 256 pLUL364: No growth Lb11. plantarum 36E pVS2: No growth Lb12. Lc. lactis '+

+

-

'DSM20016-pVS2: No growth → Try again later with new competent cells '+

+

-

'Wednesday 26/6: '+

+

-

'Streaking of strains: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today '+

+

-

'Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with '+

+

-

'antibiotics Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations '+

+

-

'(1x, 5x & 10x) → Re-streak today '+

+

-

'Today: '+

+

-

'Plasmid preparation: '+

+

-

'Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 '+

+

-

'“II”: 7,5 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl '+

+

-

'Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl'+

+

-

' Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl → Mark and store in freezer. '+

+

-

'Gel electrophoresis: '+

+

-

'57.B0034-Idi: hard do decipher results, redo tomorrow ispA: (“) 19.1: failed 20.1: failed'+

+

-

' 21.1: failed 43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28) 44. pSB1C3-B0034-His-TAL: '+

+

-

'failed (“) 45. pSB1C3-B0034-4CL: failed (“) 46. pSB1C3-B0034-His-4CL: failed (“) 47. '+

+

-

'pSB1C3-B0034-STS: failed (image 2 2013.06.28) 48. pSB1C3-B0034-His-STS: failed (“) '+

+

-

'<h2>Other experiments</h2>'+

+

-

'THMS buffer preparation '+

+

-

'Aliquoting of sigma water '+

+

-

'Preparation of antibiotic-free LB-agar plates '+

+

-

'Dilution of promotor-oligonucleotides from stock to 5 µM: '+

+

-

'CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R '+

+

-

'CP29_F CP29_R CP41_F CP41_R '+

+

-

'Dilution of promotor oligonucleotide from pellet to 100 µM: '+

+

-

'usp45_mut_F '+

+

-

'Phosphorylation of promotor-oligonucleotides: '+

+

-

'CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R '+

+

-

'CP29_F CP29_R CP41_F CP41_R~~ ~~</div>';

+

}

-

else if(id == 'd201373')

+

else if(id == 'd2013627')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-06-27</h1> Name of participants: Marcus H, Lovisa P, Emil M, Peter C, Ken B-A, Karl H, Kristoffer L, Christoffer A, Mikael S, Jens I, Nafisa B, Stephanie H, Alona N, Viktor T, Viktor B, Nils, Alexander, Thorsten, Pontus, Magnus, Malin <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS <h2>Todays work</h2> Transformation: Lb11. plantarum 36E pVS2 Lb10. plantarum 256 pLUL634 Lb12. plantarum DSM20016 pVS2 Assembly: 23.Crt.B + pEL3A15 24.Crt.I + pEL3K16 Frozen stock: Lb9. faecalis JH2-2 pAMβ1 Lb13. Lc. lactis MG1363 pJP059 clone 1 Lb15. Lc. lactis MG1363 pGus clone 1 Lb16. Lc. lactis MG1363 no plasmid clone 1 Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 1 Lb15. Lc. lactis MG1363 pGus clone 1 Lb9. faecalis JH2-2 pAMβ1 Over night: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19. (digest from previous day) 20. (“) 21. (“) PCR amplification of construct: miraculin 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 25. pSB1C3-CrtY 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS O/N screening PCR: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 25.pSB1C3-CrtY <h2>Results</h2> Previous day: O/N: Lb13. Lc. lactis MG1363 pJP059 clone 2: Visual pellet, foggy medium; nc ok! * Lb15. Lc. lactis MG1363 pGus clone 2: Visual pellet, foggy medium; nc ok! * Lb16. Lc. lactis MG1363 no plasmid clone 2: Visual pellet, foggy medium; nc ok!* Lb13. Lc. lactis MG1363 pJP059 clone 1: Visual pellet, slightly foggy medium; nc ok! ** Lb15. Lc. lactis MG1363 pGus clone 1: Visual pellet, slightly foggy medium; nc ok! ** Lb16. Lc. lactis MG1363 no plasmid clone 1: Visual pellet, slightly foggy medium; nc ok! ** * Optimized conditions = 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. → Let grow for one more day ** → Frozen stock and plasmid prep. Transformation: 42. pSB4S15-red: Many small white colonies, contamination with cocci? → Discard, get clones from Erik G. Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: Some growth. Lb15. Lc. lactis MG1363 pGus: Hard to see without opening lock. Lb16. Lc. lactis MG1363 no plasmid: Hard to see without opening lock. → let grow one more day. Today: Plasmid preparation: Lb9. faecalis JH2-2 pAMβ1 clone 1: 10.1 ng/µl Lb9. faecalis JH2-2 pAMβ1 clone 2: 24.7 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 1: 4.9 ng/µl (run 1) Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.0 ng/µl (run 2) Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.1 ng/µl (run 3) Lb15. Lc. lactis MG1363 pGus clone 1: 61 ng/µl (run 1) → Measure again tomorrow Gel electrophoresis: Miraculin: fail, problems with primers, probably due to problematic secondary structure. 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: failed, ladder too, probably problem with gel. Image 1 (2013.06.27) 19. (digest from previous day): Success 20. (“): Success 21. (“): Success 19. (PCR): Fail -> redo with lower TM 20. (“): Fail -> redo with lower TM 21. (“): Fail -> redo with lower TM 25. pSB1C3-CrtY: Good results <h2>Other experiments</h2> Preparation of THMS buffer</div>'

+

}

+

else if(id == 'd2013626')

+

{

+

ds = '<div id="dairy-text"><h2>Wednesday 2013-06-26</h2> Name of participants: Christoffer A, Stephanie H, Alona N, Anders E, Mikael S, Anton B, Viktor B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Theodor L, Magnus B., Malin B., Thorsteinn O., Nils A., Alexander W., Niclas S., Pontus D., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 25.pSB1C3-CrtY 19.psB4C5-CrtE~O-Z / w. linker, ZF & RBS 20.psB4C5-CrtE~Z / w. linker, ZF & RBS 21.psB4C5-CrtE~I / w. linker, ZF & RBS 42. D5α with plasmid pSB4S15-red <h2>Todays work</h2> Competent cells preparation: Lb11. plantarum 36E no plasmid Lb10. plantarum 256 no plasmid Lb12. reuteri DSM 20016 no plasmid Transformation: plantarum 36E pAMB1 plantarum 256 pAMB1 Lb3. reuteri DSM 20016 pVS2 25.pSB1C3-CrtY 42. pSB4S15-red O/N: Lb5.2 plantarum 256 pAMβ1* Lb8.2 reuteri 100-23 pGT232* Lb13. Lc. lactis MG1363 pJP059** Lb15. Lc. lactis MG1363 pGus** Lb16. Lc. lactis MG1363 no plasmid** *Two tubes of 8.2 were prepared. It has grown rather poorly to not at all before. If it continues to grow poorly we may have to redo O/N from a plate rather than from our frozen stock. ** Optimized conditions → 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. Digestion: 19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS 19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS 20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS 20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS 21.1.psB4C5-CrtE~I / w. linker, ZF & RBS 21.2.psB4C5-CrtE~I / w. linker, ZF & RBS Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT PCR amplification of construct: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (third try, using same concentration of template as second try but lower annealing temperature ) 19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS 19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS 20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS 20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS 21.1.psB4C5-CrtE~I / w. linker, ZF & RBS 21.2.psB4C5-CrtE~I / w. linker, ZF & RBS <h2>Results</h2> Previous day: O/N: Lb13. Lc. lactis MG1363 pJP059: Growing, visual pellet.* Lb15. Lc. lactis MG1363 pGus: Growing, visual pellet.* Lb16. Lc. lactis MG1363 no plasmid: Growing, visual pellet.* *Weak growth in the morning, better in the afternoon. → Let grow for one more day + new O/N with optimized conditions. Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: No visual growth → Let grow for one more day. Lb15. Lc. lactis MG1363 pGus: No visual growth → --”-- Lb16. Lc. lactis MG1363 no plasmid: No visual growth → --”— Today: Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: Failed, possibly nonspecific primer binding, not enough template DNA. Image 1 (2013.06.26) Transformation: 25.pSB1C3-CrtY: failed D5alpha competent cells were competent <h2>Other experiments</h2> Preparation of cold electroporation buffer for competent cells Preparation of 50% glycerol stock: 500 ml 50% glycerol stock was prepared from 86-88% glycerol.</div>'

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}

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else if(id == 'd2013625')

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{

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ds = '<div id="dairy-text"><h1>Tuesday 2013-06-25</h1> Name of participants: Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Theodor L, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Gel electrophoresis: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m PCR amplification of construct: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (second try, using smaller concentration of template and higher annealing temperature ) Lb13. Lc. lactis MG1363 pJP059 Competent cells preparation: Lb11. plantarum 36E no plasmid Lb10. plantarum 256 noplasmid Lb12. reuteri DSM 20016 no plasmid Plasmid preparation: Lb6.1 reuteri DSM 20016 pLUL634TsA8 Lb6.2 reuteri DSM 20016 pLUL634TsA8 Lb6.3 reuteri DSM 20016 pLUL634TsA8 Lb6.4 reuteri DSM 20016 pLUL634TsA8 * Lb7.2 reuteri DSM 20016 pGFP Lb7.3 reuteri DSM 20016 pGFP Lb7.4 reuteri DSM 20016 pGFP * Lb14.1 reuteri DSM 20016 pLUL631 Lb14.2 reuteri DSM 20016 pLUL631 Lb14.3 reuteri DSM 20016 pLUL631 Lb14.4 reuteri DSM 20016 pLUL631 * * 6.4, 7.4 and 14.4 were treated in a different way than the regular plasmid prep protocol for L. reuteri. Instead of treating them with LYSIS-buffer and lysozyme they were treated with resuspension solution and lysis solution from the plasmid prep kit and allowed to lyse for ~30 minutes. The rest were treated as usual, but with 1 ml of 10 mg/ml lysozyme instead of 2 ml. O/N: Lb13. Lc. lactis MG1363 pJP059 Lb15. Lc. lactis MG1363 pGus Lb16. Lc. lactis MG1363 no plasmid Streaking of strains: Lb13. Lc. lactis MG1363 pJP059 Lb15. Lc. lactis MG1363 pGus Lb16. Lc. lactis MG1363 no plasmid PCR amplification: Lb13. Lc. lactis MG1363 pJP059* *Another attempt at colony PCR with different concentrations of lysozyme and buffer. <h2>Results</h2> Gel electrophoresis: 15. failed. Possibly nonspecific primer binding, too much template DNA. Image 1 (2013.06.25) Lb13. Lc. lactis MG1363 pJP059: Only short bands, probably primer dimers because lysing failed. → Try again with a plasmid prep instead of culture. Competant cells: Wrong volume was added. → Try again tomorrow with the right volume. Plasmid preparation: * Lb6.1 reuteri DSM 20016 pLUL634 TsA8: 203.7 ng/µl Lb6.2 reuteri DSM 20016 pLUL634 TsA8: 203.2 ng/µl Lb6.3 reuteri DSM 20016 pLUL634 TsA8: 154.8 ng/µl Lb6.4 reuteri DSM 20016 pLUL634 TsA8: 39.5 ng/µl Lb7.2 reuteri DSM 20016 pGFP: 100.5 ng/µl Lb7.3 reuteri DSM 20016 pGFP: 141.2 ng/µl Lb7.4 reuteri DSM 20016 pGFP: 8.7 ng/µl Lb14.1 reuteri DSM 20016 pLUL631: 162.7 ng/µl Lb14.2 reuteri DSM 20016 pLUL631: 85.2 ng/µl Lb14.3 reuteri DSM 20016 pLUL631: 49.8 ng/µl Lb14.4 reuteri DSM 20016 pLUL631: 7.7 ng/µl *The alternative way of lysing the cells proved to be quite ineffective so we will continue to use our standard protocol. The earlier plasmid preparations of the above “constructs”, that had far poorer concentrations, were replaced with these better ones. <h2>Other experiments</h2> Preparation of MRS broth Making plates for Lc. lactis cultivation (from M17-broth) Preparation of homemade yogurt from dried bacteria and from commercial yogurt with active bacteria culture</div>'

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}

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else if(id == 'd2013624')

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{

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ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1> Name of participants: Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis 13. MG1363-pJP059 E. coli: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Plasmid preparation: Lb8. reuteri 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 Digestion of construct: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m O/N (from frozen stock): Lb2. 1063 pLUL631 Lb6. DSM 20016 pLUL63TsA8 Lb7. DSM 20016 pGFP Lb8. 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 PCR: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT* Lb13. Lc. lactis MG1363 pJP059* *Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: 1.1. pSB1C3-red 18.2. pSB1C3-CHS_m 40. pEL3A17-red <h2>Results</h2> Plasmid preparation: Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today. <h2>Other experiments</h2> Primer stock preparation Dilution of sequencing primers: 3 tubes of 5 µM VF2-primer, 200 uL 3 tubes 5 µM VR-primer, 200 µL Re-measuring concentration of earlier plasmid preps: * 1.4 CF48-pLR581: 127.8 ng/µl 1.5 CF48-pLR581: 215.9 ng/µl 3.2 DSM 20016-pVs2: 201.2 ng/µl 3.3 DSM 20016-pVs2: 139.1 ng/µl 3.10 DSM 20016-pVs2: 183.5 ng/µl *We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind. Preparing M17-broth, 950 ml. (“) SOB (“) CCMB80 (20% glycerol, pH 6,8) Alliquoting dNTP, buffer, enzymes</div>'

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}

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else if(id == 'd2013623')

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{

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ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1> Name of participants: Hampus E, Alexander W <h2>Ongoing constructs:</h2> L. reuteri: 4. 100-23-noplasmid L. plantarum: 5. 256-rifR-pAMβ1 E. coli: 23.pCrcSB1C3-Crt-B 24.pSB1C3-Crt-I 25. pSB1C3-CrtY <h2>Todays work</h2> Glycerol stock preparation: Lb4. reuteri 100-23 no plasmid Lb5. plantarum 256 rifR-Pamβ1 23.pCrcSB1C3-Crc-B 24.pSB1C3-Crc-I Restreak: 25. pSB1C3- crtY Plasmidpreparation 23.pCrcSB1C3-Crc-B 24.pSB1C3-Crc-I <h2>Result</h2> O/N: 25.pSB1C3-crtY: One of the overnights did not grow </div>'

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}

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else if(id == 'd2013622')

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{

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ds = '<div id="dairy-text"><h1>Saturday 2013-06-22</h1> Name of participants: Nafisa B, Mikael S, Marcus H <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Glycerol stock preparation: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb8. reuteri 100-23-pGT232 Lb9. faecalis JH2-2- pAMβ1 Lb10. plantarum 256-noplasmid Lb11. plantarum 36E-noplasmid Lb12. reuteri DSM 20016-noplasmid Lb14. reuteri DSM 20016-pLUL631 (B?) O/N: Lb4. reuteri100-23-noplasmid Lb5. plantarum 256-rifR-pAMβ1 Plasmid preparation: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red Lb14. DSM 20016-pLUL631 (B?) Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP <h2>Results</h2> Plasmid preparation: L. reuteri: Lb14.1 (strain 14, clone 1) DSM 20016-pLUL631 (B?): 10.0 ng/μl Lb14.2 DSM 20016-pLUL631 (B?): 13.6 ng/μl Lb14.3 DSM 20016-pLUL631 (B?): 14.7 ng/μl Lb14.4 DSM 20016-pLUL631 (B?): 28,4 ng/μl Lb6.1 DSM 20016-pLUL63TsA8: 18.7 ng/μl Lb6.2 DSM 20016-pLUL63TsA8: 14.4 ng/μl Lb6.3 DSM 20016-pLUL63TsA8: 12.5 ng/μl Lb6.4 DSM 20016-pLUL63TsA8: 27.7 ng/μl Lb7.2 DSM 20016-pGFP: 29.9 ng/μl Lb7.3 DSM 20016-pGFP: 16.9 ng/μl Lb7.4 DSM 20016-pGFP: 10.9 ng/μl</div>'

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}

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else if(id == 'd2013621')

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{

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ds = '<div id="dairy-text"><h1>Friday 2013-06-21</h1> Name of participants: Stephanie H, Alona N, Anton B, Mikael S, Nafisa B, Anders E, Marcus H, Ken B-A, <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP <h2>Todays work</h2> Plasmid preparation*: Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 *to get more useable material and to look for different result from clones grown in a lock and a CO2-cupboard. O/N: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb14. reuteri DSM 20016-pLUL631(B?) Lb12. reuteri DSM 20016-noplasmid Lb8. reuteri 100-23-pGT232 Lb10. plantarum 256-noplasmid Lb11. plantarum 36E-noplasmid Lb9. faecalis JH2-2 pAMβ1 *- *from the plate without erythromycin. Plasmid preparation: 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP <h2>Results</h2> Previous day: Re-streak: Lb4. reuteri 100-23-noplasmid: No growth. → Grow on non-antibiotic plates Today: Plasmid preparation: Lb1.2 (strain 1, clone 2) reuteri CF48-pLR 581: Non-usable concentration * Lb1.4 reuteri CF48-pLR 581: 161.8 ng/µl * Lb3.8 reuteri DSM 20016 pVS2: 148.5 ng/µl * Lb3.10 reuteri DSM 20016 pVS2: Non-usable concentration * *there is seemingly no correlation between growth in lock vs. CO2-cupboard and plasmid concentration. This seems strange though. Cupboard is not supposed to create an anaerobic environment → do not use cupboard <h2>Other experiments</h2> SET buffer preparation: Lysis buffer preparation: </div>'

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}

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else if(id == 'd2013620')

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{

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ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S, Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24. pSB1C3-CrtI 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work:</h2> Glycerol stock preparation: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Plasmid preparation: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 26. pEL3K15-red 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 O/N: Lb2. reuteri 1063-pLUL631 Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 Streaking of new strains from Stefan Roos: Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb12. reuteri DSM 20016-noplasmid * Lb14. reuteri DSM 20016-pLUL631(B?) Lb4. reuteri 100-23-noplasmid Lb8. reuteri 100-23-pGT232 * Lb5. plantarum 256-rifR-pAMβ1 Lb10. plantarum 256-noplasmid * Lb11. plantarum 36E-noplasmid * Lb9. faecalis JH2-2 pAMβ1 ** *both on burned broth without glucose and CaCl2 and regular one. **re-streaked on erythromycin and on antibiotic-free plates. Transformation: 23. pSB1C3-CrtB 24. pSB1C3-CrtI Restreaks: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red <h2>Results</h2> Previous day: Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17) Today: Plasmid preparation: 19.1: 50.5 ng/µl 19.2: 33.6 ng/µl 19.3: 45.2 ng/µl 19.4: 36.5 ng/µl 20.1: 28.9 ng/µl 20.2: 29.6 ng/µl 20.3: 41.8 ng/µl 20.4: 35.8 ng/µl 21.1: 47.7 ng/µl 21.2: 23.2 ng/µl 21.3: 38.8 ng/µl 21.4: 48.9 ng/µl Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again. Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again. Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl <h2>Other experiments</h2> Antibiotic-free MRS-broth plates preparation: SET-buffer and Lysis-buffer preparation: MRS-broth agar preparation: Preparation of plates with and without antibiotic: </div>'

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}

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else if(id == 'd2013619')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-06-19</h1> Name of participants: Emil M, Marcus H, Ken B-A, Sabri J., Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B, Malin B, Pontus D, Magnus B, Alexander W, Thorsteinn O, Niclas S, Nils A <h2>Ongoing constructs:</h2> L. reuteri: 2. 1063-pLUL631 1. CF48-pLR581 3. DSM20016-pVs2 Lactoccocus lactis: 13. MG1363-pJP059 E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 23. pSB1C3-CrtB 24. pSB1C3-CrtI 25. pSB1C3-CrtY 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work</h2> Re-streak: 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM20016-pVs2 Spreading: Lb13. Lc. lactis MG1363-pJP059 O/N: 14. pET13b-zifz:4cl-PBSII:STS 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM20016-pVs2 Transformation: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 23. pSB1C3-CrtB 24. pSB1C3-CrtI 25. pSB1C3-CrtY <h2>Results</h2> Previous day: Spreading: Lb13. Lc. lactis MG1363-pJP059 : No growth. → Try growing with less oxygen. Transformation 1. pSB1C3-red: Successful 2. pSB3C5-red: Successful 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: Successful 7. pSB3K3-red: fail 12. pSB4K5-red: fail Restreak: 16. pSB1C3-TAL_m: Successful 17. pSB1C3-4Cl_m: Successful 18. pSB1C3-CHS_m: Successful 22. pSB1C3-B0032-BFP: Successful Today: O/N: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth Transformation: 23. pSB1C3-CrtB: fail 24. pSB1C3-CrtI: fail 25. pSB1C3-CrtY: fail <h2>Other experiments</h2> LB-agar plates CaCl2-solution</div>'

+

}

+

else if(id == 'd2013618')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-06-18</h1> Name of participants: Malin B, Pontus D, Magnus B, Alexander V, Thorsteinn O, Niclas S, Nils A, Christoffer F, Kristoffer L, Emil M, Theodor L, Marcus H, Ken B.-A, Peter C, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B <h2>Ongoing constructs:</h2> L. reuteri: 2. 1063-pLUL631 1. CF48-pLR581 3. DSM20016-pVs2 Lactoccocus lactis: 13. MG1363-pJP059 E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP <h2>Todays work:</h2> Streaking of strains from Stefan Roos: Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Lb13. Lc. lactis MG1363-pJP059 Transformation 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP Restreak 14. pET13b-zifz:4cl-PBSII:STS 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Results:</h2> Previous day Restreak: 14. pET13b-zifz:4cl-PBSII:STS: Growth Today Transformation: 1. pSB1C3-red: fail 2. pSB3C5-red: fail 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: fail 7. pSB3K3-red: fail 12. pSB4K5-red: fail 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: fail 16. pSB1C3-TAL_m: successful 17. pSB1C3-4Cl_m: successful 18. pSB1C3-CHS_m: successful 22. pSB1C3-B0032-BFP: successful Restreak: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth <h2>Other experiments:</h2> LB-agar</div>'

+

}

+

else if(id == 'd2013617')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-06-17</h1> Name of participants: Kristoffer L, Emil M, Lovisa P, Theodor L, Marcus H, Ken B-A, Peter C, Christoffer A, Anton B, Nafisa B, Stephanie H, Alona N, Anders E, Malin B., Pontus D., Alexander V., Magnus B., Nils A. Thorsteinn O., Christoffer F <h2>Ongoing constructs:</h2> E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Todays work:</h2> Transformation 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Results:</h2> Today: Transformation: 1. pSB1C3-red: fail 2. pSB3C5-red: fail 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: fail 7. pSB3K3-red: fail 12. pSB4K5-red: fail 14. pET13b-zifz:4cl-PBSII:STS: successful 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m: fail 17. pSB1C3-4Cl_m: fail 18. pSB1C3-CHS_m: fail 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: successful 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: successful 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: successful <h2>Other experiments:</h2> Mixing MRS-broth Making of EDTA solution MRS-plates preparation</div>'

+

}

+

else if(id == 'd2013613')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-06-13</h1> Name of participants: Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F. <h2>Other experiments</h2> Making competent D5α</div>'

+

}

+

else if(id == 'd2013612')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-06-12</h1> Name of participants: Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F., Pontus D <h2>Other experiments</h2> LB-agar plates</div>'

+

}

+

else if(id == 'd2013610')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-06-10</h1> Name of participants: Alexander W, Magnus B, Malin B, Thorsteinn O, Nils A, Christoffer F., Pontus D, Niclas S. <h2>Other experiments:</h2> LB CaCl2 Antibiotics</div>'

+

}

+

else if(id == 'd201373')

