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Team:Washington/BleedThroughTest
From 2013.igem.org
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2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate</a> setup with these settings:</br> | 2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate</a> setup with these settings:</br> | ||
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br> | - Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br> | ||
| - | - Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. | + | - Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. </br> |
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| + | [[Image:Washington_app_bleed1.jpg|border||300px|left|thumb|]] | ||
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| + | </html> | ||
| + | [[Image:Washington_app_bleed2.jpg|border||300px|left|thumb|]] | ||
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| + | </br> | ||
| + | </br></br></br></br></br></br></br></br></br></br></br></br></br>Note: Addidtional lit wells on the columns serves as replicas. </br> | ||
3. Align the 96-well Plate to the wells on the app and then tape them down securely. | 3. Align the 96-well Plate to the wells on the app and then tape them down securely. | ||
</br> | </br> | ||
Latest revision as of 21:36, 27 September 2013
Bleed-Through Test 1. Follow the general protocol to obtain inoculated culture. 2. Follow the protocol for a 96-Well Plate setup with these settings: - Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait. - Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait.
Note: Addidtional lit wells on the columns serves as replicas. 3. Align the 96-well Plate to the wells on the app and then tape them down securely.
