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Team:Washington/M9Plate
From 2013.igem.org
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| - | 1. Make M9 + | + | 1. Make <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a></br> |
| - | 2. Add 1g | + | 2. Add 1g of <a href = "http://www.lifetechnologies.com/order/catalog/product/16520050">Low Melt Agarose</a> per 100ml media. </br> |
3. Autoclave the mixture.</br> | 3. Autoclave the mixture.</br> | ||
| - | 4. When solution has cooled down to | + | 4. When solution has cooled down to 42C add appropriate antibiotic.</br> |
</br> | </br> | ||
Latest revision as of 20:48, 27 September 2013
M9 + Casamino Acid Plate 1. Make M9 + Casamino Acid Media 2. Add 1g of Low Melt Agarose per 100ml media. 3. Autoclave the mixture. 4. When solution has cooled down to 42C add appropriate antibiotic.