{

ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1> Name of participants: Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A, Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 1. pSB1C3-red 7. pSB3K3-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red L. plantarum: Lb10. 256-noplasmid Lb11. 36E-noplasmid L. reuteri: Lb12. DSM 20016-noplasmid L. lactis: Lb13. MG1363-pJP059 <h2>Todays work</h2> PCR: Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* *Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Lb13.2.2 lactis MG1363 pJP059 (plasmid)** Lb13.2.3 lactis MG1363 pJP059 (plasmid)** Lb13.3 lactis MG1363 pJP059 (colony)** Lb13.4 Lc. lactis MG1363 pJP059 (colony)** **Gel will be done tomorrow. Followed the PCR protocol with DMSO. 78. pEL3C18-CrtY* -*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time) Digest: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Ligation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 1. pSB1C3-red -as N/C, one that we use plus a digest that has worked for other groups 7. pSB3K3-red -as N/C, one that we use plus a digest that has worked for other groups 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red -*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning. Transformation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77 pSB4C15-red O/N: Lb10.1. plantarum 256 no plasmid Lb11.1. plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Restreak: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 Plasmid preparation: 7.pSB3K3 22.pSB1C3-B0032-BFP 42. pSB4S15-red Gelelectrophoresis: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Lb13.2.3 Lc. lactis MG1363-pJP059* Lb13.3 Lc. lactis MG1363-pJP059* Lb13.4 Lc. lactis MG1363-pJP059* *Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Results Gelelectrophoresis: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red -Digestion Worked failed: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* -*The gel showed nothing but primer dimer. Digest: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Yesterdays transformation: successful: 61. pSB1C3-CP1: Growth 61. pSB1C3-CP1 10x: Some growth 62. pSB1C3-CP8: Some growth 62. pSB1C3-CP8 10x: No growth 63. pSB1C3-CP11: Some growth 63. pSB1C3-CP11 10x: No growth 64. pSB1C3-CP29: Some growth 64. pSB1C3-CP29 10x: No growth 65. pSB1C3-CP30: Some growth 65. pSB1C3-CP30 10x: No growth 66. pSB1C3-CP41: Growth 66. pSB1C3-CP41 10x: Some growth 67. pSB1C3-Cp44: No growth 67. pSB1C3-Cp44 10x: No growth 68. pSB3K3-CP1-B0032-BFP: No growth 68. pSB3K3-CP1-B0032-BFP 10x: Growth 69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated 69. pSB3K3-CP8-B0032-BFP 10x: No growth 70. pSB3K3-CP11-B0032-BFP: Growth 70. pSB3K3-CP11-B0032-BFP 10x: Growth 71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies 71. pSB3K3-CP29-B0032-BFP 10x: ----- 72. pSB3K3-CP30-B0032-BFP: Growth 72. pSB3K3-CP30-B0032-BFP 10x: No growth 73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies 73. pSB3K3-CP41-B0032-BFP 10x: No growth 77.1 pSB4C15, clone 1: No growth 77.1 pSB4C15, clone 1 10x: No growth 77.2 pSB4C15, clone 2: Some growth 77.2 pSB4C15, clone 2 10x: No growth 23. pSB1C3-CrtB 24. pSB1C3-CrtI 27. pEL3K16-red 38. pEL3A15-red 51. pEL3C18-red failed: 81. pSB4S15-CP41: No growth 82. pSB4S15-CP29: No growth 83. pSB4S15-CP30: No growth → Redo ligation and transformation. Plasmid preparation: 7.3 pSB3K3 clone 3: 50.1 ng/µl 7. 4 pSB3K3 clone 4: 43.4 ng/µl 7. 5 pSB3K3 clone 5: 48.1 ng/µl 22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl 22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ 22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ 42.1 pSB4S15-red clone 1: 43.6 ng/µl 42.2 pSB4S15-red clone 2: 46.1 ng/µl <h2>Other experiments</h2> Made agarplates with chloramphenicol resistance MRS-broth preparation MRS-broth-agar preparation MRS-broth-agar plates preparation </div>';

Line 543:

Line 424:

'<h2>Other experiments</h2> - </div>';

}

+

else if(id == 'd201382')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-02</h1> Name of participants: Anton B, Christoffer A, Mikael S, Jens I, Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E, Magnus, Pontus, Sabri, Nils <h2>Ongoing constructs:</h2> E.coli: 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 108. pSBA15-J23106-B0034-IspA 109. pSBA15-K206000-B0034-IspA 110. pSBA15-K206001-B0034-IspA 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 140. pSB3T5-J23110-B0034-UGTCs2-6xHis 141. pSB3T5-J23101-B0034-UGTCs2-6xHis 142. pSB3T5-CP1-B0034-UGTCs2-6xHis 163. pEL3S17-ZCD-His-B0034-UGTCs2-His 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 177. pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178. pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183. pEL3A15-B0034-CrtZ 184. pSB1C3-B0034-CrtZ 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 191. pSB1A2-fusionGFP 193. pSB1C3-amilGFP 194. pSB1C3-B0034-amilCP 195. pSB1C3-cjBlue 196. pSB1C3-eforRed 197. pSB1C3-aeBlue 198. pSB1C3-B0034-amilGFP 199. pSb1C3-amajCFP 200. pSB1C3-B0034-asCP 202. pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203. pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 204. pEL3S17-J23110-HIS-STS 205. pSBLB4C15-CP1-B0034-HIS-TAL 206. pSB1C3-J23101-B0034-TAL 207. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His L. reuteri: 3. DSM 20016 pVS2 4. 100-23 no plasmid 12. DSM 20016 no plasmid 23. 100-23 pSBLb4C15-red 24. DSM 20016 pSBLb4C15-red 26. 100-23 pSBLbEcC2-red 27. DSM 20016 pSBLbEcC2-red 28. 100-23 pVS2 <h2>Todays work</h2> Plasmid preparation: 139.1.1 pSB3K3-J23101-B0032-BFP 139.1.2 pSB3K3-J23101-B0032-BFP 191.1 pSB1A2-fusionGFP 191.2 pSB1A2-fusionGFP Ligation: 4.pSB1A3-Red, 147.pSB1C3-B0034-Thioredoxin, 125.pSB1C3-ZCD-His-> 208.pSB1A3-B0034-Thioredoxin-ZCD-His 36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His Streaking of plasmid-free cells from frozen stock as control of C-resistance: Lb4.1 reuteri. 100-23 no plasmid Lb12.1 reuteri DSM 20016 no plasmid Restreak of construct: 171. pSB3T5-J23110-B0034-4CL-B0034-STS 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL Screening: 99. pCB1C3 - CrtO 190. pEL3K16 - CrtO 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Overnight: 99. pCB1C3 - CrtO 190. pEL3K16 - CrtO 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Gel electrophoresis: PCR of 152. pUC57-B0034-Exp-Linker-Miraculin-Linker-His to extract Miraculin with biobrick prefix and suffix <h2>Results</h2> Today: Plasmid preparation: 139.1.1 pSB3K3-J23101-B0032-BFP: 51.9 ng/µl 139.1.2 pSB3K3-J23101-B0032-BFP: 53.6 ng/µl 191.1 pSB1A2-fusionGFP: 78.9 ng/µl 191.2 pSB1A2-fusionGFP: 74.1 ng/µl</div>'

+

}

+

else if(id == 'd201383')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1> Name of participants: Lovisa P, Magnus, Pontus <h2>Other experiments</h2> Making competent cells (D5α)</div>'

+

}

+

else if(id == 'd201383')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1> Name of participants: Lovisa P, Magnus, Pontus <h2>Other experiments</h2> Making competent cells (D5α)</div>'

+

}

+

else if(id == 'd201384')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-04</h1> Name of participants: Emil M, Magnus, Pontus <h2>Ongoing constructs:</h2> 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His <h2>Todays work:</h2> Overnight: 10. D5α <h2>Characterization</h2> Detection of TAL activity through spectroscopy (day 1) Contructs tested for TAL activity: 106.5 pSB3K3-J23110-B0034-TAL 106.6 pSB3K3-J23110-B0034-TAL 137.1 pSB3K3-CP8-B0034-TAL 189.3 pSB3K3-J23101-B0034-TAL Negative controls: 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL Positive controls (p-coumaric acid added before dextraction): 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL <h2Other experiments: Competent cell test </div>'

+

}

+

else if(id == 'd201385')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-05</h1> Name of participants: Anton B, Viktor T, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn <h2>Ongoing constructs:</h2> L. reuteri: 23. 100-23 pSBLb4C15-red 25. 100-23 pSBLbC2-red 28. 100-23 pVS2 E. coli: 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His <h2>Todays work</h2> Competent cells Lb4.1 reuteri 100-23 no plasmid Lambda red transformation: Crt Transformation: reuteri 100-23 pSB1C3-red (1 tube, to see if it would work in Lb, died in electroporation) Lb23. reuteri 100-23 pSBLb4C15-red (5 tubes, 1 died in electroporation) Lb25. reuteri 100-23 pSBLbC2-red (1 tube) Lb28. reuteri 100-23 pVS2 (2 tubes, 1 died in electroporation) 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Screening: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI <h2>Characterization</h2> Detection of TAL activity through spectroscopy (day 2) Contructs tested for TAL activity: 106.5 pSB3K3-J23110-B0034-TAL 106.6 pSB3K3-J23110-B0034-TAL 137.1 pSB3K3-CP8-B0034-TAL 189.3 pSB3K3-J23101-B0034-TAL Negative controls: 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL Positive controls (p-coumaric acid added before dextraction): 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL <h2>Other experiments</h2> Casting LB-agar plates: Kanamycin, 63 plates Chloramphenicol, 61 plates Spectinomycin, 65 plates Tetracycline, 32 plates Ampicillin, 30 plates Competent cells (D5α)</div>'

+

}

+

else if(id == 'd201386')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-06</h1> Name of participants: Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Anton B, Mikael S, Viktor T <h2>Ongoing constructs:</h2> E. coli: 57.3.pSB1C3-B0034-Idi 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 6. pSB1K3-red 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 111. pSBLb4C15-red 139. pSB3K3-J23101-B0032-BFP 165. pSB1C3-RBS-tsPurle 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 194. pSB1C3-B0034-amilCP 198. pSB1C3-B0034-amilGFP L. reuteri: 23. 100-23 pSBLb4C15-red 25. 100-23 pSBLbC2-red 28. 100-23 pVS2 <h2>Todays work:</h2> Transformation: 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His Restreak: 110.pEL3S17-K206001-B0034-IspA 115. pSB3K5-J23101-B0034-His-TAL 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Digest: 6. pSB1K3-red 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 68.2 pSB3K3-CP1-B0032-BFP (EcoRI + PstI) 69.4 pSB3K3-CP8-B0032-BFP (EcoRI + PstI) 70.3 pSB3K3-CP11-B0032-BFP (EcoRI + PstI) 71.1 pSB3K3-CP29-B0032-BFP (EcoRI + PstI) 72.2 pSB3K3-CP30-B0032-BFP (EcoRI + PstI) 73.4 pSB3K3-CP41-B0032-BFP (EcoRI + PstI) 74. pSB3K3-CP44-B0032-BFP (EcoRI + PstI) 111. pSBLb4C15-red (XbaI + PstI) 165. pSB1C3-RBS-tsPurle (XbaI + PstI) 166. pSB1C3-RBS-scOrange (XbaI + PstI) 167. pSB1C3-RBS-fwYellow (XbaI + PstI) 194. pSB1C3-B0034-amilCP (XbaI + PstI) 198. pSB1C3-B0034-amilGFP (XbaI + PstI) Ligation: B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin B0034-Thioredoxin, 1. pSB1C3-red (X, P) -> 147. pSB1C3-B0034-Thioredoxin Gel: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI Screening: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI 204.(1-4) pEL3S17-J23110-B0034-HIS-STS 171(1-4) pEL3S17-J23110-B0034-4CL-B0034-STS Mutagenesis: 43.7 pSB1C3-B0034-TAL 57.3.pSB1C3-B0034-Idi -> 129.pSB1C3-Idi(mut) <v>Sequencing preparation: 139.1 pSB3K3-J23101-B0032-BFP 185.9 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 185.10 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS O/N: 204.2 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) 111.7 pSBLb4C15-red <h2>Results:</h2> Gel: 164.E.Coli Nissle-CrtY (clones from transformation plate) failed 201.E.Coli Nissle-CrtY-CrtI failed Previous day: Transformation: No visual growth. Keeping them on growth for one more day. <h2>Characterization:</h2> Detection of TAL activity through spectroscopy (day 3) Contructs tested for TAL activity: 106.5 pSB3K3-J23110-B0034-TAL 106.6 pSB3K3-J23110-B0034-TAL 137.1 pSB3K3-CP8-B0034-TAL 189.3 pSB3K3-J23101-B0034-TAL Negative controls: 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL Positive controls (p-coumaric acid added before dextraction): 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL <h2>Other Experiments:</h2> 10.D5Alpha competence test Preparation of buffers for Western Blot Casting LB-agar plates: Kanamycin, 33 plates Spectinomycin, 31 plates </div>'

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}

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else if(id == 'd201387')

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{

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ds = '<div id="dairy-text"><h1>Wednesday 2013-08-07</h1> Name of participants: - Viktor T, Anton B, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils <h2>Ongoing constructs:</h2> E. coli: 6. pSB1K3-red 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 92. J23110 111. pSBLb4C15-red 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 240. pSB1K3-J23110-RBS-tsPurple 241. pSB1K3-J23110-RBS-scOrange 242. pSB1K3-J23110-RBS-fwYellow 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple <h2>Todays work</h2> Assembly: pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 240. pSB1K3-J23110-RBS-tsPurple* 241. pSB1K3-J23110-RBS-scOrange* 242. pSB1K3-J23110-RBS-fwYellow* *Will eventually be tried in shuttle vector, but that has C-resistance, so a subcloning to K is necessary first, or a modification to change the resistance of the shuttle vector. Transformation: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 240. pSB1K3-J23110-RBS-tsPurple 241. pSB1K3-J23110-RBS-scOrange 242. pSB1K3-J23110-RBS-fwYellow 108.pEL3S17-J23106-B0034-IspA 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue Spreading: 60. PK401 dam- no plasmid 111.7 pSBLb4C15-red Restreak: 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue O/N & Screening: 110.pEL3S17-K206001-B0034-IspA 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pBAD/AraC-RBS-Luciferas (2012 years kit) 227.pBAD/AraC-RBS-Luciferas (2013 years kit) 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple Gel: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI 129.pSB1C3-Idi(mut) <h2>Results</h2> Transformation (Monday): Still no visual growth. SDS-page failed (probably due to faulty buffers, investigate the running buffer first) <h2>Characterization experiments</h2> SDS-page on constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) <h2>Other experiments</h2> Sequencing preparation: 69. pSB3K3-CP8-B0032-BFP* *since the existing sequencing data doesn’t cover the whole insert with sufficient accuracy. There may be a mutation at the end of BFP, but that is more than 800bp after the single primer. Was not sent today, not sequencing tuesday.</div>'

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}

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else if(id == 'd201388')

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{

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ds = '<div id="dairy-text"><h1>Thursday 2013-08-08</h1> Name of participants: Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Stephanie H, Mikael S, Anton B <h2>Ongoing constructs:</h2> E. coli: 60. Strain PK401 dam- no plasmid 111.7 pSBLb4C15-red 139. pSB3K3-J23101.B0032-BFP 91. BBa_J61002-J23106 92. BBa_J61002-J23110 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS <h2>Todays work:</h2> Transformation: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 no nr. pSB1c3-Thioredoxin no nr. pEL3S17-Thioredoxin pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink Re-streak: 108.pEL3S17-J23106-B0034-IspA 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue <bPlasmid prepp 226. pBAD/AraC-RBS-Luciferas (2012 years kit) 227.pBAD/AraC-RBS-Luciferas (2013 years kit) 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple O/N screening: 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His Two step Assembly 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow Overnight (screening) 1. pSB1C3-red O/N 60. Strain PK401 dam- no plasmid 111.7 pSBLb4C15-red Streak from frozen stock 3. pSB3T5-red 6. pSB1K3-red 42. pSB4S15-red 77. pSB4C15-red <h2>Results:</h2> One of the SDS-gels failed, resulting in no good gel to stain (faulty buffers, continue to search for sources of errors) The succesfull gel was prepared with control buffers. Transformation of promotors with BFP in the shuttle vector, for characterization in Lactobacillus: Some growth, only white colonies, will let them grow overnight again. <h2>Characterization experiments:</h2> Westernblot SDS-page on constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) Electro transfer over night Staining of control gel Detection of TAL activity through spectroscopy (day 4)</div>'

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else if(id == 'd201389')

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{

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ds = '<div id="dairy-text"><h1>Friday 2013-08-09</h1> Name of participants: Stephanie H, Mikael S, Anton B, Anders E, Magnus, Pontus, Sabri, Malin, Alexander, Nils, Christoffer <h2>Ongoing constructs:</h2> 60. PK401 dam- no plasmid 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP 111.7 pSBLb4C15-red 91. BBa_J61002-J23106 92. BBa_J61002-J23110 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 244. pSB1C3-Promoter-RBS-Bleomysin 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow <h2>Todays work</h2> Streak from frozen stock: 60. PK401 dam- no plasmid 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP O/N: 60.3 PK401 dam- no plasmid* *The O/N made on thursday used MRS, so it had to be remade from restreaks. Plasmid preparation: 111.7 pSBLb4C15-red 220.pSB3K3-J23110-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Restreak: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His no nr. pEL3S17-Thioredoxin Amplification PCR 134. B0034-CBP 135. B0034-CSP 254. Gus 257. p-pem 258. pem Mutagenesis 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 O/N Screening: 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 221. pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 201. E.Coli. Nissle - CrtY-CrtI 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue Send to Linköping, as plasmid prepp and agar stabb 226.pSB1C3-pBAD/AraC-RBS-Luciferas (2012 years kit) 227.pSB1C3-pBAD/AraC-RBS-Luciferas (2013 years kit) Transformation 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink <h2>Results</h2> Plasmid preparation: 111.7 pSBLb4C15-red: 30,6 ng/µl O/N: 221 failed, redo. O/N Screening: 108.pEL3S17-J23106-B0034-IspA - Screening gel fail 109.pEL3S17-K206000-B0034-IspA - Screening gel fail 142.pEL3S17-CP1-B0034-UGTCs2-6xHis - 143.pEL3S17-CP8-B0034-UGTCs2-6xHis - 163.pEL3S17-ZCD-His-B0034-UGTCs2-His - Screening gel fail 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His - 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 - Screening gel fail 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - Screening gel fail 219.pSB1C3-Miraculin - 221. pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 201. E.Coli. Nissle - CrtY-CrtI - Screening gel fail</div>'

+

}

+

else if(id == 'd2013810')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-10</h1> Name of participants: Anders Edlund, Marcus H, Hampus.E, Kristoffer L, Ken B-A, Nils, Alexander, Sabri, Pontus <h2>Ongoing constructs:</h2> E. coli: 134. B0034-CBP 135. B0034-CSP 254. Gus 257. p-Toxin-Antitoxin 258. Toxin-Antitoxin 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 259. pEL3S17-B0034-4CL-B0034-STS 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 259. pEL3S17-B0034-4CL-B0034-STS 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His E. coli PK401 dam-: 60. no plasmid <h2>Todays work:</h2> Gel-electrophoresis 134. B0034-CBP* 135. B0034-CSP* 254. Gus 257. p-Toxin-Antitoxin 258. Toxin-Antitoxin *PCR 99% failed 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 115. pSB3K5-J23101-B0034-His-TAL 114. pSB1C3-B0034-TAL(MUT) 120.pSB1C3-B0034-HIS-4CL Amplification PCR 134. B0034-CBP 135. B0034-CSP ON: 60. E. coli dam- Assembly and subcloning: 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 259. pEL3S17-B0034-4CL-B0034-STS 127 + 103 + 36 -> 253. pEL3S17-J23101-B0034-UGTCs2-His 152 + 61 + 111 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 152 + 62 + 111 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 95 + 58 + 36 -> 110.pEL3S17-K206001-B0034-IspA CrtZ + 1 -> 184.pSB1C3-B0034-CrtZ Miraculin + 1 -> 219.pSB1C3-Miraculin O/N Screening: 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His 201. E.Coli. Nissle - CrtY-CrtI DPN1 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 <h2>Results:</h2> Gel electrophoresis: 115 good, to sequencing 114 good, to sequencing 120 pick new clones and screen 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 - Succes 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - fail 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 -fail Characterization experiments Detection of TAL activity through spectroscopy (day 5)</div>'

+

}

+

else if(id == 'd2013811')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-11</h1> Name of participants: Anders E, Mikael S, Nafisa B, Nils, Alexander, Sabri, Malin <h2>Ongoing constructs:</h2> E. coli: 60. PK401 dam- no plasmid 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 91. BBa_J61002-J23106 92. BBa_J61002-J23110 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 244. pSB1C3-Promoter-RBS-Bleomysin 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow 253. pEL3S17-J23101-B0034-UGTCs2-His E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work:</h2> Competent cells: 60. PK401 dam- noplasmid Transformation: 275. PK401 dam- pSBLb4C15-red (dam-) 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) Gel electrophoresis: 111.7 pSBLb4C15 cut with E and P and more. Digest: 111.7 pSBLb4C15 cut with E and P 111.7 pSBLb4C15 cut with SacI and SalI 254. Gus P+X 257. p-Toxin-Antitoxin P+X 258. Toxin-Antitoxin P+X 134. B0034-CBP E+S 1. pSB1C3-red E+S 3. pSB3T5-red E+P PCR purification: 254. pSB1C3-Gus 257. pSB1C3-p-Toxin-Antitoxin (with promotor) 258. pSB1C3-Toxin-Antitoxin 134. pSB1C3-B0034-CBP PCR: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) Plasmidprepp: 108.2.pEL3S17-J23106-B0034-IspA 108.3.pEL3S17-J23106-B0034-IspA 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue Two part assembly: 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow O/N: 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis Sekvensprepp: 220.pSB3K3-J23110-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Re-circularization: 202.pSB4C5.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 Re-streak 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink <h2>Results:</h2> Gel electrophoresis: 111.7 pSBLb4C15 cut with E and P: correct bands! and more.</div>'

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}

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else if(id == 'd2013812')

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{

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ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1> Name of participants: Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B <h2>Ongoing constructs:</h2> E. coli: 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 149. pSB1C3-B0034-Thioredoxin 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 114. pSB1C3-B0034-TAL-mut 115. pSB3K3-J23101-B0034-His-TAL 204. pEL3S17-J23110-B0034-HIS-STS 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work</h2> Ligation: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 134. pSB1C3-B0034-CBP 254. pSB1C3-Gus 257. pSB1C3-p-Toxin-Antitoxin (with promotor) 258. pSB1C3-Toxin-Antitoxin 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Digest: 4.pSB1A3-red 6.pSB1K3 125. pSB1C3-ZCD-His 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Re-streak: 110.pEL3S17-K206001-B0034-IspA 219.pSB1C3-Miraculin 253. pEL3S17-J23101-B0034-UGTCs2-His 275 PK401 dam- pSBLb4C15-red (dam-) 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) O/N Screening: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp Transformation 134. pSB1C3-CBP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Spreading: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem Plasmidprepp: 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Sequence prepp: 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Transformation: 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) PCR: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp <h2>Results:</h2> 222.1.pSB3K3-CP1-B0034-UGTCs2-His failed 222.3.pSB3K3-CP1-B0034-UGTCs2-His failed 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) <h2>Characterization</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Mixing new buffers for western blot Casting LB-agar plates: Chloramphenicol: 55</div>'

