Team:Washington/LightIntensityTest

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<b><u>Light Intensity Test</u></b>
<b><u>Light Intensity Test</u></b>
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1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain an overnight culture listed above.</br>
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2. Follow the protocol to run a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-well test</a>, setting up the E. colight app as follows, wells are set to a varying intensity from 100% to 0%, decreasing by 20% for every other well. For a clear setup, check the supplemental image. (link and picture relevant to it)</br>
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1. Follow the protocol to run a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-well test</a>, <a href = "https://2013.igem.org/Team:Washington/appsetup#Intensity">setting up the E. colight app</a> as follows: </br>
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3. Run <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>
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-Intensity: 100%</br>
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-Intensity: 80%</br>
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-Intensity: 60%</br>
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-Intensity: 40%</br>
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-Intensity: 20%</br>
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-Intensity: 0%</br>
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For a clear setup, check the supplemental image below.</br>
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[[Image:Washington_app_intensity1.jpg|border||300px|left|thumb|]]
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[[Image:Washington_app_intensity2.jpg|border||300px|left|thumb|]]
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</br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br>
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2. Run <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>
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Latest revision as of 21:25, 27 September 2013


Light Intensity Test
1. Follow the protocol to run a 96-well test, setting up the E. colight app as follows:
-Intensity: 100%
-Intensity: 80%
-Intensity: 60%
-Intensity: 40%
-Intensity: 20%
-Intensity: 0%
For a clear setup, check the supplemental image below.

Washington app intensity1.jpg

Washington app intensity2.jpg
























2. Run Fluorescent Analysis Protocol.