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}

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else if(id == 'd2013812')

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{

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ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1> Name of participants: Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B <h2>Ongoing constructs:</h2> E. coli: 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 149. pSB1C3-B0034-Thioredoxin 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 114. pSB1C3-B0034-TAL-mut 115. pSB3K3-J23101-B0034-His-TAL 204. pEL3S17-J23110-B0034-HIS-STS 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work</h2> Ligation: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 134. pSB1C3-B0034-CBP 254. pSB1C3-Gus 257. pSB1C3-p-Toxin-Antitoxin (with promotor) 258. pSB1C3-Toxin-Antitoxin 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Digest: 4.pSB1A3-red 6.pSB1K3 125. pSB1C3-ZCD-His 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Re-streak: 110.pEL3S17-K206001-B0034-IspA 219.pSB1C3-Miraculin 253. pEL3S17-J23101-B0034-UGTCs2-His 275 PK401 dam- pSBLb4C15-red (dam-) 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) O/N Screening: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp Transformation 134. pSB1C3-CBP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Spreading: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem Plasmidprepp: 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Sequence prepp: 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Transformation: 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) PCR: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp <h2>Results:</h2> 222.1.pSB3K3-CP1-B0034-UGTCs2-His failed 222.3.pSB3K3-CP1-B0034-UGTCs2-His failed 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) <h2>Characterization</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Mixing new buffers for western blot Casting LB-agar plates: Chloramphenicol: 55</div>'

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else if(id == 'd2013813')

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ds = '<div id="dairy-text"><h1>Tuesday 2013-08-13</h1> Name of participants: Anders Edlund, Mikael Strandgren, Anton Berglund, Viktor Törnblom, Viktor Blomkvist, Christoffer Ahlström, Stephanie Herman, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L. Lovisa P, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 114.(2-3) pSB1C3-B0034-TAL-mut 115.(1-3) pSB3K3-J23101-B0034-His-TAL 204.(1-3) pEL3S17-J23110-B0034-HIS-STS <h2>Todays work:</h2> O/N Screening glycerolstock: 110.pEL3S17-K206001-B0034-IspA 219.pSB1C3-Miraculin 253.pEL3S17-J23101-B0034-UGTCs2-His Assembly pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink Ligation: 134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product]) 134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product]) 255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product]) 255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product]) 256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product]) 256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product]) Plasmidprepp: 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His Sequencing prep: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 114.(2-3) pSB1C3-B0034-TAL-mut 115.(1-3) pSB3K3-J23101-B0034-His-TAL 204.(1-3) pEL3S17-J23110-B0034-HIS-STS 108.2.pEL3S17-J23106-B0034-IspA 108.3.pEL3S17-J23106-B0034-IspA 163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His Re-streaking: 134. pSB1C3-CBP (CBP1 [134.1] from digested plasmids [PCR-product]) 134. pSB1C3-CBP (CBP2 [134.2] from digested plasmids [PCR-product]) 254. pSB1C3-gus (gus1 [254.1] from digested plasmids [PCR-product]) 254. pSB1C3-gus (gus2 [254.2] from digested plasmids [PCR-product]) 254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product]) 255. pSBLb4C15-pemp (pemp1 [257.1] from digested plasmids [PCR-product]) 255. pSBLb4C15-pemp (pemp2 [257.2] from digested plasmids [PCR-product]) 256. pSBLb4C15-pem (pem1 [258.1] from digested plasmids [PCR-product]) 256. pSBLb4C15-pem (pem2 [258.2] from digested plasmids [PCR-product]) 256. pSBLb4C15-pem (pem3 [258.3] from digested plasmids [PCR-product]) 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Re streak 14/8 or 15/8 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) Transformation & plating 134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product]) 134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product]) 255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product]) 255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product])256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product]) 256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product]) 225. pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 261. pSB3K3-J23110-B0034-HIS-4CL 120. pSB3K3-B0034-HIS-4CL 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 177pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 208. pSB1A3-B0034-Thioredoxin-ZCD-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His Re-streaking 254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product]): 1 clone 255. pSBLb4C15-pemp (pemp3 [257.3] from digested plasmids [PCR-product]): 4 clones O/N: 275. pSBLb4C15-red (dam-) 276. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam) 277. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam) Gradient PCR: CBP (colony PCR from Lb.Reuteri) CSP (colony PCR from Lb.Reuteri) Mcherry (from plasmid preparation) 256. pSBLb4C15-pem <h2>Results</h2> One of the two gels from SDS page was succesfull, still have problems with the buffers <h2>Characterization</h2> Characterization of Resveratrol production through spectroscopy and HPLC Western blot SDS-page on constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL Electro transfer over night <h2>Other experiments</h2> Casting LB-agar plates: Chloramphenicol: 85 </div>'

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}

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else if(id == 'd2013814')

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{

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ds = '<div id="dairy-text"><h1>Wednesday 2013-08-14</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Mikael S, Viktor B, Viktor T, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Peter C, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work:</h2> Re-streak: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 pSB1K3-J23110-B0034-aeBlue (should be subcloned to pSB1C3 after) pSB3K3-J23110-B0034-AmilCP (should be subcloned to pSB1C3 after) pSB3K3-J23110-B0034-asCP (should be subcloned to pSB1C3 after) Re streak 15/8 or 16/8 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink O/N: 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP Streak from frozen stock: 111. pSBLb4C15-red Gradient touchdown colony PCR: CSP Gel Electrophoresis: CBP (colony PCR from Lb.Reuteri) CSP (colony PCR from Lb.Reuteri) Mcherry (from plasmid preparation) 256. pSBLb4C15-pem PCR purification: M1 M2 M3 M4 CB1 CB2 256. pSBLb4C15-pem (P1) Digestion: M3 (x+p) CB1 (x+p) P1 (x+p) 111.7 pSB4C15-red (e+p) Bleomycin resistance cassette (SacI) pEL PCR product with e. coli ori (ClaI) Lb3 reuteri DSM 20016 pVS2 (ClaI) CP promoters with amilGFP, aeBlue,tsPurple Ligation: M3 with pSB1C3, pSBLB4C15 + CP8, CP1 CB1 with pSB1C3 P1 with pSB1C3 pVS2 origin of replication with pEL PCR-product, cut by ClaI CP promoters with amilGFP, aeBlue,tsPurple Frozen stock and plasmid preparation: 275.1 pSBLb4C15-red (dam-)* 275.2 pSBLb4C15-red (dam-) 276. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) *O/N of clone 1 was made from a very small colony, its colour wasn’t as strong as clone 2’s after growing overnight. Transformation: 109.pEL3S17-K206000-B0034-IspA x 110.pEL3S17-K206001-B0034-IspA x 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His x 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His x 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His x 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His x 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His x 184.pSB1C3-B0034-CrtZ x 208.pSB1A3-B0034-Thioredoxin-ZCD-His x 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His x 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His x 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His x 267.psB3K3-K206000-B0034-IspA x 268.psB3K3-K206001-B0034-IspA x 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His x 272 pSB1K3-B0034-Thioredoxin-ZCD-His x 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His x Plasmid prepp: 280. pSB1A3-B0034-aeBlue (should be subcloned to pSB1C3 after, give Erik the subclones version) <h2>Results:</h2> Gel Electrophoresis: CBP (colony PCR from Lb.Reuteri) CB1: 54.3o C (NC ok!), CB2: 63.3o C (NC ok!) → Correct bands!! CSP (colony PCR from Lb.Reuteri) CS1: 55.7o C (NC ok!), CS2: 65.6o C (NC ok!) → No bands!! (not even primer dimer) Mcherry (from plasmid preparation) M1: 45.0o C (NC ok!), M2: 47.9o C (NC ok!), M3: 50.5o C (NC ok!), M4: 54.3o C (NC ok!) → Correct bands!! 256. pSBLb4C15-pem: P1: 47.9o C (NC ok!) → Correct bands!! PCR purification: M1: 65.9 ng/µl → Store in freezer! M2: 200.6 ng/µl → Store in freezer! M3: 136.8 ng/µl → For digest! → Store in freezer! M4: 49.5 ng/µl → Store in freezer! CB1: 90.8 ng/µl → For digest! → Store in freezer! CB2: 56.7 ng/µl → Store in freezer! P1: 56.2 ng/µl → For digest! → Store in freezer! Plasmid preparation: 275.1 PK401 dam- pSBLb4C15-red (dam-): 13.5 ng/µl 275.2 PK401 dam- pSBLb4C15-red (dam-): 27.9 ng/µl 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-): 18.3 ng/µl 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-): 26.6 ng/µl Western blot: Transfer overnight not complete but continued with anyway Very promising results, bands can be seen around 50 kDa but the ladder is pretty weak so we can’t be absolutely sure. Looks like the J23101 promotor does’nt work. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC Blotting and development of constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL <h2>Other experiments:</h2> Making MRS-broth (2x600 ml) New buffers for western blot made </div>'

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else if(id == 'd2013815')

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{

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ds = '<div id="dairy-text"><h1>Thursday 2013-08-15</h1> Name of participants: Christoffer Ahlström, Stephanie Herman, Alona Nyberg, Mikael Strandgren, Alexander, Malin, Pontus, Niclas, Christoffer, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H., Peter C, Lovisa P, Theodor L <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Digestions: Erythromycin resistance cassette from pLUL631 (SacI, Tango buffer) Bleomycin resistance cassette (SalI, FD buffer) O/N Screening glycerolstock: 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Re-streak: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 184.pSB1C3-B0034-CrtZ 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 110.pEL3S17-K206001-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Plasmid prep: 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP Transformation: 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 292. PK401 dam- pSBLb4C15-CP1-mCherry (dam-) 294. PK401 dam- pSBLb4C15-CP8-mCherry (dam-) Ligations: pSBLB4C15 and Bleomycin resistance cassette cut with SacI and SalI Spreading of strains: 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry E. coli PK401 dam-: 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry Screening PCR and gel electrophoresis <h2Results:</h2> Yesterdays buffers for western blot resulted in smear. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Making MRS-agar plates: 47 Chloramphenicol 47 Without antibiotics Testing yesterdays buffers for western blot. New separation- and stacking buffers for western blot with a factor 1000 concentration of Tris.</div>'

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}

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else if(id == 'd2013816')

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{

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ds = '<div id="dairy-text"><h1>Friday 2013-08-16</h1> Name of participants: Anton Berglund, Stephanie Herman, Mikael Strandgren, Alexander, Pontus, Nils, Christoffer, Sabri, Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L, Peter C <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> O/N Screening & glycerolstock: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 184.pSB1C3-B0034-CrtZ 208. pSB1A3-B0034-Thioredoxin-ZCD-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 2 74.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red Digestion Erythromycin cassette amplicon from pLUL631 (SalI) Ligation pSB1C3-pemp 298. pSBLb4E15-red (E-cassette amplicon from pLUL631 with pSBLb4C15 (SacI + SalI)) 297. pSBLb4B15-red (Bleomycin cassette with pSBLb4C15 (SacI + SalI)) Plasmid preparation: 204.1 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 204.4 pEL3S17-J23110-B0034-HIS-STS 254.1 pSB1C3-gus 254.2 pSB1C3-gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 255.3 pSBLB4C15-pemp 255.4 pSBLB4C15-pemp O/N: 204.1 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 204.4 pEL3S17-J23110-B0034-HIS-STS 254.1 pSB1C3-gus 254.2 pSB1C3-gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 255.3 pSBLB4C15-pemp 255.4 pSBLB4C15-pemp 257.1 pSB1C3-pemp 257.2 pSB1C3-pemp 257.3 pSB1C3-pemp 280. pSB1K3-J23110-B0034-aeBlue 281. pSB3K3-J23110-B0034-amilCP 282. pSB3K3-J23110-B0034-asCP Transformation: 297. PK401 dam- pSBLb4B15-red 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 155pSB1C3-j23110-RBS-meffRed 156pSB1C3-j23106-RBS-meffRed 157pSB1C3-j23110-RBS-spisPink 158pSB1C3-j23106-RBS-spisPink 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 247pSB1C3-J23110-RBS-tsPurple 248pSB1C3-J23106-RBS-tsPurple 249pSB1C3-J23110-RBS-scOrange 250pSB1C3-J23106-RBS-scOrange 251pSB1C3-J23110-RBS-fwYellow 252pSB1C3-J23106-RBS-fwYellow 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS <h2>Results:</h2> Plasmid prep: 204.1 pEL3S17-J23110-B0034-HIS-STS: 9.7 ng/µl 204.3 pEL3S17-J23110-B0034-HIS-STS: 11.9 ng/µl 204.4 pEL3S17-J23110-B0034-HIS-STS: 7.6 ng/µl 254.1 pSB1C3-gus: 6.7 ng/µl 254.2 pSB1C3-gus: 11.5 ng/µl 255.1 pSBLB4C15-pemp: 9.9 ng/µl 255.2 pSBLB4C15-pemp: 6.3 ng/µl 255.3 pSBLB4C15-pemp: 5.0 ng/µl 255.4 pSBLB4C15-pemp: 4.6 ng/µl Since the concentrations were so low a new set of overnight cultures were prepared. Spectophotometry: Inconclusive results. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC Testing of new buffers for western blot. <h2>Other experiments</h2> Making MRS-broth (2x600 ml) Making LB-agar plates: 173 Chloramphenicol (chloramphenicol was forgotten in 26 of the plates, but they had already been mixed with 25 others, so also these had to be discarded. 5 plates of doubtful antibiotic content had already been used by the carotenoid group, they were probably ones with ab. E. colis containing pSB3K3 were spread on some of the mixed plates.) 28 Kanamycin</div>'

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}

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else if(id == 'd2013817')

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{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-17</h1> Name of participants: Anders Edlund, Alexander, Pontus, Sabri, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Restreak 254.3 pSB1C3-Gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 297. pSBLb4B15-red 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS Plasmid preparation: 254.1 pSB1C3-Gus 254.2 pSB1C3-Gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 257.1 pSB1C3-pemp 257.2 pSB1C3-pemp 184.pSB1C3-B0034-CrtZ 280. pSB1K3-J23110-B0034-aeBlue 281. pSB3K3-J23110-B0034-amilCP 282. pSB3K3-J23110-B0034-asCP Digest: 147.pSB1C3-B0034-Thioredoxin (X,P) Ligation: 147.pSB1C3-B0034-Thioredoxin, 91.BBa_J61002 (amp)-J23106, 36.pEL3S17-red ---> 300.pEL3S17-J23106-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin, 92.BBa_J61002 (amp)-J23110, 36.pEL3S17- red ---> 301.pEL3S17-J23110-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin, 103.pSB1K3-J23101, 36.pEL3S17-red ---> 299.pEL3S17-J23101-B0034-Thioredoxin Transformation: 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin <h2>Results:</h2> Plasmid preparation: 254.1 pSB1C3-Gus 110 ng/uL 254.2 pSB1C3-Gus 132 ng/uL 255.1 pSBLB4C15-pemp156 ng/uL 255.2 pSBLB4C15-pemp 158 ng/uL 257.1 pSB1C3-pemp 146 ng/uL 257.2 pSB1C3-pemp 140 ng/uL</div>'

+

}

+

else if(id == 'd2013818')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1> Name of participants: Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H. <h2>Ongoing constructs:</h2> <h2>E. coli:</h2> 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> ON: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry pSBLB4C15-red pSBLB4C15-red dam- Restreak: 68.1 pSB3K3-B0034-BFP 68.2 pSB3K3-B0034-BFP 68.4 pSB3K3-B0034-BFP 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.pSB1C3-B0034-Thioredoxin 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.pSB3K3-K206000-B0034-IspA 268.pSB3K3-K206001-B0034-IspA 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.pEL3S17-J23106-B0034-Thioredoxin PCR: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Digest: 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Ligation: 4.pSB1A3-red 7.pSB3K3-red 36.pEL3S17-red 91.j23106 92.j23110 103.pSB1K3-j23101 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Plasmidprep: 184.pSB1C3-B0034-CrtZ Transformation: 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 302. idi (mut)-B0034-Ispa 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 305.pSB1A3-j23110-B0034-CrtZ O/N screening: 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <h2>Results:</h2> Failed: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'

+

}

+

else if(id == 'd2013819')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1> Name of participants: Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Screening 134.1 pSB1C3-CBP 254 pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI 254.2 pSB1C3-gus: XbaI & PstI 255.1 pSBLB4C15-pemp: EcoRI & PstI 255.2 pSBLB4C15-pemp: EcoRI & PstI 257.1 pSB1C3-pemp: EcoRI & PstI 257.2 pSB1C3-pemp: EcoRI & PstI Frozen stock: 134.1 pSB1C3-CBP 275. pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 1 ml overnight culture: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP Plasmid preparation 111.7(1) pSBLB4C15-red 111.7(2) pSBLB4C15-red 134.1 pSB1C3-CBP 275 pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Overnight culture: Lb11.2 plantarum 36E-no plasmid Phusion PCR: usp45 (synthesized signal peptide from Lactococcus lactis) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ digest 36. pEL3S17-red Ligation 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results:</h2> Screening 134.1 pSB1C3-CBP: showed the correct band! 254 pSB1C3-gus: showed a very weak band, not sure if correct! 288.1 pSB1C3-pem: showed the correct band! 288.2 pSB1C3-pem: showed the correct band! 288.3 pSB1C3-pem: did not show the correct band! 288.4 pSB1C3-pem: showed the correct band! 289.1 pSB1C3-mCherry: showed the correct band! 289.2 pSB1C3-mCherry: did not show the correct band! 289.3 pSB1C3-mCherry: showed the correct band! 289.4 pSB1C3-mCherry: showed the correct band. 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! 257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! Plasmid preparation 111.7(1) pSBLB4C15-red: 81.7 ng/µl 111.7(2) pSBLB4C15-red: 93.9 ng/µl 134.1 pSB1C3-CBP: 163.8 ng/µl 275 pSBLB4C15-red (dam-): 92.4 ng/µl 288.1 pSB1C3-pem: 211.2 ng/µl 288.2 pSB1C3-pem: 193.2 ng/µl 288.4 pSB1C3-pem: 193.9 ng/µl 289.1 pSB1C3-mCherry: 230.4 ng/µl 289.3 pSB1C3-mCherry: 229.7 ng/µl 289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'

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}

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else if(id == 'd2013820')

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{

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ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1> Name of participants: Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Characterisation of the CP promoters with facs: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP Digestion: USP. with E, P 191. pSB1A2-fusionGFP with X, P 280. pSB1A3-B0034-aeBlue with X, P 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin Making competent Lactobacillus reuteri Lb12.1 reuteri DSM 20016 plasmid free: eight Lb12.2 reuteri DSM 20016 plasmid free: eight Electroporation Lb3.2 reuteri DSM 20016 pVS2: two 111.7(1) pSBLB4C15-red: one 111.7(2) pSBLB4C15-red: two 255.1 pSBLB4C15-pemp: one 255.2 pSBLB4C15-pemp: one 275 pSBLB4C15-red (dam-): three <h2>Results:</h2> Electroporation Lb3.2 reuteri DSM 20016 pVS2: one of the two survived 111.7(1) pSBLB4C15-red: survived 111.7(2) pSBLB4C15-red: one of the two survived 255.1 pSBLB4C15-pemp: survived 255.2 pSBLB4C15-pemp: survived 275 pSBLB4C15-red (dam-): two of the three survived</div>'

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}

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else if(id == 'd2013821')

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{

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ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1> Name of participants: Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 204(1-4). pEL3S17-J23110-B0034-His-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Digestion: usp45: XbaI & AgeI 191.1 pSB1A2-fusionGFP: XbaI, PstI 191.1 pSB1A2-fusionGFP: NgoMIV & PstI 275. pSBLB4C15-red (dam-): EcoRI & PstI 289.1 pSB1C3-mCherry: NgoMIV & PstI 289.1 pSB1C3-mCherry: XbaI, PstI 297.1 pSBLB4B15-red (dam-): EcoRI & PstI Ligation: pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1) pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1) pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1) pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1) pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1) pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1) pSB1C3-usp45 (1+usp45) pSB1C3-usp45 (6+usp45) pSBLb4C15-CP1-B0034-BFP pSBLb4C15-CP8-B0034-BFP pSBLb4C15-CP11-B0034-BFP pSBLb4C15-CP29-B0034-BFP pSBLb4C15-CP30-B0034-BFP pSBLb4C15-CP41-B0034-BFP pSBLb4C15-CP44-B0034-BFP pSBLb4C15-CP11-aeblue pSBLb4C15-CP30-aeblue pSBLb4B15-CP11-mcherry pSBLb4B15-CP30-mcherry pSBLb4B15-CP11-tspurple pSBLb4B15-CP30-tspurple pSBLb4B15-CP11-amilCP pSBLb4B15-CP30-amilCP pSBLb4B15-CP11-amilGFP pSBLb4B15-CP30-amilGFP 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Transformation: 108.pEL3S17-J23106-B0034-IspA 141.pRL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 204(1-4) Re-streak: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Screen/ ON: 219.pSB1C3-Miraculin 304.pSB1A3-J23106-B0034-CrtZ 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 280. pSB1A3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP Plasmid prep. 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His Mutagenesis PCR: 336. pSB1C3-spisPink 337. pSB1C3-scOrange 338. pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 257. pSB1C3-pemp 288. pSB1C3-pem <h2>Other experiments:</h2> Phosphorylation of primers (20µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R Cleaning of electroporation cuvettes</div>'

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}

+

else if(id == 'd2013822')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1> Name of participants: Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Competent cell preparation: Lb11.1 plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Lb12.2 reuteri DSM 20016 no plasmid Digest: 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP <strike>3.2. pVS2 (died in electroporation) 310. pSBLB4B15-CP11-fusionGFP (died in electroporation) 318. pSBLB4B15-CP11-mCherry (died in electroporation) 320. pSBLB4B15-CP11-amilGFP (died in electroporation) 313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation) 317. pSBLB4B15-CP30-tsPurple (died in electroporation) 331. pSBLB4C15-CP41-BFP (died in electroporation) 332. pSBLB4C15-CP44-BFP (died in electroporation)</strike> + other E. coli transformations Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin <h2>Results:</h2> Mutagenesis PCR: 257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do. 288. pSB1C3-pem: --”-- Transformation: 11.1 Lb. plantarum 36E: 3.2 pVS2: survived electroporation 3.2 pVS2: died during electroporation 310. pSBLB4B15-CP11-fusionGFP: died during electroporation 311. pSBLB4B15-CP30-fusionGFP: survived electroporation 312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation 313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation 316. pSBLB4B15-CP11-tsPurple: survived electroporation 317. pSBLB4B15-CP30-tsPurple: died during electroporation 12.2 Lb. reuteri DSM 20016: 3.2 pVS2: survived electroporation 318. pSBLB4B15-CP11-mCherry: died during electroporation 319. pSBLB4B15-CP30-mCherry: survived electroporation 320. pSBLB4B15-CP11-amilGFP: died during electroporation 321. pSBLB4B15-CP30-amilGFP: survived electroporation 322. pSBLB4B15-CP11-amilCP: survived electroporation 323. pSBLB4B15-CP30-amilCP: survived electroporation 324. pSBLB4C15-CP11-aeBlue: survived electroporation 12.1 Lb. reuteri DSM 20016: 325. pSBLB4C15-CP30-aeBlue: survived electroporation 326. pSBLB4C15-CP1-BFP: survived electroporation 327. pSBLB4C15-CP8-BFP: survived electroporation 328. pSBLB4C15-CP11-BFP: survived electroporation 329. pSBLB4C15-CP29-BFP: survived electroporation 330. pSBLB4C15-CP30-BFP: survived electroporation 331. pSBLB4C15-CP41-BFP: died during electroporation 332. pSBLB4C15-CP44-BFP: died during electroporation <h2>Characterization:</h2> Western blot analysis (SDS-page) on constructs: 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) (neg.contr) <h2>Other experiments:</h2> Phosphorylation of primers (60 µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R</div>'

+

}

+

else if(id == 'd2013823')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1> Name of participants: Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> E. coli (D5α): 111.7 pSBLb4C15-red 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Gel electrophoresis: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites) Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Touchdown with Tm1+2 and Tm1+4. Transformation: 102.pSB1C3-CrtE 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208. pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 300. pEL3S17-J23106-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin Re-streak: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Screening O/N: 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 222.pSB3K3-CP1-B0034-UGTCs2-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin O/N: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Glycerol stock Plasmidprep/sekvensprep: 108.pEL3S17-J23106-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 299.pEL3S17-J23101-B0034-Thioredoxin <h2>Results:</h2> Gel electrophoresis: 257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 111.7 pSBLb4C15-red (cut with E and P): No band *→ redo mutagenesis PCR with higher temperatures</div>'

+

}

+

else if(id == 'd2013824')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-24</h1> Name of participants: Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> Escherichia coli 257. pSB1C3-pemp 288. pSB1C3-pem 136. pSB1C3-B0034-USP pSB1C3-B0034-usp45 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS Lactobacillus reuteri: Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP Lactobacillus plantarum: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Digest: 200.pSB1C3-B0034-asCP E,X 148.pSB1C3-RBS-gfasPurple E,X 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) Ligation: 200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→ → 343.J23106-B0034-asCP 148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→ → 160.j23106-RBS-gfasPurple 148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→ → 159j23110-RBS-gfasPurple 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→ →215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His Transformation: 223.pSB3K3-CP8-B0034-UGTCs2-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 343.pSB1C3-J23106-B0034-asCP 160.pSB1C3-j23106-RBS-gfasPurple 159.pSB1C3-j23110-RBS-gfasPurple 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 336.pSB1C3.spisPink 337.pSB1C3.scOrange 284.pSB1C3.B0034-aeBlue 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 284. pSB1C3-B0034-aeBlue 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP O/N screening: 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 136. 312 313 315 321 325 327 332 317 O/N: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Plasmid prepp & sequence prepp 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Gel electrophoresis: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R + E. coli transformations PCR-purification: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R DpnI: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R <h2>Results:</h2> Transformations: All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. Gel electrophoresis: All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite. The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'

+

}

+

else if(id == 'd2013825')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1> Name of participants: Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Digest: 147.pSB1C3-B0034-Thioredoxin Ligate: 147.pSB1C3-B0034-Thioredoxin+CP1→ → 346.pSB1C3-CP1-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP8→ →347.pSB1C3-CP8-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP41→ →348.pSB1C3-CP41-B0034-Thioredoxin Transformation: 1.pSB1C3-Red (competens test) 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ 336.pSB1C3-spisPink 337pSB1C3-scOrange 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348.pSB1C3-CP41-B0034-Thioredoxin 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple Re-streak: 284. pSB1C3-B0034-aeBlue Sequenceprep: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) </div>'

+

}

+

else if(id == 'd2013826')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1> Name of participants: Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin <h2>Todays work</h2> Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 139. pSB3K3-J23101-B0034-BFP PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Slovenian 4CL with His-primers Gel electrophoresis: Erythromycin-cassette PCR-product from Lb2.1 pLUL631 Erythromycin-cassette PCR-product from Lb2.2 pLUL631 Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R Assembly & transformation: 349 Mutagenesis 338.pSB1C3-meffBlue Re streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP Plasmidprepp: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin O/N screen 284. pSB1C3-B0034-aeBlue Screening: 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348. pSB1C3-CP41-B0034-Thioredoxin O/N: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades) Re-streak 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades) Sekvensprep: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation: 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → →215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His <h2>Results:</h2> Gel electrophoresis: Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly Erythromycin-cassette PCR-product: --”-- Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed 77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp. 269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made. <h2>Characterization:</h2> Western blot sample preparation: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparing MRS-agar Preparing low salt LB-agar</div>'

+

}

+

else if(id == 'd2013827')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-27</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> O/N: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 Transformation: Chromoproteins into pSB3K3 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1K3-CP1-B0034-Thioredoxin 347.pSB1K3-CP8-B0034-Thioredoxin 348.pSB1K3-CP41-B0034-Thioredoxin Gel electrophoresis: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Screening PCR: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ Sekvensprep: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 284. pSB1C3-B0034-aeBlue Re-streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures Tm1 and +5°C (with touchdown)) Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml 100 µg/ml <h2>Results</h2> Gel electrophoresis: 77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp 77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp <h2>Other experiments</h2> Preparing test plates, to determine minimum antibiotic concentration: Lb-erythromycin: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml MRS-zeocin: 50 µg/ml 100 µg/ml</div>'

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}

+

else if(id == 'd2013828')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-28</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin <h2>Todays work</h2> Gel electrophoresis: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 77. pSB4C15-red (annealing at Tm1): 77. pSB4C15-red (annealing at Tm1+5): Ligation: 352. pSBLbC3-red (pEL4C15 (ClaI) + pVS2 (ClaI)) 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R (Tm1) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digest 36. pEL3S17-red Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results</h2> Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml: Growth 10 µg/ml: Growth 20 µg/ml: Growth 50 µg/ml: Still some growth, but less → do new plates with higher conc. and try 50 µg/ml-plates from Erik Gullberg in case our antibiotic has lost efficiency. - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml: Growth 100 µg/ml: Growth (Plantarum grew less, but did so also on antibiotic free plates.) - lactobacillus (Lb4., Lb11. and Lb12.) onto antibiotic-free MRS-plates: Everything grew on Ab-, plantarum slightly less. Gel electrophoresis: 351.1 pSB1C3-pemp-mut1: A weak band at ~1000 bp 351.2 pSB1C3-pemp-mut1: Nothing 77. pSB4C15-red (annealing at Tm1): Nothing → do a new PCR at TM1. 77. pSB4C15-red (annealing at Tm1+5): Nothing. 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: Failed 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: Failed 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: Failed Re-streak: 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <h2>Other experiments</h2> Preparation of new test-plates: LB-erythromycin: 100 µg/ml 200 µg/ml</div>'

+

}

+

else if(id == 'd2013829')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-29</h1> Name of participants: Mikael Strandgren, Viktor Blomkvist, Alona Nyberg, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> E. coli: 353. pSBLb4E15 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His <h2>Todays work</h2> Transformation: 353. pSBLb4E15 Plasmidprep: 223 346 <h2>Results</h2> Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 100 µg/ml: Weak growth 200 µg/ml: Possibly no growth, let them “grow” some more. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparation of new test-plates: MRS-Zeocin: 250 µg/ml </div>'

+

}

+

else if(id == 'd2013830')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-30</h1> Name of participants: Mikael Strandgren, Viktor Björklund, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 77. pSB4C15-red 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Lb9. faecalis JH2-2 pAMbeta <h2>Todays work</h2> Gel extraction: 77. pSB4C15-red (annealing at Tm1): PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R* *Since the product from monday disappeared. PCR purification: Lb9. faecalis JH2-2 pAMbeta1-ori Digestion: Lb9. faecalis JH2-2 pAMbeta1-ori (PCR product)(XbaI and BglII) 77. pSB4C15-red (PCR product) (NheI and BglII) Ligation: 169. pSBLbEcC2-red (shuttle-vector with pAMbeta and e. coli replicon) <h2>Results</h2> Western blot: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS failed. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd2013831')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-31</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> Transformation: 111. pSBLb4C15-red 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) 386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1) Gel electrophoresis: 169. pSBLbEcC2-red (ligation) Ligation: 111. pSBLb4C15-red (pEL+ replicon from pJP) 352. pSBLbC3-red (pEL+ replicon from pVS2) <h2>Results</h2> Gel electrophoresis: 169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'

+

}

+

else if(id == 'd201391')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-01</h1> Name of participants: Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 275. pSBLb4C15-red (dam-) 352. pSBLbC3-red 406. pSBLbC3-red (dam-) 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> Transformation: 111. pSBLb4C15-red (pEL+ replicon from pJP) 275. pSBLb4C15-red (dam-) 352. pSBLbC3-red (pEL+ replicon from pVS2) 406. pSBLbC3-red (dam-) Screening: 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2) Re-streak: 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) <h2>Results</h2> First screening failed (weird gel) <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201392')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-02</h1> Name of participants: Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work: </h2> O/N: 169. pSBLbEcC2-red Mutagenesis PCR: 111.7 pSBLb4C15-red * *with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night. Ligation: 111. pSBLb4C15-red (pEL4C15+replicon from pJP059)* 352. pSBLbC3-red* *New attempts at ligation, just in case. <h2>Results: </h2> Gel electrophoresis: 111.7 pSBLb4C15-red: No bands Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS failed. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201393')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1> Name of participants: Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 261. pSB3K3-J23110-B0034-His-4CL 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work: </h2> Frozen stock and plasmid preparation: 169. pSBLbEcC2-red Gel electrophoresis: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) Transformation: 111. pSBLb4C15-red (pEL4C15+replicon from pJP059)* *New attempts at ligation, just in case. Sequencing preparation: 169. pSBLbEcC2-red <h2>Characterization experiments:</h2> Western blot sample preparation on construct: 335. pSB3K3-J23101-HIs-STS <h2>Results: </h2> Frozen stock and plasmid preparation: 169. pSBLbEcC2-red: 44.2 ng/µl 169. pSBLbEcC2-red: 47.8 ng/µl Gel electrophoresis: 111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'

+

}

+

else if(id == 'd201394')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1> Name of participants: Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> Assembly: 353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old) Trasformation: 111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP) 353. pSBLb4E15-red PCR-purification: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) DpnI-treatment: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'

+

}

+

else if(id == 'd201395')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1> Name of participants: Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L <h2>Ongoing constructs:</h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work</h2>First day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201396')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L <h2>Ongoing constructs: </h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> PCR-purification and recircularization: Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30) O/N: 111.7 pSBLb4C15-red* *to do a new plasmid preparation since old may have corrupt red-cassette/contamination) Streak from frozen stock: 111.7 pSBLb4C15-red* * to test if red-cassette has been damaged, or possibly the stock contaminated. Second day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201397')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1> Name of participants: Mikael Strandgren, Christoffer L <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work:</h2> Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE) 355.2 pSB3K3-CP29-mCherry 355.3 pSB3K3-CP29-mCherry 357.3 pSB3K3-CP30-usp45-mCherry 357.4 pSB3K3-CP30-usp45-mCherry 358.1 pSB3K3-CP29-usp45-mCherry 358.2 pSB3K3-CP29-usp45-mCherry 363. pSB3K3-CP30-tspurple 364. pSB3K3-CP29-tspurple 365. pSB3K3-CP11-tspurple 366. pSB3K3-CP30-amilCP 367. pSB3K3-CP29-amilCP 368. pSB3K3- CP11-amilCP 369. pSB3K3-CP30-aeblue 370. pSB3K3-CP29-aeblue 371. pSB3K3-CP11-aeblue Assembly: 407. pSB1C3-CP29-Mcherry* 408. pSB1C3-CP30-usp45-mCherry* 409. pSB1C3-CP29-usp45-mCherry* 410. pSB1C3-CP30-tsPurple* 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP* 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue* 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old) * pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today. Transformation 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette. O/N: 111.7 pSBLb4C15-red Third day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Results: </h2> O/N: 111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today. Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl 355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl 355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl 357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl 357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl 358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl 358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl 363. pSB3K3-CP30-tspurple: 75.5 ng/µl 364. pSB3K3-CP29-tspurple: 43.9 ng/µl 365. pSB3K3-CP11-tspurple: 47.3 ng/µl 366. pSB3K3-CP30-amilCP: 74.5 ng/µl 367. pSB3K3-CP29-amilCP: 77.2 ng/µl 368. pSB3K3- CP11-amilCP: 34.3 ng/µl 369. pSB3K3-CP30-aeblue: 37.4 ng/µl 370. pSB3K3-CP29-aeblue: 60.3 ng/µl 371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'

+

}

+

else if(id == 'd201398')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-08</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work: </h2> Transformations: 411. pSB1C3-CP29-tsPurple * 412. pSB1C3-CP11-tsPurple * 414. pSB1C3-CP29-amilCP * 415. pSB1C3-CP11-amilCP * 417. pSB1C3-CP29-aeBlue * 418. pSB1C3-CP11-aeBlue * * The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good. ON: 353. pSBLB4E15-red <h2>Results: </h2> Transformation 411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today. 412. pSB1C3-CP11-tsPurple: “ 414. pSB1C3-CP29-amilCP “ 415. pSB1C3-CP11-amilCP “ 417. pSB1C3-CP29-aeBlue “ 418. pSB1C3-CP11-aeBlue “ 419. pSBLbEcE-red: Growth covering the plates, only white colonies. pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'

+

}

+

else if(id == 'd201399')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1> Name of participants: Sabri, Christoffer L <h2>Todays Work:</h2> Digest 338.pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation 338.pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Fourth day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. </div>'

+

}

+

else if(id == 'd2013910')

+

{

+

ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1> Name of participants: Sabri, Christoffer L <h2>Todays Work:</h2> Re-streak 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue Fifth day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. </div>'

+

}

+

else if(id == 'd2013911')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1> Name of participants: Sabri <h2>Todays Work:</h2> O/N: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd2013912')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1> Name of participants: Sabri <h2>Todays Work:</h2> Plasmid prep: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue Sequence prep: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd20131013')

+

{

+

ds ='<h1>European Jamboree Lyon end</h1>';

+

}

+

else if(id == 'd20131012')

+

{

+

ds ='<h1>European Jamboree Lyon</h1>';

+

}

+

else if(id == 'd20131011')

+

{

+

ds ='<h1>European Jamboree Lyon start</h1>';

+

}

+

else if(id == 'd2013104')

+

{

+

ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';

+

}

+

else if(id == 'd2013102')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1> Name of participants: Anders Edlund <h2>Ongoing constructs</h2> Lb47. reuteri 100-23 pSBLbE-red <h2>Todays work</h2> Sequencing preparation: Lb47. reuteri 100-23 pSBLbE-red </div>'

+

}

+

else if(id == 'd2013101')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1> Name of participants: Anders Edlund <h2>Ongoing constructs </h2> Lb47. reuteri 100-23 pSBLbE-red <h2>Todays work</h2> Screening PCR: Lb47. reuteri 100-23 pSBLbE-red <h2>Results</h2> Transformation: To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2: Growing Lb13. pJP059: Growing 420. pSBLbE-CP11-amilCP: Not growing 435. pSBLbC-CP29-amilCP: Not growing 440. pSBLbC-red: Not growing 441. pSBLbE-red: Growing All negative controls were clear, and the positive controls grew. </div>'

+

}

+

else if(id == 'd2013928')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1> Name of participants: Viktor Blomkvist, Mikael Strandgren, Erik Edlund <h2>Ongoing constructs</h2> E. coli: 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red L. reuteri: Lb3. pVS2 Lc. lactis: Lb13. pJP059 <h2>Todays work</h2> Transformation: To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2 Lb13. pJP059 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red </div>'

+

}

+

else if(id == 'd2013927')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> Lb4. reuteri 100-23 no plasmid Lb11. Plantarum 256 no plasmid Lb12. Reuteri DSM20016 no plasmid 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Todays work</h2> Overnight: Lb4. reuteri 100-23 no plasmid Lb11. Plantarum 256 no plasmid Lb12. Reuteri DSM20016 no plasmid Streak from frozen stock (for photos): 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red </div>'

+

}

+

else if(id == 'd2013924')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1> Name of participants: Erik Edlund, Mikael Strandgren, Viktor Blomkvist <h2>Ongoing constructs</h2> <h2>Results</h2> Transformation: No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday. </div>'

+

}

+

else if(id == 'd2013921')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1> Name of participants: Erik Edlund, Mikael Strandgren, Viktor Blomkvist <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Todays work</h2> Transformation To L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2 (positive control) 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Other experiments</h2> Making MRS-agar plates: Erythromycin Chloramphenicol Antibiotic-free </div>'

+

}

+

else if(id == 'd2013920')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1> Name of participants: Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> Plasmid preparation: 435. pSBLbC-CP29-amilCP </div>'

+

}

else if(id == 'd2013919')

{

ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';

}

+

else if(id == 'd2013918')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1> Name of participants: Viktor Blomkvist <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> O/N: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013917')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> Re-streak: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013916')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP 440. pSBLbC-red <h2>Todays work</h2> Plasmid preparation: 440. pSBLbC-red Assembly and transformation: 435. pSBLbC-CP29-amilCP </div>'

+

}

else

{

@@ Line 1: / Line 1: @@
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 var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
-var days_with_dirys = new Array('t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717'
+var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');
-,'t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385'
+var inputs = 106;
-,'t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820'
-,'t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399'
-,'t2013910','t2013911','t2013912','t2013913','t2013914','t2013915','t2013919');
-var inputs = 93;
 Date.prototype.getWeek = function()
@@ Line 151: / Line 147: @@
    eventString =  '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
    document.getElementById('file_holder').innerHTML = eventString;
+document.getElementById('calendar').style.marginBottom="350px";
   }
@@ Line 188: / Line 185: @@
      eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
     }
+document.getElementById('calendar').style.marginBottom="350px";
    document.getElementById('file_holder').innerHTML = eventString;
    eventString = '';
@@ Line 211: / Line 209: @@
   if(id == 'd2013630')
   {
-  ds = '<h2>Sunday 2013-06-30</h2><p><strong>Name of participants:</strong> Magnus B, Alexander W, Nils A, Mikael Strandgren (MS), Anders Edlund (AE)<br><br>'+
+  ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Mikael S, Anders E<br><br><h2>Ongoing constructs:</h2><b>E. coli:</b><br>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><b>L. reuteri:</b><br>Lb1. CF48-pLR581<br>Lb2. 1063-pLUL631<br>Lb3. DSM 20016-pVs2<br>Lb4. 100-23-noplasmid<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br>Lb8. 100-23-pGT232<br>Lb12. DSM 20016-noplasmid<br>Lb14. DSM 20016-pLUL631<br><br><b>L. plantarum:</b><br>Lb5. 256-rifR-pAMβ1<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br>Lb16. MG1363-noplasmid<br><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br>Lb9. JH2-2 pAMβ1<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>75. pEL3A15 - CrtB<br><br><b>Digest</b><br>27. pEL3K16-red<br>51.pEL3C18-red<br><br><b>Ligation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly.<br><br><b>O/N Screening</b><br>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results</h2><br><b>Re-streak</b><br>Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br></div>';
- '<h3>Ongoing constructs:</h3>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>'+
- '51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
- '21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
- '42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>'+
- '65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>'+
- '70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>'+
- '74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+
- '<b>L. reuteri:</b><br>Lb1. CF48-pLR581<br>Lb2. 1063-pLUL631<br>Lb3. DSM 20016-pVs2<br>'+
- 'Lb4. 100-23-noplasmid<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br>Lb8. 100-23-pGT232<br>'+
- 'Lb12. DSM 20016-noplasmid<br>Lb14. DSM 20016-pLUL631<br><br><strong>L. plantarum:</strong><br>Lb5. 256-rifR-pAMβ1<br><br>'+
- '<b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br>Lb16. MG1363-noplasmid<br><br>'+
- 'Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br>Lb9. JH2-2 pAMβ1<br><br><h3>Todays work</h3><br><h3>Transformation</h3>'+
- '61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>'+
- '67. pSB1C3-Cp44<br>77. pSB4C15-red<br>'+
- '75. pEL3A15 - CrtB<br><br><h3>Digest</h3>27. pEL3K16-red<br>51.pEL3C18-red<br><br>'+
- '<h3>Ligation</h3>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
- '66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly.<br><br><h3>O/N Screening</h3>'+
- '25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
- '21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h3>Results</h3><br><b>Re-streak</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>'+
- '20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br>';
   }
   else if(id == 'd2013629')
   {
- ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><p><b>Name of participants:</b> Magnus B, Alexander W, Nils'+ 'A, Anders E, Mikael S<br><br>'+
+	ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Anders E, Mikael S<br><br><h2>Ongoing constructs:</h2><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli (strain D5α):</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Todays work</h2><b>PCR purification:</b><br>CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br><b>Digestion:</b><br>27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br><b>Ligation:</b><br>24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br><b>Transformation:</b><br>75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br><b>Re-streak:</b><br>1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br><b>Plasmid preparation:</b><br>42. pSB4S15-red<br><br><b>O/N Screening PCR:</b><br>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>(19,20,21(with DMSO dilution series(7,9,12))<br><br><b>PCR (screening?):</b><br>57.B0034-Idi<br>IspA<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br><h2>Results</h2><b>Today:</b><br><b>Plasmid preparation:</b><br>42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br><b>Transformation:</b><br>75. pEL3A15 - CrtB failed<br><br><b>PCR purification (of promotors):*</b><br>CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor constructs (not 77) from annealing of R and F (without PCR-cleaning).<br><br><b>Previous day:</b><br><b>ON screening:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></div>';
-'<h2>Ongoing constructs:</h2>'+
-'<b>L. reuteri:</b><br>'+
-'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>'+
-'8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>'+
-'10. 256-noplasmid<br>11. 36E-noplasmid<br><br>'+
-'<b>E. faecalis:</b><br>'+
-'9. JH2-2 pAMβ1<br><br>'+
-'<b>L. lactis:</b><br>'+
-'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
-'<b>E.coli (strain D5α):</b><br>'+
-'19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
-'23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>'+
-'76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
-'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
-'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+
-'<h2>Todays work</h2>'+
-'<b>PCR purification:</b><br>'+
-'CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br>'+
-'<b>Digestion:</b><br>'+
-'27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
-'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
-'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>'+
-'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br>'+
-'<b>Ligation:</b><br>'+
-'24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br>'+
-'<b>Transformation:</b><br>'+
-'75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br>'+
-'<b>Re-streak:</b><br>'+
-'1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'42. pSB4S15-red<br><br>'+
-'<b>O/N Screening PCR:</b><br>'+
-'25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
-'(19,20,21(with DMSO dilution series(7,9,12))<br><br>'+
-'<b>PCR (screening?):</b><br>'+
-'57.B0034-Idi<br>IspA<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
-'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>'+
-'27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br>'+
-'<h2>Results</h2>'+
-'<b>Today:</b><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>'+
-'42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>'+
-'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>'+
-'27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>'+
-'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br>'+
-'<b>Transformation:</b><br>'+
-'75. pEL3A15 - CrtB failed<br><br>'+
-'<b>PCR purification (of promotors):*</b><br>'+
-'CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>'+
-'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+
-'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+
-'constructs (not 77) from annealing of R and F (without PCR-cleaning).<br><br>'+
-'<b>Previous day:</b><br>'+
-'<b>ON screening:</b><br>'+
-'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
-'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
   }
 else if(id == 'd2013628')
   {
-  ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1><p><b>Name of participants: </b>'+
+	ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1><b>Name of participants: </b>Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W<br><br><h2>Ongoing constructs:</h2><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>ispA<br><br><h2>Todays work</h2><b>Frozen stock:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 2<br>Lb15. Lc. lactis unknown pGus clone 2<br>Lb16. Lc. lactis MG1363 noplasmid clone 2<br><br><b>Digest:</b><br>25.pSB1C3-CrtY<br><br><b>Transformation:</b><br>24.pSB1C3-CrtI<br>23.pSB1C3-CrtB<br><br><b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)”<br>Lb15. Lc. lactis MG1363 pGus clone 2 “I, II, III(2x1.5 ml culture)”<br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br><br><b>Re-streaks:</b><br> Lb3. reuteri DSM20016 pVS2<br>Lb8. reuteri 100-23 pGT232*<br>Lb11. plantarum 36E<br>Lb13. Lc. lactis MG1363 pJP059<br>Lb16. Lc. lactis MG1363 noplasmid<br>*from frozen stock<br><br><b>Streaking of strains:</b><br> Lb15. Lc. lactis MG1363 plasmid unknown pGus:<br>Lb18. Lb. delbrueckii bulgaricus (from freeze dried yoghurt culture) <br>Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) <br><br><b>O/N of restreak from Erik Gullberg:</b><br>42. pSB4S15-red, clones 1 and 2<br><br><b>PCR:</b><br>57.B0034-Idi*<br>ispA*<br>19.1**<br>20.1**<br>21.1**<br>* Amplified from D5α-chromosome. <br>** Now with DMSO<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi<br>ispA<br>19.1<br>20.1<br>21.1<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>O/N Screening:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br><br><h2>Results</h2><b>Previous day:</b><br><b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4)<br>→ Store in freezer, try again with new protocol <br><br><b>Transformation:</b><br> Lb10. plantarum 256 pLUL364: No growth<br>Lb11. plantarum 36E pVS2: No growth<br>Lb12. Lc. lactis DSM20016-pVS2: No growth<br>→ Try again later with new competent cells<br><br><b>Wednesday 26/6:</b><br><b>Streaking of strains:</b><br> Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br>Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with antibiotics<br>Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br><br><b>Today:</b><br> <b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “II”: 7,5 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl <br>Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ<br>Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl<br>Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl<br>→ Mark and store in freezer.<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi: hard do decipher results, redo tomorrow <br>ispA: (“)<br>19.1: failed<br>20.1: failed<br>21.1: failed<br>43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>46. pSB1C3-B0034-His-4CL: failed (“)<br>47. pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br><br><h2>Other experiments</h2><b>THMS buffer preparation</b><br><b>Aliquoting of sigma water</b><br><b>Preparation of antibiotic-free LB-agar plates</b><br><b>Dilution of promotor-oligonucleotides from stock to 5 µM:</b><br>CP1_F<br>CP1_R<br>CP11_F<br>CP11_R <br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F <br>CP30_R <br>CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br><b>Dilution of promotor oligonucleotide from pellet to 100 µM:</b><br>usp45_mut_F<br><br><b>Phosphorylation of promotor-oligonucleotides:</b><br>CP1_F <br>CP1_R <br>CP11_F <br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>CP29_F<br>CP29_R<br>CP41_F<br>CP41_R</div>';
-'Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M.,'+
-' Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W<br><br>'+
-'<h2>Ongoing constructs:</h2>'+
-'<b>L. reuteri:</b><br>'+
-'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>'+
-'6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>'+
-'14. DSM 20016-pLUL631(B?)<br><br>'+
-'<b>L. plantarum:</b><br>'+
-'5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br>'+
-'<b>L. lactis:</b><br>'+
-'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
-'<b>E.coli:</b><br>'+
-'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL'+
-'-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034'+
-'-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034'+
-'-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034'+
-'-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034'+
-'-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3'+
-'-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3'+
-'-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. '+
-'pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>ispA<br><br>'+
-'<h2>Todays work</h2>'+
-'<b>Frozen stock:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059 clone 2<br>Lb15. Lc. lactis unknown pGus clone 2<br>Lb16. Lc. lactis'+
-' MG1363 noplasmid clone 2<br><br>'+
-'<b>Digest:</b><br>'+
-'25.pSB1C3-CrtY<br><br>'+
-'<b>Transformation:</b><br>'+
-'24.pSB1C3-CrtI<br>23.pSB1C3-CrtB<br><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)”<br>Lb15. Lc. lactis MG1363 '+
-'pGus clone 2 “I, II, III(2x1.5 ml culture)”<br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>'+
-'7. pSB3K3-red<br>11. pSB4A15<br><br>'+
-'<b>Re-streaks:</b><br>'+
-'Lb3. reuteri DSM20016 pVS2<br>Lb8. reuteri 100-23 pGT232*<br>Lb11. plantarum 36E<br>Lb13. Lc. lactis'+
-' MG1363 pJP059<br>Lb16. Lc. lactis MG1363 noplasmid<br>*from frozen stock<br><br>'+
-'<b>Streaking of strains:</b><br>'+
-'Lb15. Lc. lactis MG1363 plasmid unknown pGus:<br>Lb18. Lb. delbrueckii bulgaricus (from freeze dried'+
-' yoghurt culture) <br>Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) <br><br>'+
-'<b>O/N of restreak from Erik Gullberg:</b><br>'+
-'42. pSB4S15-red, clones 1 and 2<br><br>'+
-'<b>PCR:</b><br>'+
-'57.B0034-Idi*<br>ispA*<br>19.1**<br>20.1**<br>21.1**<br>* Amplified from D5α-chromosome. <br>'+
-'** Now with DMSO<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi<br>ispA<br>19.1<br>20.1<br>21.1<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>'+
-'45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-'+
-'STS<br><br>'+
-'<b>O/N Screening:</b><br>'+
-'19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker'+
-'-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC'+
-'-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker'+
-'-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-'+
-'CrtB-B0034-CrtI-Linker-Zif268<br><br>'+
-'<h2>Results</h2>'+
-'<b>Previous day:</b><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4)<br>→ Store in freezer, try again '+
-'with new protocol <br><br>'+
-'<b>Transformation:</b><br>'+
-'Lb10. plantarum 256 pLUL364: No growth<br>Lb11. plantarum 36E pVS2: No growth<br>Lb12. Lc. lactis '+
-'DSM20016-pVS2: No growth<br>→ Try again later with new competent cells<br><br>'+
-'<b>Wednesday 26/6:</b><br>'+
-'<b>Streaking of strains:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br>'+
-'Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with '+
-'antibiotics<br>Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations '+
-'(1x, 5x & 10x) → Re-streak today<br><br>'+
-'<b>Today:</b><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 '+
-'“II”: 7,5 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl <br>'+
-'Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ<br>Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl'+
-'<br>Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl<br>→ Mark and store in freezer.<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi: hard do decipher results, redo tomorrow <br>ispA: (“)<br>19.1: failed<br>20.1: failed'+
-'<br>21.1: failed<br>43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: '+
-'failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>46. pSB1C3-B0034-His-4CL: failed (“)<br>47. '+
-'pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br><br>'+
-'<h2>Other experiments</h2>'+
-'<b>THMS buffer preparation</b><br>'+
-'<b>Aliquoting of sigma water</b><br>'+
-'<b>Preparation of antibiotic-free LB-agar plates</b><br>'+
-'<b>Dilution of promotor-oligonucleotides from stock to 5 µM:</b><br>'+
-'CP1_F<br>CP1_R<br>CP11_F<br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>'+
-'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br>'+
-'<b>Dilution of promotor oligonucleotide from pellet to 100 µM:</b><br>'+
-'usp45_mut_F<br><br>'+
-'<b>Phosphorylation of promotor-oligonucleotides:</b><br>'+
-'CP1_F<br>CP1_R<br>CP11_F<br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>'+
-'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br></p></div>';
   }
-   else if(id == 'd201373')
+else if(id == 'd2013627')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-27</h1><br><b>Name of participants: </b>Marcus H, Lovisa P, Emil M, Peter C, Ken B-A, Karl H, Kristoffer L, Christoffer A, Mikael S, Jens I, Nafisa B, Stephanie H, Alona N, Viktor T, Viktor B, Nils, Alexander, Thorsten, Pontus, Magnus, Malin<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>Lb11. plantarum 36E pVS2<br>Lb10. plantarum 256 pLUL634<br>Lb12. plantarum DSM20016 pVS2<br><br><b>Assembly:</b><br>23.Crt.B + pEL3A15<br>24.Crt.I + pEL3K16<br><br><b>Frozen stock: </b><br>Lb9. faecalis JH2-2 pAMβ1<br>Lb13. Lc. lactis MG1363 pJP059 clone 1<br>Lb15. Lc. lactis MG1363 pGus clone 1<br>Lb16. Lc. lactis MG1363 no plasmid clone 1<br><br><b>Plasmid preparation: </b><br>Lb13. Lc. lactis MG1363 pJP059 clone 1 <br>Lb15. Lc. lactis MG1363 pGus clone 1<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Over night:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19. (digest from previous day)<br>20. (“)<br>21. (“)<br><br><b>PCR amplification of construct:</b><br>miraculin<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>25. pSB1C3-CrtY<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>O/N screening PCR:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>25.pSB1C3-CrtY<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059 clone 2: Visual pellet, foggy medium; nc ok! * <br>Lb15. Lc. lactis MG1363 pGus clone 2: Visual pellet, foggy medium; nc ok! *<br>Lb16. Lc. lactis MG1363 no plasmid clone 2: Visual pellet, foggy medium; nc ok!*<br>Lb13. Lc. lactis MG1363 pJP059 clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>Lb15. Lc. lactis MG1363 pGus clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>Lb16. Lc. lactis MG1363 no plasmid clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>* Optimized conditions = 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. → Let grow for one more day <br>** → Frozen stock and plasmid prep.<br><br><b>Transformation: </b><br>42. pSB4S15-red: Many small white colonies, contamination with cocci?<br>→ Discard, get clones from Erik G.<br><br><b>Streaking of strains:</b><br>Lb13. Lc. lactis MG1363 pJP059: Some growth.<br>Lb15. Lc. lactis MG1363 pGus: Hard to see without opening lock.<br>Lb16. Lc. lactis MG1363 no plasmid: Hard to see without opening lock.<br>→ let grow one more day.<br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>Lb9. faecalis JH2-2 pAMβ1 clone 1: 10.1 ng/µl<br>Lb9. faecalis JH2-2 pAMβ1 clone 2: 24.7 ng/µl<br>Lb13. Lc. lactis  MG1363-pJP059 clone 1: 4.9 ng/µl (run 1)<br>Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.0 ng/µl (run 2)<br>Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.1 ng/µl (run 3)<br>Lb15. Lc. lactis MG1363 pGus clone 1: 61 ng/µl (run 1)<br>→ Measure again tomorrow<br><br><b>Gel electrophoresis:</b><br>Miraculin: fail, problems with primers, probably due to problematic secondary structure.<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: failed, ladder too, probably problem with gel. Image 1 (2013.06.27)<br>19. (digest from previous day): Success<br>20. (“): Success<br>21. (“): Success<br>19. (PCR): Fail -> redo with lower TM<br>20. (“): Fail -> redo with lower TM<br>21. (“): Fail -> redo with lower TM<br>25. pSB1C3-CrtY: Good results<br><br><h2>Other experiments</h2><br><b>Preparation of THMS buffer</b></div>'
+ }
+else if(id == 'd2013626')
+ {
+	ds = '<div id="dairy-text"><h2>Wednesday 2013-06-26</h2><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Anders E, Mikael S, Anton B, Viktor B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Theodor L, Magnus B., Malin B., Thorsteinn O., Nils A., Alexander W., Niclas S., Pontus D., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>25.pSB1C3-CrtY<br>19.psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>20.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>21.psB4C5-CrtE~I / w. linker, ZF & RBS<br>42. D5α with plasmid pSB4S15-red<br><br><h2>Todays work</h2><br><b>Competent cells preparation:</b><br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Transformation:</b><br>plantarum 36E pAMB1<br>plantarum 256 pAMB1<br>Lb3. reuteri DSM 20016 pVS2<br>25.pSB1C3-CrtY<br>42. pSB4S15-red <br><br><b>O/N:</b><br>Lb5.2 plantarum 256 pAMβ1*<br>Lb8.2 reuteri 100-23 pGT232*<br>Lb13. Lc. lactis MG1363 pJP059**<br>Lb15. Lc. lactis MG1363 pGus**<br>Lb16. Lc. lactis MG1363 no plasmid**<br>*Two tubes of 8.2 were prepared. It has grown rather poorly to not at all before. If it continues to grow poorly we may have to redo O/N from a plate rather than from our frozen stock.<br>** Optimized conditions → 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved.  <br><br><b>Digestion:</b><br>19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>21.1.psB4C5-CrtE~I / w. linker, ZF & RBS<br>21.2.psB4C5-CrtE~I / w. linker, ZF & RBS<br><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br><br><b>PCR amplification of construct:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (third try, using same concentration of template as second try but lower annealing temperature )<br>19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>21.1.psB4C5-CrtE~I / w. linker, ZF & RBS<br>21.2.psB4C5-CrtE~I / w. linker, ZF & RBS<br><br><br><h2>Results</h2><br><b>Previous day:</b><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059: Growing, visual pellet.*<br>Lb15. Lc. lactis MG1363 pGus: Growing, visual pellet.*<br>Lb16. Lc. lactis MG1363 no plasmid: Growing, visual pellet.*<br>*Weak growth in the morning, better in the afternoon. → Let grow for one more day + new O/N with optimized conditions.<br><br><b>Streaking of strains: </b><br>Lb13. Lc. lactis MG1363 pJP059: No visual growth → Let grow for one more day.<br>Lb15. Lc. lactis MG1363 pGus: No visual growth →		--”--<br>Lb16. Lc. lactis MG1363 no plasmid: No visual growth → 	--”—<br><br><b>Today:</b><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: Failed, possibly nonspecific primer binding, not enough template DNA. Image 1 (2013.06.26)<br><br><b>Transformation:</b><br>25.pSB1C3-CrtY: failed<br>D5alpha competent cells were competent<br><br><br><h2>Other experiments</h2><br><b>Preparation of cold electroporation buffer for competent cells</b><br><br><b>Preparation of 50% glycerol stock: </b><br>500 ml 50% glycerol stock was prepared from 86-88% glycerol.</div>'
+ }
+ else if(id == 'd2013625')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-06-25</h1><br><br><b>Name of participants:</b> Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Theodor L, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><h2>Todays work</h2><br><b>Gel electrophoresis:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>PCR amplification of construct:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (second try, using smaller concentration of template and higher annealing temperature )<br>Lb13. Lc. lactis MG1363 pJP059<br><br><b>Competent cells preparation: </b><br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 noplasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Plasmid preparation: </b><br>Lb6.1 reuteri DSM 20016 pLUL634TsA8<br>Lb6.2 reuteri DSM 20016 pLUL634TsA8<br>Lb6.3 reuteri DSM 20016 pLUL634TsA8<br>Lb6.4 reuteri DSM 20016 pLUL634TsA8 *<br>Lb7.2 reuteri DSM 20016 pGFP<br>Lb7.3 reuteri DSM 20016 pGFP<br>Lb7.4 reuteri DSM 20016 pGFP *<br>Lb14.1 reuteri DSM 20016 pLUL631<br>Lb14.2 reuteri DSM 20016 pLUL631<br>Lb14.3 reuteri DSM 20016 pLUL631<br>Lb14.4 reuteri DSM 20016 pLUL631 *<br>* 6.4, 7.4 and 14.4 were treated in a different way than the regular plasmid prep protocol for L. reuteri. Instead of treating them with LYSIS-buffer and lysozyme they were treated with resuspension solution and lysis solution from the plasmid prep kit and allowed to lyse for ~30 minutes. The rest were treated as usual, but with 1 ml of 10 mg/ml lysozyme instead of 2 ml.<br><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059<br>Lb15. Lc. lactis MG1363 pGus<br>Lb16. Lc. lactis MG1363 no plasmid<br><br><b>Streaking of strains:</b><br>Lb13. Lc. lactis MG1363 pJP059<br>Lb15. Lc. lactis MG1363 pGus<br>Lb16. Lc. lactis MG1363 no plasmid<br><br><b>PCR amplification: </b><br>Lb13. Lc. lactis MG1363 pJP059*<br>*Another attempt at colony PCR with different concentrations of lysozyme and buffer.<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>15. failed. Possibly nonspecific primer binding, too much template DNA. Image 1 (2013.06.25)<br>Lb13. Lc. lactis MG1363 pJP059: Only short bands, probably primer dimers because lysing failed. → Try again with a plasmid prep instead of culture.<br><br><b>Competant cells:</b><br>Wrong volume was added. → Try again tomorrow with the right volume.<br><br><b>Plasmid preparation: *</b><br>Lb6.1 reuteri DSM 20016 pLUL634 TsA8:	203.7 ng/µl<br>Lb6.2 reuteri DSM 20016 pLUL634 TsA8:	203.2 ng/µl<br>Lb6.3 reuteri DSM 20016 pLUL634 TsA8:	154.8 ng/µl<br>Lb6.4 reuteri DSM 20016 pLUL634 TsA8:	39.5 ng/µl<br>Lb7.2 reuteri DSM 20016 pGFP:		100.5 ng/µl<br>Lb7.3 reuteri DSM 20016 pGFP:		141.2 ng/µl<br>Lb7.4 reuteri DSM 20016 pGFP:		8.7 ng/µl<br>Lb14.1 reuteri DSM 20016 pLUL631:	162.7 ng/µl<br>Lb14.2 reuteri DSM 20016 pLUL631:	85.2 ng/µl<br>Lb14.3 reuteri DSM 20016 pLUL631:	49.8 ng/µl<br>Lb14.4 reuteri DSM 20016 pLUL631:	7.7 ng/µl<br>*The alternative way of lysing the cells proved to be quite ineffective so we will continue to use our standard protocol. The earlier plasmid preparations of the above “constructs”, that had far poorer concentrations, were replaced with these better ones.<br><br><h2>Other experiments</h2><br><b>Preparation of MRS broth </b><br><br><b>Making plates for Lc. lactis cultivation (from M17-broth)</b><br><br><b>Preparation of homemade yogurt from dried bacteria and from commercial yogurt with active bacteria culture</b></div>'
+ }
+ else if(id == 'd2013624')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1><br><b>Name of participants: </b>Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D.<br><br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m	<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>Lb8. reuteri 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Digestion of construct:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>O/N (from frozen stock): </b><br>Lb2. 1063 pLUL631<br>Lb6. DSM 20016 pLUL63TsA8<br>Lb7. DSM 20016 pGFP<br>Lb8. 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>PCR:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT*<br>Lb13. Lc. lactis MG1363 pJP059*<br>*Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. <br><br><b>Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: </b><br>1.1. pSB1C3-red<br>18.2. pSB1C3-CHS_m<br>40. pEL3A17-red<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br>Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl<br>Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl<br>Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today.<br><br><h2>Other experiments</h2><br><b>Primer stock preparation</b><br><br><b>Dilution of sequencing primers: </b><br>3 tubes of 5 µM VF2-primer, 200 uL<br>3 tubes 5 µM VR-primer, 200 µL<br><br><b>Re-measuring concentration of earlier plasmid preps: *</b><br>1.4 CF48-pLR581: 127.8 ng/µl<br>1.5 CF48-pLR581: 215.9 ng/µl<br>3.2 DSM 20016-pVs2: 201.2 ng/µl<br>3.3 DSM 20016-pVs2: 139.1 ng/µl<br>3.10 DSM 20016-pVs2: 183.5 ng/µl<br>*We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind.<br><br><b>Preparing M17-broth, 950 ml.</b><br><b>(“) SOB</b><br><b>(“) CCMB80 (20% glycerol, pH 6,8)</b><br><b>Alliquoting dNTP, buffer, enzymes</b></div>'
+ }
+   else if(id == 'd2013623')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1><br><b>Name of participants: </b>Hampus E, Alexander W<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>4. 100-23-noplasmid<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>E. coli: </b><br>23.pCrcSB1C3-Crt-B<br>24.pSB1C3-Crt-I<br>25. pSB1C3-CrtY<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb5. plantarum 256 rifR-Pamβ1<br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><b>Restreak:</b><br>25. pSB1C3- crtY<br> <br><b>Plasmidpreparation </b><br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><h2>Result</h2><br><b>O/N:</b><br>25.pSB1C3-crtY: One of the overnights did not grow<br></div>'
+ }
+ else if(id == 'd2013622')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-06-22</h1><br><b>Name of participants: </b>Nafisa B, Mikael S, Marcus H<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation: </b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red <br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb8. reuteri 100-23-pGT232<br>Lb9. faecalis  JH2-2- pAMβ1<br>Lb10. plantarum 256-noplasmid<br>Lb11. plantarum 36E-noplasmid<br>Lb12. reuteri DSM 20016-noplasmid<br>Lb14. reuteri DSM 20016-pLUL631 (B?)<br><br><b>O/N: </b><br>Lb4. reuteri100-23-noplasmid<br>Lb5. plantarum 256-rifR-pAMβ1<br><br><b>Plasmid preparation:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red <br>Lb14. DSM 20016-pLUL631 (B?)<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br><b>L. reuteri:</b><br>Lb14.1 (strain 14, clone 1) DSM 20016-pLUL631 (B?): 10.0 ng/μl<br>Lb14.2 DSM 20016-pLUL631 (B?): 13.6 ng/μl<br>Lb14.3 DSM 20016-pLUL631 (B?): 14.7 ng/μl<br>Lb14.4 DSM 20016-pLUL631 (B?): 28,4 ng/μl<br>Lb6.1 DSM 20016-pLUL63TsA8: 18.7 ng/μl<br>Lb6.2 DSM 20016-pLUL63TsA8: 14.4 ng/μl<br>Lb6.3 DSM 20016-pLUL63TsA8: 12.5 ng/μl<br>Lb6.4 DSM 20016-pLUL63TsA8: 27.7 ng/μl<br>Lb7.2 DSM 20016-pGFP: 29.9 ng/μl<br>Lb7.3 DSM 20016-pGFP: 16.9 ng/μl<br>Lb7.4 DSM 20016-pGFP: 10.9 ng/μl</div>'
+ }
+   else if(id == 'd2013621')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-06-21</h1><br><b>Name of participants: </b>Stephanie H, Alona N, Anton B, Mikael S, Nafisa B, Anders E, Marcus H, Ken B-A, <br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work</h2><br><b>Plasmid preparation*: </b><br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><i>*to get more useable material and to look for different result from clones grown in a lock and a CO2-cupboard.</i><br><br><b>O/N: </b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb12. reuteri DSM 20016-noplasmid<br>Lb8. reuteri 100-23-pGT232<br>Lb10. plantarum 256-noplasmid<br>Lb11. plantarum 36E-noplasmid<br>Lb9. faecalis JH2-2 pAMβ1 *-<br><i>*from the plate without erythromycin. </i><br><br><b>Plasmid preparation:</b><br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>Re-streak: </b><br>Lb4. reuteri 100-23-noplasmid: No growth. → Grow on non-antibiotic plates  <br><br><b>Today:</b><br><b>Plasmid preparation:</b><br>Lb1.2 (strain 1, clone 2) reuteri CF48-pLR 581: Non-usable concentration *<br>Lb1.4 reuteri CF48-pLR 581: 161.8 ng/µl *<br>Lb3.8 reuteri DSM 20016 pVS2: 148.5 ng/µl *<br>Lb3.10 reuteri DSM 20016 pVS2: Non-usable concentration *<br><i>*there is seemingly no correlation between growth in lock vs. CO2-cupboard and plasmid concentration. This seems strange though. Cupboard is not supposed to create an anaerobic environment → do not use cupboard</i><br><br><h2>Other experiments</h2><br><b>SET buffer preparation: </b><br><b>Lysis buffer preparation: </b></div>'
+ }
+ else if(id == 'd2013620')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S, Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br><br><br><h2>Todays work:</h2><br><b>Glycerol stock preparation: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>Plasmid preparation:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>26. pEL3K15-red<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>O/N: </b><br>Lb2. reuteri 1063-pLUL631<br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><br><b>Streaking of new strains from Stefan Roos: </b><br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb12. reuteri DSM 20016-noplasmid *<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb4. reuteri 100-23-noplasmid<br>Lb8. reuteri 100-23-pGT232 *<br>Lb5. plantarum 256-rifR-pAMβ1<br>Lb10. plantarum 256-noplasmid *<br>Lb11. plantarum 36E-noplasmid *<br>Lb9. faecalis JH2-2 pAMβ1 **<br><i>*both on burned broth without glucose and CaCl2 and regular one.</i><br><i>**re-streaked on erythromycin and on antibiotic-free plates.</i><br><br><b>Transformation:</b><br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br><br><b>Restreaks:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br><br><h2>Results</h2><br><b>Previous day:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17)   <br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>19.1: 50.5 ng/µl  <br>19.2: 33.6 ng/µl<br>19.3: 45.2 ng/µl<br>19.4: 36.5 ng/µl<br>20.1: 28.9 ng/µl<br>20.2: 29.6 ng/µl<br>20.3: 41.8 ng/µl<br>20.4: 35.8 ng/µl<br>21.1: 47.7 ng/µl<br>21.2: 23.2 ng/µl<br>21.3: 38.8 ng/µl<br>21.4: 48.9 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again.<br>Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again.<br>Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl<br><br><br><h2>Other experiments</h2><br><b>Antibiotic-free MRS-broth plates preparation: </b><br><b>SET-buffer and Lysis-buffer preparation: </b><br><b>MRS-broth agar preparation: </b><br><b>Preparation of plates with and without antibiotic:</b> </div>'
+ }
+   else if(id == 'd2013619')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-06-19</h1><br><br><b>Name of participants: </b>Emil M, Marcus H, Ken B-A, Sabri J., Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B, Malin B, Pontus D, Magnus B, Alexander W, Thorsteinn O, Niclas S, Nils A<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br><br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>25. pSB1C3-CrtY<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red  <br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM20016-pVs2<br><br><b>Spreading:</b> <br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>O/N:</b> <br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM20016-pVs2<br><br><b>Transformation:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>25. pSB1C3-CrtY<br><br><h2>Results</h2><br><b>Previous day: </b><br><b>Spreading:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth. → Try growing with less oxygen. <br><br><b>Transformation</b><br>1. pSB1C3-red: Successful<br>2. pSB3C5-red: Successful<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: Successful<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br><br><b>Restreak:</b><br>16. pSB1C3-TAL_m: Successful<br>17. pSB1C3-4Cl_m: Successful<br>18. pSB1C3-CHS_m: Successful<br>22. pSB1C3-B0032-BFP: Successful<br><br><b>Today:</b><br><b>O/N: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><b>Transformation:</b><br>23. pSB1C3-CrtB: fail<br>24. pSB1C3-CrtI: fail<br>25. pSB1C3-CrtY: fail<br><br><h2>Other experiments</h2><br><b>LB-agar plates </b><br><b>CaCl2-solution</b></div>'
+ }
+ else if(id == 'd2013618')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-06-18</h1><br><b>Name of participants: </b>Malin B, Pontus D, Magnus B, Alexander V, Thorsteinn O, Niclas S, Nils A, Christoffer F, Kristoffer L, Emil M, Theodor L, Marcus H, Ken B.-A, Peter C, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br> <br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059   <br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work:</h2><br><b>Streaking of strains from Stefan Roos: </b><br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631 <br>Lb3. reuteri DSM 20016-pVS2<br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><b>Restreak</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Previous day</b><br><b>Restreak:</b><br>14. pET13b-zifz:4cl-PBSII:STS: Growth<br><br><b>Today</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: fail<br>16. pSB1C3-TAL_m: successful<br>17. pSB1C3-4Cl_m: successful<br>18. pSB1C3-CHS_m: successful<br>22. pSB1C3-B0032-BFP: successful<br><br><b>Restreak:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><h2>Other experiments:</h2><br><b>LB-agar</b></div>'
+ }
+else if(id == 'd2013617')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-17</h1><br><b>Name of participants: </b>Kristoffer L, Emil M, Lovisa P, Theodor L, Marcus H, Ken B-A, Peter C, Christoffer A, Anton B, Nafisa B, Stephanie H, Alona N, Anders E, Malin B., Pontus D., Alexander V., Magnus B., Nils A. Thorsteinn O., Christoffer F<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Today:</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>14. pET13b-zifz:4cl-PBSII:STS: successful<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m: fail<br>17. pSB1C3-4Cl_m: fail<br>18. pSB1C3-CHS_m: fail<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: successful<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: successful<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: successful<br><br><h2>Other experiments:</h2><br><b>Mixing MRS-broth</b><br><b>Making of EDTA solution</b><br><b>MRS-plates preparation</b></div>'
+ }
+ else if(id == 'd2013613')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-13</h1><br><b>Name of participants:</b> Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F.<br><br><h2>Other experiments</h2><br><b>Making competent D5α</b></div>'
+ }
+   else if(id == 'd2013612')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-06-12</h1><br><b>Name of participants: </b>Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F., Pontus D<br><br><h2>Other experiments</h2><br><b>LB-agar plates</b></div>'
+ }
+   else if(id == 'd2013610')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-10</h1><br><b>Name of participants: </b>Alexander W, Magnus B, Malin B, Thorsteinn O, Nils A, Christoffer F., Pontus D, Niclas S.<br><br><h2>Other experiments:</h2><br><b>LB</b><br><b>CaCl2</b><br><b>Antibiotics</b></div>'
+ }
+ else if(id == 'd201373')
   {
    ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1><br><b>Name of participants:</b> Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A,  Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>1. pSB1C3-red<br>7. pSB3K3-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><br><b>L. plantarum:</b><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br><br><br><b>L. reuteri:</b><br>Lb12. DSM 20016-noplasmid<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br><br><h2>Todays work</h2><br><b>PCR:</b><br>Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	  (colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	  (colony)*<br><i>*Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br>Lb13.2.2 lactis MG1363 pJP059	(plasmid)**<br>Lb13.2.3 lactis MG1363 pJP059	(plasmid)**<br>Lb13.3 lactis MG1363 pJP059		(colony)**<br>Lb13.4 Lc. lactis MG1363 pJP059	(colony)**<br><i>**Gel will be done tomorrow. Followed the PCR protocol with DMSO.</i><br>78. pEL3C18-CrtY*<br><i>-*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time)</i><br><br>Digest:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Ligation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>1. pSB1C3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>7. pSB3K3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><i>-*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning.</i><br><br><b>Transformation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77 pSB4C15-red<br><br><b>O/N:</b><br>Lb10.1. plantarum 256 no plasmid<br>Lb11.1. plantarum 36E no plasmid <br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br><br><b>Plasmid preparation:</b><br>7.pSB3K3<br>22.pSB1C3-B0032-BFP<br>42. pSB4S15-red<br><br><b>Gelelectrophoresis:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br>Lb13.2.3 Lc. lactis MG1363-pJP059*<br>Lb13.3 Lc. lactis MG1363-pJP059*<br>Lb13.4 Lc. lactis MG1363-pJP059*<br><i>*Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br><br><b>Results</b><br><b>Gelelectrophoresis:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><i>-Digestion Worked</i><br><br><b>failed:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	(colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	(colony)*<br><i>-*The gel showed nothing but primer dimer.</i><br><br><b>Digest:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Yesterdays transformation:</b><br><b>successful:</b><br>61. pSB1C3-CP1: Growth<br>61. pSB1C3-CP1 10x: Some growth<br>62. pSB1C3-CP8: Some growth<br>62. pSB1C3-CP8 10x: No growth<br>63. pSB1C3-CP11: Some growth<br>63. pSB1C3-CP11 10x: No growth<br>64. pSB1C3-CP29: Some growth<br>64. pSB1C3-CP29 10x: No growth<br>65. pSB1C3-CP30: Some growth<br>65. pSB1C3-CP30 10x: No growth<br>66. pSB1C3-CP41: Growth<br>66. pSB1C3-CP41 10x: Some growth<br>67. pSB1C3-Cp44: No growth<br>67. pSB1C3-Cp44 10x: No growth<br>68. pSB3K3-CP1-B0032-BFP: No growth<br>68. pSB3K3-CP1-B0032-BFP 10x: Growth<br>69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated<br>69. pSB3K3-CP8-B0032-BFP 10x: No growth<br>70. pSB3K3-CP11-B0032-BFP: Growth<br>70. pSB3K3-CP11-B0032-BFP 10x:  Growth<br>71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies<br>71. pSB3K3-CP29-B0032-BFP 10x: -----<br>72. pSB3K3-CP30-B0032-BFP: Growth<br>72. pSB3K3-CP30-B0032-BFP 10x: No growth<br>73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies<br>73. pSB3K3-CP41-B0032-BFP 10x: No growth<br>77.1 pSB4C15, clone 1: No growth<br>77.1 pSB4C15, clone 1 10x: No growth<br>77.2 pSB4C15, clone 2: Some  growth<br>77.2 pSB4C15, clone 2 10x: No growth<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>27. pEL3K16-red<br>38. pEL3A15-red<br>51. pEL3C18-red<br><br><b>failed:</b><br>81. pSB4S15-CP41: No growth<br>82. pSB4S15-CP29: No growth<br>83. pSB4S15-CP30: No growth<br><i>→ Redo ligation and transformation.</i><br><br><b>Plasmid preparation:</b><br>7.3  pSB3K3 clone 3: 50.1 ng/µl<br>7. 4 pSB3K3 clone 4: 43.4 ng/µl<br>7. 5 pSB3K3 clone 5: 48.1 ng/µl<br>22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl<br>22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ<br>22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ<br>42.1 pSB4S15-red clone 1: 43.6 ng/µl<br>42.2 pSB4S15-red clone 2: 46.1 ng/µl<br><br><h2>Other experiments</h2><br>Made agarplates with chloramphenicol resistance<br>MRS-broth preparation<br>MRS-broth-agar preparation <br>MRS-broth-agar plates preparation </div>';
@@ Line 543: / Line 424: @@
    '<h2>Other experiments</h2><br>-<br></p></div>';
   }
+   else if(id == 'd201382')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-02</h1><br><b>Name of participants: </b>Anton B, Christoffer A, Mikael S, Jens I, Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E, Magnus, Pontus, Sabri, Nils<br><br><h2>Ongoing constructs:</h2><br><b>E.coli:</b><br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>108. pSBA15-J23106-B0034-IspA<br>109. pSBA15-K206000-B0034-IspA<br>110. pSBA15-K206001-B0034-IspA<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>140. pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141. pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142. pSB3T5-CP1-B0034-UGTCs2-6xHis<br>163. pEL3S17-ZCD-His-B0034-UGTCs2-His<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>177. pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178. pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183. pEL3A15-B0034-CrtZ<br>184. pSB1C3-B0034-CrtZ<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>191. pSB1A2-fusionGFP<br>193. pSB1C3-amilGFP<br>194. pSB1C3-B0034-amilCP<br>195. pSB1C3-cjBlue<br>196. pSB1C3-eforRed<br>197. pSB1C3-aeBlue<br>198. pSB1C3-B0034-amilGFP<br>199. pSb1C3-amajCFP<br>200. pSB1C3-B0034-asCP<br>202. pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>204. pEL3S17-J23110-HIS-STS<br>205. pSBLB4C15-CP1-B0034-HIS-TAL<br>206. pSB1C3-J23101-B0034-TAL<br>207. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br><br><b>L. reuteri:</b><br>3. DSM 20016 pVS2<br>4. 100-23 no plasmid<br>12. DSM 20016 no plasmid<br>23. 100-23 pSBLb4C15-red<br>24. DSM 20016 pSBLb4C15-red<br>26. 100-23 pSBLbEcC2-red<br>27. DSM 20016 pSBLbEcC2-red<br>28. 100-23 pVS2<br><br><h2>Todays work</h2><br><b>Plasmid preparation: </b><br>139.1.1 pSB3K3-J23101-B0032-BFP <br>139.1.2 pSB3K3-J23101-B0032-BFP<br>191.1 pSB1A2-fusionGFP<br>191.2 pSB1A2-fusionGFP<br><br><b>Ligation:</b><br>4.pSB1A3-Red, 147.pSB1C3-B0034-Thioredoxin, 125.pSB1C3-ZCD-His-><br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br><br><b>Streaking of plasmid-free cells from frozen stock as control of C-resistance: </b><br>Lb4.1 reuteri. 100-23 no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak of construct:</b><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br><b>Screening:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Overnight:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Gel electrophoresis:</b><br>PCR of 152. pUC57-B0034-Exp-Linker-Miraculin-Linker-His to extract Miraculin with biobrick prefix and suffix<br><br><h2>Results</h2><br><b>Today:</b><br><b>Plasmid preparation:</b> <br>139.1.1 pSB3K3-J23101-B0032-BFP: 51.9 ng/µl <br>139.1.2 pSB3K3-J23101-B0032-BFP: 53.6 ng/µl<br>191.1 pSB1A2-fusionGFP: 78.9 ng/µl<br>191.2 pSB1A2-fusionGFP: 74.1 ng/µl</div>'
+ }
+    else if(id == 'd201383')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>'
+ }
+    else if(id == 'd201383')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>'
+ }
+     else if(id == 'd201384')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-04</h1><br><b>Name of participants: </b>Emil M, Magnus, Pontus<br><br><h2>Ongoing constructs:</h2><br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br><br><h2>Todays work:</h2><br><b>Overnight:</b><br>10. D5α<br><br><h2>Characterization</h2><br><b>Detection of TAL activity through spectroscopy (day 1)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2Other experiments:</b><br><b>Competent cell test</b><br><br></div>'
+ }
+     else if(id == 'd201385')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-05</h1><br><b>Name of participants: </b>Anton B, Viktor T, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri:</b><br>23. 100-23 pSBLb4C15-red<br>25. 100-23 pSBLbC2-red<br>28. 100-23 pVS2<br><br><b>E. coli:</b><br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Todays work</h2><br><b>Competent cells</b><br>Lb4.1 reuteri 100-23 no plasmid<br><br><b>Lambda red transformation:</b><br>Crt<br><br><b>Transformation:</b><br>reuteri 100-23 pSB1C3-red (1 tube, to see if it would work in Lb, died in electroporation)<br>Lb23. reuteri 100-23 pSBLb4C15-red (5 tubes, 1 died in electroporation)<br>Lb25. reuteri 100-23 pSBLbC2-red (1 tube)<br>Lb28. reuteri 100-23 pVS2 (2 tubes, 1 died in electroporation)<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Screening:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br><br><h2>Characterization</h2><br><b>Detection of TAL activity through spectroscopy (day 2)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2>Other experiments</h2><br><b>Casting LB-agar plates: </b><br>Kanamycin, 63 plates<br>Chloramphenicol, 61 plates<br>Spectinomycin, 65 plates<br>Tetracycline, 32 plates<br>Ampicillin, 30 plates<br><br><b>Competent cells (D5α)</b></div>'
+ }
+      else if(id == 'd201386')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-06</h1><br><b>Name of participants:</b> Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Anton B, Mikael S, Viktor T<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>57.3.pSB1C3-B0034-Idi<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>6. pSB1K3-red<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>111. pSBLb4C15-red<br>139. pSB3K3-J23101-B0032-BFP<br>165. pSB1C3-RBS-tsPurle<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>194. pSB1C3-B0034-amilCP<br>198. pSB1C3-B0034-amilGFP<br><br><b>L. reuteri:</b><br>23. 100-23 pSBLb4C15-red<br>25. 100-23 pSBLbC2-red<br>28. 100-23 pVS2 <br><br><h2>Todays work:</h2><br><b>Transformation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Restreak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>115. pSB3K5-J23101-B0034-His-TAL<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Digest:</b><br>6. pSB1K3-red<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>68.2 pSB3K3-CP1-B0032-BFP (EcoRI + PstI)<br>69.4 pSB3K3-CP8-B0032-BFP (EcoRI + PstI)<br>70.3 pSB3K3-CP11-B0032-BFP (EcoRI + PstI)<br>71.1 pSB3K3-CP29-B0032-BFP (EcoRI + PstI)<br>72.2 pSB3K3-CP30-B0032-BFP (EcoRI + PstI)<br>73.4 pSB3K3-CP41-B0032-BFP (EcoRI + PstI)<br>74. pSB3K3-CP44-B0032-BFP (EcoRI + PstI)<br>111. pSBLb4C15-red (XbaI + PstI)<br>165. pSB1C3-RBS-tsPurle (XbaI + PstI)<br>166. pSB1C3-RBS-scOrange (XbaI + PstI)<br>167. pSB1C3-RBS-fwYellow (XbaI + PstI)<br>194. pSB1C3-B0034-amilCP (XbaI + PstI)<br>198. pSB1C3-B0034-amilGFP (XbaI + PstI)<br><br><b>Ligation:</b><br>B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin<br>B0034-Thioredoxin, 1. pSB1C3-red (X, P) -> 147. pSB1C3-B0034-Thioredoxin<br><br><b>Gel: </b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br><br><b>Screening:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br>204.(1-4) pEL3S17-J23110-B0034-HIS-STS<br>171(1-4) pEL3S17-J23110-B0034-4CL-B0034-STS<br><br><b>Mutagenesis:</b><br>43.7  pSB1C3-B0034-TAL<br>57.3.pSB1C3-B0034-Idi -> 129.pSB1C3-Idi(mut)<br><br><v>Sequencing preparation:</b><br>139.1 pSB3K3-J23101-B0032-BFP<br>185.9 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>185.10 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>O/N:</b><br>204.2 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br>111.7 pSBLb4C15-red<br><br><h2>Results:</h2><br><b>Gel:</b> <br>164.E.Coli Nissle-CrtY (clones from transformation plate) failed<br>201.E.Coli Nissle-CrtY-CrtI failed<br><br><b>Previous day:</b><br><b>Transformation: </b><br>No visual growth. Keeping them on growth for one more day.<br><br><h2>Characterization:</h2><br><b>Detection of TAL activity through spectroscopy (day 3)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2>Other Experiments:</h2><br>10.D5Alpha competence test<br><br><b>Preparation of buffers for Western Blot</b><br><br><b>Casting LB-agar plates:</b><br>Kanamycin, 33 plates<br>Spectinomycin, 31 plates<br><br></div>'
+ }
+      else if(id == 'd201387')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-07</h1><br><b>Name of participants: </b>- Viktor T, Anton B, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>6. pSB1K3-red<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>92. J23110<br>111. pSBLb4C15-red<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>240. pSB1K3-J23110-RBS-tsPurple<br>241. pSB1K3-J23110-RBS-scOrange<br>242. pSB1K3-J23110-RBS-fwYellow<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br><br><h2>Todays work</h2><br><b>Assembly:</b><br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>240. pSB1K3-J23110-RBS-tsPurple*<br>241. pSB1K3-J23110-RBS-scOrange*<br>242. pSB1K3-J23110-RBS-fwYellow*<br><i>*Will eventually be tried in shuttle vector, but that has C-resistance, so a subcloning to K is necessary first, or a modification to change the resistance of the shuttle vector.</i><br><br><b>Transformation:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>240. pSB1K3-J23110-RBS-tsPurple<br>241. pSB1K3-J23110-RBS-scOrange<br>242. pSB1K3-J23110-RBS-fwYellow<br>108.pEL3S17-J23106-B0034-IspA<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br><br><b>Spreading: </b><br>60. PK401 dam- no plasmid<br>111.7 pSBLb4C15-red<br><br><b>Restreak:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><b>O/N & Screening:</b><br>110.pEL3S17-K206001-B0034-IspA<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pBAD/AraC-RBS-Luciferas (2012 years kit)<br>227.pBAD/AraC-RBS-Luciferas (2013 years kit)<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br><br><b>Gel:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br>129.pSB1C3-Idi(mut)<br><br><h2>Results</h2><br><b>Transformation (Monday):</b><br>Still no visual growth. <br><br><b>SDS-page failed (probably due to faulty buffers, investigate the running buffer first)</b><br><br><h2>Characterization experiments</h2><br><b>SDS-page on constructs:</b><br>43.7 pSB1C3-B0034-TAL<br><br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><h2>Other experiments</h2><br><b>Sequencing preparation:</b><br>69. pSB3K3-CP8-B0032-BFP*<br><i>*since the existing sequencing data doesn’t cover the whole insert with sufficient accuracy. There may be a mutation at the end of BFP, but that is more than 800bp after the single primer. Was not sent today, not sequencing tuesday.</i></div>'
+ }
+      else if(id == 'd201388')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-08</h1><br><b>Name of participants:</b> Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Stephanie H, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>60. Strain PK401 dam- no plasmid<br>111.7 pSBLb4C15-red<br>139. pSB3K3-J23101.B0032-BFP<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br><br><h2>Todays work:</h2><br><b>Transformation:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>no nr. pSB1c3-Thioredoxin<br>no nr. pEL3S17-Thioredoxin<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><b>Re-streak:</b><br>108.pEL3S17-J23106-B0034-IspA<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><bPlasmid prepp</b><br>226. pBAD/AraC-RBS-Luciferas (2012 years kit)<br>227.pBAD/AraC-RBS-Luciferas (2013 years kit)<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br><br><b>O/N screening:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Two step Assembly</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br><br><b>Overnight (screening)</b><br>1. pSB1C3-red<br><br><b>O/N</b><br>60. Strain PK401 dam- no plasmid<br>111.7 pSBLb4C15-red<br><br><b>Streak from frozen stock</b><br>3. pSB3T5-red<br>6. pSB1K3-red<br>42. pSB4S15-red<br>77. pSB4C15-red<br><br><h2>Results:</h2><br><b>One of the SDS-gels failed, resulting in no good gel to stain (faulty buffers, continue to search for sources of errors)</b><br><br><b>The succesfull gel was prepared with control buffers.</b><br><br><b>Transformation of promotors with BFP in the shuttle vector, for characterization in Lactobacillus: Some growth, only white colonies, will let them grow overnight again. </b><br><br><h2>Characterization experiments:</h2><br><b>Westernblot </b><br><b>SDS-page on constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><b>Electro transfer over night</b><br><br><b>Staining of control gel</b><br><br><b>Detection of TAL activity through spectroscopy (day 4)</b></div>'
+ }
+      else if(id == 'd201389')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-09</h1><br><b>Name of participants:</b> Stephanie H, Mikael S, Anton B, Anders E, Magnus, Pontus, Sabri, Malin, Alexander, Nils, Christoffer<br><br><h2>Ongoing constructs:</h2><br>60. PK401 dam- no plasmid<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br>111.7 pSBLb4C15-red<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110 <br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow<br><br><h2>Todays work</h2><br><b>Streak from frozen stock:</b><br>60. PK401 dam- no plasmid<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>O/N:</b><br>60.3 PK401 dam- no plasmid*<br><i>*The O/N made on thursday used MRS, so it had to be remade from restreaks.</i><br><br><b>Plasmid preparation:</b><br>111.7 pSBLb4C15-red<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Restreak:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>no nr. pEL3S17-Thioredoxin<br><br><b>Amplification PCR</b><br>134. B0034-CBP<br>135. B0034-CSP<br>254. Gus<br>257. p-pem<br>258. pem<br><br><b>Mutagenesis</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>O/N Screening:</b><br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>221. pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>201. E.Coli. Nissle - CrtY-CrtI<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><b>Send to Linköping, as plasmid prepp and agar stabb</b><br>226.pSB1C3-pBAD/AraC-RBS-Luciferas (2012 years kit)<br>227.pSB1C3-pBAD/AraC-RBS-Luciferas (2013 years kit)<br><br><b>Transformation</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br>111.7 pSBLb4C15-red: 30,6 ng/µl<br><br><br><b>O/N:</b><br>221 failed, redo.<br><br><b>O/N Screening:</b><br>108.pEL3S17-J23106-B0034-IspA - Screening gel fail<br>109.pEL3S17-K206000-B0034-IspA - Screening gel fail<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis - <br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis - <br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His - Screening gel fail<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His - <br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 - Screening gel fail<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - Screening gel fail<br>219.pSB1C3-Miraculin - <br>221. pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>201. E.Coli. Nissle - CrtY-CrtI - Screening gel fail</div>'
+ }
+      else if(id == 'd2013810')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-10</h1><br><b>Name of participants: </b>Anders Edlund, Marcus H, Hampus.E, Kristoffer L, Ken B-A, Nils, Alexander, Sabri, Pontus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>134. B0034-CBP<br>135. B0034-CSP<br>254. Gus<br>257. p-Toxin-Antitoxin<br>258. Toxin-Antitoxin<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>259. pEL3S17-B0034-4CL-B0034-STS<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>259. pEL3S17-B0034-4CL-B0034-STS<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br><br><b>E. coli PK401 dam-:</b><br>60. no plasmid<br><br><h2>Todays work:</h2><br><b>Gel-electrophoresis</b><br>134. B0034-CBP*<br>135. B0034-CSP*<br>254. Gus<br>257. p-Toxin-Antitoxin<br>258. Toxin-Antitoxin<br>*PCR 99% failed<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>115. pSB3K5-J23101-B0034-His-TAL<br>114. pSB1C3-B0034-TAL(MUT)<br>120.pSB1C3-B0034-HIS-4CL<br><br><b>Amplification PCR</b><br>134. B0034-CBP<br>135. B0034-CSP<br><br><b>ON:</b><br>60. E. coli dam-<br><br><b>Assembly and subcloning:</b><br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>259. pEL3S17-B0034-4CL-B0034-STS<br>127 + 103 + 36 -> 253. pEL3S17-J23101-B0034-UGTCs2-His<br>152 + 61 + 111 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>152 + 62 + 111 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>95 + 58 + 36 -> 110.pEL3S17-K206001-B0034-IspA<br>CrtZ + 1 -> 184.pSB1C3-B0034-CrtZ<br>Miraculin + 1 -> 219.pSB1C3-Miraculin<br><br><b>O/N Screening:</b><br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>201. E.Coli. Nissle - CrtY-CrtI<br><br><b>DPN1</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>115 good, to sequencing<br>114 good, to sequencing<br>120 pick new clones and screen<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 - Succes<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - fail<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 -fail<br><br><b>Characterization experiments</b><br><b>Detection of TAL activity through spectroscopy (day 5)</b></div>'
+ }
+      else if(id == 'd2013811')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-11</h1><br><b>Name of participants: </b>Anders E, Mikael S, Nafisa B, Nils, Alexander, Sabri, Malin<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>60. PK401 dam- no plasmid<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110 <br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work:</h2><br><b>Competent cells:</b><br>60. PK401 dam- noplasmid<br><br><b>Transformation:</b><br>275. PK401 dam- pSBLb4C15-red (dam-)<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15 cut with E and P<br>and more.<br><br><b>Digest:</b><br>111.7 pSBLb4C15 cut with E and P<br>111.7 pSBLb4C15 cut with SacI and SalI<br>254. Gus P+X   <br>257. p-Toxin-Antitoxin P+X <br>258. Toxin-Antitoxin P+X<br>134. B0034-CBP E+S<br>1.  pSB1C3-red E+S<br>3. pSB3T5-red E+P<br><br><b>PCR purification:</b><br>254. pSB1C3-Gus<br>257. pSB1C3-p-Toxin-Antitoxin (with promotor)<br>258. pSB1C3-Toxin-Antitoxin<br>134. pSB1C3-B0034-CBP<br><br><b>PCR:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br><br><b>Plasmidprepp:</b><br>108.2.pEL3S17-J23106-B0034-IspA<br>108.3.pEL3S17-J23106-B0034-IspA<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><b>Two part assembly:</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br><br><b>O/N:</b><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br><br><b>Sekvensprepp:</b><br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Re-circularization:</b><br>202.pSB4C5.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 <br><br><b>Re-streak</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15 cut with E and P: correct bands!<br>and more.</div>'
+ }
+      else if(id == 'd2013812')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1><br><b>Name of participants: </b>Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>149. pSB1C3-B0034-Thioredoxin<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>114. pSB1C3-B0034-TAL-mut<br>115. pSB3K3-J23101-B0034-His-TAL<br>204. pEL3S17-J23110-B0034-HIS-STS<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work</h2><br><b>Ligation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-Gus<br>257. pSB1C3-p-Toxin-Antitoxin (with promotor)<br>258. pSB1C3-Toxin-Antitoxin<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Digest:</b><br>4.pSB1A3-red<br>6.pSB1K3<br>125. pSB1C3-ZCD-His<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>219.pSB1C3-Miraculin<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>275 PK401 dam- pSBLb4C15-red (dam-)<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><br><b>O/N Screening:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><b>Transformation</b><br>134. pSB1C3-CBP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Spreading:</b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>Plasmidprepp:</b><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Sequence prepp:</b><br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Transformation:</b><br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br><br><b>PCR:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><h2>Results:</h2><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His failed<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His failed<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br><br><h2>Characterization</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Mixing new buffers for western blot</b><br><br><b>Casting LB-agar plates:</b><br>Chloramphenicol: 55</div>'
+ }
+      else if(id == 'd2013812')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1><br><b>Name of participants: </b>Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>149. pSB1C3-B0034-Thioredoxin<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>114. pSB1C3-B0034-TAL-mut<br>115. pSB3K3-J23101-B0034-His-TAL<br>204. pEL3S17-J23110-B0034-HIS-STS<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work</h2><br><b>Ligation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-Gus<br>257. pSB1C3-p-Toxin-Antitoxin (with promotor)<br>258. pSB1C3-Toxin-Antitoxin<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Digest:</b><br>4.pSB1A3-red<br>6.pSB1K3<br>125. pSB1C3-ZCD-His<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>219.pSB1C3-Miraculin<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>275 PK401 dam- pSBLb4C15-red (dam-)<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><br><b>O/N Screening:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><b>Transformation</b><br>134. pSB1C3-CBP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Spreading:</b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>Plasmidprepp:</b><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Sequence prepp:</b><br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Transformation:</b><br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br><br><b>PCR:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><h2>Results:</h2><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His failed<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His failed<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br><br><h2>Characterization</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Mixing new buffers for western blot</b><br><br><b>Casting LB-agar plates:</b><br>Chloramphenicol: 55</div>'
+ }
+      else if(id == 'd2013813')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-13</h1><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Anton Berglund, Viktor Törnblom, Viktor Blomkvist, Christoffer Ahlström, Stephanie Herman, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L. Lovisa P, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>114.(2-3) pSB1C3-B0034-TAL-mut<br>115.(1-3) pSB3K3-J23101-B0034-His-TAL<br>204.(1-3) pEL3S17-J23110-B0034-HIS-STS<br><br><h2>Todays work:</h2><br><b>O/N Screening glycerolstock:</b><br>110.pEL3S17-K206001-B0034-IspA<br>219.pSB1C3-Miraculin<br>253.pEL3S17-J23101-B0034-UGTCs2-His<br><br><b>Assembly</b><br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><b>Ligation:</b><br>134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product])<br>134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product])<br>255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product])<br>255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product])<br>256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product])<br>256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product])<br><br><b>Plasmidprepp:</b><br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Sequencing prep:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>114.(2-3) pSB1C3-B0034-TAL-mut<br>115.(1-3) pSB3K3-J23101-B0034-His-TAL<br>204.(1-3) pEL3S17-J23110-B0034-HIS-STS<br>108.2.pEL3S17-J23106-B0034-IspA<br>108.3.pEL3S17-J23106-B0034-IspA<br>163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Re-streaking:</b><br>134. pSB1C3-CBP (CBP1 [134.1] from digested plasmids [PCR-product])<br>134. pSB1C3-CBP (CBP2 [134.2] from digested plasmids [PCR-product])<br>254. pSB1C3-gus (gus1 [254.1] from digested plasmids [PCR-product])<br>254. pSB1C3-gus (gus2 [254.2] from digested plasmids [PCR-product])<br>254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product])<br>255. pSBLb4C15-pemp (pemp1 [257.1] from digested plasmids [PCR-product])<br>255. pSBLb4C15-pemp (pemp2 [257.2] from digested plasmids [PCR-product])<br>256. pSBLb4C15-pem (pem1 [258.1] from digested plasmids [PCR-product])<br>256. pSBLb4C15-pem (pem2 [258.2] from digested plasmids [PCR-product])<br>256. pSBLb4C15-pem (pem3 [258.3] from digested plasmids [PCR-product])<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><b>Re streak 14/8 or 15/8</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br><br><b>Transformation & plating</b><br>134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product])<br>134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product])<br>255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product])<br>255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product])256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product])<br>256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product])<br>225. pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>261. pSB3K3-J23110-B0034-HIS-4CL<br>120. pSB3K3-B0034-HIS-4CL<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>177pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br><br><b>Re-streaking</b><br>254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product]): 1 clone<br>255. pSBLb4C15-pemp (pemp3 [257.3] from digested plasmids [PCR-product]): 4 clones<br><br><b>O/N:</b><br>275. pSBLb4C15-red (dam-)<br>276. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam)<br>277. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam)<br><br><b>Gradient PCR:</b> <br>CBP (colony PCR from Lb.Reuteri)<br>CSP (colony PCR from Lb.Reuteri)<br>Mcherry (from plasmid preparation)<br>256. pSBLb4C15-pem<br><br><h2>Results</h2><br>One of the two gels from SDS page was succesfull, still have problems with the buffers<br><br><h2>Characterization</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><br><b>Western blot</b><br><b>SDS-page on constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><br><b>Electro transfer over night</b><br><br><h2>Other experiments</h2><br><b>Casting LB-agar plates:</b><br>Chloramphenicol: 85<br></div>'
+ }
+      else if(id == 'd2013814')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-14</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Mikael S, Viktor B, Viktor T, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Peter C, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work:</h2><br><b>Re-streak:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>pSB1K3-J23110-B0034-aeBlue (should be subcloned to pSB1C3 after)<br>pSB3K3-J23110-B0034-AmilCP (should be subcloned to pSB1C3 after)<br>pSB3K3-J23110-B0034-asCP (should be subcloned to pSB1C3 after)<br>Re streak 15/8 or 16/8<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>O/N:</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>Streak from frozen stock:</b><br>111. pSBLb4C15-red<br><br><b>Gradient touchdown colony PCR:</b><br>CSP <br><br><b>Gel Electrophoresis:</b><br>CBP (colony PCR from Lb.Reuteri)<br>CSP (colony PCR from Lb.Reuteri)<br>Mcherry (from plasmid preparation)<br>256. pSBLb4C15-pem<br><br><b>PCR purification: </b><br>M1 <br>M2<br>M3<br>M4<br>CB1<br>CB2<br>256. pSBLb4C15-pem (P1)<br><br><b>Digestion:</b><br>M3 (x+p)<br>CB1 (x+p)<br>P1 (x+p)<br>111.7 pSB4C15-red (e+p)<br>Bleomycin resistance cassette (SacI)<br>pEL PCR product with e. coli ori (ClaI)<br>Lb3 reuteri DSM 20016 pVS2 (ClaI)<br>CP promoters with amilGFP, aeBlue,tsPurple<br><br><b>Ligation:</b><br>M3 with pSB1C3, pSBLB4C15 + CP8, CP1<br>CB1 with pSB1C3<br>P1 with pSB1C3<br>pVS2 origin of replication with pEL PCR-product, cut by ClaI <br>CP promoters with amilGFP, aeBlue,tsPurple<br><br><b>Frozen stock and plasmid preparation:</b><br>275.1 pSBLb4C15-red (dam-)*<br>275.2 pSBLb4C15-red (dam-)<br>276. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><i>*O/N of clone 1 was made from a very small colony, its colour wasn’t as strong as clone 2’s after growing overnight.</i><br><br><b>Transformation:</b><br>109.pEL3S17-K206000-B0034-IspA x<br>110.pEL3S17-K206001-B0034-IspA x<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His x<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His x<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His x<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His x<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His x<br>184.pSB1C3-B0034-CrtZ x<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His x<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His x<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His x<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His x<br>267.psB3K3-K206000-B0034-IspA x<br>268.psB3K3-K206001-B0034-IspA x<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His x<br>272 pSB1K3-B0034-Thioredoxin-ZCD-His x<br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x<br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x<br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His x<br><br><b>Plasmid prepp:</b><br>280. pSB1A3-B0034-aeBlue (should be subcloned to pSB1C3 after, give Erik the subclones version)<br><br><h2>Results:</h2><br><b>Gel Electrophoresis:</b><br>CBP (colony PCR from Lb.Reuteri)<br>CB1: 54.3o C (NC ok!), CB2: 63.3o C (NC ok!) → Correct bands!!<br>CSP (colony PCR from Lb.Reuteri) <br>CS1: 55.7o C (NC ok!), CS2: 65.6o C (NC ok!) → No bands!! (not even primer dimer) <br>Mcherry (from plasmid preparation)<br>M1: 45.0o C (NC ok!), M2: 47.9o C (NC ok!), M3: 50.5o C (NC ok!), M4: 54.3o C (NC ok!) → Correct bands!!<br>256. pSBLb4C15-pem: <br>P1: 47.9o C (NC ok!) → Correct bands!!<br><br><b>PCR purification: </b><br>M1: 65.9 ng/µl → Store in freezer!<br>M2: 200.6 ng/µl → Store in freezer!<br>M3: 136.8 ng/µl → For digest! → Store in freezer!<br>M4: 49.5 ng/µl → Store in freezer!<br>CB1: 90.8 ng/µl → For digest! → Store in freezer!<br>CB2: 56.7 ng/µl → Store in freezer!<br>P1: 56.2 ng/µl → For digest! → Store in freezer!<br><br><b>Plasmid preparation:</b><br>275.1 PK401 dam- pSBLb4C15-red (dam-):				13.5 ng/µl<br>275.2 PK401 dam- pSBLb4C15-red (dam-):				27.9 ng/µl<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-): 18.3 ng/µl<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-): 26.6 ng/µl<br><br><b>Western blot:</b><br>Transfer overnight not complete but continued with anyway<br>Very promising results, bands can be seen around 50 kDa but the ladder is pretty weak so we can’t be absolutely sure. Looks like the J23101 promotor does’nt work.<br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><b>Blotting and development of constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><h2>Other experiments:</h2><br><b>Making MRS-broth (2x600 ml)</b><br><br><b>New buffers for western blot made </b></div>'
+ }
+      else if(id == 'd2013815')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-15</h1><br><b>Name of participants: </b> Christoffer Ahlström, Stephanie Herman, Alona Nyberg, Mikael Strandgren, Alexander, Malin, Pontus, Niclas, Christoffer, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H., Peter C, Lovisa P, Theodor L<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Digestions:</b><br>Erythromycin resistance cassette from pLUL631 (SacI, Tango buffer)<br>Bleomycin resistance cassette (SalI, FD buffer)<br><br><b>O/N Screening glycerolstock:</b><br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>184.pSB1C3-B0034-CrtZ<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>110.pEL3S17-K206001-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Plasmid prep:</b><br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br><br><b>Transformation:</b><br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>292. PK401 dam- pSBLb4C15-CP1-mCherry (dam-)<br>294. PK401 dam- pSBLb4C15-CP8-mCherry (dam-)<br><br><b>Ligations:</b><br>pSBLB4C15 and Bleomycin resistance cassette cut with SacI and SalI<br><br><b>Spreading of strains:</b><br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br><i>E. coli PK401 dam-:</i><br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><b>Screening PCR and gel electrophoresis</b><br><br><h2Results:</h2><br><b>Yesterdays buffers for western blot resulted in smear. </b><br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>47 Chloramphenicol<br>47 Without antibiotics<br><br><b>Testing yesterdays buffers for western blot.</b><br> <br><b>New separation- and stacking buffers for western blot with a factor 1000 concentration of Tris.</b></div>'
+ }
+      else if(id == 'd2013816')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-16</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Mikael Strandgren, Alexander, Pontus, Nils, Christoffer, Sabri, Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L, Peter C<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>O/N Screening & glycerolstock:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>184.pSB1C3-B0034-CrtZ<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>2 74.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br><br><b>Digestion</b><br>Erythromycin cassette amplicon from pLUL631 (SalI)<br><br><b>Ligation</b><br>pSB1C3-pemp<br>298. pSBLb4E15-red (E-cassette amplicon from pLUL631 with pSBLb4C15 (SacI + SalI))<br>297. pSBLb4B15-red (Bleomycin cassette with pSBLb4C15 (SacI + SalI))<br><br><b>Plasmid preparation:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>204.4 pEL3S17-J23110-B0034-HIS-STS<br>254.1 pSB1C3-gus<br>254.2 pSB1C3-gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>255.3 pSBLB4C15-pemp<br>255.4 pSBLB4C15-pemp<br><br><b>O/N:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>204.4 pEL3S17-J23110-B0034-HIS-STS<br>254.1 pSB1C3-gus<br>254.2 pSB1C3-gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>255.3 pSBLB4C15-pemp<br>255.4 pSBLB4C15-pemp<br>257.1 pSB1C3-pemp<br>257.2 pSB1C3-pemp<br>257.3 pSB1C3-pemp<br>280. pSB1K3-J23110-B0034-aeBlue<br>281. pSB3K3-J23110-B0034-amilCP<br>282. pSB3K3-J23110-B0034-asCP<br><br><b>Transformation:</b><br>297. PK401 dam- pSBLb4B15-red <br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>155pSB1C3-j23110-RBS-meffRed<br>156pSB1C3-j23106-RBS-meffRed<br>157pSB1C3-j23110-RBS-spisPink<br>158pSB1C3-j23106-RBS-spisPink<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>247pSB1C3-J23110-RBS-tsPurple<br>248pSB1C3-J23106-RBS-tsPurple<br>249pSB1C3-J23110-RBS-scOrange<br>250pSB1C3-J23106-RBS-scOrange<br>251pSB1C3-J23110-RBS-fwYellow<br>252pSB1C3-J23106-RBS-fwYellow<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><h2>Results:</h2><br><b>Plasmid prep:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS: 9.7 ng/µl<br>204.3 pEL3S17-J23110-B0034-HIS-STS: 11.9 ng/µl<br>204.4 pEL3S17-J23110-B0034-HIS-STS: 7.6 ng/µl<br>254.1 pSB1C3-gus: 6.7 ng/µl<br>254.2 pSB1C3-gus: 11.5 ng/µl<br>255.1 pSBLB4C15-pemp: 9.9 ng/µl<br>255.2 pSBLB4C15-pemp: 6.3 ng/µl<br>255.3 pSBLB4C15-pemp: 5.0 ng/µl<br>255.4 pSBLB4C15-pemp: 4.6 ng/µl<br><i>Since the concentrations were so low a new set of overnight cultures were prepared.</i><br><br><b>Spectophotometry:</b><br>Inconclusive results.<br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><b>Testing of new buffers for western blot.</b><br><br><h2>Other experiments</h2><br><b>Making MRS-broth (2x600 ml)</b><br><br><b>Making LB-agar plates:</b><br>173 Chloramphenicol <i>(chloramphenicol was forgotten in 26 of the plates, but they had already been mixed with 25 others, so also these had to be discarded. 5 plates of doubtful antibiotic content had already been used by the carotenoid group, they were probably ones with ab. E. colis containing pSB3K3 were spread on some of the mixed plates.)</i><br>28 Kanamycin</div>'
+ }
+      else if(id == 'd2013817')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-17</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Sabri, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Restreak</b><br>254.3 pSB1C3-Gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>297. pSBLb4B15-red <br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>Plasmid preparation:</b><br>254.1 pSB1C3-Gus<br>254.2 pSB1C3-Gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>257.1 pSB1C3-pemp<br>257.2 pSB1C3-pemp<br>184.pSB1C3-B0034-CrtZ<br>280. pSB1K3-J23110-B0034-aeBlue<br>281. pSB3K3-J23110-B0034-amilCP<br>282. pSB3K3-J23110-B0034-asCP<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin (X,P)<br><br><b>Ligation:</b><br>147.pSB1C3-B0034-Thioredoxin, 91.BBa_J61002 (amp)-J23106, 36.pEL3S17-red<br>---> 300.pEL3S17-J23106-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin, 92.BBa_J61002 (amp)-J23110, 36.pEL3S17- red<br>---> 301.pEL3S17-J23110-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin, 103.pSB1K3-J23101, 36.pEL3S17-red<br>---> 299.pEL3S17-J23101-B0034-Thioredoxin<br><br><b>Transformation:</b><br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Plasmid preparation:</b><br>254.1 pSB1C3-Gus 110 ng/uL<br>254.2 pSB1C3-Gus 132 ng/uL<br>255.1 pSBLB4C15-pemp156 ng/uL<br>255.2 pSBLB4C15-pemp 158 ng/uL<br>257.1 pSB1C3-pemp 146 ng/uL<br>257.2 pSB1C3-pemp 140 ng/uL</div>'
+ }
+      else if(id == 'd2013818')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H.<br><br><h2>Ongoing constructs:</h2><br><h2>E. coli:</h2><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>ON:</b><br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>pSBLB4C15-red<br>pSBLB4C15-red dam-<br><br><b>Restreak:</b><br>68.1 pSB3K3-B0034-BFP<br>68.2 pSB3K3-B0034-BFP<br>68.4 pSB3K3-B0034-BFP<br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.pSB1C3-B0034-Thioredoxin<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.pSB3K3-K206000-B0034-IspA <br>268.pSB3K3-K206001-B0034-IspA <br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.pEL3S17-J23106-B0034-Thioredoxin<br><br><b>PCR:</b> <br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Digest:</b><br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Ligation:</b><br>4.pSB1A3-red<br>7.pSB3K3-red<br>36.pEL3S17-red<br>91.j23106<br>92.j23110<br>103.pSB1K3-j23101<br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Plasmidprep:</b><br>184.pSB1C3-B0034-CrtZ<br><br><b>Transformation:</b><br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>302. idi (mut)-B0034-Ispa<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>305.pSB1A3-j23110-B0034-CrtZ<br><br><b>O/N screening:</b><br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><h2>Results:</h2><br><b>Failed:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'
+ }
+      else if(id == 'd2013819')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1><br><b>Name of participants: </b>Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Screening</b><br>134.1 pSB1C3-CBP<br>254 pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI<br>254.2 pSB1C3-gus: XbaI & PstI<br>255.1 pSBLB4C15-pemp: EcoRI & PstI<br>255.2 pSBLB4C15-pemp: EcoRI & PstI<br>257.1 pSB1C3-pemp: EcoRI & PstI<br>257.2 pSB1C3-pemp: EcoRI & PstI<br><br><b>Frozen stock:</b><br>134.1 pSB1C3-CBP<br>275. pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>1 ml overnight culture:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red<br>111.7(2) pSBLB4C15-red<br>134.1 pSB1C3-CBP<br>275 pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Overnight culture:</b><br>Lb11.2 plantarum 36E-no plasmid<br><br><b>Phusion PCR:</b><br>usp45 (synthesized signal peptide from Lactococcus lactis)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>digest</b><br>36. pEL3S17-red<br><br><b>Ligation</b><br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results:</h2><br><b>Screening</b><br>134.1 pSB1C3-CBP: showed the correct band!<br>254 pSB1C3-gus: showed a very weak band, not sure if correct!<br>288.1 pSB1C3-pem: showed the correct band!<br>288.2 pSB1C3-pem: showed the correct band!<br>288.3 pSB1C3-pem: did not show the correct band!<br>288.4 pSB1C3-pem: showed the correct band!<br>289.1 pSB1C3-mCherry: showed the correct band!<br>289.2 pSB1C3-mCherry: did not show the correct band!<br>289.3 pSB1C3-mCherry: showed the correct band!<br>289.4 pSB1C3-mCherry: showed the correct band.<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br>257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red: 81.7 ng/µl<br>111.7(2) pSBLB4C15-red: 93.9 ng/µl<br>134.1 pSB1C3-CBP: 163.8 ng/µl<br>275 pSBLB4C15-red (dam-): 92.4 ng/µl<br>288.1 pSB1C3-pem: 211.2 ng/µl<br>288.2 pSB1C3-pem: 193.2 ng/µl<br>288.4 pSB1C3-pem: 193.9 ng/µl<br>289.1 pSB1C3-mCherry: 230.4 ng/µl<br>289.3 pSB1C3-mCherry: 229.7 ng/µl<br>289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'
+ }
+      else if(id == 'd2013820')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Characterisation of the CP promoters with facs:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Digestion:</b><br>USP. with E, P<br>191. pSB1A2-fusionGFP with X, P<br>280. pSB1A3-B0034-aeBlue with X, P <br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><b>Making competent Lactobacillus reuteri</b><br>Lb12.1 reuteri DSM 20016 plasmid free: eight<br>Lb12.2 reuteri DSM 20016 plasmid free: eight<br><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: two<br>111.7(1) pSBLB4C15-red: one<br>111.7(2) pSBLB4C15-red: two<br>255.1 pSBLB4C15-pemp: one<br>255.2 pSBLB4C15-pemp: one<br>275 pSBLB4C15-red (dam-): three<br><br><h2>Results:</h2><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: one of the two survived<br>111.7(1) pSBLB4C15-red: survived<br>111.7(2) pSBLB4C15-red: one of the two survived<br>255.1 pSBLB4C15-pemp: survived<br>255.2 pSBLB4C15-pemp: survived <br>275 pSBLB4C15-red (dam-): two of the three survived</div>'
+ }
+      else if(id == 'd2013821')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1><br><b>Name of participants: </b>Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>204(1-4). pEL3S17-J23110-B0034-His-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Digestion:</b><br>usp45: XbaI & AgeI<br>191.1 pSB1A2-fusionGFP: XbaI, PstI<br>191.1 pSB1A2-fusionGFP: NgoMIV & PstI<br>275. pSBLB4C15-red (dam-): EcoRI & PstI<br>289.1 pSB1C3-mCherry: NgoMIV & PstI<br>289.1 pSB1C3-mCherry: XbaI, PstI<br>297.1 pSBLB4B15-red (dam-): EcoRI & PstI<br><br><b>Ligation:</b><br>pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1)<br>pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1)   		 <br>pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1)   <br>pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1)    <br>pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1)   	 <br>pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1)   	 <br>pSB1C3-usp45 (1+usp45)<br>pSB1C3-usp45 (6+usp45)<br>pSBLb4C15-CP1-B0034-BFP<br>pSBLb4C15-CP8-B0034-BFP<br>pSBLb4C15-CP11-B0034-BFP<br>pSBLb4C15-CP29-B0034-BFP<br>pSBLb4C15-CP30-B0034-BFP<br>pSBLb4C15-CP41-B0034-BFP<br>pSBLb4C15-CP44-B0034-BFP<br>pSBLb4C15-CP11-aeblue<br>pSBLb4C15-CP30-aeblue<br>pSBLb4B15-CP11-mcherry<br>pSBLb4B15-CP30-mcherry<br>pSBLb4B15-CP11-tspurple<br>pSBLb4B15-CP30-tspurple<br>pSBLb4B15-CP11-amilCP<br>pSBLb4B15-CP30-amilCP<br>pSBLb4B15-CP11-amilGFP<br>pSBLb4B15-CP30-amilGFP<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Transformation:</b><br>108.pEL3S17-J23106-B0034-IspA<br>141.pRL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>204(1-4)<br> 	<br><b>Re-streak:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Screen/ ON:</b><br>219.pSB1C3-Miraculin<br>304.pSB1A3-J23106-B0034-CrtZ<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>280. pSB1A3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP <br><br><b>Plasmid prep. </b><br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br><br><b>Mutagenesis PCR:</b><br>336. pSB1C3-spisPink<br>337. pSB1C3-scOrange<br>338. pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (20µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R<br><br><b>Cleaning of electroporation cuvettes</b></div>'
+ }
+      else if(id == 'd2013822')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1><br><b>Name of participants: </b>Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Competent cell preparation:</b><br>Lb11.1 plantarum 36E no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br>Lb12.2 reuteri DSM 20016 no plasmid<br><br><b>Digest:</b><br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><strike>3.2. pVS2 (died in electroporation)<br>310. pSBLB4B15-CP11-fusionGFP (died in electroporation)<br>318. pSBLB4B15-CP11-mCherry (died in electroporation)<br>320. pSBLB4B15-CP11-amilGFP (died in electroporation)<br>313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation)<br>317. pSBLB4B15-CP30-tsPurple (died in electroporation)<br>331. pSBLB4C15-CP41-BFP (died in electroporation)<br>332. pSBLB4C15-CP44-BFP (died in electroporation)</strike><br><b>+ other E. coli transformations</b><br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><h2>Results:</h2><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do.<br>288. pSB1C3-pem:  				--”--<br><br><b>Transformation:</b><br>11.1 Lb. plantarum 36E:<br>3.2 pVS2: survived electroporation<br>3.2 pVS2: died during electroporation<br>310. pSBLB4B15-CP11-fusionGFP: died during electroporation<br>311. pSBLB4B15-CP30-fusionGFP: survived electroporation<br>312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation<br>313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation<br>316. pSBLB4B15-CP11-tsPurple: survived electroporation<br>317. pSBLB4B15-CP30-tsPurple: died during electroporation<br><br><b>12.2 Lb. reuteri DSM 20016:</b><br>3.2 pVS2: survived electroporation<br>318. pSBLB4B15-CP11-mCherry: died during electroporation<br>319. pSBLB4B15-CP30-mCherry: survived electroporation<br>320. pSBLB4B15-CP11-amilGFP: died during electroporation<br>321. pSBLB4B15-CP30-amilGFP: survived electroporation<br>322. pSBLB4B15-CP11-amilCP: survived electroporation<br>323. pSBLB4B15-CP30-amilCP: survived electroporation<br>324. pSBLB4C15-CP11-aeBlue: survived electroporation<br><br><b>12.1 Lb. reuteri DSM 20016:</b><br>325. pSBLB4C15-CP30-aeBlue: survived electroporation<br>326. pSBLB4C15-CP1-BFP: survived electroporation<br>327. pSBLB4C15-CP8-BFP: survived electroporation<br>328. pSBLB4C15-CP11-BFP: survived electroporation<br>329. pSBLB4C15-CP29-BFP: survived electroporation<br>330. pSBLB4C15-CP30-BFP: survived electroporation<br>331. pSBLB4C15-CP41-BFP: died during electroporation<br>332. pSBLB4C15-CP44-BFP: died during electroporation<br><br><h2>Characterization:</h2><br><b>Western blot analysis (SDS-page) on constructs:</b><br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15) (neg.contr)<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (60 µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R</div>'
+ }
+      else if(id == 'd2013823')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>E. coli (D5α):</b><br>111.7 pSBLb4C15-red<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites)<br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>Touchdown with Tm1+2 and Tm1+4.<br><br><b>Transformation:</b><br>102.pSB1C3-CrtE<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>Re-streak:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Screening O/N:</b><br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>O/N:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Glycerol stock Plasmidprep/sekvensprep:</b><br>108.pEL3S17-J23106-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>299.pEL3S17-J23101-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>111.7 pSBLb4C15-red (cut with E and P): No band<br><i>	*→ redo mutagenesis PCR with higher temperatures</i></div>'
+ }
+      else if(id == 'd2013824')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-24</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>Escherichia coli</b><br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br>136. pSB1C3-B0034-USP<br>pSB1C3-B0034-usp45<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><b>Lactobacillus reuteri:</b><br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><br><b>Lactobacillus plantarum:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue <br><br><b>Digest:</b><br>200.pSB1C3-B0034-asCP E,X<br>148.pSB1C3-RBS-gfasPurple E,X<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br><br><b>Ligation:</b><br>200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→<br>→ 343.J23106-B0034-asCP<br>148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→<br>→ 160.j23106-RBS-gfasPurple<br>148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→<br>→ 159j23110-RBS-gfasPurple<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→<br>→215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Transformation:</b><br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>343.pSB1C3-J23106-B0034-asCP<br>160.pSB1C3-j23106-RBS-gfasPurple<br>159.pSB1C3-j23110-RBS-gfasPurple<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>336.pSB1C3.spisPink<br>337.pSB1C3.scOrange<br>284.pSB1C3.B0034-aeBlue<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>284. pSB1C3-B0034-aeBlue<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>O/N screening:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>136.<br>312<br>313<br>315<br>321<br>325<br>327<br>332<br>317<br><br><b>O/N:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Plasmid prepp & sequence prepp</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Gel electrophoresis:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><i>+ E. coli transformations</i><br><br><b>PCR-purification:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>DpnI:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><h2>Results:</h2><br><b>Transformations:</b><br>All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. <br>Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. <br><br><b>Gel electrophoresis:</b><br>All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. <br>The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite.<br>The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'
+ }
+      else if(id == 'd2013825')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1><br><b>Name of participants:</b> Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin<br><br><b>Ligate:</b><br>147.pSB1C3-B0034-Thioredoxin+CP1→<br>→ 346.pSB1C3-CP1-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP8→<br>→347.pSB1C3-CP8-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP41→<br>→348.pSB1C3-CP41-B0034-Thioredoxin<br><br><b>Transformation:</b><br>1.pSB1C3-Red (competens test)<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ<br>336.pSB1C3-spisPink<br>337pSB1C3-scOrange<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348.pSB1C3-CP41-B0034-Thioredoxin<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br><br><b>Re-streak:</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Sequenceprep:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br></div>'
+ }
+      else if(id == 'd2013826')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1><br><b>Name of participants: </b>Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><h2>Todays work</h2><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br>Slovenian 4CL with His-primers<br><br><b>Gel electrophoresis:</b><br>Erythromycin-cassette PCR-product from Lb2.1 pLUL631<br>Erythromycin-cassette PCR-product from Lb2.2 pLUL631<br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br><br><b>Assembly & transformation:</b><br>349<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Re streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>Plasmidprepp:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>O/N screen</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Screening:</b><br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348. pSB1C3-CP41-B0034-Thioredoxin<br><br><b>O/N:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades)<br><br><b>Re-streak</b><br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades)<br><br><b>Sekvensprep:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Ligation:</b><br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → <br>→215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Results:</h2><br><b>Gel electrophoresis: </b><br>Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly<br>Erythromycin-cassette PCR-product:  			--”--<br>Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed<br>77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp.<br>269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made.<br><br><h2>Characterization:</h2><br><b>Western blot sample preparation:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparing MRS-agar</b><br><br><b>Preparing low salt LB-agar</b></div>'
+ }
+      else if(id == 'd2013827')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday  2013-08-27</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>O/N:</b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br><br><b>Transformation:</b><br>Chromoproteins into pSB3K3<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1K3-CP1-B0034-Thioredoxin<br>347.pSB1K3-CP8-B0034-Thioredoxin<br>348.pSB1K3-CP41-B0034-Thioredoxin<br><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br><br><b>Screening PCR: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br><br><b>Sekvensprep:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>284. pSB1C3-B0034-aeBlue<br><br><b>Re-streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>PCR:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures Tm1 and +5°C (with touchdown))</i><br><br><b>Streaking of plasmid free stock on test plates:</b><br><i>- e. coli (10. and 60.3) onto erythromycin LB-plates:</i><br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><i>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:</i><br>50 µg/ml<br>100 µg/ml<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp<br>77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp<br><br><h2>Other experiments</h2><br><b>Preparing test plates, to determine minimum antibiotic concentration:</b><br>Lb-erythromycin:<br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><br><b>MRS-zeocin:</b><br>50 µg/ml<br>100 µg/ml</div>'
+ }
+      else if(id == 'd2013828')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday  2013-08-28</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><h2>Todays work</h2><br><b>Gel electrophoresis: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>77. pSB4C15-red (annealing at Tm1): <br>77. pSB4C15-red (annealing at Tm1+5): <br><br><b>Ligation: </b><br>352. pSBLbC3-red (pEL4C15 (ClaI) + pVS2 (ClaI))<br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>PCR: </b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R (Tm1)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><br><b>Digest</b><br>36. pEL3S17-red<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results</h2><br><b>Streaking of plasmid free stock on test plates:</b><br>- e. coli (10. and 60.3) onto erythromycin LB-plates:<br>5 µg/ml: Growth <br>10 µg/ml: Growth<br>20 µg/ml: Growth<br>50 µg/ml: Still some growth, but less → do new plates with higher conc. and try 50 µg/ml-plates from Erik Gullberg in case our antibiotic has lost efficiency.<br>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:<br>50 µg/ml: Growth<br>100 µg/ml: Growth (Plantarum grew less, but did so also on antibiotic free plates.)<br>- lactobacillus (Lb4., Lb11. and Lb12.) onto antibiotic-free MRS-plates:<br>Everything grew on Ab-, plantarum slightly less.<br><br><b>Gel electrophoresis: </b><br>351.1 pSB1C3-pemp-mut1: A weak band at ~1000 bp<br>351.2 pSB1C3-pemp-mut1: Nothing<br>77. pSB4C15-red (annealing at Tm1): Nothing → do a new PCR at TM1.<br>77. pSB4C15-red (annealing at Tm1+5): Nothing. <br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: Failed<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: Failed<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: Failed<br><br><b>Re-streak:</b><br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Other experiments</h2><br><b>Preparation of new test-plates:</b><br>LB-erythromycin:<br>100 µg/ml<br>200 µg/ml</div>'
+ }
+      else if(id == 'd2013829')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday  2013-08-29</h1><br><b>Name of participants: </b>Mikael Strandgren, Viktor Blomkvist, Alona Nyberg, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>353. pSBLb4E15<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>353. pSBLb4E15<br><br><b>Plasmidprep:</b><br>223<br>346<br><br><h2>Results</h2><br><b>Streaking of plasmid free stock on test plates:</b><br>- e. coli (10. and 60.3) onto erythromycin LB-plates:<br>100 µg/ml: Weak growth<br>200 µg/ml: Possibly no growth, let them “grow” some more. <br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparation of new test-plates:</b><br>MRS-Zeocin:<br>250 µg/ml<br></div>'
+ }
+      else if(id == 'd2013830')
+ {
+	ds = '<div id="dairy-text"><h1>Friday  2013-08-30</h1><br><b>Name of participants: </b>Mikael Strandgren, Viktor Björklund, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>77. pSB4C15-red <br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>Lb9. faecalis JH2-2 pAMbeta<br><br><h2>Todays work</h2><br><b>Gel extraction:</b><br>77. pSB4C15-red (annealing at Tm1):  <br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R*<br>*Since the product from monday disappeared.<br><br><b>PCR purification:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori<br><br><b>Digestion:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori (PCR product)(XbaI and BglII)<br>77. pSB4C15-red (PCR product) (NheI and BglII) <br><br><b>Ligation:</b><br>169. pSBLbEcC2-red (shuttle-vector with pAMbeta and e. coli replicon)<br><br><h2>Results</h2><br><b>Western blot:</b> <br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd2013831')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday  2013-08-31</h1><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br>386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1)<br><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation)<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'
+ }
+      else if(id == 'd201391')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-01</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red<br>406. pSBLbC3-red (dam-)<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br>406. pSBLbC3-red (dam-)<br><br><b>Screening:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2)<br><br><b>Re-streak:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br><br><h2>Results</h2><br>First screening failed (weird gel)<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201392')
+ {
+	ds = '<div id="dairy-text"><h1>Monday  2013-09-02</h1><br><br><b>Name of participants:</b> Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br><b>O/N:</b><br>169. pSBLbEcC2-red<br><br><b>Mutagenesis PCR:</b><br>111.7 pSBLb4C15-red *<br>*with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night.<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br>352. pSBLbC3-red*<br>*New attempts at ligation, just in case.<br><br><h2>Results: </h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: No bands<br><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201393')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br>Frozen stock and plasmid preparation:<br>169. pSBLbEcC2-red<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red* <br><i>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</i><br><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br><i>*New attempts at ligation, just in case.</i><br><br><b>Sequencing preparation:</b><br>169. pSBLbEcC2-red<br><br><h2>Characterization experiments:</h2><br><b>Western blot sample preparation on construct:</b><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Results: </h2><br><b>Frozen stock and plasmid preparation:</b><br>169. pSBLbEcC2-red: 44.2 ng/µl<br>169. pSBLbEcC2-red: 47.8 ng/µl<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'
+ }
+      else if(id == 'd201394')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>Assembly:</b><br>353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old)<br><br><b>Trasformation:</b><br>111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP)<br>353. pSBLb4E15-red <br><br><b>PCR-purification:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)<br><br><b>DpnI-treatment:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'
+ }
+      else if(id == 'd201395')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1><br><b>Name of participants:</b> Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs:</h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work</h2><b>First day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201396')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>PCR-purification and recircularization:</b><br>Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30)<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red*<br><i>*to do a new plasmid preparation since old may have corrupt red-cassette/contamination)</i><br><br><b>Streak from frozen stock:</b><br>111.7 pSBLb4C15-red*<br><i>* to test if red-cassette has been damaged, or possibly the stock contaminated.</i><br><br><b>Second day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201397')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1><br><br><b>Name of participants:</b> Mikael Strandgren, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work:</h2> <br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE) <br>355.2 pSB3K3-CP29-mCherry<br>355.3 pSB3K3-CP29-mCherry<br>357.3 pSB3K3-CP30-usp45-mCherry<br>357.4 pSB3K3-CP30-usp45-mCherry<br>358.1 pSB3K3-CP29-usp45-mCherry<br>358.2 pSB3K3-CP29-usp45-mCherry<br>363. pSB3K3-CP30-tspurple<br>364. pSB3K3-CP29-tspurple<br>365. pSB3K3-CP11-tspurple<br>366. pSB3K3-CP30-amilCP<br>367. pSB3K3-CP29-amilCP<br>368. pSB3K3- CP11-amilCP<br>369. pSB3K3-CP30-aeblue<br>370. pSB3K3-CP29-aeblue<br>371. pSB3K3-CP11-aeblue<br><br><b>Assembly:</b><br>407. pSB1C3-CP29-Mcherry*<br>408. pSB1C3-CP30-usp45-mCherry*<br>409. pSB1C3-CP29-usp45-mCherry*<br>410. pSB1C3-CP30-tsPurple*<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP* <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue*<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old)<br>* pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today.<br><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>417. pSB1C3-CP29-aeBlue <br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red <br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette.<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red<br><br><b>Third day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Results: </h2><br><b>O/N:</b><br>111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today.<br><br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl<br>355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl<br>355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl<br>357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl<br>357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl<br>358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl<br>358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl<br>363. pSB3K3-CP30-tspurple: 75.5 ng/µl<br>364. pSB3K3-CP29-tspurple: 43.9 ng/µl<br>365. pSB3K3-CP11-tspurple: 47.3 ng/µl<br>366. pSB3K3-CP30-amilCP: 74.5 ng/µl<br>367. pSB3K3-CP29-amilCP: 77.2 ng/µl<br>368. pSB3K3- CP11-amilCP: 34.3 ng/µl<br>369. pSB3K3-CP30-aeblue: 37.4 ng/µl<br>370. pSB3K3-CP29-aeblue: 60.3 ng/µl<br>371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'
+ }
+      else if(id == 'd201398')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-08</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work: </h2><br><b>Transformations:</b><br>411. pSB1C3-CP29-tsPurple *<br>412. pSB1C3-CP11-tsPurple *<br>414. pSB1C3-CP29-amilCP *<br>415. pSB1C3-CP11-amilCP *<br>417. pSB1C3-CP29-aeBlue *<br>418. pSB1C3-CP11-aeBlue *<br>* The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good.<br><br><b>ON:</b><br>353. pSBLB4E15-red<br><br><h2>Results: </h2><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today.<br>412. pSB1C3-CP11-tsPurple: 			“ <br>414. pSB1C3-CP29-amilCP 			“<br>415. pSB1C3-CP11-amilCP 			“<br>417. pSB1C3-CP29-aeBlue 			“<br>418. pSB1C3-CP11-aeBlue			“<br>419. pSBLbEcE-red: Growth covering the plates, only white colonies.<br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'
+ }
+      else if(id == 'd201399')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1><br><b>Name of participants: </b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Digest</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br> <br><b>Ligation</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Fourth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br></div>'
+ }
+      else if(id == 'd2013910')
+ {
+	ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1><br><b>Name of participants:</b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Re-streak</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br><br><b>Fifth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br></div>'
+ }
+      else if(id == 'd2013911')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1><br><b>Name of participants: </b>Sabri<br><br><h2>Todays Work:</h2><br><b>O/N:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+      else if(id == 'd2013912')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1><br><b>Name of participants:</b> Sabri<br><br><h2>Todays Work:</h2><br><b>Plasmid prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br> <br><b>Sequence prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+ else if(id == 'd20131013')
+ {
+  ds ='<h1>European Jamboree Lyon end</h1>';
+ }
+ else if(id == 'd20131012')
+ {
+  ds ='<h1>European Jamboree Lyon</h1>';
+ }
+ else if(id == 'd20131011')
+ {
+  ds ='<h1>European Jamboree Lyon start</h1>';
+ }
+ else if(id == 'd2013104')
+ {
+  ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';
+ }
+      else if(id == 'd2013102')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Sequencing preparation:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br></div>'
+ }
+       else if(id == 'd2013101')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs </h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Screening PCR:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Results</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2: <b>Growing</b><br>Lb13. pJP059: <b>Growing</b><br>420. pSBLbE-CP11-amilCP: Not growing<br>435. pSBLbC-CP29-amilCP: Not growing<br>440. pSBLbC-red: Not growing<br>441. pSBLbE-red: <b>Growing</b><br>All negative controls were clear, and the positive controls grew</b>.<br></div>'
+ }
+       else if(id == 'd2013928')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1><br><b>Name of participants:</b> Viktor Blomkvist, Mikael Strandgren, Erik Edlund<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><b>L. reuteri:</b><br>Lb3. pVS2<br><br><b>Lc. lactis:</b><br>Lb13. pJP059<br><br><h2>Todays work</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2<br>Lb13. pJP059<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013927')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Overnight:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br><br><b>Streak from frozen stock (for photos):</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013924')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br><br><h2>Results</h2><br><b>Transformation</b>:<br>No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday.<br></div>'
+ }
+       else if(id == 'd2013921')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist <br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Transformation</b><br><i>To L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2 (positive control)<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>Erythromycin<br>Chloramphenicol <br>Antibiotic-free<br></div>'
+ }
+       else if(id == 'd2013920')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
   else if(id == 'd2013919')
   {
    ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';
   }
+       else if(id == 'd2013918')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1><br><b>Name of participants: </b> Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>O/N:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+        else if(id == 'd2013917')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+         else if(id == 'd2013916')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>440. pSBLbC-red<br><br><b>Assembly and transformation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
   else
   {

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Latest revision as of 23:26, 4 October 2013